Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization

作     者：WEI Dan YANG Qian ZHANG Jun-Zheng WANG Shuang CHEN Xue-Li ZHANG Xi-Lin LI Wei-Qun 

作者机构：Harbin Institute of Technology Harbin 150001 （China）. The Institute of Soil Fertility and Environmental Sources Heilongjiang Academy of Agricultural Sciences Harbin 150086（China） 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2008年第18卷第5期

页      面：582-592页

核心收录：

学科分类：07[理学] 0818[工学-地质资源与地质工程] 0903[农学-农业资源与环境] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：the Heilongjiang Provincial Natural Science Funds for Distinguished Young Scholars, China (No.JC200622) the Heilongjiang Provincial Natural Science Foundation of China (No.C2004-17) the National "Eleventh Five Years Plan" Key Project on Science and Technology of China (No.2006BAD25B05) 

主　　题：黑土 长期施肥 菌群结构 化学肥料 

摘      要：Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin,Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments,suggesting that microorganisms with those bands were stable and not infiuenced by fertilization. However,chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments,suggesting that the application of manure increased the soil microbial population,but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments,but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria.