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Neural Regeneration Research 2024年第5期19卷 1150-1155页

作者： Shuanglong Yi Linfang Wang Margaret S.Ho Shiping Zhang School of life Science and technology ShanghaiTech UniversityShanghaiChina college of life sciences University of Chinese Academy of SciencesBeijingChina

Parkinson’s disease is a progressive neurodegenerative disease characterized by motor deficits,dopaminergic neuron loss,and brain accumulation ofα-synuclein aggregates called Lewy *** in protein degradation pathways,such as autophagy,has been demonstrated in neurons as a critical mechanism for eliminating protein aggregates in Parkinson’s ***,it is less well understood how protein aggregates are eliminated in glia,the other cell type in the *** the present study,we show that autophagy-related gene 9(Atg9),the only transmembrane protein in the autophagy machinery,is highly expressed in Drosophila glia from adult *** from immunostaining and live cell imaging analysis reveal that a portion of Atg9 localizes to the trans-Golgi network,autophagosomes,and lysosomes in ***9 is persistently in contact with these *** glial atg9 reduces the number of omegasomes and autophagosomes,and impairs autophagic substrate *** suggests that glial Atg9 participates in the early steps of autophagy,and hence the control of autophagic ***,loss of glial atg9 induces parkinsonian symptoms in Drosophila including progressive loss of dopaminergic neurons,locomotion deficits,and glial *** findings identify a functional role of Atg9 in glial autophagy and establish a potential link between glial autophagy and Parkinson’s *** results may provide new insights on the underlying mechanism of Parkinson’s disease.

关键词： Atg9 autophagy glia Parkinson’s disease

Density functional theory study of B- and Si-doped carbons and their adsorption interactions with sulfur compounds

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Carbon Energy 2024年第2期6卷 195-208页

作者： Peng Guo Hong Zhang Shuliang Dong Libao An college of Mechanical Engineering North China University of Science and TechnologyTangshanHebeiChina college of life sciences North China University of Science and TechnologyTangshanHebeiChina

Understanding the adsorption interactions between carbon materials and sulfur compounds has far-reaching impacts,in addition to their well-known important role in energy storage and conversion,such as lithium-ion *** this paper,properties of intrinsic B or Si single-atom doped,and B-Si codoped graphene(GR)and graphdiyne(GDY)were investigated by using density functional theory-based calculations,in which the optimal doping configurations were explored for potential applications in adsorbing sulfur *** showed that both B or Si single-atom doping and B-Si codoping could substantially enhance the electron transport properties of GR and GDY,improving their surface ***,B and Si atoms displayed synergistic effects for the codoped configurations,where B-Si codoped GR/GDY exhibited much better performance in the adsorption of sulfurcontaining chemicals than single-atom doped *** addition,results demonstrated that,after B-Si codoping,the adsorption energy and charge transfer amounts of GDY with sulfur compounds were much larger than those of GR,indicating that B-Si codoped GDY might be a favorable material for more effectively interacting with sulfur reagents.

关键词： adsorption density functional theory doping graphdiyne graphene sulfur compounds

Polystyrene microplastics disrupted physical barriers, microbiota composition and immune responses in the cecum of developmental Japanese quails

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Journal of Environmental sciences 2024年第10期144卷 225-235页

作者： Lingyang Jing Yuxin Zhang Qingyu Zhang Hongfeng Zhao college of life sciences Shaanxi Normal UniversityXi’an 710119China

Microplastics,a new type of emerging pollutant,is ubiquitous in terrestrial and water *** have become a growing concern due to their impacts on the environment,animal,and human *** also suffer from microplastics *** this study,we examined the toxic effects of polystyrene microplastics(PS-MPs)exposure on physical barrier,microbial community,and immune function in the cecum of a model bird species—Japanese quail(Coturnix japonica).The one-week-old birds were fed on environmentally relevant concentrations of 20μg/kg,400μg/kg,and 8 mg/kg PS-MPs in the diet for 5 *** results showed that microplastics could cause microstructural damages characterized by lamina propria damage and epithelial cell vacuolation and ultrastructural injuries including microvilli breakage and disarrangement aswell asmitochondrial vacuolation in the cecum of *** particular,blurry tight junctions,wider desmosomes spacing,and gene expression alteration indicated cecal tight junction ***,mucous layer breakdown andmucin decrease indicated that chemical barrier was disturbed by ***-MPs also changed cecal microbial *** addition,structural deformation of cecal tonsils and increasing proinflammatory cytokines suggested cecal immune disorder and inflammation responses by PS-MPs *** results suggested that microplastics negatively affected digestive system and might pose great health risks to terrestrial birds.

关键词： Microplastics Gut barrier Histology Inflammation Immunity Oxidative stress

Selective immobilization of Pb(Ⅱ)by biogenic whewellite and its mechanism

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Journal of Environmental sciences 2024年第3期137卷 664-676页

作者： Xingxing Wang Kaiyan Ren Kairui Jiao Wenjun Nie Xiaochi An Bin Lian college of life sciences College of Marine Science and EngineeringNanjing Normal UniversityNanjing 210023China

The development of bio-adsorbents with highly selective immobilization properties for specific heavy metals is a great challenge,but has important application *** whewellite(BW)with high selectivity for Pb(Ⅱ)was synthesized by mineral microbial *** selective immobilization properties and mechanism of BW for Pb(Ⅱ)were analyzed by combining mineral characterization technology and batch adsorption research *** results indicated that BW can efficiently and selectively immobilize Pb(Ⅱ)in single or composite heavy metal adsorption solutions,and the immobilized Pb(Ⅱ)is difficult to *** undergoes monolayer adsorption on Pb(Ⅱ),Qmax≈1073.17 mg/*** immobilization of Pb(Ⅱ)by BW is a physico-chemical adsorption process with spontaneous heat absorption and an accompanying increase in *** addition,the sequestration of Pb(Ⅱ)by BW remains around 756.99 mg/g even at p H=*** excellent selective immobilization properties of BW for Pb(Ⅱ)are closely related to its smaller Ksp,electrostatic repulsion effect,organic-inorganic composite structure,acid resistance and the formation of Pb(Ⅱ)*** study provides beneficial information about the recycling of lead in acidic lead-containing wastewater and composite heavy metal contaminated water bodies.

关键词： Aspergillus niger Biogenic whewellite Pb(Ⅱ) Selective adsorption Immobilization mechanism

Inhibition mechanisms of CRISPR-Cas9 by AcrⅡA25.1 and AcrⅡA32

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Science China(life sciences) 2024年第9期67卷 1781-1791页

作者： Jianlin Zheng Yuwei Zhu Tengjin Huang Wenbo Gao Jiale He Zhiwei Huang HIT Center for life sciences School of Life Science and TechnologyHarbin Institute of TechnologyHarbin 150080China Westlake Center for Genome Editing Westlake Laboratory of Life Sciences and BiomedicineSchool of Life SciencesWestlake UniversityHangzhou 310024China

In the ongoing arms race between bacteria and bacteriophages,bacteriophages have evolved anti-CRISPR proteins to counteract bacterial CRISPR-Cas ***,AcrⅡA25.1 and AcrⅡA32 have been found to effectively inhibit the activity of Spy Cas9 both in bacterial and human ***,their molecular mechanisms remain ***,we report the cryo-electron microscopy structures of ternary complexes formed by AcrⅡA25.1 and AcrⅡA32 bound to Spy Cas9-sg *** structural analysis and biochemical experiments,we revealed that AcrⅡA25.1 and AcrⅡA32 recognize a novel,previously-unidentified anti-CRISPR binding site on Spy *** found that both AcrⅡA25.1 and AcrⅡA32 directly interact with the WED domain,where they spatially obstruct conformational changes of the WED and PI domains,thereby inhibiting Spy Cas9 from recognizing protospacer adjacent motif(PAM)and unwinding double-stranded *** addition,they may inhibit nuclease activity by blocking the dynamic conformational changes of the Spy Cas9 surveillance *** summary,our data elucidate the inhibition mechanisms of two new anti-CRISPR proteins,provide new strategies for the modulation of Spy Cas9 activity,and expand our understanding of the diversity of anti-CRISPR protein inhibition mechanisms.

关键词： anti-CRISPR cryo-EM Spy Cas9 AcrⅡA25.1 AcrⅡA32 inhibiton

TIP60 acetylation of Bub1 regulates centromeric H2AT120 phosphorylation for faithful chromosome segregation

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Science China(life sciences) 2024年第9期67卷 1957-1969页

作者： Mengjie Sun Biying Yang Guangwei Xin Yao Wang Jia Luo Qing Jiang Chuanmao Zhang The Academy for Cell and life Health Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunming 650500China The Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education College of Life SciencesPeking UniversityBeijing 100871China

Proper function of the centromeres ensures correct attachment of kinetochores to spindle microtubules and faithful chromosome segregation in *** in the integrity and function of centromeres can result in chromosome missegregation and genomic ***1 is essential for the mitotic centromere dynamics,yet the underlying molecular mechanisms remain largely ***,we demonstrate that TIP60 acetylates Bub1 at K424 and K431 on kinetochores in early *** acetylation increases the kinase activity of Bub1 to phosphorylate centromeric histone H2A at T120(H2Ap T120),which recruits Aurora B and Shugoshin 1(Sgo1)to regulate centromere integrity,protect centromeric cohesion,and ensure the subsequent faithful chromosome *** of the nonacetylated Bub1 mutant reduces its kinase activity,decreases the level of H2Ap T120,and disrupts the recruitment of centromere proteins and chromosome congression,leading to genomic instability of daughter *** cells exit mitosis,HDAC1-regulated deacetylation of Bub1 decreases H2Ap T120 levels and thereby promotes the departure of centromeric CPC and Sgo1,ensuring timely centromeres ***,our results reveal a molecular mechanism by which the acetylation and deacetylation cycle of Bub1 modulates the phosphorylation of H2A at T120 for recruitment of Aurora B and Sgo1 to the centromeres,ensuring faithful chromosome segregation during mitosis.

关键词： acetylation Bub1 TIP60 H2ApT120 chromosome segregation

Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode,Bursaphelenchus xylophilus

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Journal of Forestry Research 2024年第5期35卷 191-200页

作者： Shuisong Liu Linsong Wang Ronggui Li Mengyu Chen Wenjun Deng Chao Wang Guicai Du Qunqun Guo college of life sciences Qingdao UniversityQingdao 266071People’s Republic of China

In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be *** situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive *** in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite *** addition,RNAi of sft-4 changed the female–male ratio and lifespan of *** bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly *** results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.

关键词： Black pine Pinaceae Bursaphelenchus xylophilus Sft-4 In situ hybridization RNAi Pathogenicity

Strigolactones modulate cotton fiber elongation and secondary cell wall thickening

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Journal of Integrative Agriculture 2024年第6期23卷 1850-1863页

作者： Yunze Wen Peng He Xiaohan Bai Huizhi Zhang Yunfeng Zhang Jianing Yu college of life sciences Shaanxi Normal UniversityXi’an 710119China

Cotton is one of the most important economic crops in the world,and it is a major source of fiber in the textile ***(SLs)are a class of carotenoid-derived plant hormones involved in many processes of plant growth and development,although the functions of SL in fiber development remain largely ***,we found that the endogenous SLs were significantly higher in fibers at 20 days post-anthesis(DPA).Exogenous SLs significantly increased fiber length and cell wall ***,we cloned three key SL biosynthetic genes,namely GhD27,GhMAX3,and GhMAX4,which were highly expressed in fibers,and subcellular localization analyses revealed that GhD27,GhMAX3,and GhMAX4 were localized in the *** exogenous expression of GhD27,GhMAX3,and GhMAX4 complemented the physiological phenotypes of d27,max3,and max4 mutations in Arabidopsis,*** of GhD27,GhMAX3,and GhMAX4 in cotton resulted in increased numbers of axillary buds and leaves,reduced fiber length,and significantly reduced fiber *** findings revealed that SLs participate in plant growth,fiber elongation,and secondary cell wall formation in *** results provide new and effective genetic resources for improving cotton fiber yield and plant architecture.

关键词： strigolactones fiber elongation secondary cell wall thickening cotton

Endochondral ossification of hindlimbs in embryonic development of Japanese Quail(Coturnix japonica)

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Avian Research 2024年第1期15卷 123-132页

作者： Xuan Li Yuxin Zhang Hongfeng Zhao college of life sciences Shaanxi Normal UniversityXi’an710119China

The endochondral ossification of hindlimb is essential to a bird’s ability to stand,walk and *** hindlimb is ossified in the embryos before hatching in precocial ***,the molecular mechanisms of hindlimb ossification in birds is still ***,we tried to examine the process of hindlimb ossification and its molecular regulation by using an animal model—Japanese Quail(Coturnix japonica).We selected four critical stages(Embryo Day:E6,E8,E12 and E16) of skeletal development of embryonic quails for hindlimb skeleton staining to show the process of endochondral ossification and to examine the molecular regulation of endochondral osteogenesis by RNA-Seq *** results showed that ossification became increased with embryonic development and most hindlimb was ossified before ***-Seq analysis revealed that various signaling pathways were involved with endochondral ossification with thyroid hormone signaling and WNT signaling pathway particularly ***,the expression levels of 42 genes were continuously upregulated and 14 genes were continuously downregulated from E6 to *** present study might provide new insights into complex molecular mechanisms in regulation of endochondral ossification.

关键词： Embryos Hindlimb Ossification Precocial Transcriptomics

GhCTSF1,a short PPR protein with a conserved role in chloroplast development and photosynthesis,participates in intron splicing of rpoC1 and ycf3-2 transcripts in cotton

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Plant Communications 2024年第6期5卷 213-229页

作者： Yuzhu Huo Mengxue Cheng Meiju Tang Meng Zhang Xiaofan Yang Yating Zheng Tong Zhao Peng He Jianing Yu college of life sciences Shaanxi Normal UniversityXi’an 710119China

Cotton is one of the most important textile fibers *** crucial agronomic traits,leaves play an essential role in the growth,disease resistance,fiber quality,and yield of cotton *** repeat(PPR)proteins are a large family of nuclear-encoded proteins involved in organellar or nuclear RNA *** a virus-induced gene silencing assay,we found that cotton plants displayed variegated yellow leaf phenotypes with decreased chlorophyll content when expression of the PPR gene GhCTSF1 was ***1 encodes a chloroplast-localized protein that contains only two PPR *** of GhCTSF1 substantially reduces the splicing efficiency of rpoC1 intron 1 and ycf3 intron *** of function of the GhCTSF1 ortholog EMB1417 causes splicing defects in rpoC1 and ycf3-2,leading to impaired chloroplast structure and decreased photosynthetic rates in *** also found that GhCTSF1 interacts with two splicing factors,GhCRS2 and *** in GhCRS2 and GhWTF1 severely affect intron splicing of rpoC1 and ycf3-2 in cotton,leading to defects in chloroplast development and a reduction in *** results suggest that GhCTSF1 is specifically required for splicing rpoC1 and ycf3-2 in cooperation with GhCRS2 and GhWTF1.

关键词： chloroplast RNA splicing PPR protein cotton photosynthesis