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应用物联网技术加速畜禽精准饲喂:从概念到实施

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Science Bulletin 2024年第14期69卷 2156-2160页

作者：苏家宜谭碧娥蒋宗勇吴德 C.M.Nyachoti Sung Woo Kim 印遇龙王婧 key laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Institute of Animal Science Guangdong Academy of Agricultural SciencesGuangzhou 510640China key laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China and Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130China Department of Animal Science University of ManitobaWinnipeg R3T 2N2Canada Department of Animal Science North Carolina State UniversityRaleigh 27695USA laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-Ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410128China Yuelushan laboratory Changsha 410128China

Maximizing sow reproductive performance is vital for ensuring healthy piglet supply,feed efficiency,and profitability,which requires understanding factors such as nutrition,disease control,genetics,herd management,and their *** is well documented that most reproductive issues in sows stem from extreme fluctuations in body reserves[1].Thus,optimizing reproduction should prioritize regulating body reserves and making continuous nutritional adjustments throughout a sow’s life[1,2].However,the traditional one-size-fits-all feeding strategy may not meet individual sow needs under varying *** feeding strategy leads to nutrient under-or over-feeding,which may result in a lack of performance and health issues on the one hand,and economic loss and negative environmental effects on the other.

关键词： feeding extreme reserves

Diverse functions of GmNLA1 members in controlling phosphorus homeostasis highlight coordinate response of soybean to nitrogen and phosphorus availability

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The Crop Journal 2023年第4期11卷 1251-1260页

作者： Ming Zhou Yaxue Li Xing Lu Panmin He Cuiyue Liang Jiang Tian Root Biology Center State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesCollege of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642GuangdongChina

Nitrogen(N) and phosphorus(P) are two essential mineral nutrients for plant growth,which are required in relative high amount in *** have evolved a series of strategies for coordinately acquiring and utilizing N and ***,physiological and molecular mechanisms underlying of N and P interactions remain largely unclear in soybean(Glycine max).In this study,interactions of N and P were demonstrated in soybean as reflected by significant increases of phosphate(Pi) concentration in both leaves and roots by N deficiency under Pi sufficient conditions.A total of four nitrogen limitation adaptation(NLA),encoding RING-type E3 ubiquitin ligase were subsequently identified in soybean *** them,transcription of Gm NLA1-1 and Gm NLA1-3 was decreased in soybean by N starvation under Pi sufficient conditions,not for Gm *** of all three Gm NLA1 members was able to increase Pi concentration regardless of the P and N availability in the growth medium,but decrease fresh weight under normal conditions in soybean hairy ***,comparted to changes in control lines at two N levels,N deficiency only resulted in a relatively higher increase of Pi concentration in Gm NLA1-1 or Gm NLA1-3 suppression lines,strongly indicating that Gm NLA1-1 and Gm NLA1-3 might regulate P homeostasis in soybean response to N *** together,our result suggest that redundant and diverse functions present in Gm NLA1 members for soybean coordinate responses to P and N availability,which mediate P homeostasis.

关键词： GmNLA1 N availability P deficiency P homeostasis Soybean

Sequence analysis of Erianthus arundinaceus chromosome 1 isolated by flow sorting after genomic in situ hybridization in suspension

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The Crop Journal 2022年第6期10卷 1746-1754页

作者： Shan Yang Petr Cápal Jaroslav Dolezel Xueting Li Wang Qian Zhiqiang Wang Kai Zeng Peiting Li Hongkai Zhou Rui Xia Muqing Zhang Zuhu Deng National Engineering Research Center for Sugarcane&state key laboratory for conservation and utilization of subtropical agro-bioresources Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina state key laboratory for conservation and utilization of subtropical agro-bioresources&Guangxi key laboratory of Sugarcane Biology Guangxi UniversityNanning 530004GuangxiChina Institute of Experimental Botany of the Czech Academy of Sciences Center of the Region Hanáfor Biotechnological and Agricultural ResearchOlomouc CZ-77900Czech Republic College of Coastal Agricultural Sciences Guangdong Ocean UniversityZhanjiang 524088GuangdongChina state key laboratory for conservation and utilization of subtropical agro-bioresources South China Agricultural UniversityGuangzhou 510642GuangdongChina

Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic ***,limited knowledge of the complex genome of hexaploid *** has impeded the development of required molecular *** complex genomes into single chromosomes can simplify *** flow-cytometric sorting of a single chromosome of *** in a Saccharum-Erianthus introgression line is reported.A novel approach called genomic in situ hybridization in suspension was used to discriminate the alien chromosome from sugarcane chromosomes at the same size.A total of 218,000 *** chromosome 1(EaC1)were sorted to>97%purity and amplified DNA was sequenced using Illumina and Pac Bio *** resulting assembly had a 70.93 Mb contig sequence with an N50 of 19.62 kb.A total of 56.69 Mb repeat sequences were predicted,accounting for 79.1%of the chromosome and 2646 genes having a total length of 1.84 Mb that represented 2.59%of the *** these genes,1877(70.9%)genes were functionally *** phylogenetic relationship of *** with other species using the chromosome1 sequence revealed that *** was distantly related to Oryza sativa and Zea mays,followed by Sorghum bicolor,and was closely related to *** and Saccharum *** study provides the first insights into the characteristics of EaC1,and the results will provide tools to support molecular improvement and alien introgression breeding of sugarcane.

关键词： Flow cytometry GISHIS Chromosome sorting Genome analysis Sugarcane

Interplay between ethylene and nitrogen nutrition:How ethylene orchestrates nitrogen responses in plants

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Journal of Integrative Plant Biology 2023年第2期65卷 399-407页

作者： Biao Ma Tian Ma Wenhao Xian Bin Hu Chengcai Chu Guangdong laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China

The stress hormone ethylene plays a key role in plant adaptation to adverse environmental ***(N)is the most quantitatively required mineral nutrient for plants,and its availability is a major determinant for crop *** in N availability or N forms can alter ethylene biosynthesis and/or *** serves as an important cellular signal to mediate root system architecture adaptation,N uptake and translocation,ammonium toxicity,anthocyanin accumulation,and premature senescence,thereby adapting plant growth and development to external N ***,we review the ethylenemediated morphological and physiological responses and highlight how ethylene transduces the N signals to the adaptive *** specifically discuss the N-ethylene relations in rice,an important cereal crop in which ethylene is essential for its hypoxia survival.

关键词： adaptive response cellular signal ethylene nitrogen acquisition and utilization

The Clausena lansium genome provides new insights into alkaloid diversity and the evolution of the methyltransferase family

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Journal of Integrative Agriculture 2024年第10期 3537-3553页

作者： Yongzan Wei Yi Wang Fuchu Hu Wei Wang Changbin Wei Bingqiang Xu Liqin Liu Huayang Li Can Wang Hongna Zhang Zhenchang Liang Jianghui Xie state key laboratory of Biological Breeding of Tropical Crops Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences key laboratory of Tropical Fruit Biology (Ministry of Agriculture and Rural Affairs) South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences Sanya Research Academy Chinese Academy of Tropical Agricultural Sciences Beijing key laboratory of Grape Sciences and Enology CAS Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences key laboratory of Genetic Resources Evaluation and utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province) Ministry of Agriculture and Rural Affairs/Institute of Tropical Fruit Trees Hainan Academy of Agricultural Sciences Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Sanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry Hainan University

Wampee(Clausena lansium) is an important evergreen fruit tree native to southern China that has a long history of use for medicinal purposes. Here, a chromosome-level genome of C. lansium was constructed with a genome size of 282.9 Mb and scaffold N50 of 30.75 Mb. The assembled genome contains 48.70% repetitive elements and 24,381 protein-coding genes. Comparative genomic analysis showed that C. lansium diverged from Aurantioideae 15.91–24.95 million years ago. Additionally, some expansive and specific gene families related to methyltransferase activity and S-adenosylmethionine-dependent methyltransferase activity were also *** analysis indicated that N-methyltransferase(NMT) is mainly involved in alkaloid biosynthesis and O-methyltransferase(OMT) participates in the regulation of coumarin accumulation in wampee. This suggested that wampee's richness in alkaloids and coumarins might be due to the gene expansions of NMT and OMT. The tandem repeat event was one of the major reasons for the NMT expansion. Hence, the reference genome of C. lansium will facilitate the identification of some useful medicinal compounds from wampee resources and reveal their biosynthetic pathways.

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Dietary calcium supplementation promotes the accumulation of intramuscular fat

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Journal of Animal Science and Biotechnology 2022年第2期13卷 536-550页

作者： Zhiwang Zhang Tingli Pan Yu Sun Siqi Liu Ziyi Song Haojie Zhang Yixing Li Lei Zhou state key laboratory for conservation and utilization of subtropical agro-bioresources College of Animal Science and TechnologyGuangxi UniversityGuangxi Zhuang Autonomous RegionNanning 530004People’s Republic of China

Background:In the livestock industry,intramuscular fat content is a key factor affecting meat *** studies have shown that dietary calcium supplementation is closely related to lipid ***,few studies have examined the relationship between dietary calcium supplementation and intramuscular fat ***:Here,we used C2C12 cells,C57BL/6 mice(n=8)and three-way cross-breeding pigs(Duroc×Landrace×Large white)(n=10)to study the effect of calcium addition on intramuscular fat *** vitro,we used calcium chloride to adjust the calcium levels in the medium(2 mmol/L or 3 mmol/L).Then we measured various *** vivo,calcium carbonate was used to regulate calcium levels in feeds(Mice:0.5%calcium or 1.2%calcium)(Pigs:0.9%calcium or 1.5%calcium).Then we tested the mice gastrocnemius muscle triglyceride content,pig longissimus dorsi muscle meat quality and ***:In vitro,calcium addition(3 mmol/L)had no significant effect on cell proliferation,but promoted the differentiation of C2C12 cells into slow-twitch *** supplementation increased triglyceride accumulation in C2C12 *** addition increased the number of mitochondria and also increased the calcium level in the mitochondria and reduced the of key enzymes activity involved inβ-oxidation such as acyl-coenzyme A *** mitochondrial calcium level can alleviate lipid accumulation induced by calcium *** addition,calcium addition also reduced the glycolytic capacity and glycolytic conversion rate of C2C12 *** vivo,dietary calcium supplementation(1.2%)promoted the accumulation of triglycerides in the gastrocnemius muscle of *** calcium supplementation(1.5%)had no effect on pig weight,but significantly improved the flesh color of the longissimus dorsi muscle,reduced the backfat thickness and increased intramuscular fat content in ***,calcium addition had no effect on longissimus dorsi pH,electrical conducti

关键词： C2C12 Calcium Intramuscular fat Meat quality Pig

STI PCR: An efficient method for amplification and de novo synthesis of long DNA sequences

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Molecular Plant 2022年第4期15卷 620-629页

作者： Zhe Zhao Xianrong Xie Weizhi Liu Jingjing Huang Jiantao Tan Haixin Yu Wubei Zong Jintao Tang Yanchang Zhao Yang Xue Zhizhan Chu Letian Chen Yao-Guang Liu state key laboratory for conservation and utilization of subtropical agro-bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong laboratory for Lingnan Modern Agriculture Guangzhou 510642China

Despite continuous improvements,it is difficult to efficiently amplify large sequences from complex templates using current PCR ***,we developed a suppression thermo-interlaced(STI)PCR method for the efficient and specific amplification of long DNA sequences from genomes and synthetic DNA *** method uses site-specific primers containing a common 50 tag to generate a stem-loop structure,thereby repressing the amplification of smaller non-specific products through PCR suppression(PS).However,large target products are less affected by PS and show enhanced amplification when the competitive amplification of non-specific products is ***,this method uses nested thermointerlaced cycling with varied temperatures to optimize strand extension of long sequences with an uneven GC *** combination of these two factors in STI PCR produces a multiplier effect,markedly increasing specificity and amplification *** also developed a webtool,calGC,for analyzing the GC distribution of target DNA sequences and selecting suitable thermo-cycling programs for STI *** this method,we stably amplified very long genomic fragments(up to 38 kb)from plants and human and greatly increased the length of de novo DNA synthesis,which has many applications such as cloning,expression,and targeted genomic *** method greatly extends PCR capacity and has great potential for use in biological fields.

关键词： STI PCR PCR suppression thermo-interlaced cycling long genomic fragments de novo DNA synthesis

OsDREB1C,an integrator for photosynthesis,nitrogen use efficiency,and early flowering

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Science China(Life Sciences) 2023年第1期66卷 191-193页

作者： Wei Wang Dong Zhang Chengcai Chu state key laboratory for conservation and utilization of subtropical agro-bioresources College of AgricultureSouth China Agricultural UniversityGuangzhou 510642China Guangdong laboratory for Lingnan Modern Agriculture Guangzhou 510642China

Food security is a major challenge for people around the *** the continuous growth of food supply in the past sixty years,more than 800 million people in the world are still suffering from hunger due to climate change and geographical conflicts(Food and Agriculture Organization of the United Nations,FAO,2020,https://***/hunger/en/).

关键词： sixty spite suffering

Abscisic acid-dependent PMT1 expression regulates salt tolerance by alleviating abscisic acid-mediated reactive oxygen species production in Arabidopsis

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Journal of Integrative Plant Biology 2022年第9期64卷 1803-1820页

作者： Qi Yu He Jian Feng Jin He Qiang Lou Feng Feng Dang Ji Ming Xu Shao Jian Zheng Jian Li Yang state key laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou310058 China state key laboratory of subtropical Silviculture Zhejiang A&F UniversityHangzhou311300 China state key laboratory for conservation and utilization of subtropical agro-bioresources South China Agricultural UniversityGuangzhou510642 China

Phosphocholine(PCho)is an intermediate metabolite of nonplastid plant membranes that is essential for salt ***,how PCho metabolism modulates response to salt stress remains ***,we characterize the role of phosphoethanolamine N-methyltransferase 1(PMT1)in salt stress tolerance in Arabidopsis thaliana using a T-DNA insertional mutant,geneediting alleles,and complemented *** pmt1 mutants showed a severe inhibition of root elongation when exposed to salt stress,but exogenous ChoCl or lecithin rescued this ***1 also displayed altered glycerolipid metabolism under salt stress,suggesting that glycerolipids contribute to salt ***,pmt1 mutants exhibited altered reactive oxygen species(ROS)accumulation and distribution,reduced cell division activity,and disturbed auxin distribution in the primary root compared with wild-type *** show that PMT1 expression is induced by salt stress and relies on the abscisic acid(ABA)signaling pathway,as this induction was abolished in the aba2-1 and pyl112458 ***,ABA aggravated the salt sensitivity of the pmt1 mutants by perturbing ROS distribution in the root *** together,we propose that PMT1 is an important phosphoethanolamine N-methyltransferase participating in root development of primary root elongation under salt stress conditions by balancing ROS production and distribution through ABA signaling.

关键词： abscisic acid(ABA) oxidative stress plasma membrane phosphoethanolamine N-methyltransferase phospholipid metabolism salt stress