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Comparing YOLOv8 and Mask R-CNN for instance segmentation in complex orchard environments

Artificial Intelligence in Agriculture 2024年第3期13卷 84-99页

作者： Ranjan Sapkota Dawood Ahmed Manoj Karkee center for Precision&Automated Agricultural Systems Washington State University24106 N Bunn RdProsser99350 WashingtonUSA

Instance segmentation,an important image processing operation for automation in agriculture,is used to precisely delineate individual objects of interestwithin images,which provides foundational information for various automated or robotic tasks such as selective harvesting and precision *** study compares the one-stage YOLOv8 and the two-stage Mask R-CNN machine learning models for instance segmentation under varying orchard conditions across two *** 1,collected in dormant season,includes images of dormant apple trees,which were used to train multi-object segmentation models delineating tree branches and *** 2,collected in the early growing season,includes images of apple tree canopies with green foliage and immature(green)apples(also called fruitlet),which were used to train single-object segmentation models delineating only immature green *** results showed that YOLOv8 performed better than Mask R-CNN,achieving good precision and near-perfect recall across both datasets at a confidence threshold of ***,for Dataset 1,YOLOv8 achieved a precision of 0.90 and a recall of 0.95 for all *** comparison,Mask R-CNN demonstrated a precision of 0.81 and a recall of 0.81 for the *** Dataset 2,YOLOv8 achieved a precision of 0.93 and a recall of *** R-CNN,in this single-class scenario,achieved a precision of 0.85 and a recall of ***,the inference times for YOLOv8 were 10.9 ms for multi-class segmentation(Dataset 1)and 7.8 ms for single-class segmentation(Dataset 2),compared to 15.6 ms and 12.8 ms achieved by Mask R-CNN's,*** findings showYOLOv8's superior accuracy and efficiency in machine learning applications compared to two-stage models,specifically Mask-R-CNN,which suggests its suitability in developing smart and automated orchard operations,particularly when real-time applications are necessary in such cases as robotic harvesting and robotic immature green fruit thin

关键词： YOLOv8 Mask R-CNN Deep learning Machine learning Automation Robotics Artificial intelligence Machine vision

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CD137 agonist potentiates the abscopal efficacy of nanoparticle-based photothermal therapy for melanoma

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Nano Research 2022年第3期15卷 2300-2314页

作者： Preethi Bala Balakrishnan Debbie KLedezma Juliana Cano-Mejia Jaclyn Andricovich Erica Palmer Vishal APatel Patricia SLatham Eric SYvon Alejandro Villagra Rohan Fernandes Elizabeth E.Sweeney GW Cancer center Department of MedicineSchool of Medicine and Health SciencesGeorge Washington UniversityWashingtonDC 20052USA The Institute for Biomedical Sciences School of Medicine and Health SciencesGeorge Washington UniversityWashingtonDC 20052USA GW Cancer center Department of Biochemistry and Molecular MedicineSchool of Medicine and Health SciencesGeorge Washington UniversityWashingtonDC 20052USA Department of Dermatology&Oncology School of Medicine and Health SciencesGeorge Washington UniversityWashingtonDC 20037USA Department of Pathology School of Medicine and Health SciencesGeorge Washington UniversityWashingtonDC 20037USA ImmunoBlue BethesdaMD 20817USA

Despite the promise of immunotherapy such as the immune checkpoint inhibitors(ICIs)anti-PD-1 and anti-CTLA-4 for advanced melanoma,only 26%–52%of patients respond,and many experience grade III/IV immune-related adverse *** by the need for an effective therapy for patients non-responsive to clinically approved ICIs,we have developed a novel nanoimmunotherapy that combines locally administered Prussian blue nanoparticle-based photothermal therapy(PBNP-PTT)with systemically administered agonistic anti-CD137 monoclonal antibody therapy(aCD137).PBNP-PTT was administered at various thermal doses to melanoma cells in vitro,and was combined with aCD137 in vivo to test treatment effects on melanoma tumor progression,animal survival,immunological protection against tumor rechallenge,and *** administered at a melanoma-specific thermal dose,PBNP-PTT elicits immunogenic cell death(ICD)in melanoma cells and upregulates markers associated with antigen presentation and immune cell co-stimulation in ***,PBNP-PTT eliminates primary melanoma tumors in vivo,yielding long-term tumor-free ***,the antitumor immune effects generated by PBNP-PTT cannot eliminate secondary tumors,despite significantly slowing their *** addition of aCD137 enables significant abscopal efficacy and improvement of survival,functioning through activated dendritic cells and tumor-infiltrating CD8^(+)T cells,and generates CD4^(+)and CD8^(+)T cell memory that manifests in the rejection of tumor rechallenge,with no long-term *** study describes for the first time a novel and effective nanoimmunotherapy combination of PBNP-PTT with aCD137 mAb therapy for melanoma.

关键词： photothermal therapy Prussian blue nanoparticles anti-CD137 melanoma nanoimmunotherapy

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Emerging complexity and new roles for the RIG-I-like receptors in innate antiviral immunity

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Virologica Sinica 2015年第3期30卷 163-173页

作者： John S.Errett Michael Gale Jr. center for Innate Immunity and Immune Disease Department of Immunology School of Medicine University of Washington

Innate immunity is critical for the control of virus infection and operates to restrict viral susceptibility and direct antiviral immunity for protection from acute or chronic viral-associated diseases including cancer. RIG-I like receptors(RLRs) are cytosolic RNA helicases that function as pathogen recognition receptors to detect RNA pathogen associated molecular patterns(PAMPs) of virus infection. The RLRs include RIG-I, MDA5, and LGP2. They function to recognize and bind to PAMP motifs within viral RNA in a process that directs the RLR to trigger downstream signaling cascades that induce innate immunity that controls viral replication and spread. Products of RLR signaling also serve to modulate the adaptive immune response to infection. Recent studies have additionally connected RLRs to signaling cascades that impart inflammatory and apoptotic responses to virus infection. Viral evasion of RLR signaling supports viral outgrowth and pathogenesis, including the onset of viral-associated cancer.

关键词： RIG-I MDA5 pattern recognition receptor pathogen associated molecular pattern innate immunity virus infection

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Development of minimally invasive techniques for management of medically-complicated obesity

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World Journal of Gastroenterology 2014年第37期20卷 13424-13445页

作者： Farzin Rashti Ekta Gupta Suzan Ebrahimi Timothy R Shope Timothy R Koch Christopher J Gostout Section of Gastroenterology and Hepatology MedStar-Washington Hospital Center and Georgetown University Schoolof Medicine Department of Surgery MedStar-Washington Hospital CenterCenter for Advanced Laparoscopic and Bariatric Surgery Division of Gastroenterology and Hepatology Mayo Medical School

The field of bariatric surgery has been rapidly growing and evolving over the past several decades. During the period that obesity has become a worldwide epidemic, new interventions have been developed to combat this complex disorder. The development of new laparoscopic and minimally invasive treatments for medically-complicated obesity has made it essential that gastrointestinal physicians obtain a thorough understanding of past developments and possible future directions in bariatrics. New laparoscopic advancements provide patients and practitioners with a variety of options that have an improved safety profile and better efficacy without open, invasive surgery. The mechanisms of weight loss after bariatric surgery are complex and may in part be related to altered release of regulatory peptide hormones from the gut. Endoscopic techniques designed to mimic the effects of bariatric surgery and endolumenal inter-ventions performed entirely through the gastrointestinal tract offer potential advantages. Several of these new techniques have demonstrated promising, preliminary results. We outline herein historical and current trends in the development of bariatric surgery and its transition to safer and more minimally invasive procedures designed to induce weight loss.

关键词： Obesity Bariatrics Bariatric surgery Weight loss Endoscopy

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Advances of Intracranial Electroencephalography in Localizing the Epileptogenic Zone

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Neuroscience Bulletin 2016年第5期32卷 493-500页

作者： Bo Jin Norman K.So Shuang Wang Department of Neurology Epilepsy Center Second Affiliated Hospital School of Medicine Zhejiang University Regional Epilepsy center at Harborview University of Washington

Intracranial electroencephalography（i EEG）provides the best precision in estimating the location and boundary of an epileptogenic zone. Analysis of i EEG in the routine EEG frequency range（0.5-70 Hz） remains the basis in clinical practice. Low-voltage fast activity is the most commonly reported ictal onset pattern in neocortical epilepsy, and low-frequency high-amplitude repetitive spiking is the most commonly reported ictal onset pattern in mesial temporal lobe epilepsy. Recent studies using wideband EEG recording have demonstrated that examining higher（80-1000 Hz） and lower（0.016-0.5 Hz） EEG frequencies can provide additional diagnostic information and help to improve the surgical outcome. In addition,novel computational techniques of i EEG signal analysis have provided new insights into the epileptic ***, we review some of these recent advances. Although these sophisticated and advanced techniques of i EEG analysis show promise in localizing the epileptogenic zone,their utility needs to be further validated in larger studies.

关键词： Epilepsy Intracranial electroencephalography Epileptogenic zone

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Dysphagia after vertical sleeve gastrectomy: Evaluation of risk factors and assessment of endoscopic intervention

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World Journal of Gastroenterology 2016年第47期22卷 10371-10379页

作者： Anand Nath Sayali Yewale Tung Tran John S Brebbia Timothy R Shope Timothy R Koch Department of Medicine Med Star-Washington Hospital Center and Georgetown University School of Medicine Washington DC 20010 United States Unterberg Children’s Hospital at Monmouth Medical center Long Branch NJ07740 United States center for Advanced Laparoscopic General and Bariatric Surgery MedS tar-Washington Hospital Center and Georgetown University School of Medicine Washington DC 20010 United States

AIM To evaluate the risks of medical conditions, evaluate gastric sleeve narrowing, and assess hydrostatic balloon dilatation to treat dysphagia after vertical sleeve gastrectomy(VSG).METHODS VSG is being performed more frequently worldwide as a treatment for medically-complicated obesity, and dysphagia is common post-operatively. We hypothesize that post-operative dysphagia is related to underlying medical conditions or narrowing of the gastric sleeve. This is a retrospective, single institution study of consecutive patients who underwent sleeve gastrectomy from 2013 to 2015. Patients with previous bariatric procedures were excluded. Narrowing of a gastric sleeve includes: inability to pass a 9.6 mm gastroscope due to stenosis or sharp angulation or spiral hindering its *** Of 400 consecutive patients, 352 are included; the prevalence of dysphagia is 22.7%; 33 patients(9.3%) have narrowing of the sleeve with 25(7.1%) having sharp angulation or a spiral while 8(2.3%) have a stenosis. All 33 patients underwent balloon dilatation of the gastric sleeve and dysphagia resolved in 13 patients(39%); 10 patients(30%) noted resolution of dysphagia after two additional dilatations. In a multivariate model, medical conditions associated with post-operative dysphagia include diabetes mellitus, symptoms of esophageal reflux, a low whole blood thiamine level, hypothyroidism, use of non-steroidal anti-inflammatory drugs, and use of *** Narrowing of the gastric sleeve and gastric sleeve stenosis are common after VSG. Endoscopic balloon dilatations of the gastric sleeve resolves dysphagia in 69% of patients.

关键词： Obesity Bariatric surgery Vertical sleeve gastrectomy Dysphagia Stomach dilatation

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Skeletal Blood Flow in Bone Repair and Maintenance

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Bone Research 2013年第4期1卷 311-322页

作者： Ryan E.Tomlinson Matthew J.Silva Department of Orthopaedic Surgery Washington University in St.Louis Musculoskeletal Research center Washington University in St.Louis

Bone is a highly vascularized tissue, although this aspect of bone is often overlooked. In this article, the importance of blood flow in bone repair and regeneration will be reviewed. First, the skeletal vascular anato- my, with an emphasis on long bones, the distinct mechanisms for vascularizing bone tissue, and methods for remodeling existing vasculature are discussed. Next, techniques for quantifying bone blood flow are briefly summarized. Finally, the body of experimental work that demonstrates the role of bone blood flow in fracture healing, distraction osteogenesis, osteoporosis, disuse osteopenia, and bone grafting is examined. These results illustrate that adequate bone blood flow is an important clinical consideration, particularly during bone regeneration and in at-risk patient groups.

关键词： blood flow angiogenesis vascular remodeling fracture bone repair

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Effect of medical and surgical treatment of Barrett's metaplasia

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World Journal of Gastroenterology 2010年第30期16卷 3773-3779页

作者： Eelco B Wassenaar Brant K Oelschlager center for Videoendoscopic Surgery Department of SurgeryUniversity of WashingtonSeattleWA 98195United States

Barrett’s esophagus (BE) is a change in the esophageal mucosa as a result of long-standing gastroesophageal reflux disease. The importance of BE is that it is the main risk factor for the development of esophageal adenocarcinoma, whose incidence is currently growing faster than any other cancer in the Western world. The aim of this review was to compare the common treatment modalities of BE, with the focus on proton pump inhibitors and operative fundoplication. We performed a literature search on medical and surgical treatment of BE to determine eligible studies for this review. Studies on medical and surgical treatment of BE are discussed with regard to treatment effect on progression and regression of disease. Although there is some evidence for control of reflux with either medical or surgical therapy, there is no def initive evidence that either treatment modality decreases the risk of progression to dysplasia or cancer. Even though there is a trend toward antireflux surgery being superior, there are no def initive studies to prove this.

关键词： Barrett’s esophagus Intestinal metaplasia Proton pump inhibitors Nissen fundoplication Esophageal adenocarcinoma Gastroesophageal reflux

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Pregnant inflammatory bowel disease patients may require counselling regarding live vaccines in newborns

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World Journal of Gastroenterology 2014年第33期20卷 11927-11928页

作者： Adith Sekaran Marie L Borum Division of Gastroenterology and Liver Diseases George Washington University Medical Center Washington DC NW 20037 United States

Inflammatory bowel disease patients are prone to immunosuppression due to effects of their medications. Physicians are recommended to assess vaccination status and overall health in all patients, prior to initiation of immunosuppressive therapy. Immunosuppressant medications in women with inflammatory bowel disease are often continued during pregnancy, which can result in newborns having an increased risk of immunosuppression at birth. While medication-induced immunosuppression in infants is transient, parents should be counselled about delaying live vaccine administration in newborns until they are immune competent. A retrospective study was done over six months at an urban multispecialty medical center to assess whether physicians are counselling pregnant immunosuppressed inflammatory bowel disease patients regarding live vaccinations in their newborns. The study revealed that only 57% of patients had documented counselling in their charts. Further studies are necessary to determine physician counselling practices of pregnant women about live vaccines. It is critical that physicians and patients are aware of the risks of immunosuppression in pregnancy and the potential impact of live vaccinesupon the newborn.

关键词： Inflammatory bowel disease Pregnancy Vaccines

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High-Pressure Experimental Studies on Geo-Liquids Using Synchrotron Radiation at the Advanced Photon Source

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Journal of Earth Science 2014年第6期25卷 939-958页

作者： Yanbin Wang Guoyin Shen center for Advanced Radiation Sources The University of Chicago HPCAT Geophysical Laboratory Carnegie Institution of Washington

We review recent progress in studying silicate, carbonate, and metallic liquids of geological and geophysical importance at high pressure and temperature, using the large-volume high-pressure devices at the third-generation synchrotron facility of the Advanced Photon Source, Argonne National Laboratory. These integrated high-pressure facilities now offer a unique combination of experimental techniques that allow researchers to investigate structure, density, elasticity, viscosity, and interfacial tension of geo-liquids under high pressure, in a coordinated and systematic fashion. Experimental techniques are described, along with scientific highlights. Future developments are also discussed.

关键词： high pressure synchrotron melts liquid structure magma dynamics mantle dynamics

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