The Structural Proteins of Bdellovibrio bacteriovorus Bacteriophage MAC-1
The Structural Proteins of Bdellovibrio bacteriovorus Bacteriophage MAC-1作者机构：Laboratory of Plant Molecular Biology & Biotechnology Department of Bioagricultural Sciences & Pest Management Colorado State University Fort Collins CO USA Department of Microbiology Colorado State University Fort Collins CO USA Proteomics & Metabolomics Facility Colorado State University Fort Collins CO USA
出 版 物：《World Journal of Engineering and Technology》 (世界工程和技术（英文）)
年 卷 期：2016年第4卷第3期
页 面：7-13页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
主 题：Bdellovibrio bacteriovorus Bacteriophage MAC-1 Phage Structural Proteins MAC-1 Genome MAC-1 Genes (ORFs)
摘 要：In the present investigation the structural proteins associated with MAC-1 bacteriophage have been characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE);tandem mass spectrometry of protein bands from SDS-PAGE gel;from the open reading frames (ORFs) deduced from MAC-1 genome sequence and amino acid sequence homology searches from the Uniprot database (up000002418). Results have led to the identification of at least three structural proteins associated with MAC-1 phage genome. They are: capsid protein (~55,000-daltons);spike protein (~22,000-daltons) and a low molecular weight DNA binding protein (~4000-dal- tons). In addition, two other minor proteins were tentatively identified as replicative and scaffold proteins based on two to three unique peptides from mass spectrometry data. However, other proteins coded (ORFs) by phage genome remain to be identified.
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