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Lipid droplets in the nervous system:involvement in cell metabolic homeostasis

Neural Regeneration Research 2025年第3期20卷 740-750页

作者： Yuchen Zhang Yiqing Chen Cheng Zhuang Jingxuan Qi Robert Chunhua Zhao Jiao Wang School of Life Sciences Shanghai UniversityShanghaiChina School of Medicine Shanghai UniversityShanghaiChina Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic MedicinePeking Union Medical CollegeBeijingChina Center of Excellence in Tissue Engineering Chinese Academy of Medical SciencesBeijingChina Beijing Key Laboratory of New Drug Development and Clinical Trial of stem cell therapy BeijingChina

Lipid droplets serve as primary storage organelles for neutral lipids in neurons,glial cells,and other cells in the nervous *** droplet formation begins with the synthesis of neutral lipids in the endoplasmic ***,lipid droplets were recognized for their role in maintaining lipid metabolism and energy homeostasis;however,recent research has shown that lipid droplets are highly adaptive organelles with diverse functions in the nervous *** addition to their role in regulating cell metabolism,lipid droplets play a protective role in various cellular stress ***,lipid droplets exhibit specific functions in neurons and glial *** of lipid droplet formation leads to cellular dysfunction,metabolic abnormalities,and nervous system *** review aims to provide an overview of the role of lipid droplets in the nervous system,covering topics such as biogenesis,cellular specificity,and ***,it will explore the association between lipid droplets and neurodegenerative *** the involvement of lipid droplets in cell metabolic homeostasis related to the nervous system is crucial to determine the underlying causes and in exploring potential therapeutic approaches for these diseases.

关键词： Alzheimer's disease lipid droplet biogenesis lipid droplets lipid metabolism nervous system neurodegenerative disorders oxidative stress Parkinson's disease

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How to package and SEND mRNA: a novel “humanized” vector system based on endogenous retroviruses

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Signal Transduction and Targeted therapy 2021年第12期6卷 3367-3368页

作者： Kristoffer Riecken Dawid Głów Boris Fehse Research Dept.cell&Gene therapy Dept.of Stem Cell TransplantationUniversity Medical Centre Hamburg-Eppendorf(UKE)Hamburg 20246Germany

A recent publication in Science describes a novel mRNA delivery system termed SEND,entirely consisting of endogenous proteins.1 These virus-like particles(VLPs),able to deliver mRNA of e.g.,Cas9,represent a new class of gene transfer *** development of efficient,specific,and safe vectors for the delivery of nucleic acids and proteins remains one of the biggest challenges in gene therapy,particularly for in vivo *** currently preferred viral vectors have several limitations,including biosafety issues,laborious and expensive manufacture,as well as the impossibility of multiple applications due to the inherent immunogenicity of viral *** contrast,non-viral vectors are much easier to produce on a large scale,but have not yet reached gene transfer rates comparable with viral vectors.

关键词： endogenous system entirely

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for stem cells School of Life Sciences and TechnologyTongji University Translational Medical Center for stem cell therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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Advances in the treatment of autism spectrum disorder:Wharton jelly mesenchymal stem cell transplantation

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World Journal of Methodology 2025年第1期15卷 72-79页

作者： Serdar Kabatas ErdinçCivelek Eyüp Can Savrunlu Umut Karaaslan Özlem Yıldız Erdal Karaöz Department of Neurosurgery University of Health Sciences TurkeyGaziosmanpaşa Training and Research HospitalIstanbul 34255Türkiye Department of Pediatric Allergy-Immunology Institute of Health Sciences Marmara UniversityIstanbul 34865Türkiye Center for stem cell&Gene therapy Research and Practice University of Health Sciences TurkeyIstanbul 34255Türkiye Department of Child and Adolescent Psychiatry University of Health SciencesGaziosmanpaşa Training and Research HospitalIstanbul 34255Türkiye Department of Child and Adolescent Psychiatry Kocaeli University Faculty of MedicineKocaeli 41001Türkiye Center for Regenerative Medicine and stem cell Research&Manufacturing Liv HospitalIstanbul 34340Türkiye Department of Histology and Embryology Istinye UniversityFaculty of MedicineIstanbul 34010Türkiye Center for stem cell and Tissue Engineering Research and Practice Istinye UniversityIstanbul 34340Türkiye

BACKGROUND Autism spectrum disorder(ASD)is a complex neurodevelopmental disorder with multifaceted *** recent studies,neuroinflammation and immune dysregulation have come to the forefront in its *** are studies suggesting that stem cell therapy may be effective in the treatment of *** To evolve the landscape of ASD treatment,focusing on the potential benefits and safety of stem cell *** A detailed case report is presented,displaying the positive outcomes observed in a child who underwent intrathecal and intravenous Wharton’s jelly-derived mesenchymal stem cells(WJ-MSCs)transplantation combined with *** The study demonstrates a significant improvement in the child’s functional outcomes(Childhood Autism Rating Scale,Denver 2 Developmental Screening Test),especially in language and gross motor *** serious side effects were encountered during the 2-year *** The findings support the safety and effectiveness of WJ-MSC transplantation in managing ASD.

关键词： Autism spectrum disorder Neurorehabilitation stem cell transplantation Wharton jelly mesenchymal stem cells Inflammation

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Outcomes of haploidentical bone marrow transplantation in patients with severe aplastic anemia-II that progressed from non-severe acquired aplastic anemia

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Frontiers of Medicine 2021年第5期15卷 718-727页

作者： Hongchen Liu Xiaoli Zheng Chengtao Zhang Jiajun Xie Beibei Gao Jing Shao Yan Yang Hengxiang Wang Jinsong Yan Department of Hematology Liaoning Medical Center for Hematopoietic Stem Cell TransplantationDalian Key Laboratory of HematologyLiaoning Key Laboratory of Hematopoietic Stem Cell Transplantation and Translational MedicineDiamond Bay Institute of Hematologythe Second Hospital of Dalian Medical UniversityDalian 116027China Department of Hematology Air Force Medical CenterPLABeijing 100142China Department of Environmental Health and Toxicology Liaoning Medical Center for Hematopoietic Stem Cell TransplantationLiaoning Key Laboratory of Hematopoietic Stem Cell Transplantation and Translational MedicineSchool of Public HealthDalian Medical UniversityDalian 116044China

Severe aplastic anemia II(SAA-II)progresses from non-severe aplastic anemia(NSAA).The unavailability of efficacious treatment has prompted the need for haploidentical bone marrow transplantation(haplo-BMT)in patients lacking a human leukocyte antigen(HLA)-matched *** study aimed to investigate the efficacy of haplo-BMT for patients with ***-two patients were included and followed up,and FLU/BU/CY/ATG was used as conditioning *** these patients,21 were successfully engrafted,19 of whom survived after *** patients experienced grade II–IV aGvHD,including two with grade III–IV *** patients experienced chronic GvHD,among whom four were mild and two were *** patients experienced infections during *** was diagnosed with post-transplant lymphoproliferative disorder and one with probable EBV disease,and both recovered after rituximab ***-BMT achieved 3-year overall survival and disease-free survival rate of 86.4%±0.73%after a median follow-up of 42 months,indicating its effectiveness as a salvage *** promising outcomes may support haplo-BMT as an alternative treatment strategy for patients with SAA-II lacking HLA-matched donors.

关键词： severe aplastic anemia non-severe acquired aplastic anemia haploidentical bone marrow transplantation outcomes

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Electrochemiluminescence immunoassay method underestimates cortisol suppression in ulcerative colitis patients treated with oral prednisone

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World Journal of Gastroenterology 2014年第31期20卷 10895-10899页

作者： Francesco Manguso Raffaele Bennato Giovanni Lombardi Assunta Viola Elisabetta Riccio Livio Cipolletta Division of Gastroenterology Cardarelli Hospital Division of Haematology and stem cell transplantation Unit Cardarelli Hospital

AIM: To evaluate cortisolemia by using conventional electrochemiluminescence immunoassay (ECLIA) method compared to liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in active ulcerative colitis (UC) pa... 详细信息

关键词： Cortisol Immunoassay Liquid chromatography Prednisone Tandem mass spectrometry Ulcerative colitis

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An effective inducer of dopaminergic neuron-like differentiation

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Neural Regeneration Research 2013年第5期8卷 427-434页

作者： Wenyu Fu Cui Lv Wenxin Zhuang Dandan Chen E Lv Fengjie Li Xiaocui Wang Department of Histology and Embryology Weifang Medical University stem cell Research and transplantation Center Affiliated Hospital of Weifang Medical University

Rat bone marrow-derived mesenchymal stem cells were cultured and passaged in vitro. After induction with basic fibroblast growth factor for 24 hours, passage 3 bone marrow-derived mesenchymal stem cells were additionally induced into dopaminergic neurons using three different combinations with basic fibroblast growth factor as follows： 20% Xiangdan injection; ali-trans retinoic acid ＋ glial-derived neurotrophic factor; or sonic hedgehog ＋ fibroblast growth factor 8. Results suggest that the bone marrow-derived mesenchymal stem cells showed typical neuronal morphological characteristics after induction. In particular, after treatment with sonic hedgehog ＋ fibroblast growth factor 8, the expressions of nestin, neuron-specific enolase, microtubule- associated protein 2, tyrosine hydroxylase and vesicular monoamine transporter-2 in cells were significantly increased. Moreover, the levels of catecholamines in the culture supernatant were significantly increased. These findings indicate that Xiangdan injection, all-trans retinoic acid ＋ glial-derived neurotrophic factor, and sonic hedgehog ＋ fibroblast growth factor 8 can all induce dopaminergic neuronal differentiation from bone marrow-derived mesenchymal stem cells. In particular, the efficiency of sonic hedgehog ＋ fibroblast growth factor 8 was highest.

关键词： neural regeneration stem cells mesenchymal stem cells dopaminergic neuron induction differentiation Xiangdan injection all-trans retinoic acid sonic hedgehog fibroblast growth factor 8 catecholamine grants-supported paper photographs-containing paper neuroregeneration

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A pancreatic player in dementia:pathological role for islet amyloid polypeptide accumulation in the brain

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Neural Regeneration Research 2023年第10期18卷 2141-2146页

作者： Angelina S.Bortoletto Ronald J.Parchem Center for cell and Gene therapy Stem Cell and Regenerative Medicine CenterDepartment of NeuroscienceDepartment of Molecular and Cellular BiologyTranslational Biology and Molecular Medicine ProgramMedical Scientist Training ProgramBaylor College of MedicineHoustonTXUSA

Type 2 diabetes mellitus patients have a markedly higher risk of developing *** multiple factors contribute to this predisposition,one of these involves the increased secretion of amylin,or islet amyloid polypeptide,that accompanies the pathophysiology of type 2 diabetes *** amyloid polypeptide accumulation has undoubtedly been implicated in various forms of dementia,including Alzheimer’s disease and vascular dementia,but the exact mechanisms underlying islet amyloid polypeptide’s causative role in dementia are *** this review,we have summarized the literature supporting the various mechanisms by which islet amyloid polypeptide accumulation may cause neuronal damage,ultimately leading to the clinical symptoms of *** discuss the evidence for islet amyloid polypeptide deposition in the brain,islet amyloid polypeptide interaction with other amyloids implicated in neurodegeneration,neuroinflammation caused by islet amyloid polypeptide deposition,vascular damage induced by islet amyloid polypeptide accumulation,and islet amyloid polypeptide-induced *** are very few therapies approved for the treatment of dementia,and of these,clinical responses have been controversial at ***,investigating new,targetable pathways is vital for identifying novel therapeutic strategies for treating *** such,we conclude this review by discussing islet amyloid polypeptide accumulation as a potential therapeutic target not only in treating type 2 diabetes mellitus but as a future target in treating or even preventing dementia associated with type 2 diabetes mellitus.

关键词： Alzheimer’s disease amylin amyloid dementia diabetes human islet amyloid polypeptide islet amyloid polypeptide protofibrils type 2 diabetes mellitus vascular dementia

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Androgen receptor signaling-mitochondrial DNA-oxidative phosphorylation:A critical triangle in early prostate cancer

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Current Urology 2022年第4期16卷 207-212页

作者： Minas Sakellakis Laura Jacqueline Flores Department of Genitourinary Oncology MD Anderson Cancer CenterUniversity of TexasHoustonTXUSA Department of stem cell transplantation and cellular therapy MD Anderson Cancer CenterUniversity of TexasHoustonTXUSA

Mitochondria are more than just the cellular *** also play key roles in vital functions such as apoptosis,metabolism regulation,and other intracellular *** mitochondrial DNA(mtDNA)encodes for 12 subunits of the oxidative phosphorylation(OXPHOS)*** of mtDNA in androgen-dependent prostate cancer(PCa)cell lines renders them androgen-independent and more ***,pharmaceutical inhibition of OXPHOS is lethal for subsets of PCa cells,whereas others become dependent on androgen receptor(AR)signaling for *** that the AR-mitochondria interaction is critical for early PCa,it is crucial to understand the details of this *** hurdles have made mitochondria traditionally difficult to study,with many techniques used for isolation masking the properties of given individual *** the isolation of mitochondria enables us to study OXPHOS,we miss the context in which mitochondria interact with the rest of the *** AR signaling and mtDNA affect apoptosis,metabolism regulation,cellular calcium storage and homeostasis,intracellular calcium signaling,and redox *** this review,we will attempt to understand how the crosstalk between AR-mtDNA-OXPHOS is responsible for"life or death"decisions inside the *** aim is to point toward potential vulnerabilities that can lead to the discovery of novel therapeutic targets.

关键词： Androgen receptor Mitochondria Oxidative phosphorylation Prostate cancer

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Liquid biopsies for liquid tumors: emerging potential of circulating free nucleic acid evaluation for the management of hematologic malignancies

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Cancer Biology & Medicine 2016年第2期13卷 215-225页

作者： Jay Hocking Sridurga Mithraprabhu Anna Kalff Andrew Spencer Myeloma Research Group Australian Center for Blood Diseases Monash University Malignant Haematology & stem cell transplantation Service Alfred Hospital

Circulating free nucleic acids; cell free DNA and circulating micro-RNA, are found in the plasma of patients with hematologic and solid malignancies at levels higher than that of healthy individuals. In patients with hematologic malignancy cell free DNA reflects the underlying tumor mutational profile, whilst micro-RNAs reflect genetic interference mechanisms within a tumor and potentially the surrounding microenvironment and immune effector cells. These circulating nucleic acids offer a potentially simple, non-invasive, repeatable analysis that can aid in diagnosis, prognosis and therapeutic decisions in cancer treatment.

关键词： Circulating nucleic acids DNA miRNA hematologic malignancy biopsy

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