Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS

作     者：Yan Xiong Xiangfang Tang Qingshi Meng Hongfu Zhang 

作者机构：State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2016年第59卷第11期

页      面：1166-1176页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Science and Technology Support Program Project of China(2012BAD39B02) the Agricultural Science and Technology Innovation Program(ASTIP-IAS07) Institute Basic Scientific Research Funds(2015YWF-ZD-5) 

主　　题：ammonia broiler proteomics trachea 

摘      要：Ammonia has been considered the contaminant primarily responsible for respiratory disease in poultry. Even though it can cause tracheal lesions, its adverse effects on the trachea have not been sufficiently studied. The present study investigated tracheal changes in Arbor Acres broilers(Gallus gallus) induced by high concentration of ammonia using isobaric tag for relative and absolute quantification(iTRAQ)-based proteome analysis. In total, 3,706 proteins within false discovery rate of 1% were identified, including 119 significantly differentially expressed proteins. Functional analysis revealed that proteins related to immune response and muscle contraction were significantly enriched. With respect to the immune response, up-regulated proteins(like FGA) were pro-inflammatory, while down-regulated proteins participated in antigen processing and antigen presenting(like MYO1G), immunoglobulin and cathelicidin production(like fowlicidin-2), and immunodeficiency(like PTPRC). Regarding muscle contraction, all differentially expressed proteins(like TPM1) were up-regulated. An over-expression of mucin, which is a common feature of airway disease, was also observed. Additionally, the transcriptional alterations of 6 selected proteins were analyzed by quantitative RT-PCR. Overall, proteomic changes suggested the onset of airway obstruction and diminished host defense in trachea after ammonia exposure. These results may serve as a valuable reference for future interventions against ammonia toxicity.