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Assessment of Spermatic Chromatin Decondensation by the Toluidine Blue Assay in Infertile Patients in Cotonou

Advances in Reproductive Sciences 2022年第2期10卷 37-48页

作者： Yaude Alfred Azonbakin Simon Aweh Bruno Dangbemey Patrice Sissoko Sidi Boula Dossou Jean-Paul Flore Gangbo Laleye Anatole Laboratory of Histology Reproductive Biology Cytogenetics and Medical Genetics Faculty of Health Sciences Cotonou Benin Department of Gynaecology-Obstetrics Mother and Child Teaching Hospital of Cotonou Cotonou Benin Pathological Anatomy and Cytology Department of the point’s G Teaching Hospital in Bamako Mali Laboratory of Andrology Human Reproduction and Demography Research Center CERRHUD Cotonou Benin

Background: A high level of sperm chromatin decondensation results in loss of DNA quality and it is associated with poor embryonic prognosis in Assisted Reproductive Technology (ART). This had lead to introduction of sperm DNA compaction testing in assessment of clinical male infertility. Toluidine blue assay is one of most commonly used tests to measure chromatin decondensation in research studies and also in clinical routine use. So the objective of our study was to assess the level of sperm chromatin decondensation by toluidine blue assay in men dealing with couple infertility. Methods: The sperm samples of 96 patients referred for infertility, over a 4-month period from September 2020 to January 2021, were stained with toluidine blue and observed under an optical microscope. These were evaluated according to the World Health Organization. Results: The 25 - 34 age group was the most represented among infertile men. There was a non-significant correlation between age and the level of spermatic chromatin condensation (r = 0.0348). Spermatic parameters had averages above standards norms, apart from that of low vitality. Spermatic chromatin decondenization affected 96% of infertile patients. Asthenozoospermia was the most common abnormality. Among standard parameters of spermogram, only the concentration of spermatozoa in the semen was weakly correlated (r = 0.18) with sperm chromatin decondensation. Conclusion: The level of sperm chromatin condensation is, on the one hand, a parameter independent of the age of the subject; on the other hand, allows to evaluate male infertility, like the classic parameters of the spermogram and spermocytogram. However, in view of the high probability of changes in the nuclear quality of sperm intrinsic to the analysis process and sperm concentration, an improvement of the toluidine blue test using the swim-up technique and associated with image processing software is possible.

关键词： Male Infertility Decondensation Spermatic Chromatin Toluidine Blue

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analysis on the Supply and Demand Situation of Global Ge Mineral Resources

Analysis on the Supply and Demand Situation of Global Ge Min...

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作者： Chao LIU Ting ZHAO Xiaoyuan ZHANG Fei LIANG Institute of Mineral Resources Chinese Academy of Geological Sciences Research center for Global Mineral Resources Chinese Academy of Geological Sciences MNR Key Laboratory of Metallogeny and Mineral Assessment Institute of Mineral ResourcesCAGS School of Earth Sciences and Resources China University of Geosciences(Beijing)

As a high-tech material,germanium is widely used in the fields of optical fiber communication,national defense science and technology,aerospace technology,medical care,geological exploration,chemical catalysis and semiconductor *** is a big country in the production and consumption of germanium resources,but it is not a strong country in germanium *** with resources,this paper mainly analyzed and studied the current situation of global germanium supply and demand,analyzed the future trend of germanium consumption,and put forward some suggestions to improve the development of germanium resources industry in China.

关键词： germanium mineral supply and demand future trend

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Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium

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Cell Regeneration 2022年第1期11卷 172-187页

作者： Haonan Li Xiaodan Wang Yalong Wang Mengxian Zhang Fan Hong Hong Wang Along Cui Jianguo Zhao Weizhi Ji Ye-Guang Chen The State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou Science ParkGuangzhou 510530China Guangzhou Laboratory Guangzhou 510005China State Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunming 650500YunnanChina State Key Laboratory of Stem cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

Animal models are widely used for biomedical studies and drug *** small intestine plays key roles in nutrient absorption,hormone secretion,microbiota defense and drug absorption and *** the intestinal structure of mammals is conserved,the differences on epithelial cell composition,functional assignments and drug absorption among mammals are largely ***,cross-species analysis of single-cell transcriptomic atlas of the ileum epithelium from mouse,rat,pig,macaque and human reveals the conserved and differential cell types and functions among species,identifies a new CA7+cell type in pig,macaque and human ileum,uncovers the distinct expression pattern in enterocytes,enteroendocrine cells and Paneth cells,and defines the conserved and species-specific intestinal stem cell signature *** examination of drug absorption across species suggests that drug metabolism in mouse ileum is closer to human while drug transport in macaque ileum is more similar to ***,our data provide the comprehensive information about cell composition and functional assignments in five species,and offer the valuable guidance for animal model selection and drug testing.

关键词： scRNA-seq Transcriptome Ileum Monkey Pig Rat CA7 cells

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Qualitative and Quantitative analysis of Linoleic Acid in Polygonati Rhizoma

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Digital Chinese Medicine 2020年第3期3卷 180-187页

作者： ZHOU Zhun YUAN Han-Wen PENG Cai-Yun JIAN Yu-Qing ZHOU Xu-Dong LI Bin SHENG Wen-Bing GONG Li-Min HE Shu-Jin WANG Wei LIU Chang-Xiao Sino-Pakistan TCM and Ethnomedicine Research center Innovative Materia Medica Research InstituteSchool of PharmacyHunan University of Chinese MedicineChangshaHunan 410208China Hunan Xinhui Pharmaceutical Co. Ltd.ChangshaHunan 410000China Tianjin Institute of Pharmaceutical Research Tianjin 300193China

Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality *** The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid *** chromatogram was improved significantly after optimization for qualitative *** The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest *** study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).

关键词： Polygonati Rhizoma(Huang Jing,黄精) Linoleic acid Qualitative analysis Quantitative analysis Thin layer chromatography(TLC) High performance liquid chromatography(HPLC)

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Mechanical characterization of rose bengal and green light crosslinked collagen scaffolds for regenerative medicine

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Regenerative Biomaterials 2021年第6期8卷 185-198页

作者： Joy Braun Stefanie Eckes Michelle Fiona Kilb Dirk Fischer Claudia Eßbach Pol Maria Rommens Philipp Drees Katja Schmitz Daniela Nickel Ulrike Ritz Department of Orthopedics and Traumatology BiomaTiCSUniversity Medical CenterJohannes Gutenberg UniversityLangenbeckstraße 1Mainz 55131Germany Clemens-Schopf-Institute of Organic Chemistry and Biochemistry Technical University of DarmstadtAlarich-Weiss-Straße 4Darmstadt 64287Germany Berufsakademie Sachsen—Staatliche Studienakademie Glauchau University of Cooperative EducationKopernikusstraße 51Glauchau 08371Germany

Collagen is one of the most important biomaterials for tissue engineering *** its excellent biocompatibility,it shows the non-negligible disadvantage of poor mechanical *** crosslinking with rose bengal and green light(RGX)is an appropriate method to improve this *** development of collagen laminates is helpful for further adjustment of the mechanical properties as well as the controlled release of incorporated *** this study,we investigate the impact of crosslinking and layering of two different collagen scaffolds on the swelling behavior andmechanical behavior inmicro tensile tests to obtain information on its wearing comfort(stiffness,strength and ductility).The mechanical stability of the collagen material after degradation due to cell contact is examined using thickness *** is no linear increase or decrease due to layering homologous ***,a decrease in elongation at break,Young’s modulus and ultimate tensile strength are measured when the untreated monolayer is compared to the crosslinked ***,we can detect a connection between stability and cell *** results show that with variation in number and type of layers,collagen scaffolds with tailored mechanical properties can be *** a multi-layered structure enables the release of biomolecules into inner or outer layers for biomedical applications.

关键词： collagen type I collagen laminates rose bengal and green light crosslinking thickness analysis micro tensile testing cell-collagen interactions

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Discussion and Research on Orthopedic Biomechanics testing Technology

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Journal of Clinical and Nursing Research 2020年第6期4卷 67-70页

作者： Jinkui Liang Wanzong Liu Xiuxiu Wang Yuanyu Li Shandong Quality Inspection center for Medical Devices Jinan 250101Shandong ProvinceChina

With the continuous development of today's science and technology,orthopedic research has also achieved continuous updates in materials and *** this case,the mechanics testing technology of orthopedics also needs to be further updated and developed,so that it can effectively meet the requirement for today's orthopedic mechanical *** on this,this article analyzes several advanced orthopedic mechanics testing *** is hoped that this analysis can provide a reference for the good application and development of orthopedic mechanics testing technology.

关键词： Orthopedics Biomechanics Mechanical testing,testing technology

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Modeling of FAME, MG, TG and DG in Rapeseed Oil Biodiesel by Near Infrared Spectroscopy

Modeling of FAME, MG, TG and DG in Rapeseed Oil Biodiesel by...

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第十二届中国酶工程学术研讨会

作者： Minyuan Tang Huabiao Miao Qian Wu Nanyu Han Yuelin Mu Junpei Zhou Junmei Ding Yunjuan Yang Yueyue Feng Zunxi Huang School of Life Sciences Yunnan Normal University Engineering Research center of Sustainable and Utilization of Biomass Energy Ministry of Education Yunnan Normal University Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment Yunnan Normal University Key Laboratory of Enzyme Engineering Yunnan Normal University

Near infrared spectroscopy is befitting the determination of the biodiesel components which is compared with GC, HPLC and SEC. Our work focuses on the development of the NIR model based on NIRs and GC-MS to determine FAME, MG, DG, TG in biodiesel which is produced from rapeseed oil by bio-enzyme catalysis. The result shows that the mathematical pretreatments selected respectively 1, 4, 4, 1 and the scatter correction was SNV + Detrend, the near infrared model of rapeseed oil biodiesel can be developed by partial least squares（PLS）. The model can be used to rapidly determine the main components of rapeseed oil biodiesel. In this work rapeseed oil was used as raw material oil to prepare biodiesel, so the model was applied to the determination of rapeseed oil biodiesel. The parameters of the near infrared models showed that the prediction results of the model are more accurate and have better prediction ability. Compared with other methods, the NIRs have the advantages of simple operation, fast analysis and affordable price. This work used rapeseed oil as raw material oil to prepare biodiesel, so the model was applied to the determination of rapeseed oil biodiesel. If we want to measure other biodiesel, we need to add a certain amount of biodiesel with other oils to make the sample more representatives and further improve the model.

关键词： Biodiesel FAME GC-MS NIR Rapeseed Oil

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Spatiotemporal Variability and Change of the South China Spring Precipitation during 1961-2012

Spatiotemporal Variability and Change of the South China Spr...

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第34届中国气象学会年会

作者： Hong-Lan Liu Qiang Zhang Jun-Qin Guo Jun-Guo Zhang and Sheng Wang Zhangye Meteorological Bureau Institute of Arid Meteorology of China Meteorological Administration/Key Laboratory of Arid Climatic Change and Disaster Reduction of Gansu Province Key Laboratory of Arid Climate Change and Disaster Reduction of China Meteorological Administration Northwest Regional Climate center Zhangye Middle school

In this study, we analyze precipitation data from 47 meteorological stations spanning between 1961 and 2012 and NCEP/NCAR reanalysis to understand spatiotemporal variability and change of spring precipitation of South China and their relations to atmospheric circulations. Empirical Orthogonal Function（EOF） analysis and Rotated EOF（REOF） are used to reveal dominant spatial structures of precipitation anomaly and MannKendall testing method to determine the temporal locations of abrupt changes during the analyzed time span. We find that the first spatial mode of the spring precipitation ofthe South China has a domain uniform structure;the second is dominated by a spatial dipole;and the third contains six variability centers. 1980 s was the decade of the largest amount of precipitation while 1960 s the decade of the smallest amount of precipitation. The spring precipitation also appeared to have a decreasing trend since 2000. We also find that spring precipitation of the South China have experienced a few abrupt changes: sudden increment at 1964, sudden decrement at 2002, and sudden increment at 1995. In addition to these abrupt changes, the precipitation could also be characterized by variability of multiple temporal scales, with dominant periodicities of 4-year, 8-year, and 14-year. The South China spring precipitation is also closely tied to the atmospheric circulations: when Aleutian Low strengthens, westerly weakens, and the center of the Western Pacific subtropical high shifts southeastward in the early spring;and the South China precipitation tends to be abundant（positive anomaly）. In contrast, when Ural ridge strengthens, the southern branch of the East Asian trough weakens and the Western Pacific subtropical high shifts northwestward in the early spring, the South China precipitation tends to be reduced（negative anomaly）.

关键词： spring precipitation spatiotemporal structure atmospheric circulation South China region

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A conductive supramolecular hydrogel creates ideal endogenous niches to promote spinal cord injury repair

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Bioactive materials 2022年第9期7卷 103-119页

作者： Biao Yang Chengzhen Liang Di Chen Feng Cheng Yuang Zhang Shaoke Wang Jiawei Shu Xianpeng Huang Jingkai Wang Kaishun Xia Liwei Ying Kesi Shi Chenggui Wang Xuhua Wang Fangcai Li Qian Zhao Qixin Chen Department of Orthopedics Surgery The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou310009ZhejiangChina Orthopedics Research Institute of Zhejiang University Hangzhou310009ZhejiangChina Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province Hangzhou310009ZhejiangChina Ningbo Research Institute of Zhejiang University Ningbo315100ZhejiangChina State Key Laboratory of Chemical Engineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou310027ZhejiangChina NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Research and Brain-Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhou310003ZhejiangChina

The current effective method for treatment of spinal cord injury(SCI)is to reconstruct the biological microenvironment by filling the injured cavity area and increasing neuronal differentiation of neural stem cells(NSCs)to repair ***,the method is characterized by several challenges including irregular wounds,and mechanical and electrical mismatch of the material-tissue *** the current study,a unique and facile agarose/gelatin/polypyrrole(Aga/Gel/PPy,AGP3)hydrogel with similar conductivity and modulus as the spinal cord was developed by altering the concentration of Aga and *** gelation occurred through non-covalent interactions,and the physically crosslinked features made the AGP3 hydrogels *** vitro cultures showed that AGP3 hydrogel exhibited excellent biocompatibility,and promoted differentiation of NSCs toward neurons whereas it inhibited over-proliferation of *** in vivo implanted AGP3 hydrogel completely covered the tissue defects and reduced injured cavity *** vivo studies further showed that the AGP3 hydrogel provided a biocompatible microenvironment for promoting endogenous neurogenesis rather than glial fibrosis formation,resulting in significant functional *** sequencing analysis further indicated that AGP3 hydrogel significantly modulated expression of neurogenesis-related genes through intracellular Ca2+signaling ***,this supramolecular strategy produces AGP3 hydrogel that can be used as favorable biomaterials for SCI repair by filling the cavity and imitating the physiological properties of the spinal cord.

关键词： Conducting polymer Supramolecular hydrogels Biomimetic scaffolds Nerve regeneration Spinal cord injury

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CURRENT AND FUTURE APPROACHES TO THE QUALITY OF CONTROL OF CHINESE HERBAL MEDICINES

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World Journal of Traditional Chinese Medicine 2015年第4期1卷 65-65页

作者： Rudolf Bauer TCM Research center Graz Institute of Pharmaceutical Sciences Karl-Franzens-University Graz

Quality of control of Chinese herbal medicines is currently mainly based on specifications of *** are the limited specificiity of some of these methods,the use of various grades and processed materials,which cannot easily be distinguished,and the increasing use of granules,which are so far not considered in most *** identity and purity testing,microscopy and TLC fingerprint analysis are

关键词： currently distinguished medicines granules processed herbal fingerprint specifications purity pesticides

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