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Mutations in Spike Gene of SARS-CoV-2 that Are Associated with a Higher Viral Load: A Clinical Case Study

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Case Reports in Clinical Medicine 2022年第11期11卷 474-486页

作者： Ibrahim Keita Garan Dabo Lassina Doumbia Youssouf Diarra Ibrahim Traoré Mariam Traoré Lansana Sangaré Yacouba Toloba Mahamadou Alpha Diallo Djeneba Sy Andres H. Gutierrez Aliou Sissako Ibrahim Guindo Mohamed Diallo Akory Ag Iknane Anne S. De Groot Ousmane Aliou Koita Laboratoire de Biologie Moléculaire Appliquée Université des Sciences Techniques et des Technologies de Bamako Bamako Mali Hô pital du Mali Ministère de la Santé Publique et du Développement Social Bamako Mali Centre Hospitalo-Universitaire du Point G Ministère de la Santé Publique et du Développement Social Bamako Mali EpiVax Inc. Providence USA Institut National de Santé Publique Ministère de la Santé Publique et du Développement Social Bamako Mali Center for Vaccines and Immunology College of Veterinary Medicine University of Georgia Athens USA

Background: Quantitative PCR (qPCR) can be used to detect and quantify a load of a pathogen. It is a good indicator of the degree of transmissibility. While performing routine qPCR, we observed an unusually short cycle threshold (Ct) value of SARS-CoV-2 for a clinical specimen obtained in Bamako, Mali. This prompted us to sequence the short-cycle SARS-CoV-2 sample to identify potential mutations in the Spike gene (S gene) gene. Methods: Post-infection, Quantitative Reverse Transcription (qRT-PCR) was performed over a defined time course to estimate the Ct of the SARS-CoV-2 specimen collected from the patient. Sanger sequencing was done on the entire fragment of the S gene to identify mutations. Findings: Sanger sequencing revealed mutations in the lineage of interest, designated B.1.525 by Pango, and also known as “Eta” using the nomenclature defined by WHO. This variant was originally found in Nigeria and Italy. The four novel mutations identified in Eta (D228N, Y451N, I1172M, and C1250F) were otherwise observed with a low frequency worldwide. Although the initial Ct was 10 in the case study patient, he did not exhibit severe symptoms of SARS-CoV-2, for example, pneumonia. However, we observed a longer viral clearance period than usual, of 3 weeks. We note that as compared to SARS-CoV-2 samples obtained during the first peaks of SARS-CoV-2 infection in Mali, when the infection was at its peak in March 2020 (Ct = 30.4), circulating strains evaluated at the time the Eta sample was obtained demonstrated a lower mean Ct (Ct = 24). Conclusions: The short cycle threshold associated with this variant, and the temporal association with a decrease in the mean Ct in the region of Bamako, may indicate higher levels of transmissibility due to a circulating variant. This variant is a lineage of interest designated B.1.525 by Pango or Eta by WHO.

关键词： SARS-CoV-2 Variant Eta Ct Value Mali

改变一皮制粉条件对通心粉和面粉物化成分的影响

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郑州工程学院学报 2001年第4期22卷 1-7页

作者：时予新 G A Hareland ProjectManagerBuhler InC.MinneapolisMN55440-9497USA ResearchFoodTechnologist USDA/ARSWheatQualityLaboratory.HarrisHall NDSUFargoND58105USA

主要研究了不同磨齿齿数、排列、速比以及入磨麦水分对于杜伦麦在一皮系统中物化成分的影响 .对于在一皮产生的通心粉 (Semolina)和面粉的出率、灰分、蛋白含量、淀粉含量、淀粉破损进行了测定 .分析了通心粉与面粉之间量的关系 ,以及... 详细信息

主要研究了不同磨齿齿数、排列、速比以及入磨麦水分对于杜伦麦在一皮系统中物化成分的影响 .对于在一皮产生的通心粉 (Semolina)和面粉的出率、灰分、蛋白含量、淀粉含量、淀粉破损进行了测定 .分析了通心粉与面粉之间量的关系 ,以及琥珀色与其他品质参数之间的关系 .从统计结果来看 ,增加齿数使通心粉和面粉的出率显著增加 ,同时他们的淀粉破损也显著增加 .通心粉的灰分也显著增加而面粉的灰分却显著降低 .通心粉的淀粉含量显著降低而面粉的淀粉含量却显著增加 .钝对钝排列 ,使面粉出率显著增加 .提高速比使通心粉和面粉的出率、灰分和蛋白含量都显著增加 .随着入磨麦水分的提高 ,通心粉和面粉的出率、蛋白含量及淀粉破损都显著降低 .通心粉和面粉的质量在各项测定指标中呈现出统计学意义上的显著不同 (P<0 .0 0 1 ) .通心粉的琥珀色 (黄色 )与入磨水分呈负相关而与通心粉蛋白含量呈正相关 .

关键词：制粉杜伦麦通心粉面粉物化特性

Integrated Behavioral Healthcare in Thailand:A Case Study from Chiang Mai

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Psychosomatic Medicine Research 2022年第1期4卷 19-27页

作者： Ronald O’Donnell Shiyou Wu Patraporn Bhatarasakoon Jennifer Rolfes Decha Tamdee Erik P Turtle Kasia Gilpatrick Kaoru Sato Breanna Reeser Mohamed Abdalla Rodger Kessler College of Health Solutions Arizona State UniversityArizona StateU.S.A School of Social Work Arizona State UniversityArizona StateU.S.A Faculty of Nursing Chiang Mai UniversityChiang MaiThailand Cornerstone Whole Healthcare Organization Inc.U.S.A US Department of Justice U.S.A Metro Dermatology of NY&NJ U.S.A Integrated Health Initiatives Care1stU.S.A Global Center for Applied Health Research Arizona State UniversityArizona StateU.S.A Department of Family Medicine School of MedicineUniversity of ColoradoColorado StateUSA

Purpose:This study aims to examine the level of behavioral health integration in primary care clinics among patients with hypertension and type 2 diabetes in Chiang Mai,*** also aims to explore the provider perceived prevalence of behavioral problems and need for an expert behavioral health consultant on the primary care team and the risk and protective factors of the patients’psychological and cardio-metabolic health ***:Using survey data between 2017 and 2018 regarding the level of integration as measured by a Practice Integration Profile,provider ratings of prevalence and need based on a Provider Survey,and patient lifestyle and behavioral problems with a Health Risk Assessment in six primary health care settings in Chiang Mai,Thailand(n=335).We conducted ordinary least squares regressions to explore the relationship between each of the patient psychological and cardio-metabolic health outcomes and physical,mental health and substance abuse variables while controlling for the ***:The results showed overall high levels of integration,but significant variability across *** rated prevalence of health risk and need for a Behavioral Health Consultant as *** of the patient health risk assessment were variable,with some problems high severity,others ***:The results reflect a need for integrated behavioral health into the health care system in Thailand especially for those who are in the chronic illness *** for future study inc.ude an evaluation of the translation quality and validity and reliability of the study measures under the Thai *** study fills the research gap of lacking research on the level of integrated care in clinics in Thailand.

关键词： Integrated Behavioral Health Integrated healthcare Noncommunicable diseases Hypertension Diabetes Patient health risk

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历史地震损失重建研究

世界地震工程 2006年第3期22卷 10-13页

作者：郭恩栋王自法 Chris M. Weimin D. 中国地震局工程力学研究所 Risk Management Solutions.inc.CA94560 USA

历史地震损失重建是地震损失预测的一种方法,也是用于区域地震风险分析模型验证的一种手段。在介绍了历史地震损失重建一般方法的基础上,对方法中等效全损住宅比例估计方法以及住宅结构地震易损性指数的估计方法进行了重点分析,给出了... 详细信息

历史地震损失重建是地震损失预测的一种方法,也是用于区域地震风险分析模型验证的一种手段。在介绍了历史地震损失重建一般方法的基础上,对方法中等效全损住宅比例估计方法以及住宅结构地震易损性指数的估计方法进行了重点分析,给出了这两个关键参数的可选择的多种估计方法。

关键词：历史地震损失重建

Effectiveness of boron neutron capture therapy for recurrent head and neck malignancies

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中国口腔颌面外科杂志 2008年第B5期6卷 79-80页

作者： Itsuro Kato Yusei Fujita Akitoshi Kamida Akira Maruhashi Yoshinofi Sakurai Hiroaki Kumada Masatoshi Ohmae Mitsunori Kifihata Yoshio Imahori Tetsuro Sumi Souichi Iwai Mitsuhiro Nakazawa Koji Ono Department of Oral and Maxillofacial Surgery 11 Osaka University Graduate School of Dentistry Suita Osaka 5650871 Japan Radiation Oncology Osaka 590-494 Japan Research Reactor Institute Research Reactor Institute School of Medicine Sapporo Medical University Hokkaido 060-8556 Japan JapanAtomic Energy Agency Tokai Research and Development Center lbaraki Japan Department of Oral and Maxillofacial Surgery Izumisano Municipal Hospital Rinku General Hospital lzumisao Osaka 598-0048 Japan Graduate School of Agriculture and Life Sciences Osaka Prefecture University Osaka 599-8531 Japan CEO of Cancer Intelligence Care System Inc. Tokyo 135-0063 Japan

An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

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Virologica Sinica 2022年第1期37卷 138-141页

作者： Ying Tang Yiqin Wang Yuchang Li Huai Zhao Sen Zhang Ying Zhang Jing Li Yuehong Chen Xiaoyan Wu Chengfeng Qin Tao Jiang Xiaoping Kang Department of Virology Beijing Institute of Microbiology and EpidemiologyAMMSBeijing 100071China State Key Laboratory of Pathogen and Biosecurity AMMSBeijing 100071China Beijing University of Chemical Technology Beijing 100029China Technology Center of Erenhot Customs Erenhot 011100China Lifereal Biotech.inc. Hangzhou 311100China Anhui Medical University Hefei 230032China

Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-Co V-2)is a novel coronavirus that causes the outbreak of coronavirus disease 2019(COVID-19)(Li et al.,2020a).Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19(Wu et al.,2020a;Zhu et al.,2020).Currently,a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection(Corman et al.,2020;Hussein et al.,2020;Ruhan et al.,2020;Veyer et al.,2020).Conventional qPCR involves virus inactivation,nucleic acid extraction,and qPCR amplification ***,the process is complicated,which usually takes longer than 2 h,and requires biosafety laboratories and professional ***,qPCR is not suitable for use in field or medical units.

关键词： al. diagnosis acute

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减少凸轮轴淬火变形的新型技术

减少凸轮轴淬火变形的新型技术

第十一次全国热处理大会

作者： Valery Rudnev Randy Minnick Glen Desmier Inductoheat inc 应达集团

感应热处理是最常用的汽车零部件热处理方式,如淬火汽车发动机的关键零部件——凸轮轴,现在介绍一种几乎没有淬火变形的新的凸轮轴热处理技术。

关键词：凸轮轴淬火变形感应热处理

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电磁超声技术及其在焊缝无损检测中的应用

电磁超声技术及其在焊缝无损检测中的应用

基于HFC有线网络的运营级WiFi无线接入网的性能需求分析及其网络架构

2010远东无损检测新技术论坛

作者：高会栋 Borja Lopez Innerspec Technologies Inc

电磁超声技术利用洛伦兹力或者是磁致伸缩原理在金属或者是磁性材料中激发出超声波,它是一种超声无损检测新技术。相对于其他无损检测技术而言,电磁超声具有所有的超声检测的优势。声波的激发发生在材料的表面而不是在传感器内部。所以...

电磁超声技术利用洛伦兹力或者是磁致伸缩原理在金属或者是磁性材料中激发出超声波,它是一种超声无损检测新技术。相对于其他无损检测技术而言,电磁超声具有所有的超声检测的优势。声波的激发发生在材料的表面而不是在传感器内部。所以它与传统的基于压电换能器的超声检测相比,具有无需耦合剂,可非接触,可适用于超低温或超高温环境,有利于工业自动化,以及可方便有效的实现横波检测及导波检测等优势。Innerspec Technologies公司致力于电磁超声技术的研发和应用,在近十几年中开发了150多台工业在线电磁超声检测系统。应用领域包括钢铁,汽车制造,石油天然气管道,压力容器等领域。作为一种超声波技术,电磁超声可以应用于各种厚度测量,缺陷检测以及材料定征。本文主要利用几个实例来讨论电磁超声在焊缝检测中的应用。第一个应用是利用导波对激光拼焊板的检测。第二个应用是电阻焊钢管的在线检测。我们利用了沿着周向传播的超声导波对焊缝进行检测,克服了由于焊缝位置偏转对传统超声焊缝检测设备带来的困难。第三个应用是水平横波以及多通道电磁超声相控阵技术实现奥氏体不锈钢焊缝的检测。

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基于HFC有线网络的运营级WiFi无线接入网的性能需求分析及其网络...