Sequence analysis of Erianthus arundinaceus chromosome 1 isolated by flow sorting after genomic in situ hybridization in suspension

作     者：Shan Yang Petr Cápal Jaroslav Dolezel Xueting Li Wang Qian Zhiqiang Wang Kai Zeng Peiting Li Hongkai Zhou Rui Xia Muqing Zhang Zuhu Deng Shan Yang;Petr Cápal;Jaroslav Dole?el;Xueting Li;Wang Qian;Zhiqiang Wang;Kai Zeng;Peiting Li;Hongkai Zhou;Rui Xia;Muqing Zhang;Zuhu Deng

作者机构：National Engineering Research Center for Sugarcane&State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources&Guangxi Key Laboratory of Sugarcane BiologyGuangxi UniversityNanning 530004GuangxiChina Institute of Experimental Botany of the Czech Academy of SciencesCenter of the Region Hanáfor Biotechnological and Agricultural ResearchOlomouc CZ-77900Czech Republic College of Coastal Agricultural SciencesGuangdong Ocean UniversityZhanjiang 524088GuangdongChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第6期

页      面：1746-1754页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：funded by the National Natural Science Foundation of China(31771863) Science and Technology Major Project of the Fujian Province of China(2015NZ0002-2) Special Fund for Scientific and Technological Innovation of the Fujian Agriculture and Forestry University(KFA17168A) Doctoral Students of Fujian Agriculture and Forestry University Going Abroad to Cooperative Research(324-112110082) Key Laboratory of Conservation and Utilization of Subtropical Agricultural Biological Resources(SKLCUSA-a201912) supported by ERDF Project“Plants as a tool for sustainable global development”(CZ.02.1.01/0.0/0.0/16_019/0000827) 

主　　题：Flow cytometry GISHIS Chromosome sorting Genome analysis Sugarcane 

摘      要：Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic ***,limited knowledge of the complex genome of hexaploid *** has impeded the development of required molecular *** complex genomes into single chromosomes can simplify *** flow-cytometric sorting of a single chromosome of *** in a Saccharum-Erianthus introgression line is reported.A novel approach called genomic in situ hybridization in suspension was used to discriminate the alien chromosome from sugarcane chromosomes at the same size.A total of 218,000 *** chromosome 1(EaC1)were sorted to97%purity and amplified DNA was sequenced using Illumina and Pac Bio *** resulting assembly had a 70.93 Mb contig sequence with an N50 of 19.62 kb.A total of 56.69 Mb repeat sequences were predicted,accounting for 79.1%of the chromosome and 2646 genes having a total length of 1.84 Mb that represented 2.59%of the *** these genes,1877(70.9%)genes were functionally *** phylogenetic relationship of *** with other species using the chromosome1 sequence revealed that *** was distantly related to Oryza sativa and Zea mays,followed by Sorghum bicolor,and was closely related to *** and Saccharum *** study provides the first insights into the characteristics of EaC1,and the results will provide tools to support molecular improvement and alien introgression breeding of sugarcane.