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Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori

Insect Science 2024年第4期31卷 1041-1054页

作者： Xiaojing Liu Zhongjie Zhang Bo Hu Kai Chen Ye Yu Hui Xiang Anjiang Tan Jiangsu key laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina key laboratory of silkworm and mulberry genetic improvement Ministry of Agriculture and Rural AffairsThe Sericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangJiangsu ProvinceChina Guangzhou key laboratory of Insect Development Regulation and Application Research Institute of Insect Science and Technology and School of Life SciencesSouth China Normal UniversityGuangzhouChina

The diversity of cell types in the brain and how these change during different developmental stages,remains largely *** life cycle of insects is short and goes through 4 distinct stages including embryonic,larval,pupal,and adult *** postembryonic life,the larval brain transforms into a mature adult version after *** silkworm,Bombyx mori,is a lepidopteran model ***,we characterized the brain cell repertoire of larval and adult *** by obtaining 50708 single-cell *** and 12 cell clusters from larval and adult brains were assigned based on marker genes,*** cell types include Kenyon cells,optic lobe cells,monoaminergic neurons,surface glia,and astrocyte *** further assessed the cell type compositions of larval and adult *** found that the transition from larva to adult resulted in great expansion of glial *** glial cell accounted for 49.8%of adult midbrain *** to flies and ants,the mushroom body kenyon cell is insufficient in ***,which accounts for 5.4%and 3.6%in larval and adult brains,*** of neuropeptide expression showed that the abundance and specificity of expression varied among individual ***,we found that ion transport peptide was specifically expressed in glial cells of larval and adult *** cell atlas dataset provides an important resource to explore cell diversity,neural circuits and genetic profiles.

关键词： brain neuropeptide silkworm single-cell transcriptome

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Self-Assembly of Bacteria in Alternating-Current Electric Fields

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Chinese Physics Letters 2024年第10期41卷 137-143页

作者： Yan-Ran Li Yi-Wu Zong Hong Zhang Jing-Chao Zhang Chun-Ying Feng Jian-Jun Qiao Hao Song Kun Zhao Frontiers Science Center for Synthetic Biology and key laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Petrochemical Research Institute of Petrochina Co. Ltd.Beijing 102206China Center for Medical genetics Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 611731China Jiangsu key laboratory of Sericultural and Animal Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang 212100China key laboratory of silkworm and mulberry genetic improvement Ministry of Agriculture and Rural AffairsSericultural Scientific Research CenterChinese Academy of Agricultural SciencesZhenjiang 212100China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China The Sichuan Provincial key laboratory for Human Disease Gene Study and The Institute of laboratory Medicine Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610054China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China

Self-assembly of bacteria in electric fields is a promising route to fabricate biomaterials with reversible and specific ***,due to relatively less studies,our understanding of the self-assembly of bacteria in electric fields is still ***,how different bacterial species behave differently in their fieldmediated self-assembly behavior remains to be *** this study,we choose four bacterial species,including Shewanella oneidensis,Pseudomonas aeruginosa,Bacillus subtilis and Lactococcus lactis as model systems,and investigate their self-assembly behavior in alternating-current(AC)electric fields for both diluted and concentrated *** phase diagrams in the plane of applied field strength vs frequency are *** results show that in diluted suspensions,a transition sequence of isotropic–paranematic–string–columnar phases is observed in all strains as the field strength *** of the assembled structures are quantitatively differentiated among different *** concentrated suspensions,besides the isotropic and paranematic phases,a higher ordered phase with interdigitating rectangular crystal domains(OIR)and an ordered phase with smectic A liquid crystal domains are observed for *** and ***,*** findings shed new light on fabricating potential biomaterials by assembling cells of appropriately chosen bacterial species that have desired surface properties under AC electric fields.

关键词： suspensions diluted biomaterials

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Characterization of mulberry leaf instant tea and evaluation of its hypolipidemia effect via regulation of intestinal microbiota

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Food Science and Human Wellness 2024年第3期13卷 1348-1357页

作者： Xiaoyun Han Yunlong Bai Xiaoxin Feng Baochang Du Baojiang Zheng Qingshen Sun Engineering Research Center of Agricultural Microbiology Technology Ministry of Education&Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold RegionKey Laboratory of Molecular Biology&Key Laboratory of MicrobiologyCollege of Heilongjiang Province&School of Life SciencesHeilongjiang UniversityHarbin 150080China Gannan Forest Farm Qiqihaer 162100China College of Life Sciences Northeast Forestry UniversityHarbin 150040China

The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla *** 1)mulberry leaves in Heilongjiang Province(China)and assess its obesity-preventing/relieving effects.A total of 174 compounds including quercetin,chlorogenic acid,1-deoxyecomycin(1-DNJ)related to antihyperlipidemia effects were identified from the MLIT *** treatment reversed the Lee's index,fat coefficient,and serum biochemical parameters in both the obesity relieving and obesity preventing mice fed with high-fat *** the obesity relieving experiment,the relative abundance of Desulfovibrio in mouse feces decreased after both 0.5%and 1%MLIT *** obesity preventing experiments,mouse with different amount of MLIT treatments showed increased relative abundance of Akkermansia,Bifidobacterium and Lactobacillus,while Deferribacteres,Desulfobacterota *** beneficial bacteria in the intestinal tract of mice treated with MLIT *** study proved that MLIT had antihyperlipidemia potential via modulating intestinal microbiota in mice.

关键词： mulberry leaf instant tea Liquid chromatography-mass spectrometry(LC-MS) Antihyperlipidemia Intestinal microbiota

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mulberry Biomass-Derived Nanomedicines Mitigate Colitis through Improved Inflamed Mucosa Accumulation and Intestinal Microenvironment Modulation

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Research 2024年第1期2023卷 791-807页

作者： Wenjing Yang Ya Ma Haiting Xu Zhenhua Zhu Jiaxue Wu Cheng Xu Wei Sun Erhu Zhao Min Wang Rui L.Reis Subhas C.Kundu Xiaoxiao Shi Bo Xiao State key laboratory of silkworm Genome Biology College of SericultureTextileand Biomass SciencesSouthwest UniversityChongqing 400715China Chongqing key laboratory of Soft-Matter Material Chemistry and Function Manufacturing Faculty of Materials and EnergySouthwest UniversityChongqing 400715China Department of Gastroenterology The First Affiliated Hospital of Nanchang UniversityNanchang 330006China Bs Research Group I3Bs—Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkBarco 4805-017GuimaraesPortugal ICVS/3B’s-PT Government Associate laboratory BragaGuimarãesPortugal

The therapeutic outcomes of conventional oral medications against ulcerative colitis(UC)are restricted by inefficient drug delivery to the colitis mucosa and weak capacity to modulate the inflammatory ***,a fluorinated pluronic(FP127)was synthesized and employed to functionalize the surface of mulberry leaf-derived nanoparticles(MLNs)loading with resveratrol nanocrystals(RNs).The obtained FP127@RN-MLNs possessed exosome-like morphologies,desirable particle sizes(around 171.4 nm),and negatively charged surfaces(−14.8 mV).The introduction of FP127 to RN-MLNs greatly improved their stability in the colon and promoted their mucus infiltration and mucosal penetration capacities due to the unique fluorine effect.

关键词： fluorine sizes Biomass

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Genome-wide analysis of microRNAs induced and regulated by colchicine in mulberry

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BIOCELL 2022年第8期46卷 1935-1945页

作者： MENGMENG WU PENG GUO YISU SHI DANYAN ZHENG QIAONAN ZHANG XIN JIN YUHUA WANG QIANG LIN RONGJUN FANG WEIGUO ZHAO key laboratory of silkworm and mulberry genetic improvement Ministry of AgricultureSchool of Biology and TechnologyJiangsu University of Science and TechnologyZhenjiang212100China Sericultural Research Institute Nanning530007China

A variety of plants were colchicine treated to double their chromosome *** are genetic carriers that determine the individual traits of *** doubling of chromosomes will lead to modifications in plant morphology,physiology and *** determine the response of mulberry trees induced by colchicine,using mulberry variety Yu-711 leaves as research materials,two small RNA libraries(control and experimental groups)were *** was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results.A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA genes,19 genes of which were up-regulated and 18 genes of which were *** miRNAs were selected from 37 differentially expressed miRNA *** miRNAs were verified by quantitative real-time PCR,and the expression levels of these miRNAs were found to be consistent with those obtained by sequencing,which proved the accuracy of sequencing *** PsRNATarget software was used to predict the target genes of 8 *** Gene Ontology and Kyoto Encycopedia of Genes and Genomes analysis were used to collect the functions of target genes and confirm that target genes are mainly involved in biological processes,cell components and molecular ***-77 miRNA was selected from 37 differentially expressed *** will serve as a fundamental data in understanding mulberry miRNAs function in molecular biology research and further provides the molecular mechanism of mulberry gene regulation as induced by colchicine is that the prediction of novel_77 precursor sequence and the target gene(XM_010104258.2),as well as the secondary structure analysis.

关键词： Chromosomes Novel_77 qRT-PCR Target genes Colchicine

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CRISPR/Cas9-based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori

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Insect Science 2021年第5期28卷 1414-1425页

作者： Moses Addo Nartey Xia Sun Sheng Qin Cheng-Xiang Hou Mu-Wang Li Jiangsu key laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsuChina the key laboratory of silkworm and mulberry genetic improvement Ministry of AgricultureSericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangJiangsuChina

Circadian rhythms,which are ubiquitous and adaptive,occur across all species,from microbes to humans,in which they organize and modify behavior and ***(im)is a canonical clock *** core composition of the Drosophila melanogaster endogenous circadian clock has been extensively investigated;however,in lepidopteran insects,including Bombyr mori,the mechanism is complicated and little is known regarding the participation of tim in the negative feedback loop responsible for behavioral *** arrive at a comprehensive understanding of the role of tim in the *** endogenous circadian clock,we exploited the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 gene editing *** attermpted to elucidate the functions of tim in the circadian clock of *** using Bmtim *** knockouts affected two circadian behavioral activities:adult emergence and embryo hatching *** real-time polymerase chain reaction results confirmed that tim-knockouts induced relative reductions in the expression levels,and thereby the oscillation amplitudes,of Bmper and Bmclk messenger RNAs during both the photophase and ***,the daily rhythmic expression of Bmndbt was up-regulated in the photophase and downregulated in the scotophase in a *** study reveals that tim is integral to the *** circadian clock and may be involved in regulating eclosion and hatching rhythms.

关键词： Bombyx mori circadian trhythm CRISPR/Cas9 eclosion hatching JKT_CYCLE timeless

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BmSd gene regulates the silkworm wing size by affecting the Hippo pathway

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Insect Science 2020年第4期27卷 655-664页

作者： Jin Yin Jing Zhang Tao Li Xia Sun Sheng Qin Cheng-Xiang Hou Guo-Zheng Zhang Mu-Wang Li Jiangsu key laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsuChina the key laboratory of silkworm and mulberry genetic improvement Ministry of AgricultureThe Sericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangJiangsuChina

Insect wings are developed from the wing disc during *** mori,a model lepidopteran insect,loses flight ability after long-term domestication from the wild silkworm,Bombyx *** mw mutant(ul 1 strain)shows minute wings compared to wild type(e.g.,p50 strain)*** sequencing analysis previously revealed differential Hippo-pathway-related gene expression between the ull and *** Hippo pathway is an evolutionarily conserved signaling cascade that controls organ size during development in *** this study,the function of BmSd which has been characterized as one of the Hippo-pathway-related genes was analyzed for silkworm wing *** found that mats,warts,and hippo expression levels were higher in u11 compared to p50 wing ***(scalloped)expression,which encodes a prominent transcriptional partner to Yorkie(Yki),gradually decreased during the wandering stage in ull,but exhibited the opposite expression pattern in *** BmSd was knocked down by small interfering RNA during the wandering stage in the p50 strain,57.9%of the individuals showed minute ***,ex,kibras and wingless expression levels decreased in the BmSd knockdown ***,BmSd deletion mediated by clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 induced 50%of individuals with minute wings,a phenotype similar to the mw *** result demonstrates that BmSd plays pivotal roles in silkworm wing *** results show that the Hippo signaling pathway participates and plays crucial roles in the regulation of silkworm wing development,and our findings provide a basis for further research on *** wing development.

关键词： BmSd Bombyx mori Hippo pathway metamorphosis wing disc

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Flavonoids from mulberry leaves inhibit fat production and improve fatty acid distribution in adipose tissue in finishing pigs

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Animal Nutrition 2024年第1期16卷 147-157页

作者： Yingying Liu Yinglin Peng Chen Chen Huibo Ren Ji Zhu Yuan Deng Qingming Cui Xionggui Hu Jianhua He Huali Li Xinghui Zhu Yulong Yin Jun He Yi Xiao College of Animal Science and Technology Hunan Agricultural UniversityChangsha410128China Hunan Institute of Animal and Veterinary Science Changsha410131China key laboratory of Conservation and genetic Analysis of Local Pig Breed Germplasm Resources Changsha410131China College of Information and Intelligence Hunan Agricultural UniversityChangsha410128China Institute of Subtropical Agriculture Chinese Academy of SciencesChangsha410125China

This study evaluated the effects of flavonoids from mulberry leaves(FML)on plasma biochemical indices,serum activities of lipid metabolism-related enzymes,fat morphology,fatty acid composition,and lipid metabolism in different adipose tissues of finishing *** used 120 Chinese hybrid barrows of Berkshire and Bama mini-pigs with an average initial body weight of 45.11±4.23 *** pigs were randomly assigned to five treatment groups and fed a control diet based on corn,soybean meal,and wheat bran or a control diet supplemented with 0.02%,0.04%,0.08%,or 0.16%*** experimental group had six replicates(pens),with four pigs per *** a 7-d adaptation period,the feeding trial was conducted for 58 *** and adipose tissue samples were collected from 30 pigs(one pig per pen)at the end of the *** results showed that FML supplementation significantly decreased the feed intake to body gain ratio,the plasma concentrations of total cholesterol and free fatty acids,and the serum activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase(linear or quadratic effects,P<0.05),and decreased the plasma triglyceride concentration(quadratic,P=0.07).Increasing FML supplementation increased the average daily gain and serum activities of lipoprotein lipase(linear and quadratic effects,P<0.05)and adipose triglyceride lipase(linear,P<0.05).Dietary FML supplementation decreased the adipocyte area in the dorsal subcutaneous adipose(DSA)tissue of finishing pigs(linear,P=0.05)and increased the adipocyte area in the visceral adipose tissue(quadratic,P<0.01).Increasing FML supplementation decreased the C20:1 content in DSA,abdominal subcutaneous adipose,and visceral adipose tissues of finishing pigs(P<0.05)and increased the C18:3n3 and n-3 PUFA contents(P<0.05).The lipid metabolism genes were regulated by the PPARγ-LXRa-ABCA1 signaling pathway,and their expressions differed in different adipose *** findings suggest that FML improved growth performance,regulated lipid met

关键词： Finishing pig mulberry leaf Flavonoid Lipid metabolism Fatty acid Lard

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A study of miRNAs targets prediction and experimental validation

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Protein & Cell 2010年第11期1卷 979-986页

作者： Yong Huang Quan Zou Haitai Song Fei Song Ligang Wang Guozheng Zhang Xingjia Shen Jiang Su University of Science and Technology Zhenjiang 212018China School of information Science and Technology of Xiamen University Xiamen 361005China the key laboratory of silkworm and mulberry genetic improvement Ministry of AgricultureSericultural Research InstituteChinese Academy of Agricultural SciencesZhejiang 212018China

microRNAs(miRNAs)are 20–24 nucleotide(nt)RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs(mRNAs).To identify miRNA target genes will help a lot by understanding their biological *** computational approaches for miRNA target prediction,and effective biological techniques for validating these targets now play a central role in elucidating their *** to the imperfect complementarity of animal miRNAs with their targets,it is difficult to judge the accuracy of the *** of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the *** date,only a few miRNAs targets are *** this article,we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals.

关键词： microRNA computational prediction target experimental validation

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Expression and Activity Analysis of Non-Structural Protein 2 (NS2) of Bombyx mori Densovirus Zhenjiang Strain

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Agricultural Sciences in China 2010年第12期9卷 1821-1828页

作者： ZHAO Pan TANG Shun-ming LIU Tingx LIU Ting QIN Guang-xing GUO Xi-j ie College of Biotechnology and Environmental Engineering Jiangsu University of Science and Technology Zhenjiang 212003 P.R.China key laboratory of silkworm and mulberry genetic improvement Ministry of Agriculture of China/Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang 212018 P.R.China

The gene of the non-structure protein 2 （NS2） was cloned by PCR from the genome ofBombyx mori densovirus Zhenjiang strain （BmDNV-Z）, inserted into prokaryotic expression vector pET28a to construct recombinant plasmid pET28a-NS2 and then expressed in bacteria Escherichia coli BL21 （DE3）. The expressed recombinant protein was identified by SDS-PAGE and Western blot analysis. Then, the recombinant protein was purified by Ni-NTA column, renatured and tested for enzyme activities. The purified NS2 protein exhibited a helicase activity unwinding double-stranded DNA substrates into single-strand primers, and higher unwinding activity to polarity substrate. Similarly, the purified NS2 protein possessed an ATPase activity and its enzyme activity was 0.276 μmol gg^-1 h^-1 in this study. The results indicated that the non- structure protein which encoded by the gene of BmDNV-Z NS2 possesses the biological activities of helicase and ATPase, and the helicase prefers to polarity substrates. Based on these results, it is speculated that the gene of BmDNV-Z NS2 plays an important role in the viral DNA replication.

关键词： Bombyx mori densovirus non-structure protein expression activity

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