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MACF1 deficiency suppresses tooth mineralization through IGF1 mediated crosstalk between odontoblasts and ameloblasts

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Genes & Diseases 2024年第5期11卷 9-12页

作者： Wuxia Qiu Xiao Lin Shaoqing Yang Zhihao Chen Kewen Zhang Chaofei Yang Yu Li Zhiping Miao Xiaoni Deng Xiaohong Duan Airong Qian lab for Bone Metabolism Xi’an Key Laboratory of Special Medicine and Health EngineeringKey Lab for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical UniversityXi’anShaanxi 710072China state key laboratory of Military Stomatology National Clinical Research Center for Oral DiseasesDepartment of Oral BiologyClinic of Oral Rare and Genetic DiseasesSchool of StomatologyThe Fourth Military Medical UniversityXi’anShaanxi 710032China School of Chemical Engineering Sichuan University of Science&EngineeringZigongSichuan 643000China Research&Development Institute of Northwestern Polytechnical University in Shenzhen ShenzhenGuangdong 518063China Puyang Institute of Technology Henan UniversityPuyangHenan 457000China

Tooth mineralization is a ubiquitous and tightly regulated process involving complicated interactions between dental epithelium and *** molecules in tooth mineralization remain poorly *** actin cross-linking factor 1(MACF1)is a spectraplakin protein that plays pivotal roles in the brain,muscle,lung,and bone developmental process.^(1-3) To study the specific functions of MACF1 in bone formation,we established Macf1 conditional knockout mice using the Cre-LoxP system driven by Osxterix promoter(Osx-Cre;Macf1^(f/f)).^(2) Not surprisingly,Osx-Cre;Macf1^(f/f) mice displayed the phenotypes of delayed ossification and decreased bone ***,the OsxCre;Macf1^(f/f) mice unexpectedly showed a white and opaque appearance of incisors,contrary to the normal yellowbrown and transparent *** Osxterix is expressed in dental mesenchyme during tooth development,the abnormal tooth appearance might imply a new function of MACF1 in odontoblasts,or even ***,the present study aimed to investigate the role of MACF1 during tooth development.

关键词： mineralization linking transparent

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Efficient and reproducible folding simulations of the Trp-cage protein with multiscale molecular dynamics

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Chinese Science Bulletin 2008年第11期53卷 1699-1707页

作者： XIA XueFeng ZHANG Song HUANG Bo ZHOU Yun SUN ZhiRong Department of Biology/MOE key laboratory of Bioinforrnatics State Key Laboratory of Biomembrane and Membrane BiotechnologyTsinghua University Beijing 100084 China

Folding simulations are often time-consuming or highly sensitive to the initial conformation of the si-mulation even for mini protein like the Trp-cage. Here, we present a multiscale molecular dynamics method which appears to be both efficient and insensitive to the starting conformation based on the testing results from the Trp-cage protein. In this method the simulated system is simultaneously mod-eled on atoms and coarse-grained particles with incremental coarsening levels. The dynamics of coarse-grained particles are adapted to the recent trajectories of finer-grained particles instead of fixed and parameterized energy functions as used in previous coarse-grained models. In addition, the com-positions of coarse-grained particles are allowed to be updated automatically based on the coherence during its history. Starting from the fully extended conformation and other several different conforma-tions of the Trp-cage protein, our method successfully finds out the native-like conformations of the Trp-cage protein in the largest cluster of the trajectories in all of the eight performed simulations within at most 10 ns simulation time. The results show that approaches based on multiscale modeling are promising for ab initio protein structure prediction.

关键词：蛋白质分子动力学分子结构结构模拟

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Designing the lipid raft marker protein for synaptic vesicles

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High Technology Letters 2009年第1期15卷 101-107页

作者：吕继华 Sui Senfang Department of Biological Sciences ＆ biotechnology State-Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing 100084 P.R. China

Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological ac-tivities such as the neurotransmitter *** studies have been carried out on the function of rafts inthe plasma membranes,whereas little is known about the information of such microdomains in subcellularcompartments especially synaptic vesicles(SVs).In the well-studied plasma membranes,several proteinshave been recognized as raft markers,which are used to label or trace *** the raft marker proteinon SVs has not been identified *** some SV proteins,including VAMP and CPE,have beenfound in raft fractions,they cannot be used as markers due to their low abundance in *** this work,we designed several chimera proteins and tested their characteristics for using as SV raft ***,we detected whether they located in SVs,and then the chimeras exhibiting the better localization in SVswere further examined for their enrichment in raft using detergent treatment and gradient density *** results indicate that one of the chimeric proteins is primarily located in SVs and distributedin raft microdomains,which strongly suggests that it could be served as a raft marker for SVs.

关键词： lipid rafts synaptic vesicles marker protein

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Adipose-derived mesenchymal stem cell transplantation promotes adult neurogenesis in the brains of Alzheimer's disease mice

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Neural Regeneration Research 2014年第8期9卷 798-805页

作者： Yufang Yan Tuo Ma Kai Gong Qiang Ao Xiufang Zhang Yao Gong state key laboratory of biomembrane and membrane biotechnology School of Life SciencesTsinghua University Institute of Neurological Disorders Yuquan HospitalTsinghua University

In the present study, we transplanted adipose-derived mesenchymal stem cells into the hippo-campi of APP/PS1 transgenic Alzheimer＇s disease model mice. Immunofluorescence staining revealed that the number of newly generated （BrdU＋） cells in the subgranular zone of the dentate gyrus in the hippocampus was signiifcantly higher in Alzheimer＇s disease mice after adipose-de-rived mesenchymal stem cell transplantation, and there was also a significant increase in the number of BrdU＋/DCX＋neuroblasts in these animals. Adipose-derived mesenchymal stem cell transplantation enhanced neurogenic activity in the subventricular zone as well. Furthermore, adipose-derived mesenchymal stem cell transplantation reduced oxidative stress and alleviated cognitive impairment in the mice. Based on these ifndings, we propose that adipose-derived mes-enchymal stem cell transplantation enhances endogenous neurogenesis in both the subgranular and subventricular zones in APP/PS1 transgenic Alzheimer＇s disease mice, thereby facilitating functional recovery.

关键词： nerve regeneration stem cells Alzheimer's disease adipose-derived mesenchymal stemcells cell transplantation cognitive impairment oxidative stress neurogenesis 863 Program neuralregeneration

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Tunneling nanotubes between rat primary astrocytes and C6 glioma cells alter proliferation potential of glioma cells

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Neuroscience Bulletin 2015年第3期31卷 371-378页

作者： Lei Zhang Yan Zhang state key laboratory of biomembrane and membrane biotechnology College of Life Sciences PKU-IDG/Mc Govern Institute for Brain Research Peking University

The tunneling nanotube （TNT） is a newly discovered, long and thin tubular structure between cells. In this study, we established a co-culture system for rat primary astrocytes and C6 glioma cells and found that TNTs formed between them. Most of the TNTs initiated from astrocytes towards C6 glioma cells. The formation of TNTs depended on p53. In addition, hydrogen peroxide increased the number of TNTs in the co-culture system. Established TNTs reduced the proliferation of C6 glioma cells. Our data suggest that TNTs between astrocytes and glioma cells facilitate substance transfer and therefore alter the properties, including the proliferation potential, of glioma cells.

关键词： tunneling nanotube astrocyte glioma proliferation p53

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SP-141 targets Trs85 to inhibit rice blast fungus infection and functions as a potential broadspectrum antifungal agent

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Plant Communications 2024年第2期5卷 169-184页

作者： Xi-Yu Wu Bo Dong Xue-Ming Zhu Ying-Ying Cai Lin Li Jian-Ping Lu Bin Yu Jing-Li Cheng Fei Xu Jian-Dong Bao Ying Wang Xiao-Hong Liu Fu-Cheng Lin state key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of BiotechnologyZhejiang UniversityHangzhou 310058Zhejiang ProvinceChina state key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhou 310058Zhejiang ProvinceChina Department of Pharmacology and Nutritional Science College of MedicineThe University of KentuckyLexingtonKY 40506USA Markey Cancer Center College of MedicineThe University of KentuckyLexingtonKY 40506USA College of Life Sciences Zhejiang UniversityHangzhou 310058Zhejiang ProvinceChina Institute of Pesticide and Environmental Toxicology Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and InsectsZhejiang UniversityHangzhou 310058Zhejiang ProvinceChina Institute of Edible Fungi Shanghai Academy of Agricultural SciencesShanghai 201106ShanghaiChina

Rice blast is a devastating disease worldwide,threatening rice production and food *** blast fun-gus Magnaporthe oryzae invades the host via the appressorium,a specialized pressure-generating struc-ture that generates enormous turgor pressure to penetrate the host ***,owing to ongoing evolution of fungicide resistance,it is vitally important to identify new targets and ***,we show that Trs85,a subunit of the transport protein particle III complex,is essential for appressorium-mediated infection in *** explain how Trs85 regulates autophagy through Ypt1(a small guano-sine triphosphatase protein)in *** then identify a key conserved amphipathic a helix within Trs85 that is associated with pathogenicity of *** computer-aided screening,we identify a lead compound,SP-141,that affects autophagy and the Trs85–Ypt1 ***-141 demonstrates a substantial capacity to effectively inhibit infection caused by the rice blast fungus while also exhibiting wide-ranging potential as an antifungal agent with broad-spectrum *** together,our data show that Trs85 is a potential new target and that SP-141 has potential for the control of rice *** thus provide a novel strategy that may help in thefight against rice blast.

关键词： Magnaporthe oryzae rice blast TRAPPIII complex trs85 fungicide

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Effects of solid-state fermentation product of yeast supplementation on liver and intestinal health,and resistance of common carp(Cyprinus carpio)against spring viraemia carp virus

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Animal Nutrition 2024年第3期18卷 408-418页

作者： Mengxin Wang Dongmei Xia Lijuan Yu Qiang Hao Mingxu Xie Qingshuang Zhang Yajie Zhao Delong Meng Yalin Yang Chao Ran Tsegay Teame Zhen Zhang Zhigang Zhou China-Norway Joint lab on Fish Gut Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Hubei key laboratory of Animal Nutrition and Feed Science Wuhan Polytechnic UniversityWuhan 430000China key laboratory for Feed biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Tigray Agricultural Research Institute Mekelle Agricultural Research CenterMekelleTigray 251Ethiopia

This study aimed to investigate the effects of solid-state fermentation products of yeast(SFPY)on liver and intestinal health and disease resistance of common carp(Cyprinus carpio).A total of 200 common carp with an initial average weight of 2.55±0.004 g were divided into 5 groups(4 replications per group and 10 fish per replication),and were fed with one of five diets,including a control diet and 4 diets supplemented with 2‰(Y2),3‰(Y3),4‰(Y4),or 5‰(Y5)SFPY,respectively,for 8 *** indicated that,the addition of SFPY to the diet of common carp did not affect the growth performance or survival rate of fish(P=0.253).Interestingly,with the addition of SFPY,the triacylglycerol(TAG)content of the liver presented a linear decreasing tendency(P=0.004),with significantly decreased in Y4 and Y5 groups(P=0.035)compared with *** lipopolysaccharide(LPS)content and diamine oxidase(DAO)activity presented a negative linear relationship with the addition of SFPY(P=0.015,P=0.030),while serum lipopolysaccharide binding protein(LBP)content first decreased and then increased(P<0.001).The total antioxidant capacity(T-AOC)in the intestine of fish increased continuously with increasing SFPY supplementation(P=0.026),reaching the highest level in Y5 *** villus height in all experimental groups were significantly higher than that in the control group(P<0.001).Furthermore,compared to the control,adding 3‰SFPY to the control diet of common carp significantly increased the relative abundance of Fusobacteria(P=0.018)and decreased that of Proteobacteria(P=0.039)at phylum level,and increased the relative abundance of Cetobacterium(P=0.018)and decreased that of Shewanella(P=0.013)at genus *** with the control,the relative mRNA expression level of spring viraemia of carp virus N protein(SVCV-n)in the kidney was lower than that of the control group without significance and bottomed out in Y4 group(P=0.138).In conclusion,dietary SFPY enhanced the SVCV resistance capa

关键词： Yeast Solid-state fermentation product Probiotic Immunity Gut microbiota Common carp

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Yin Yang 1(YY1) synergizes with Smad7 to inhibit TGF-β signaling in the nucleus

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Science China(Life Sciences) 2014年第1期57卷 128-136页

作者： YAN XiaoHua PAN Jun XIONG WanWan CHENG MinZhang SUN YingYuan ZHANG SuPing CHEN YeGuang state key laboratory of biomembrane and membrane biotechnology Tsinghua-Peking Center for Life Sciences School of Life SciencesTsinghua University

As a prototype of the TGF-β superfamily cytokines, TGF-β is well known for its diverse roles in embryogenesis and adult tis- sue homeostasis. TGF-β evokes cellular responses by signaling mainly through cell membrane receptors and transcription fac- tor R-Smads and Co-Smad （Smad4）, while an inhibitory Smad, Smad7, acts as a critical negative regulator of TGF-β signaling. Smad7 antagonizes TGF-β signaling by regulating the stability or activity of the receptors or blocking the DNA binding of the functional R-Smad-Smad4 complex in the nucleus. However, the function of Smad7 in the nucleus is not fully understood. Yin Yang 1 （YY1） is a ubiquitously expressed transcription factor with multiple functions. It has been reported that YY1 can inhib- it Smad-dependent transcriptional responses and TGF-β/BMP-induced cell differentiation independently of its DNA binding ability. In this study, we found that Smad7 interacts with YY1 and the interaction is attenuated by TGF-β signaling. Reporter assays and target gene expression analyses revealed that Smad7 and YY1 act in concert to inhibit TGF-β-induced transcription in the nucleus. Furthermore, Smad7 could enhance the interaction of YY1 with the histone deacetylase HDAC1. Consistently, YY 1 and HDAC 1 augmented the transcription repression activity of Smad7 in Gal4-1uciferase reporter analysis. Therefore, our findings define a novel mechanism of Smad7 and YY1 to antagonize TGF-β signaling.

关键词： TGF-β Smad7 YY1 HDAC1 signaling regulation transcriptional co-repressor

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Electrophysiological research of synapse formation from beginning to maturity

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Chinese Science Bulletin 1998年第1期43卷 55-59页

作者： XIE Zuoping and ZHENG Yufang state key laboratory of biomembrane and membrane Biotechnique Department of Biological Sciences and biotechnology, Tsinghua University, Beijing 100084, China state key laboratory of biomembrane and membrane Biotechnique Tsinghua University Beijing China Department of Biological Sciences and biotechnology Tsinghua University Beijing China

The development of synapses is an important question in neurobiology. We employ patchclamp technique to record MEPCs and EPCs of myoballs in Xenopus cell culture. The rate of EPC/MEPC is related with synapses maturation. Accordingly, we define the mature synapses as those whose rate of presynaptic membrane’s EPC to MEPC is more than 8. The process of maturation needs about 2-5 h after neurites contact with myoballs. We divide the development of synapses into three stages: young, developing and mature. The neurotransmitters stack gradually and increase by hundreds of times along with young synapses growing into maturity. So the process of synapses maturation should include presynaptic membrane specialization, postsynaptic membrane specialization and neurotransmitters stack.

关键词： definition of mature synapse α latrotoxin (α LTX) neurotransmitter stack.

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High efficient gene transfer introduced by low-amplitude, low-frequency alternating electric field

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Chinese Science Bulletin 1996年第6期41卷 516-519页

作者：陈刚蔡国平赵南明 state key laboratory of biomembrane and membrane biotechnology School of Life Science and EngineeringTsinghua University Beijing 100084 China

Recently, gene transfer by electric pulses (electroporation) has become one of the mostpopular and effective methods for introducing foreign genes into bacterial, plant and ani-mal cells. As a physical method, electric field has many advantages, such as simple opera-tion, high transfection efficiency and few biological side-effects. The mechanism ofelectroporation is based on high voltage DC electric pulses able to cause cell membranepermeability, followed by the foreign gene diffusing into the host cells. This kind

关键词： alternating electric field HeLa cell chlamydomonas gene transfer.

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