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Synthetic and Systems biotechnology 2021年第2期6卷 69-76页

作者： Pingping Wang Jiali Wang Guoping Zhao Xing Yan Zhihua Zhou CAS-Key laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of Sciences300 Fenglin RdShanghai200032China School of Life Sciences Henan UniversityKaifeng475001China

Ginsenoside Compound K(CK)has been recognized as a major functional component that is absorbed into the systemic circulation after oral administration of *** demonstrates diverse bioactivities.A phase I clinical study indicated that CK was a potential candidate for arthritis ***,a phase II clinical study was suspended because of the high cost associated with the present CK manufacturing approach,which is based on the traditional planting-extracting-biotransforming *** previously elucidated the complete CK biosynthetic pathway and realized for the first time de novo biosynthesis of CK from glucose by engineered ***,CK production was not sufficient for industrial ***,we systematically engineered Saccharomyces cerevisiae to achieve high titer production of CK from glucose using a previously constructed protopanaxadiol(PPD)-producing chassis,optimizing UGTPg1 expression,improving UDP-glucose biosynthesis,and tuning down UDP-glucose *** final engineered yeast strain produced CK with a titer of 5.74 g/L in fed-batch fermentation,which represents the highest CK production in microbes reported to *** scaled-up,this high titer de novo microbial biosynthesis platform will enable a robust and stable supply of CK,thus facilitating study and medical application of CK.

关键词： Compound K UDP-Glucose Glycosylation efficiency Cell factory Synthetic biology

Manipulating magnetic anisotropies of Co/MgO(001) ultrathin films via oblique deposition

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Chinese Physics B 2016年第9期25卷 551-555页

作者： Syed Sheraz Ahmad 何为汤进张永圣胡泊叶军 Qeemat Gul 张向群成昭华 State Key laboratory of Magnetism and Beijing National laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of Sciences

We present a systematic investigation of magnetic anisotropy induced by oblique deposition of Co thin films on MgO（001） substrates by molecular beam epitaxy at different deposition angles,i.e.,0？,30？,45？,60？,and 75？with respect to the surface *** energy electron diffraction（LEED）,surface magneto–optical Kerr effect（SMOKE）,and anisotropic magnetoresistance（AMR） setups were employed to investigate the magnetic properties of cobalt *** values of in-plane uniaxial magnetic anisotropy（UMA） constant Ku and four-fold magnetocrystalline anisotropy constant K1 were derived from magnetic torque curves on the base of AMR *** was found that the value of Ku increases with increasing deposition angle with respect to the surface normal,while the value of K_1 remains almost constant for all the ***,by using MOKE results,the Ku values of the films deposited obliquely were also derived from the magnetization curves along hard *** results of AMR method were then compared with that of hard axis fitting method（coherent rotation） and found that both methods have almost identical values of UMA constant for each sample.

关键词： magnetic anisotropy oblique deposition cobalt ultrathin film anisotropic magnetoresistance

Low-Temperature Signaling Pathways and Their Signaling Factors in Plant

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Agricultural Sciences 2023年第2期14卷 256-268页

作者： Jianping Li Zhiqing Liu Xiaoyan Hao Xiaochun Chang Zhun Zhao Guo Chen Wenran Hu Shengqi Gao Quansheng Huang Institute of Nuclear Technology and biotechnology Xinjiang Academy of Agricultural Sciences Urumqi China Xinjiang Key laboratory of Crop biotechnology Urumqi China Institute of Economic Crops Xinjiang Academy of Agricultural Sciences Urumqi China

Low temperature as abiotic stress adversely impacts plant growth and development, and limits the ecological distribution of plants as well. Throughout their long evolutionary history, plants have developed a range of complicated and precise molecular regulatory mechanisms to deal with low-temperature stress, involving the activation of signal transduction pathways and the regulation of related genes. In this review, we provide a systematic summary of the most recent research findings regarding three hypotheses of cellular perception of low-temperature signals and two major intracellular low-temperature signaling pathways, including CBF-dependent signaling pathways and CBF-independent signaling pathways. Focus is placed on the functions of each component of the ICE-CBF-COR signaling cascade as well as their interrelationships. This review concludes that although some progress has been made in the identification, function, and mechanism of low-temperature response genes, their roles in the low-temperature regulatory network and molecular mechanisms still need to be studied in detail, which will be of great significance for improving the low-temperature tolerance of plants and adapting to climate change.

关键词： Low Temperature Signaling CBF COR Signaling Factors

Extension of Highly Efficient Alcohol-promoted Rearrangement into One-pot Domino Palladium-catalyzed Carbonylation for Sterically Crowded Aromatic Six-membered Imides

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Chemical Research in Chinese Universities 2023年第6期39卷 1106-1110页

作者： GUO Rong ZHANG Jingmei JIA Xiaohui SHI Chunfeng QIE Fengxiang ZHEN Yonggang Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic CompositesBeijing University of Chemical TechnologyBeijing 100029P.R.China State Key laboratory of Catalytic Materials and Reaction Engineering Research Institute of Petroleum ProcessingSINOPECBeijing 100083P.R.China National Demonstration Center for Experimental Life Sciences&biotechnology Education Beijing Normal University100875 BeijingP.R.China Wuhan National laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074P.R.China State Key laboratory of Optoelectronic Materials and Technologies Sun Yat-sen UniversityGuangzhou 510275P.R.China State Key laboratory of Fine Chemicals Dalian University of TechnologyDalian 116024P.R.China

Aromatic six-membered imides suffer the introduction of bulky substituents at the imide positions due to the formation of isoimides and low reactivity,preventing from the investigation of the intrinsic molecular ***,we found a highly efficient alcohol-promoted rearrangement from isonaphthalimide to naphthalimide under acid or basic nonaqueous conditions,which can be regarded as model compounds for aromatic six-membered *** proposed two-step nucleophilic substitutions in the isomerization mechanistic pathways that were verified by the separation of a key ***,in-situ ^(1)H NMR exhibited the first-order kinetics for the isoimide loss ***,the alcohol-promoted isoimide-imide rearrangement was extended to a palladium-catalyzed one-pot domino carbonylation reaction toward sterically crowded aromatic six-membered imides.

关键词： Bulky aromatic imide Imide isomerization Domino synthesis Organic semiconductor

Towards a natural classification and backbone tree for Sordariomycetes

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Fungal Diversity 2015年第3期72卷 199-301页

作者： Sajeewa S.N.Maharachchikumbura Kevin D.Hyde EBGareth Jones Eric H.C.McKenzie Shi-Ke Huang Mohamed A.Abdel-Wahab Dinushani A.Daranagama Monika Dayarathne Melvina J.D’souza Ishani D.Goonasekara Sinang Hongsanan Ruvishika S.Jayawardena Paul M.Kirk Sirinapa Konta Jian-Kui Liu Zuo-Yi Liu Chada Norphanphoun Ka-Lai Pang Rekhani H.Perera Indunil C.Senanayake Qiuju Shang Belle Damodara Shenoy Yuanpin Xiao Ali H.Bahkali Jichuan Kang Sayanh Somrothipol Satinee Suetrong Tingchi Wen Jianchu Xu Guizhou Key laboratory of Agricultural biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Key laboratory for Plant biodiversity and Biogeography of East Asia(KLPB) Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina World Agroforestry Centre East and Central Asia132 Lanhei RoadKunming 650201China Institute of Excellence in Fungal Research and School of ScienceMae Fah Luang UniversityChiang Rai 57100Thailand Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 11451Kingdom of Saudi Arabia Manaaki Whenua Landcare Research Private Bag 92170AucklandNew Zealand The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China Guizhou UniversityGuiyang 550025Guizhou ProvincePeople’s Republic of China Department of Botany Faculty of ScienceSohag UniversitySohag 82524Egypt Institute of Marine Biology and Centre of Excellence for the Oceans Taiwan Ocean University2 Pei-Ning RoadKeelung 20224TaiwanRepublic of China Mycology Section Royal Botanic GardensKewLondonUK Microbe Interaction laboratory(BMIT) National Center for Genetic Engineering and Biotechnology(BIOTEC)113 Thailand Science ParkPhahonyothin RoadKhlong NuengKhlong LuangPathum ThaniThailand Fungal Diversity laboratory(BFBD) National Center for Genetic Engineering and Biotechnology(BIOTEC)113 Thailand Science ParkPhahonyothin RoadKhlong NuengKhlong LuangPathum ThaniThailand Biological Oceanography Division CSIR-National Institute of OceanographyDona PaulaGoa 403004India Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo 9 of ShuGuangHuaYuanZhangLuHaidian DistrictBeijing 100097China

Sordariomycetes is one of the largest classes of Ascomycota and is characterised by perithecial ascomata and inoperculate unitunicate *** class includes many important plant pathogens,as well as endophytes,saprobes,epiphytes,and fungicolous,lichenized or lichenicolous *** class includes freshwater,marine and terrestrial taxa and has a worldwide *** paper provides an updated outline of the Sordariomycetes and a backbone tree incorporating asexual and sexual genera in the *** on phylogeny and morphology we introduced three subclasses;Diaporthomycetidae,Lulworthiomycetidae and Meliolomycetidae and five orders;Amplistromatales,Annulatascales,Falcocladiales,Jobellisiales and *** outline is based on literature to the end of 2014 and the backbone tree published in this *** for 397 taxa with information,such as new family and genera novelties,novel molecular data published since the Outline of Ascomycota 2009,and new links between sexual and asexual genera and thus synonymies,are *** Sordariomycetes now comprises six subclasses,28 orders,90 families and 1344 *** addition a list of 829 genera with uncertain placement in Sordariomycetesis also provided.

关键词： Boliniales Calosphaeriales Chaetosphaeriales Coniochaetales Coronophorales Diaporthales Glomerellales Hypocreomycetidae Hypocreales Koralionastetales Lulworthiales Magnaporthales Melanosporales Meliolales Microascales molecular identification Nomenclature Ophiostomatales Phyllachorales Sordariales Sordariomycetidae Taxonomy Trichosphaeriales Type species Xylariales Xylariomycetidae

Development and characterization of wheat–Aegilops kotschyi 1U^(k)(1A)substitution line with positive dough quality parameters

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Journal of Integrative Agriculture 2023年第4期22卷 999-1008页

作者： JIANG Yun WANG De-li HAO Ming ZHANG Jie LIU Deng-cai State Key laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130P.R.China biotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural SciencesChengdu 610061P.R.China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130P.R.China

Exploring novel high molecular weight glutenin subunits(HMW-GSs)from wild related species is a strategy to improve wheat processing *** objective of the present investigation was to identify the chromosomes of the wheatalien introgression line N124,derived from the hybridization between Triticum aestivum with Aegilops kotschyi,and characterize the effects on quality-related *** in situ hybridization karyotypes showed that N124 is a disomic 1U^(k)(1A)substitution *** dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and reversedphase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the *** *** RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were U^(k)x and U^(k)*** to the wheat parent,the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality *** can be served as a donor or bridge material for wheat quality improvement.

关键词： Aegilops kotschyi common wheat 1Uk(1A)substitution line HMW-GS processing quality

Bacteriological Quality and Risk Factors for Contamination of Raw Mixed Vegetable Salads Served in Collective Catering in Abidjan (Ivory Coast)

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Advances in Microbiology 2017年第6期7卷 405-419页

作者： Evelyne Toe Adjéhi Dadié Etienne Dako Guillaume Loukou laboratory of Nutrition and Food Safety Department of Food Science and Technology University of Nanguy Abrogoua Abidjan Ivory Coast laboratory of biotechnology and molecular Biology School of Food Science Nutrition and Family Studies Faculty of Health Science and Community Services University of Moncton New-Brunswick Canada Laboratoire Nationale de la Santé Publique (LNSP) Abidjan Ivory Coast

The aim of this study was to determine the bacteriological quality of raw mixed vegetable salads served in collective catering in Abidjan and to assess the factors likely to favor contamination. An investigation was conducted to determine the practical risk of contamination. A total of 306 samples were collected and analyzed for the detection and enumeration of Enterobacteriaceae, E. coli and Salmonella according to standard microbiology methods. The most important risk factors were lack of training (96.7%), decontamination (84.0%), cross-contamination of vegetables in contact with fresh chicken or fish (76.7%) and salads that were uncovered and unprotected, thus exposed to the air and at ambient temperature for sale (97.7%). Theprevalence was 100%, 77.8% and 2.6% respectively for Enterobacteriaceae, E. coli and Salmonella. The average load of Enterobacteriaceae and E. coli was 4.93 log10 (CFU/g) and 1.89 log10 (CFU/g), respectively. Unsatisfactory microbiological quality samples were 52.3%. The results showed a failure of the sanitary quality in more than half of the products. Raising awareness in all of the workers in the food chain for vegetable salads is thus necessary to protect the health of the consumers.

关键词： Vegetable Salads E. coli Salmonella Risk Factors Collective Catering

Quantitative heterogeneity and subgroup classification based on motility of breast cancer cells

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Chinese Physics B 2019年第10期28卷 590-596页

作者： Ling Xiong Yanping Liu Ruchuan Liu Wei Yuan Gao Wang Yi He Jianwei Shuai Yang Jiao Xixiang Zhang Weijing Han Junle Qu Liyu Liu Chongqing Key laboratory of Soft Condensed Matter Physics and Smart Materials College of PhysicsChongqing UniversityChongqing 401331China Department of Physics Xiamen UniversityXiamen 361005China Key laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen UniversityShenzhen 518060China Materials Science and Engineering Arizona State UniversityTempeArizona 85287USA Division of Physical Science and Engineering King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Shenzhen Shengyuan biotechnology Co. LtdShenzhen 518060China

Cancer cell motility and its heterogeneity play an important role in metastasis, which is responsible for death of 90% of cancer patients. Here, in combination with a microfluidic technique, single-cell tracking, and systematic motility analysis,we present a rapid and quantitative approach to judge the motility heterogeneity of breast cancer cells MDA-MB-231 and MCF-7 in a well-defined three-dimensional(3D) microenvironment with controllable conditions. Following this approach,identification of highly mobile active cells in a medium with epithelial growth factor will provide a practical tool for cell invasion and metastasis investigation of multiple cancer cell types, including primary cells. Further, this approach could potentially become a speedy(~ hours) and efficient tool for basic and clinical diagnosis.

关键词： cancer metastasis motility heterogeneity three-dimensional microenvironment invasion potential

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

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Science China(Life Sciences) 2008年第3期51卷 280-285页

作者： LI Ang1,2, CHEN LiangLiang1,3, REN HaiYun3, WANG XueChen2, ZHANG HaiWen1,4 & HUANG Rong- Feng1,4 1 biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2 National laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China 3 College of Life Sciences, Beijing Normal University, Beijing 100875, China 4 National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing China National laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing China College of Life Sciences Beijing Normal University Beijing China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing China

In rice, the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further de- termine the crucial sequences responsible for MeJA induction, we generated a series of deletion pro- moters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is po- sitioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (?1127), which is distinct from the essential region containing ERE (?562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

关键词： OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves

The Structural Proteins of Bdellovibrio bacteriovorus Bacteriophage MAC-1

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World Journal of Engineering and Technology 2016年第3期4卷 7-13页

作者： Rajinder S. Ranu Renee Gallegos Mary Althauser Lisa Wolfe laboratory of Plant molecular Biology & biotechnology Department of Bioagricultural Sciences & Pest Management Colorado State University Fort Collins CO USA Department of Microbiology Colorado State University Fort Collins CO USA Proteomics & Metabolomics Facility Colorado State University Fort Collins CO USA

In the present investigation the structural proteins associated with MAC-1 bacteriophage have been characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE);tandem mass spectrometry of protein bands from SDS-PAGE gel;from the open reading frames (ORFs) deduced from MAC-1 genome sequence and amino acid sequence homology searches from the Uniprot database (up000002418). Results have led to the identification of at least three structural proteins associated with MAC-1 phage genome. They are: capsid protein (~55,000-daltons);spike protein (~22,000-daltons) and a low molecular weight DNA binding protein (~4000-dal- tons). In addition, two other minor proteins were tentatively identified as replicative and scaffold proteins based on two to three unique peptides from mass spectrometry data. However, other proteins coded (ORFs) by phage genome remain to be identified.

关键词： Bdellovibrio bacteriovorus Bacteriophage MAC-1 Phage Structural Proteins MAC-1 Genome MAC-1 Genes (ORFs)