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Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization

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Pedosphere 2008年第5期18卷 582-592页

作者： WEI Dan YANG Qian ZHANG Jun-Zheng WANG Shuang CHEN Xue-Li ZHANG Xi-Lin LI Wei-Qun Harbin institute of Technology Harbin 150001 （China）. The institute of Soil Fertility and Environmental Sources Heilongjiang Academy of Agricultural Sciences Harbin 150086（China）

Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin,Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments,suggesting that microorganisms with those bands were stable and not infiuenced by fertilization. However,chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments,suggesting that the application of manure increased the soil microbial population,but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments,but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria.

关键词：黑土长期施肥菌群结构化学肥料

CDC48B facilitates the intercellular trafficking of SHORT-ROOT during radial patterning in roots

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Journal of Integrative Plant Biology 2022年第4期64卷 843-858页

作者： Lihui Yang Mingyue Zhu Yi Yang Ke Wang Yulei Che Shurui Yang Jinxiang Wang Xin Yu Lixin Li Shuang Wu Klaus Palme Xugang Li Yong Wang State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityDaizong Street 61Tai'an271018 China institute of Biology II/Molecular Plant Physiology Faculty of BiologyAlbert-Ludwigs-University of FreiburgSchänzlestrasse 1FreiburgD-79104 Germany Department of Genetics Northwest Women's and Children's HospitalXi'an710061 China Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land Shandong Agricultural UniversityDaizong Street 61Tai'an271018 China Sino German Joint Research Center for agricultural Biology College of Life SciencesShandong Agricultural UniversityDaizong Street 61Tai'an271018 China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources&College of Natural Resources and Environment South China Agricultural UniversityGuangzhou510640 China Citrus Research institute Southwest UniversityChongqing400712 China Key Laboratory of Saline-alkali Vegetation Ecology Restoration Ministry of EducationCollege of Life SciencesNortheast Forestry UniversityHarbin150040 China FAFU-UCR Joint Center and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou350002 China 不详

CELL DIVISION CONTROL PROTEIN48(CDC48)is essential for membrane fusion,protein degradation,and other cellular ***,we revealed the crucial role of CDC48B in regulating periclinal cell division in roots by analyzing the recessive gen1 *** identified the GEN1 gene through map-based cloning and verified that GEN1 encodes ***1 showed severely inhibited root growth,increased periclinal cell division in the endodermis,defective middle cortex(MC)formation,and altered ground tissue patterning in *** with these phenotypes,CYCLIND 6;1(CYCD6;1),a periclinal cell division marker,was upregulated in gen1 compared to *** ratio of SHR_(pro):SHR-GFP fluorescence in pre-dividing nuclei versus the adjacent stele decreased by 33%in gen1,indicating that the trafficking of SHORT-ROOT(SHR)decreased in gen1 when endodermal cells started to *** findings suggest that the loss of function of CDC48B inhibits the intercellular trafficking of SHR from the stele to the endodermis,thereby decreasing SHR accumulation in the *** findings shed light on the crucial role of CDC48B in regulating periclinal cell division in roots.

关键词： Arabidopsis CDC48B cell division control periclinal cell division SHR

CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae

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Rice science 2022年第6期29卷 535-544,I0023-I0030页

作者： Fabiano T.P.KTÁVORA Anne Cécile MEUNIER Aurore VERNET Murielle PORTEFAIX Joëlle MILAZZO Henri ADREIT Didier THARREAU Octávio LFRANCO Angela MEHTA Department of Genetics and Biotechnology Federal University of Juiz de ForaJuiz de Fora-MG 36036330Brazil Embrapa Genetic Resources and Biotechnology Brasília-DF 70770-917Brazil Genetic Improvement and Adaptation of Mediterranean and Tropical Plants Agricultural Research Center for International Development(CIRAD)Montpellier 34398 CEDEX 5France CIRAD-INRAe-Agro institute University of MontpellierMontpellier 34000France Plant Health institute of Montpellier CIRADMontpellier 34398France University of Montpellier National Institute of Agronomic ResearchResearch Institute for DevelopmentMontpellier SupAgroMontpellier 34398France Department of Genomics science and Biotechnology Proteomics Analysis CenterCatholic University of BrasíliaBrasília-DF 71966-900Brazil S-Inova Biotech Catholic University of Dom BoscoCampo Grande-MS 79117-900Brazil

Rice genes OsDjA2 and OsERF104,encoding a chaperone protein and an APETELA2/ethylene-responsive factor,respectively,are strongly induced in a compatible interaction with blast fungus,and also have function in plant susceptibility validated through gene ***,we reported the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance.A total of 15 OsDjA2(62.5%)and 17 OsERF104(70.8%)T_(0)transformed lines were identified from 24 regenerated plants for each target and used in downstream *** of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area,compared with the infected control *** results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease.

关键词： gene editing plant-pathogen interaction Magnaporthe pathosystem plant immunity blast resistance S-gene rice

Water Absorption Capcapability and Moisture Content Seasonal Changing of Salt-Crust in Lop Nur Dry Lake

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Acta Geologica Sinica(English Edition) 2014年第S1期88卷 183-184页

作者： KONG Deyong LI Baoguo MA Lichun JIANG Ping'an WU Hongqi LIU Hongpeng College of Resources and Environment China Agricultural University institute of Mineral Resources Chinese Academy of Geological Sciences College of Grassland and Environment science Xinjiang Agricultural University

The Lop Nur dry salt lake,Xinjiang province,is charactered by typical physiognomy salt-crust,located *** and *** thickness of salt-crust is about from 20 cm to 100 cm,and

关键词： Lop Nur Dry Lake Salt-Crust Moisture Content Water Absorption Capcapability

Dynamic trends and driving forces of land use intensification of cultivated land in China

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Journal of Geographical sciences 2015年第1期25卷 45-57页

作者： WANG Guogang LIU Yansui LI Yurui CHEN Yangfen institute of agricultural Economics and Development CAAS Beijing 100081 China institute of Geographic sciences and Natural Resources Research CAS Beijing 100101 China

The aim of this study is to establish several important factors representing land use intensification in cultivated land （denoted by ClI）, using a multi-dimensional approach to achieve realistic and practical cultivated land use policies in China. For this reason, the theoretical framework was first built to explain the changes of land use intensification in the cultivated land, and then the variables and index were further developed for the purpose of characterizing the dynamic trends and driving forces of the land use intensification in the cultivated land at the provincial level. The study results indicate that the extent of Cll significantly increased during the period of 1996 to 2008, due to the extensive use of fertilizers, machinery and pesticide, increased labor and capital input, and intensified land use. Moreover, the principal component regression results show that the productivity of cultivated land, economic benefits of cultivated land, labor productivity, and land use conversion are the main factors affecting the village development. The first three factors play a positive role, while the last one has a negative effect on the land use intensification in the cultivated land. According to these results, the main policies for sustainable intensification in cultivated land are proposed. First, the sustainable pathways for intensification should be adopted to reduce the unsustainable uses of chemical fertilizer, agricultural chemicals, etc. Second, the conditions for agricultural production should be further improved to increase the cultivated land productivity. Third, it is very necessary and helpful for improving labor productivity and land use efficiency from the viewpoint of accelerated the cultivated land circulation. The last step is to positively affect the production activities of peasants by means of reforming the subsidy standards.

关键词： cultivated land land use intensification spatial pattern factors China

Grass Litter Decomposition Rate and Water-Holding Capacity in Dry-Hot Valley of Jinshajiang River

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Wuhan University Journal of Natural sciences 2009年第1期14卷 92-96页

作者： ZHANG Mingzhong ZHU Hongye JIN Jie SHI Liangtao SHA Yucang institute of Tropical Eco-agricultural sciences Yunnan Academy of Agriculture Sciences Yuanmou 651300 Yunnan China science and Technological Division Yunnan Academy of Agricultural Sciences Kunming 650231 Yunnan China

The decomposition rate and the water-holding capacity of 6 kinds of grass litters were studied in the Jinshajiang river dry-hot valley. The results showed that the dry weight remains reduced with time but did not have a direct correlation with time. The decomposition litters were affected by climate, character of litter, animalcule and soil animal. The climate factors of temperature and humidity were important especially. The water-holding capacity was in the sequence ofN. wightii （336%）, P. notatum Flugge （248%）, H. contortus （L.） Beauv （209%）, B. pertusa （L.） A. Camus（206%）, L endecaphylla Jacq （174%）, D. annulatum （Forsk.） Stapf （168%）. After 24 months decomposition the remaining dry weight of the litter was in the sequence as follows： 15.12% for N. wightii, 26.38% for I. endecaphylla Jacq, 27.23% for B. pertusa （L.） A. Camus, 30.78% for P. notatum Flugge, 39.72% for H. contortus （L.） Beauv and 39.76% for D. annulatum （Forsk.） Stapf. The decomposition rate and water-holding capacity ofN. wightii are at the highest level. It is important for the development, improvement and conservation of the grassland soil.

关键词： grass litter dry-hot river valley decomposition rate water- holding capacity

Evaluation of the Pathogenicity of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant in Piglets

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agricultural sciences in China 2011年第8期10卷 1280-1291页

作者： WEI Tian-chao TIAN Zhi-jun ZHOU Yan-jun AN Tong-qing JIANG Yi-feng XIAO Yan HU Shou-ping PENG Jin-mei HAO Xiao-fang ZHANG Shan-rui TONG Guang-zhi State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001P.R.China Division of Swine Infectious Diseases Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai 200241P.R.China College of Animal science and Technology Guangxi UniversityNanning 530005P.R.China

Since May 2006,a highly pathogenic porcine reproductive and respiratory syndrome virus （HP-PRRSV） variant characterized by 30 amino acids deletion within its NSP2-coding region emerged and caused extensive economic losses to China＇s pig *** investigate the in vivo pathogenicity and immune responses of the newly emerging PRRSV,3 groups of 60-d-old conventional piglets were inoculated intranasally with a representative strain of the HP-PRRSV variant HuN4 with 3 different infection doses （3×103-3×105 TCID50）.The results revealed that the virus variant caused severe disease in piglets and the significant clinical characteristics consisted of persistently high fever （41.0-41.9oC） and high morbidity and mortality （60-100%）,the marked clinical signs of PRRS and severe histopathogenic damages in multiple *** induced rapid and intense humoral immune responses and seroconversion was detected in most infected pigs at 7 d post-infection （DPI）.The virus vigorously replicated in vivo and the highest virus average titer was 9.7 log copies mL-1 serum at 7 *** levels of IFN-g and IL-10 cytokine production in serum in this study were also *** together,our results demonstrated that the HP-PRRSV variant HuN4 strain is highly pathogenic for piglets and suitable to be a reference strain of highly virulent PRRSV for evaluating the efficacy of the new vaccines.

关键词： highly pathogenic porcine reproductive and respiratory syndrome virus pathogenicity immune responses viral load

Transcription factor ZmNAC126 plays an important role in transcriptional regulation of maize starch synthesis-related genes

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The Crop Journal 2021年第1期9卷 192-203页

作者： Qianlin Xiao Yayun Wang Hui Li Chunxia Zhang Bin Wei Yongbin Wang Huanhuan Huang Yangping Li Guowu Yu Hanmei Liu Junjie Zhang Yinghong Liu Yufeng Hu Yubi Huang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Agronomy and Biotechnology Southwest UniversityChongqing 400716China College of Agronomy Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Life science Sichuan Agricultural UniversityYa'an 625014SichuanChina Maize Research institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Sichuan Seed Station Chengdu 610041SichuanChina

Maize(Zea mays L.)is one of the most important food crops in the world,and starch is the main component of its *** regulation plays a vital role in starch ***,it is not well understood in *** report the identification of the transcription factor ZmNAC126 and its role in regulation of starch synthesis in *** expression of ZmNAC126 was higher in maize endosperm and kernels than in roots or ***126 shared a similar expression pattern with starch synthesis genes during seed development,and its expression pattern was also consistent with the accumulation of ***126 is a typical transcription factor with a transactivation domain between positions 201 and 227 of the amino acid sequence,is located in the nucleus,and binds to CACG repeats in *** one-hybrid assay revealed that ZmNAC126 bound the promoters of ZmGBSSI,ZmSSIIa,ZmSSIV,ZmISA1,and *** overexpression of ZmNAC126 in maize endosperm increased the activities of promoters pZmSh2,pZmBt2,pZmGBSSI,pZmSSIIIa,and pZmBT1 but inhibited the activities of pZmISA1 and ***126 thus acts in starch synthesis by transcriptionally regulating targeted starch synthesis-related genes in maize kernels.

关键词： Maize Starch synthesis ZmNAC126 Co-expression Transcription regulation

Carbon sequestration of plantation in Beijing-Tianjin sand source areas

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Journal of Mountain science 2018年第10期15卷 2148-2158页

作者： LIU Xiu-ping ZHANG Wan-jun CAO Jian-sheng YANG Bai CAI Yan-jiang Key Laboratory of agricultural Water Resources Hebei Key Laboratory of Agricultural Water-Saving Center for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Environmental sciences Division Oak Ridge National Laboratory Key Laboratory of Mountain Environment Evolvement and Regulation Institute of Mountain Hazards and Environment Chinese Academy of Sciences

The Beijing-Tianjin Sand Source Control Project(BTSSCP), a national ecological restoration project, was launched to construct an ecological protection system in the Beijing-Tianjin sand source areas to reduce dust hazards. The carbon sequestration dynamics can be used to assess the ecological effects of an ecological restoration project. Here, we conducted vegetation and soil study to assess the carbon sequestration in the plantations with 10 years old stands in Beijing-Tianjin sand source areas. The results at the site scales indicated that the average net increase of plantation ecosystem carbon stock was 33.8 Mg C ha^(-1), with an annual increase rate of 3.38 Mg C ha^(-1) yr^(-1). The average net increase of carbon varied among regions, vegetation types, and forest management activities. Soil bulk density in the top soil decreased slightly after 10-year implementation of the project. Coniferous forests and shrubs are suitable plant species for sand source *** restoration in the plantations is a practical and feasible and promising approach for enhancing ecosystem carbon sequestration potential.

关键词：种植园天津北京源区碳生态系统控制工程恢复工程