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Role of selenoprotein M knockdown in the melatonin antagonism of nickel-induced apoptosis and endoplasmic reticulum stress in mouse heart

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第5期24卷 406-417页

作者： Xintong ZHANG Xiaoxue GAI Lihua XU Wenxue MA Qiaohan LIU Bendong SHI Cheng FANG Jingzeng CAI Ziwei ZHANG College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030China suihua agricultural and rural bureau Suihua 152000China College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030China4.Key Laboratory of the Provincial EducationDepartment of Heilongjiang for Common Animal Disease Prevention and TreatmentNortheast Agricultural UniversityHarbin 150030China

The aim of this study was to investigate the role of selenoprotein M(SelM)in endoplasmic reticulum stress and apoptosis in nickel-exposed mouse hearts and to explore the detoxifying effects of *** 21 d after intraperitoneal injection of nickel chloride(NiCl_(2))and/or melatonin into male wild-type(WT)and SelM knockout(KO)C57BL/6J mice,NiCl_(2)was found to induce changes in the microstructure and ultrastructure of the hearts of both WT and SelM KO mice,which were caused by oxidative stress,endoplasmic reticulum stress,and apoptosis,as evidenced by decreases in malondialdehyde(MDA)content and total antioxidant capacity(T-AOC)*** in the messenger RNA(mRNA)and protein expression of genes related to endoplasmic reticulum stress(activating transcription factor 4(ATF4),inositol-requiring protein 1(IRE1),c-Jun N-terminal kinase(JNK),and C/EBP homologous protein(CHOP))and apoptosis(B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),Caspase-3,Caspase-9,and Caspase-12)were also ***,the observed damage was worse in SelM KO ***,melatonin alleviated the heart injury caused by NiCl_(2)in WT mice but could not exert a good protective effect in the heart of SelM KO ***,the findings suggested that the antioxidant capacity of SelM,as well as its modulation of endoplasmic reticulum stress and apoptosis,plays important roles in nickel-induced heart injury.

关键词： Selenoprotein M(SelM) Nickel chloride(NiCl_(2)) Melatonin Apoptosis Mouse heart

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An Integrated Quantitative Trait Locus Map of Oil Content in Soybean,Glycine max(L.) Merr.,Generated Using a Meta-Analysis Method for Mining Genes

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agricultural Sciences in China 2011年第11期10卷 1681-1692页

作者： QI Zhao-ming HAN Xue SUN Ya-nan WU Qiong SHAN Da-peng DU Xiang-yu LIU Chun-yan JIANG Hong-wei HU Guo-hua CHEN Qing-shan Soybean Research Institute Northeast Agricultural University Harbin 150030 P.R. China Crop Research and Breeding Center of Land-Reclamation Harbin 150090 P.R.China Heilongjiang Academy of agricultural Sciences Suihua Institute Suihua 152052 P.R.China

Soybean is a major cash crop in the world, and its oil content was one of the very important traits. Therefore, the study of gene mapping for oil content in soybean is very important for breeding application. At present, at least 130 QTL loci for soybean oil content have been published; however, the mapping results of oil content were dispersed and a coalescent public map should be established to integrate the published QTLs, and to more efficiently mine genes based on the meta- analysis method of the bioinformatics tools. This study was to construct an integrated map of QTLs for soybean oil content and accelerate the application of bioinformation resource related to oil content improvement in the practice of soybean breeding. We collected information of 130 QTLs reported over the past 20 yr for soybean oil content and used the Software BioMercator 2.1 to project QTLs from their own maps onto a reference map, which was an early-integrated map constructed by Song （2004） for oil-content quantitative trait loci （QTLs） in soybean. Gene mining was performed based on the meta-analysis by running the local ver. GENSCAN and InterProScan. The confidence interval of QTLs was efficaciously narrowed using the meta-analysis method, and 25 consensus QTLs were mapped on the reference map. Using a local version of GENSCAN, 12 805 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted sequences were aligned to gene sequences from the International Protein Index database using InterProScan locally. Thirteen predicted genes were in the class of the geme ontology （GO） accession （0006631）, which were involved in the fatty acid metabolic process. These genes were analyzed using BLAST at the NCBI website to examine whether they were related to oil content. Six genes were found in the oil-synthesis pathway. Twenty-five consensus QTLs and six genes were found in the oil-synthesis pathway. These results would lay the foundation for marker-assisted selection and

关键词： soybean oil content meta-analysis consensus QTL gene ontology (GO)

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Research on DSP-Based Automatic Excitation Regulator in Small rural Hydropower Station

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Journal of Northeast agricultural University(English Edition) 2013年第1期20卷 65-69页

作者： Gu Xiang-yu Zhao Yu-lin Fu Yan Zhang Yu-juan Sun Shuai College of Electrical Engineering and Information Northeast Agricultural University suihua rural Power bureau School of Science Harbin University Shizuishan Power Supply bureau

Excitation regulator has played an important role to ensure that generator run safely and stably. However, in some remote areas, the rural small generator excitation control system is very backward, and it has hindered the development of Chinese agriculture and the pace of new countryside construction. This paper introduced a kind of structure and basic principle of DSP-based automatic excitation regulator, which was applied to rural small generator. We chose TMS320LF2407A chip as the core of regulator control system; the regulator adjusted PWM based on PID control strategy to control the exciting current. The theoretical analysis showed that the regulator had the characteristics of simplicity and reliability, well dynamic and static characteristics, and it could also adjust its own state quickly. The result was applicable to small rural hydropower station.

关键词： DSP PID control strategy automatic excitation regulator new countryside construction

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Oxygen Electroreduction on CoSe2 Nanoparticles Prepared via Hydrothermal Method in Acidic Medium

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Chemical Research in Chinese Universities 2015年第3期31卷 447-451页

作者： ZHAO Dongjiang MA Songyan School of Food and Pharmaceutical Engineering Suihua University Suihua 152061 P. R. China

CoSe2 nanoparticles were synthesized via a facile hydrothermal method, and characterized by means of X-ray diffraction（XRD）, scanning electron microscopy（SEM） and transmission electron microscopy（TEM）. The syn- thesized CoSe2 was composed of crystalline orthorhombic phase and displayed a morphology of short and thin nano- belts. The results of the catalyst experiment demonstrate that the CoSe2 nanocrystals show high catalytic activity and methanol tolerance in oxygen reduction reaction（OR_K） with an open circuit potential（OCP） of 0.80 V（vs. NHE） in 0.5 mol/L HzSO4 at 25 ℃. The transfer process of about 3.7 electrons per oxygen molecule was determined during the reduction process and the transfer coefficient and Tafel slope were 0.50 and 118 mV, respectively, in the potential region of 0.64-0.75 V（vs. NHE）. The high catalytic activity might be related to the high crystallization of the CoSe2 powder and the modification of selenium on the cobalt element.

关键词： Oxygen reduction Cobalt selenide Non-noble metal catalyst Polymer electrolyte membrane fuel cell

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Characterization of chromosome segment substitution lines reveals candidate genes associated with the nodule number in soybean

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Journal of Integrative Agriculture 2022年第8期21卷 2197-2210页

作者： ZOU Jia-nan ZHANG Zhan-guo KANG Qing-lin YU Si-yang WANG Jie-qi CHEN Lin LIU Yan-ru MA Chao ZHU Rong-sheng ZHU Yong-xu DONG Xiao-hui JIANG Hong-wei WU Xiao-xia WANG Nan-nan HU Zhen-bang QI Zhao-ming LIU Chun-yan CHEN Qing-shan XIN Da-wei WANG Jin-hui College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China suihua Branch of Heilongjiang Academy of agricultural Sciences Suihua 152000P.R.China Soybean Research Institute Jilin Academy of Agricultural SciencesChangchun 130033P.R.China Jiamusi Branch of Heilongjiang Academy of agricultural Sciences Jiamusi 154000P.R.China

Soybean is one of the most important food crops *** other legumes,soybean can form symbiotic relationships with Rhizobium *** fixation of soybean via its symbiosis with Rhizobium is pivotal for sustainable *** Ⅲ effectors(T3Es)are essential regulators of the establishment of the symbiosis,and nodule number is a feature of nitrogen-affected ***,genes encoding T3Es at quantitative trait loci(QTLs)related to nodulation have rarely been *** segment substitution lines(CSSLs)have a common genetic background but only a few loci with heterogeneous genetic information;thus,they are suitable materials for identifying candidate genes at a target *** this study,a CSSL population was used to identify the QTLs related to nodule number in *** nucleotide polymorphism(SNP)markers and candidate genes within the QTLs interval were detected,and it was determined which genes showed differential expression between *** candidate genes(GmCDPK28,GmNAC1,GmbHLH,and GmERF5)linked to the SNPs were identified as being related to nodule traits and pivotal processes and pathways involved in symbiosis establishment.A candidate gene(GmERF5)encoding a transcription factor that may interact directly with the T3E NopAA was *** confirmed CSSLs with important segments and candidate genes identified in this study are valuable resources for further studies on the genetic network and T3Es involved in the signaling pathway that is essential for symbiosis establishment.

关键词： soybean TypeⅢeffectors nodule number chromosome segment substitution lines

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Hydrothermal Synthesis and Crystal Structure of a New Supramolecular Channel Framework Based on Cu Complexes and Polyoxovanadate:[Cu(I)(phen)_2]_5[HV_(15)O_(36)(Cl)]_(0.65)[H_3V_(16)O_(38)(Cl)]_(0.35)·2H_2O

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Chinese Journal of Structural Chemistry 2006年第7期25卷 830-836页

作者： LIU Hong-Sheng CHEN Yan-Hui PENG Jun SHI Zhen-Yu DONG Bao-Xia KONG Yu-Mei Institute of Polyoxometalate Chemistry Department of Chemistry Northeast Normal University Changchun Jilin 130024 China Department of Chemzstry Suihua University Suihua Heilongjiang 152061 China

A new compound [Cu（I）（phen）2]5[HV15O36（Cl）]0.65[H3V16O38（Cl）]0.35·2H2O 1 （phen = 1,10′-phenanthroline） has been prepared from the hydrothermal reaction of V2O5, CuCl, phen, H2C2O4·2H2O, THMAM （tris（hydroxymethyl）aminomethane） and H2O in the molar ratio of 3：1：1：0.5：2.5：1400. Compound 1 crystallizes in the space group P42212 of the tetragonal system with a = b = 21.5009（8）, c = 28.401（2） A, V= 13129.5（12） A^3 Z = 4, Dc = 1.800 g/cm^3, μ（MoKa） = 1.932 mm^-1, λ（MoKa） = 0.71073 A, F（000） = 7063.4, C120H81.20ClCu5N20O38.7V15.35, Mr = 3557.58, the final R = 0.0594 and wR= 0.1320 for 7761 observed reflections （I 〉 2σ（I））. Structure analysis shows that 1 exhibits a 3D channel framework formed by the supramolecular assembly of Cu complexes and polyoxovanadate clusters via intermolecular hydrogen bonding, and the channels contain Cu complexes. Other characterizations by elemental analysis, XPS, IR, XRD and thermal analysis are also described.

关键词： hydrothermal synthesis crystal structure polyoxovanadate supramolecular assembly

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Genetic Variation Dissection of Rice Blast Resistance Using an Indica Population

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Rice science 2020年第4期27卷 255-258页

作者： ZHANG Mengchen WEI Zhonghua YUAN Xiaoping WANG Caihong WANG Shan NIU Xiaojun XU Xin XU Qun FENG Yue YU Hanyong WANG Yiping ZHU Zhiwei ZHAI Rongrong YANG Yaolong WEI Xinghua State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China suihua Branch of Heilongjiang Academy of agricultural Sciences Suihua 152000China Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021China

Rice blast disease,caused by fungus pathogen Magnaporthe oryzae,is one of the most destructive diseases that impact rice *** the worldwide,rice harvests lose 10%to 30%of the total production because of blast infection... 详细信息

关键词： Population enough farming

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Several Suggestions on the Climate Change and Its Studies

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Meteorological and Environmental Research 2011年第8期2卷 60-62页

作者： GAO Hong-jiao GAO Qing-ling WANG An-na LIU Li-yan suihua Meteorological bureau of Heilongjiang Province Suihua 152002China

According to the abundant studies,the relevant information and comprehensive analysis of the climate changes,several important problems on the climate changes and its studies were *** on the temporal distribution of the meteorological disaster of agriculture,the wave theory was expounded so as to draw people’s attention on climate changes and to be objective,just and careful about the study.

关键词： Climate changes Discussion Climate prediction Wave theory Suggestion China

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Synthesis,Crystal Structure and Luminescence of a Novel One-dimensional Silver(Ⅰ)Sulfonate Complex with Pyrazine Ligand

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Chinese Journal of Structural Chemistry 2011年第2期30卷 211-215页

作者：刘海燕迟云超孙红梅 Department of Chemistry and Pharmaceutical Engineering Suihua University

The title complex,[Ag 2 （L1） 2 （Pyr）·2.5H 2 O] 1 （L1=6-amino-1-naphthalenesulfonate anion,Pyr=pyrazine）,has been synthesized and characterized by single-crystal X-ray diffraction *** crystallizes in triclinic,space group P1,with a=7.339（5）,b=13.105（5）,c=14.097（5）,α=84.377（5）,β=76.921（5）,γ=79.628（5）°,V=1296.7（11） 3,C 48 H 50 Ag 4 N 8 O 17 S 4,M r=1570.68,Z=1,D c=2.011 g/cm 3,μ=1.732 mm-1,F（000）=782,S=1.148,the final R=0.0436 and wR=0.1181 for 4130 observer reflections （I 2σ（Ⅰ））.Compound 1 shows a rare one-dimensional （1D） double chain,and the chains are further connected through secondary bonding,hydrogen bonds,and π-π interactions to generate a three-dimensional （3D） supramolecular *** luminescent property of 1 was also studied in the solid state at room temperture.

关键词： silver(Ⅰ) sulfonate pyrazine coordination polymer luminescence

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Atmospheric pollution of agriculture-oriented cities in Northeast China: A case in suihua

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Journal of Environmental Sciences 2020年第11期32卷 85-95页

作者： Lili Li Kun Wang Weiwei Chen Qingliang Zhao Lijuan Liu Wei Liu Yang Liu Junqiu Jiang Jiumeng Liu Mengduo Zhang State Key Laboratory of Urban Water Resources and Environment School of EnvironmentHarbin Institute of TechnologyHarbin150090China Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun130102China suihua Ecological Environment Monitoring Center Suihua152000China Heilongjiang Provincial Environmental Science Research Institute Harbin150090China

Agriculture-oriented cities in Northeastern China have experienced frequent atmospheric pollution *** understandings of the pollution characteristics,haze causes and effects of management on local air quality are crucial for conducting integrated management approaches for the sustainable development of agriculture-oriented *** a typical agriculture-dominant city(i.e.,suihua)in Northeast China,we analyzed in detail the characteristics and causes of atmospheric pollution and evaluated the straw-burning prohibition using multisource *** results showed a clear temporal pattern of air quality index(AQI)on an annual scale(i.e.,2015-April 2019),with two typical pollution periods occurring in late autumn and early *** large areas of concentrated straw burning at local and regional scales accounted for the first period(i.e.,October and November),while dust emissions and farming disturbances comprised the second *** interannual variation in pollution periods among these years was large,showing similar trends from 2015 to 2017 and the postponed late-autumn pollution period in *** evaluation has shown that the prohibition effect of straw burning significantly improved air quality in 2018,with a reduction of 59%±88%in the PM2.5 concentrations in October and November compared to 2015–***,From October to April of the following year,the improvement effect was not significant due to postponement of straw burning to February or *** analysis also highlighted the roles of meteorological conditions,Therefore,combined with the promotion of straw utilization,scientifically prescribed burning considering the burning amount and location,meteorological conditions and regional transportation should be implemented.

关键词： Pollution characteristics Haze causes Straw burning prohibition Agriculture-oriented Northeast China

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