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Acta Pharmaceutica Sinica B 2015年第1期5卷 1-7页

作者： Jun Han Pengfei Wu Fang Wang Jianguo Chen Department of Pharmacology School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology laboratory of Neuropsychiatric Diseases The Institute of Brain Research Huazhong University of Science and Technology key laboratory of Neurological Diseases (HUST) Ministry of Education of China the key laboratory for drug target researches and pharmacodynamic evaluation of hubei province

Glutamate acting on AMPA-type ionotropic glutamate receptor(AMPAR) mediates the majority of fast excitatory synaptic transmission in the mammalian central nervous system. Dynamic regulation of AMPAR by post-translational modifications is one of the key elements that allow the nervous system to adapt to environment stimulations. S-palmitoylation, an important lipid modification by post-translational addition of a long-chain fatty acid to a cysteine residue, regulates AMPA receptor trafficking, which dynamically affects multiple fundamental brain functions, such as learning and memory. In vivo, S-palmitoylation is controlled by palmitoyl acyl transferases and palmitoyl *** this review, we highlight advances in the mechanisms for dynamic AMPA receptors palmitoylation,and discuss how palmitoylation affects AMPA receptors function at synapses in recent *** regulation of S-palmitoylation may serve as a novel therapeutic strategy for neurobiological diseases.

关键词： Palmitoylation AMPA receptors Trafficking DHHC

Microglia:A Central Player in Depression

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Current Medical Science 2020年第3期40卷 391-400页

作者： Si-long DENG Jian-guo CHEN Fang WANG Department of Pharmacology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China the key laboratory for drug target researches and pharmacodynamic evaluation of hubei province Wuhan 430030China Tongji-Rongcheng Center for Biomedicine Huazhong University of Science and TechnologyWuhan 430030China

Microglia are the major immune cells in the central nervous system and play a key role in the normal function of the *** exhibit functional diversity,and they control the inflammation in central nervous system through releasing inflammatory cytokine,clearing apoptotic cells via phagocytosis,regulating synaptic plasticity and the formation of neural network by synapse *** studies have strongly indicated that the microglial dysfunction is associated with a variety of neuropsychiatric diseases such as depression,which have been termed as“microgliopathy”.The emergency of advanced technologies and tools has enabled us to comprehensively understand the role of microglia in physiology and pathology,and growing studies have targetted microglia to explore the treatment of neuropsychiatric ***,we describe the key progress of microglia research,and review the recent developments in the understanding of the role of microglia in physiology and etiology of depression.

关键词： microglia central nervous system development adult depression

targeting redox-altered plasticity to reactivate synaptic function: A novel therapeutic strategy for cognitive disorder

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Acta Pharmaceutica Sinica B 2021年第3期11卷 599-608页

作者： Pei Wang Fang Wang Lan Ni Pengfei Wu Jianguo Chen Department of Pharmacology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China key laboratory of Neurological Diseases(HUST) Ministry of Education of ChinaWuhan 430030China the key laboratory for drug target researches and pharmacodynamic evaluation of hubei province Wuhan 430030China laboratory of Neuropsychiatric Diseases the Institute of Brain ResearchHuazhong University of Science and TechnologyWuhan 430030China

Redox-altered plasticity refers to redox-dependent reversible changes in synaptic plasticity via altering functions of key proteins, such as N-methyl-D-aspartate receptor(NMDAR). Age-related cognitive disorders includes Alzheimer’s disease(AD), vascular dementia(VD), and age-associated memory impairment(AAMI). Based on the critical role of NMDAR-dependent long-term potentiation(LTP) in memory, the increase of reactive oxygen species in cognitive disorders, and the sensitivity of NMDAR to the redox status, converging lines have suggested the redox-altered NMDAR-dependent plasticity might underlie the synaptic dysfunctions associated with cognitive disorders. In this review, we summarize the involvement of redox-altered plasticity in cognitive disorders by presenting the available evidence. According to reports from our laboratory and other groups, this "redox-altered plasticity" is Hydrogen sulfidemore similar to functional changes rather than organic injuries, and strategies targeting redox-altered plasticity using pharmacological agents might reverse synaptic dysfunctions and memory abnormalities in the early stage of cognitive disorders. targeting redox modifications for NMDARs may serve as a novel therapeutic strategy for memory deficits.

关键词： Reactive oxygen species N-Methyl-D-aspartate receptor Oxidative stress Synaptic plasticity Long-term potentiation Cognitive disorder Learning and memory Hydrogen sulfide

Identification and Function of Acid-sensing Ion Channels in RAW 264.7 Macrophage Cells

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Current Medical Science 2018年第3期38卷 436-442页

作者： Lan NI Peng FANG Zhuang-li HU Hai-yun ZHOU Jian-guo CHEN Fang WANG You JIN Department of Pharmacology School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China key laboratory of Neurological Diseases (HUST) Ministry of Education of China key laboratory for drug target researches and pharmacodynamic evaluation of hubei province Institute of Brain Research School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Activation of acid-sensing ion channels （ASICs） plays an important role in neuroinflammation. Macrophage recruitment to the sites of inflammation is an essential step in host defense. ASIC1 and ASIC3 have been reported to mediate the endocytosis and maturation of bone marrow derived macrophages. However, the expression and inflammation-related functions of ASICs in RAW 264.7 cells, another common macrophage, are still elusive. In the present study, we first demonstrated the presence of ASIC 1, ASIC2a and ASIC3 in RAW 264.7 macrophage cell line by using reverse transcriptase polymerase chain reaction （RT-PCR）, Western blotting and immunofluorescence experiments. The non-specific ASICs inhibitor amiloride and specific homomeric ASICla blocker PcTxl reduced the production of iNOS and COX-2 by LPS-induced activating RAW 264.7 cells. Furthermore, not only amiloride but also PcTxl inhibited the migration and LPS-induced apoptosis of RAW 264.7 cells. Taken together, our findings suggest that ASICs promote the inflammatory response and apoptosis of RAW 264.7 cells, and ASICs may serve as a potential novel target for immunological disease therapy.

关键词： acid-sensing ion channels (ASICs) RAW 264.7 cells inflammation apoptosis migration

S-methyl-L-cysteine Protects against Antimycin A-induced Mitochondrial Dysfunction in Neural Cells via Mimicking Endogenous Methionine-centered Redox Cycle

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Current Medical Science 2020年第3期40卷 422-433页

作者： Lan NI Xin-lei GUAN Fu-feng CHEN Peng-fei WU Department of Pharmacology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pharmacy Wuhan Puai HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430033China Tongji-Rongcheng Center for Biomedicine Wuhan 430030China key laboratory for drug target researches and pharmacodynamic evaluation of hubei province Wuhan 430030China

Mitochondrial superoxide overproduction is believed to be responsible for the neurotoxicity associated with ***-targeted antioxidants,such as MitoQ,have emerged as potentially effective antioxidant *** sulfoxide reductase A(MsrA)is a key mitochondrial-localized endogenous antioxidative enzyme and it can scavenge oxidizing species by catalyzing the methionine(Met)-centered redox cycle(MCRC).In this study,we observed that the natural L-Met acted as a good scavenger for antimycin A-induced mitochondrial superoxide overproduction in PC12 *** antioxidation was largely dependent on the Met oxidase activity of MsrA.S-methyl-L-cysteine(SMLC),a natural analogue of Met that is abundantly found in garlic and cabbage,could activate the Met oxidase activity of MsrA to scavenge free ***,SMLC protected against antimycin A-induced mitochondrial membrane depolarization and alleviated 1-methyl-4-phenylpyridinium(MPP+)-induced ***,our data highlighted the possibility for SMLC supplement in the detoxication of mitochondrial damage by activating the Met oxidase activity of MsrA.

关键词： methionine sulfoxide reductase A Met oxidase S-methyl-L-cysteine neurotoxin 1-methyl-4-phenylpyridinium

Thymosinβ4 Impeded Murine Stem Cell Proliferation with an Intact Cardiovascular Differentiation

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第3期36卷 328-334页

作者：聂丽高仕君赵亚楠 Jacob Masika 骆红艳胡新武张亮品曾莹 Jürgen Hescheler 梁华敏 Department of Physiology Chinese-German Stem Cell Centerthe Key Laboratory of Drug Target Research and Pharmacodynamic EvaluationHubei Province School of Basic Medicine Huazhong University of Science and Technology Institute of Brain Research Huazhong University of Science and Technology College of Pharmacy Wuhan Institute of Bioengineering Department of Medical Physiology Faculty of Health SciencesEgerton University Union Hospital Huazhong University of Science and Technology Institute of Neurophysiology University of Cologne

Thymosin β4（Tβ4） is a key factor in cardiac development, growth, disease, epicardial integrity, blood vessel formation and has cardio-protective properties. However, its role in murine embryonic stem cells（m ESCs） proliferation and cardiovascular differentiation remains unclear. Thus we aimed to elucidate the influence of Tβ4 on m ESCs. target genes during m ESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the m ESCs-derived cardiomyocytes. It was found that Tβ4 decreased m ESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun. However, m ESCs self-renewal markers Oct4 and Nanog were elevated, indicating the maintenance of self-renewal ability in these m ESCs. Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced. No significant difference was found in BMP2/BMP4 or their downstream protein smad. Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant ？-catenin. Under m ESCs differentiation, Tβ4 treatment did not change the expression of cardiovascular cell markers α-MHC, PECAM, and α-SMA. Neither the electrophysiological properties of m ESCs-derived cardiomyocytes nor the hormonal regulation by Iso/Cch was affected by Tβ4. In conclusion, Tβ4 suppressed m ESCs proliferation by affecting the activity of STAT3, Akt, ERK and Wnt pathways. However, Tβ4 did not influence the in vitro cardiovascular differentiation.

关键词： thymosin β4 murine embryonic stem cells proliferation cardiogenesis patch clamp

Cancer cell derived immunoglobulin G activates platelet via binding platelet FcγRIIa

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Cancer cell derived immunoglobulin G activates platelet via ...

Dimethyl sulfide,a metabolite of the marine microorganism,protects SH-SY5Y cells against 6-hydroxydopamine and MPP~＋-induced apoptosis

首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会

作者：明章银 Shuo Miao Dan Shu Department of Pharmacology School of Basic MedicineTongji Medical College of Huazhong University of Science and Technology The key laboratory for drug target Research and pharmacodynamic evaluation of hubei province The Institute of Brain Research Huazhong University of Science and Technology

Tumor associated thrombosis is the second leading risk factor for death in cancer *** abnormal platelet activity is closely related to *** order to prevent and treat tumor-related thrombosis,it is urgent to investigate the mechanism of abnormal platelet activity in tumor *** is reported that immunoglobulin G(IgG) derived from tumor can regulate the development of ***,the research on cancer derived IgG is very *** studied the role of cancer-derived

关键词： Cancer cell derived immunoglobulin G activates platelet via binding platelet Fc

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中国药理学与毒理学杂志 2017年第10期31卷 1004-1004页

作者： Peng-fei WU Xin-lei GUAN Han LUO Fang WANG Jian-Guo CHEN Department of Pharmacology School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China School of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China key laboratory of Neurological Diseases (HUST) Ministry of Education of China Wuhan 430030 China the key laboratory for drug target researches and pharmacodynamic evaluation of hubei province Wuhan 430030 China

Dimethyl sulfide(DMS)has been historically recognized as a metabolite of the marine microorganism or a disgusting component for the smell of halitosis *** our recent study,DMS has been identified as a cytoprotectant that protects against oxidative-stress induced cell death and *** found that at near-physiological concentrations,DMS reduced reactive oxygen species(ROS)in cultured PC12 cells and alleviated oxidative *** radical-scavenging capacity of DMS at near-physiological concentration was equivalent to endogenous methionine(Met)-centered antioxidant *** sulfoxidereductase A(MsrA),the key antioxidant enzyme in Met-centered defense,bound to DMS and promoted its antioxidant capacity via facilitating the reaction of DMS with ROS through a sulfonium intermediate at residues Cys72,Tyr103,Glu115,followed by the release of dimethyl sulfoxide(DMSO).MTT assay and trypan blue test indicated that supplement of DMS exhibited cytoprotection against 6-hydroxydopamine and MPP+induced cell ***,Msr A knockdown abolished the cytoprotective effect of DMS at near-physiological *** present study reveals new insight into the potential therapeutic value of DMS in Parkinson disease.

关键词： dimethyl sulfide Parkinson disease methionine sulfoxidereductase A 6-hydroxydopamine

Acquired immune thrombotic thrombocytopenic purpura(TTP)associated with inactivated COVID-19 vaccine CoronaVac

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Frontiers of Medicine 2024年第4期18卷 744-751页

作者： Zhangbiao Long Suyu Jiang Honglei Xin Lu Zhang Ruinan Lu Fengqi Liu Yong Xu Linv Wang Jun Wang Xuezhong Zhang Hui Liao Jinning Shi Xue Yan Xiang Zhu Ruonan Shao Zijian Li Yilin Zhu Han Yan Jiao Wu Chao Fang Xiaodong Xi Xiaofeng Shi Department of Hematology The First Affiliated Hospital of Anhui Medical UniversityHefei230022China Department of Hematology The Second Affiliated Hospital of Nanjing Medical UniversityNanjing210003China Department of Hematology The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China Department of Hematology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008China Department of Hematology The Affiliated Hospital of Nanjing University of Chinese MedicineThe First Clinical Medical College of Nanjing University of Chinese MedicineNanjing210009China Department of Hematology Zhongda Hospital of Southeast UniversityNanjing210044China Department of Hematology Nanjing First HospitalNanjing Medical UniversityNanjing210006China Department of Hematology The Air Force Hospital of Eastern Theater commandNanjing210016China Department of Hematology Jiangning Hospital Affiliated of Nanjing Medical UniversityNanjing211199China Department of Hematology Nanjing Hospital of Chinese MedicineNanjing210006China Department of Pharmacology School of Basic MedicineTongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious DiseasesHuazhong University of Science and TechnologyThe Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei ProvinceWuhan430030China Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China

Corona virus disease 2019(COVID-19)due to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection has affected the whole *** thrombotic thrombocytopenic purpura(TTP)has been reported after administration of mRNA-or adenoviral vector-based COVID-19 vaccines,including ***2-S,BNT162b2,mRNA-1273,and ChAdOx1 ***,whether inactivated vaccines,such as CoronaVac,could cause TTP and whether the symptoms in TTPs caused by inactivated vaccines are different from previously reported cases are *** this study,two cases were *** cases developed TTP after the second CoronaVac vaccination shot,but not the *** demonstrated symptoms of fever,neurological abnormalities,renal dysfunction,thrombocytopenia,and *** patients achieved complete remission through several sessions of plasma exchanges and immune *** incidence of TTP in Nanjing area was *** number of patients with TTP was 12 in 2019,6 in 2020,16 in 2021,and 19 in *** the authors’knowledge,this report is the first report of TTP associated with inactivated COVID-19 vaccine(CoronaVac).The rarity and delayed onset may be due to the relatively milder immune response caused by the inactivated vaccines than mRNA-based *** plasma exchange is a vital treatment for CoronaVac-related TTP,similar to activated vaccine-related TTP.

关键词： thrombotic thrombocytopenia purpura(TTP) corona virus disease 2019(COVID-19) CoronaVac acute respiratory syndrome coronavirus 2(SARS-CoV-2) vaccine

Blockade of HCN2 Channels Provides Neuroprotection Against Ischemic Injury via Accelerating Autophagic Degradation in Hippocampal Neurons

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Neuroscience Bulletin 2020年第8期36卷 875-894页

作者： Cheng Chen Li Liu Ya-Qiao Shu Ping Jing Yun Lu Xiao-Xue Zhang Xian-Gang Zong Lian-Jun Guo Chang-Jun Li Department of Pharmacology School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Neurology The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Office of Academic Research The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pharmacy Zhongnan Hospital of Wuhan UniversityWuhan 430030China Department of Clinical laboratory Wuhan PuAi HospitalWuhan 430033China key laboratory of drug target Research and pharmacodynamic evaluation Wuhan 430030China Center for Integrated Protein Science and Zentrum fur Pharmaforschung Department PharmazieLudwig-Maximilians-Universitat Miinchen80539 MunichGermany

In the central nervous system,hyperpolarizationactivated cyclic nucleotide-gated(HCN)channels are essential to maintain normal neuronal *** studies have shown that HCN channels may be involved in the pathological process of ischemic brain injury,but the mechanisms remain *** is activated in cerebral ischemia,but its role in cell death/survival remains *** this study,our results showed that the HCN channel blocker ZD7288 remarkably decreased the percentage of apoptotic neurons and corrected the excessive autophagy induced by oxygen-glucose deprivation followed by reperfusion(OGD/R)in hippocampal HT22 ***,in the OGD/R group,p-mTOR,p-ULK1(Ser757),and p62 were significantly decreased,while p-ULK1(Ser317),atg5,and beclin1 were remarkably ***7288 did not change the expression of p-ULK1(Ser757),ULK1(Ser317),p62,Beclin1,and atg5,which are involved in regulating autophagosome ***,we found that OGD/R induced a significant increase in Cathepsin D expression,but not *** with ZD7288 at 10μmol/L in the OGD/R group did not change the expression of cathepsin D and ***,chloroquine(CQ),which decreases autophagosome-lysosome fusion,eliminated the correction of excessive autophagy and neuroprotection by ***,shRNA knockdown of HCN2 channels significantly reduced the accumulation of LC3-Ⅱand increased neuron survival in the OGD/R and transient global cerebral ischemia(TGCI)models,and CQ also eliminated the effects of ***,we found that the percentage of LC3-positive puncta that co-localized with LAMP-1-positive lysosomes decreased in Con-shRNAtransfected HT22 neurons exposed to OGD/R or *** HCN2-shRNA-transfected HT22 neurons,the percentage of LC3-positive puncta that co-localized with LAMP-1-positive lysosomes increased under OGD/R;however,the percentage was significantly decreased by the addition of CQ to HCN2-shRNA-transfected HT22 *** present re

关键词： HCN2 channel Autophagy Neuroprotection Oxygen-glucose deprivation/reperfusion Transient global cerebral ischemia