检索结果-南通市图书馆

Regulatory effects of dietary L-Arg supplementation on the innate immunity and antioxidant ability in broiler chickens

引用

Journal of Integrative Agriculture 2016年第11期15卷 2578-2587页

作者： HU Ya-di TAN Jian-zhuang QI Ji ZHANG Hong-fu state key laboratory of animal nutrition Institute of Animal SciencesChinese Academy of Agricultural Sciences

Here, we investigated the effect of dietary arginine（Arg） supplementation on innate immunity and the antioxidant ability of broiler chickens. The experiment was designed as a single-factorial arrangement（n=8 cages/treatment, six birds/cage）, and we used four dietary Arg concentrations（10.0, 15.0, 20.0 or 25.0 g kg–1）. On day 21, the birds were killed to obtain spleen, cecal tonsil and liver samples to determine the gene expression and antioxidant characteristics. Increasing the Arg concentration linearly decreased（P0.05） the m RNA expression of splenic interleukin-18（IL-18） and tumor necrosis factor-α（TNF-α）. Dietary Arg supplementation quadratically decreased（P0.05） the expression of interleukin-1b（IL-1b） and interferon-γ（IFN-γ） m RNA in the spleen. Increasing Arg concentrations linearly and quadratically reduced the expression of IL-18 m RNA in the spleen. Meanwhile, increasing dietary Arg supplementation linearly and quadratically increased the lymphotactin m RNA（P0.05） expression, and linearly increased the macrophage inflammatory protein-1β（MIP-1β） and toll-like receptor 15（TLR15） m RNA expression in the cecal tonsils. Dietary Arg supplementation linearly（P0.05） increased the glutathione peroxidase（GSH-Px）, catalase（CAT）, and lysozyme（LZM） activities in the liver. However, the malondialdehyde（MDA） activity in the liver was not influenced by the dietary Arg concentration（P0.05）. No significant（P0.05） effect was found on the activity of superoxide dismutase（SOD） in the liver. Thus high levels of Arg supplementation（20.0 g kg^（–1）） may potentially suppress the innate immunity of broiler chickens, and dietary Arg supplementation enhances the antioxidant activity in broiler chickens.

关键词： arginine innate immunity antioxidant ability broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chicken gga-miR-1306-5p targets Tollip and plays an important role in host response against Salmonella enteritidis infection

引用

Journal of animal Science and Biotechnology 2019年第4期10卷 873-883页

作者： Weiwei Sun Ranran Liu Peng Li Qinghe Li Huanxian Cui Maiqing Zheng Jie Wen Guiping Zhao institute of animal sciences Chinese Academy of Agricultural Sciences State Key Laboratory of Animal Nutrition

Background: Increasing evidence indicates that micro RNAs(mi RNAs) are involved in inflammatory response and immune regulation following pathogen invasion. The purpose of this study was to elucidate the roles played by Gallus gallus micro RNA-1306-5 p(gga-mi R-1306-5 p) in host responses against potential invasion by Salmonella enteritidis(SE) in chickens and the underlying ***: In present study, the expression levels of gga-mi R-1306-5 p were determined in both tissues and HD11 cells. The results showed that gga-mi R-1306-5 p was significantly increased following SE infection or lipopolysaccharide(LPS) stimulation. The dual luciferase reporter assay further validated that gga-mi R-1306-5 p targeted the Toll-interacting protein(Tollip), and thereby participated in the regulation of immune response against SE or LPS stimulation through binding with the 3′-untranslated region(3’UTR) of Tollip. Additionally, the expression of Tollip was significantly blocked by over-expressed gga-mi R-1306-5 p. The underlying mechanisms by which ggami R-1306-5 p modulated the production of pro-inflammatory cytokines were also investigated. Molecular biological assays demonstrated that overexpression of gga-mi R-1306-5 p promoted the production of pro-inflammatory mediators, including NF-κB, TNF-α, IL-6, and IL-1β, which produced effects similar to those of Tollip ***: Taken together, gga-mi R-1306-5 p induced by SE or LPS, regulates the immune response by inhibiting Tollip, which activates the production of inflammatory cytokines. This study has provided the first direct evidence that gga-mi R-1306-5 p targets Tollip, and is involved in the host response against SE.

关键词： gga-miR-1306-5p LPS Pro-inflammatory cytokine Salmonella enteritidis Tollip

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Graded Levels of Fiber from Alfalfa Meal on Nutrient Digestibility and Flow of Fattening Pigs

引用

Journal of Integrative Agriculture 2014年第8期13卷 1746-1752页

作者： CHEN Liang GAO Li-xiang ZHANG Hong-fu state key laboratory of animal nutrition Institute of Animal Sciences Chinese Academy of Agricultural Sciences

The fiber level and composition have an important effect on nutrient digestibility of swine diets. Little information is known about the effects of fiber level and composition from alfalfa meal on nutrient digestibility of fattening pigs fed a corn-soybean meal-based diet. The objective of this experiment was to determine the effects of alfalfa fiber on the growth performance, intestinal nutrient flow and apparent total tract digestibility（ATTD） of nutrients in fattening pigs. 24 barrows（Duroc×（Large White×Landrace）, body weight=（60.6±0.7） kg） were randomly allotted to 4 treatments with 6 replicates of 1 pig per replicate. The pigs were provided a control diet or a diet containing 5, 10 or 20% of alfalfa meal during a 14-d experiment period. Average daily gain（ADG） and the ATTD of dry matter（DM）, organic matter（OM）, crude protein（CP）, neutral detergent fiber（NDF）, acid detergent fiber（ADF） and gross energy（GE） reduced linearly as the level of alfalfa meal in the diet increased（P〈0.01）. The total tract flow of DM, OM, CP, NDF, ADF, and GE increased with the increase in dietary alfalfa（linear, P〈0.05）. Growth performance and nutrient digestion were not affected by inclusion of 5% alfalfa meal in the diet（P〉0.05）. A multiple linear regression analysis, taking into account both soluble and insoluble fiber intake, explained approximately 70% of the variation in the ATTD of DM, OM, NDF, and GE（P〈0.01）. In conclusion, alfalfa meal should be limited to less than 5% of the diet in fattening pigs to maximize growth performance and nutrient digestion. Soluble and insoluble fiber from alfalfa meal has the differential roles in nutrient digestion, which may help explain the main variation observed in nutrient digestibility. These findings suggest that knowledge of specific fiber components is necessary to accurately predict the effects of dietary fiber on nutrient digestibility.

关键词： alfalfa digestibility fiber flow nutrient pig

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of sustained cold and heat stress on energy intake, growth and mitochondrial function of broiler chickens

引用

Journal of Integrative Agriculture 2016年第10期15卷 2336-2342页

作者： ZHANG Shao-shuai SU Hong-guang ZHOU Ying LI Xiu-mei FENG Jing-hai ZHANG Min-hong state key laboratory of animal nutrition Institute of Animal Sciences Chinese Academy of Agricultural Sciences

To study the correlation of broiler chickens with energy intake, growth and mitochondrial function which exposed to sustained cold and heat stress and to find out the comfortable temperature, 288 broiler chickens（21-day with（748±26） g, 144 males and 144 females） were divided randomly into six temperature-controlled chambers. Each chamber contained six cages including eight AA broilers per cage, each cage as a repeat. After acclimation for one week（temperature, 21℃; relative humidity, 60%）, the temperature of each chamber was adjusted（finished within 1 h） respectively to 10, 14, 18, 22, 26, or 30℃（RH, 60%） for a 14-day experimental period. After treatment, gross energy intake（GEI）, metabolizable energy intake（MEI）, the ratio of MEI/BW, metabolizability, average daily gain（ADG）, the concentration of liver mitochondria protein and cytochrome c oxidase（CCO） were measured respectively. Our results confirmed that when the temperature over 26℃ for 14 days, GEI, MEI and CCO activities were decreased significantly（P〈0.05）, but the concentration of liver mitochondria protein was increased and metabolizability of broilers was not influenced（P〉0.05）. Compared with treatment for 14 days, the ratio of MEI/body weight（BW） were also decreased when the temperature over 26℃ after temperature stress for 7 days（P〈0.05）, meanwhile mitochondrial protein concentration was increased at 10℃ and CCO activity was not affected（P〉0.05）. Additionally at 22℃, the ADG reached the maximal value. When kept in uncomfortable temperatures for a long time, the ADG and CCO activities of broiler were reduced, which was accompanied by mitochondrial hyperplasia. In summary, our study focused on the performance of broilers during sustained cold and heat environmental temperatures ranging from 10 to 30℃. From the point of view of energy utilization, moreover, 22 to 26℃ is comfortable for 28–42 day s broilers. And these could provide the theoretical basis on the high efficient production.

关键词： broiler chicken cytochrome c oxidase energy intake metabolizability sustained cold and heat stress

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS

引用

Science China(Life sciences) 2016年第11期59卷 1166-1176页

作者： Yan Xiong Xiangfang Tang Qingshi Meng Hongfu Zhang state key laboratory of animal nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences

Ammonia has been considered the contaminant primarily responsible for respiratory disease in poultry. Even though it can cause tracheal lesions, its adverse effects on the trachea have not been sufficiently studied. The present study investigated tracheal changes in Arbor Acres broilers(Gallus gallus) induced by high concentration of ammonia using isobaric tag for relative and absolute quantification(iTRAQ)-based proteome analysis. In total, 3,706 proteins within false discovery rate of 1% were identified, including 119 significantly differentially expressed proteins. Functional analysis revealed that proteins related to immune response and muscle contraction were significantly enriched. With respect to the immune response, up-regulated proteins(like FGA) were pro-inflammatory, while down-regulated proteins participated in antigen processing and antigen presenting(like MYO1G), immunoglobulin and cathelicidin production(like fowlicidin-2), and immunodeficiency(like PTPRC). Regarding muscle contraction, all differentially expressed proteins(like TPM1) were up-regulated. An over-expression of mucin, which is a common feature of airway disease, was also observed. Additionally, the transcriptional alterations of 6 selected proteins were analyzed by quantitative RT-PCR. Overall, proteomic changes suggested the onset of airway obstruction and diminished host defense in trachea after ammonia exposure. These results may serve as a valuable reference for future interventions against ammonia toxicity.

关键词： ammonia broiler proteomics trachea

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of relative humidity at chronic temperature on growth performance, glucose consumption, and mitochondrial ATP production of broilers

引用

Journal of Integrative Agriculture 2019年第6期18卷 1321-1328页

作者： ZHOU ying ZHANG Min-hong FENG Jing-hai DIAO Hua-jie state key laboratory of animal nutrition Institute of Animal ScienceChinese Academy of Agricultural Sciences

An experiment was conducted to investigate the effect of relative humidity(RH)at chronic temperature on growth performance,glucose consumption,and mitochondrial adenosine triphosphate(ATP)production of broilers.A total of 180 28-day-old Arbor Acres broilers(half males and half females)were randomly allocated to three treatments,each containing six replicates of 10 birds per treatment,using a completely randomized *** were reared at 35,60 or 85%RH at 32℃for 15 days(temperature increased by 3℃every 3 days from 20 to 32℃within 15 days:20–23–26–29–32℃).RH affected(P<0.05)average daily feed intake(ADFI),average daily gain(ADG),average daily water consumption(ADWC),blood glucose concentrations,muscle glycogen levels,avian uncoupling protein(av UCP)mRNA expression,and cytochrome c oxidase(CCO)activity in liver of broilers at 42 days of *** 85%RH decreased(P<0.05)ADFI,ADG and ADWC;35%RH decreased(P<0.02)*** 85 and 35%RH increased(P<0.01)blood glucose and decreased(P<0.05)muscle *** 85 and 35%RH increased(P<0.05)av UCP mRNA expression.35%RH decreased(P<0.05)CCO *** conclusion,both high and low RH inceased glucose consumption and reduced mitochondrial ATP poduction,leading to a decline in growth rate.

关键词： broiler growth performance mitochondrial ATP poduction relative humidity chronic temperature

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of relative humidity at either acute or chronic moderate temperature on growth performance and droppings' corticosterone metabolites of broilers

引用

Journal of Integrative Agriculture 2019年第1期18卷 152-159页

作者： ZHOU ying LI Xiu-mei ZHANG Min-hong FENG Jing-hai state key laboratory of animal nutrition institute of Animal ScienceChinese Academy of Agricultural Sciences

The present study aimed to investigate the effect of relative humidity(RH) at either acute or chronic moderate ambient temperature(Ta) on growth performance and droppings' corticosterone metabolites of *** experiments were conducted: effect of RH(35,60 or 85%) on average daily feed intake(ADFI) and droppings' corticosterone metabolites at acute(1 d: 20–26 or 31–20°C,26 or 31°C for 6 h d–1 at 10:00–16:00) moderate Ta(experiment 1) and effect of RH(35,60 or 85%) on growth performance and droppings' corticosterone metabolites at chronic(step-wisely increasing temperature by 3°C every 3 d from 20 to 32°C within 15 d: 20–23–26–29–32°C) moderate Ta(experiment 2).Droppings were collected at the 2,4,6,8,and 22 h after Ta-RH controlled in experiment 1 and at the 2,4,6,and 22 h after Ta controlled to 32°C in experiment *** results showed that: 1) In experiment 1,85% RH increased(P0.05) was found in ADFI among the three RH groups at acute moderate 26°C; 35 and 85% RH increased(P0.05) in droppings' corticosterone metabolites was found between the 35 and 60% RH,however,35% RH decreased(P<0.01) ADG compared to the 60% RH,and the average of droppings' c

关键词： broiler droppings’ corticosterone metabolites relative humidity acute moderate temperature chronic moderate temperature

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of biochanin A on lactational performance,nitrogen metabolism,and blood metabolites in dairy cows

引用

animal nutrition 2024年第3期18卷 441-449页

作者： Zhanbo Xiong Yanjun Li Xiaoyin Zhang Shiqi Zhang Kexin Li Nan Zheng Shengguo Zhao Jiaqi Wang state key laboratory of animal nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

Optimizing nitrogen utilization efficiency and mitigating nitrogen losses in cows plays a pivotal role in fostering economic sustainability within contemporary agricultural *** A(BCA),a natural component in red clover,has the potential to improve nitrogen metabolism in dairy *** primary objective of this study was to probe the impact of biochanin A supplementation on lactational performance,nitrogen metabolism,and blood metabolites in dairy cows.A complete randomized block design experiment was conducted over 28 d,involving 36 multiparous Holstein cows(comparable milk yield=37.1±2.90 kg,BW=642±70.0 kg,days in milk=92±8.0 d,and parity=2.4±0.50),which were allocated to three treatment groups:the Control group(with 0 g/d BCA),the Low group(with 10 g/d per cow BCA),and the High group(with 40 g/d per cow BCA).Biochanin A supplementation improved the lactational performance of cows by increasing milk yield by 6.3%(P=0.007)and feed efficiency by 12.7%(P=0.009).Total intestinal apparent digestibility was unaffected by BCA supplementation(P>0.05),but microbial nitrogen was increased by 30.0%(P=0.002)for promoting nitrogen utilization efficiency by 20.7%(P=0.004).Milk competent yields(protein,lactose,and non-fat milk solid)were increased with increasing BCA supplementation(P 0.05).BCA did not affect body health of dairy ***,none of the plasma endocrine hormones were affected(P>0.05).A total of 95 significantly different metabolites were screened from the plasma metabolites of cows in the BCA-added and non-added *** performing an enrichment analysis of the metabolic pathways associated with the different metabolites,six specific pathways were identified:bile acid biosynthesis,aspartate metabolism,pyrimidine metabolism,arginine and proline metabolism,the urea cycle,and ammonia *** inclusion of BCA is suggested to enhance milk yield and modulate nitrogen metabolism by influencing relevant metabolites within the metabolic pathwa

关键词： Biochanin A Lactational performance nutritional metabolism Blood metabolite

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of red clover isoflavones on hormone,immune,inflammatory,and plasma biochemistry in lactating dairy cows

引用

animal nutrition 2024年第1期16卷 306-312页

作者： Shiqi Zhang Xiaoyin Zhang Zhanbo Xiong Kexin Li Yuan Gao Ying Bu Nan Zheng Shengguo Zhao Jiaqi Wang state key laboratory of animal nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

This study was to conducted to investigate the effect of red clover isoflavones on the health indicated by immune status and blood biochemistry in dairy ***-eight healthy Holstein lactating cows were randomly divided into four treatments(n=17 per treatment)from 5 blocks according to milk yield using a randomized complete block *** initial differences in parity(2.13±1.21),days in milk(165±21 d),and milk yield(33.93±3.81 kg/d)between *** were fed the basal diet supplemented with 0,2,4,or 8 g/kg red clover extract(RCE)in diet(dry matter based).Feeding,refusal feed weights,and milk yield were recorded three consecutive days in weeks 0,4,8,and *** was collected from the tail vein of the cows on the last day of weeks 4,8 and 12,1 h after the morning feeding,and analyzed for hormones,immunoglobulins,inflammatory markers,and markers of liver and kidney *** dry matter intake was significantly decreased by 3.7%in the 8 g/kg group(P0.05).These results recommend the supplementation of RCE at a level from 2 to4 g/kg DM.

关键词： Dairy cow Red clover isoflavone Immunity Anti-inflammatory

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site

引用

Journal of Integrative Agriculture 2016年第11期15卷 2571-2577页

作者： QIAN Li-li MA De-zun GAO Peng-fei JIANG Sheng-wang WANG Qing-qing CAI Chun-bo XIAO Gao-jun AN Xiao-rong CUI Wen-tao state key laboratory of Agrobiotechnology China Agricultural University state key laboratory for animal nutrition Institute of Animal ScienceChinese Academy of Agricultural Sciences

Myostatin, a member of the transforming growth factor beta（TGF-β） superfamily, is a dominant inhibitor that acts to limit skeletal muscle growth and development. In this study, we generated transgenic mice that express porcine myostatin containg mutations at its cleavage site（RSRR） to evaluate its effect on muscle mass. Results showed that the weight of four skeletal muscles including gastrocnemius, rectus femoris, tibialis anterior, and pectoralis increased by 17.83 and 28.39%, 21.76 and 28.70%, 34.31 and 41.62%, 53.21 and 27.54% in transgenic male and female mice, respectively, compared to their corresponding non-transgenic control mice. Measurement of muscle fiber size and number indicated that the mean myofiber size increased by 50.73 and 61.30% in transgenic male and female mice respectively compared to the non-transgenic controls. However, there was no difference in the number of myofiber between transgenic and non-transgenic male mice. These results clearly demonstrated that the increase in skeletal muscle mass in transgenic mice is caused by hypertrophy instead of hyperplasia.

关键词： porcine myostatin muscle mass transgenic mice mutation hypertrophy hyperplasia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：