检索结果-南通市图书馆

RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer

Cancer Biology & Medicine 2021年第1期18卷 120-138页

作者： Guosheng Lyu Peng Su Xiaohe Hao Shiming Chen Shuai Ren Zixiao Zhao Yaoqin Gong Qiao Liu Changshun Shao Key Laboratory of Experimental Teratology Ministry of EducationDepartment of Molecular Medicine and GeneticsSchool of Basic Medical SciencesShandong UniversityJinan 250012China Department of Pathology Qilu Hospital of Shandong UniversityJinan 250012China State Key Laboratory of radiation Medicine and Protection Institutes for Translational MedicineSoochow UniversitySuzhou 215123China

Objective:RECQL4(a member of the RECQ helicase family)upregulation has been reported to be associated with tumor progression in several ***,whether RECQL4 sustains esophageal squamous cell carcinoma(ESCC)has not been *** this study,we determined the functional role for RECQL4 in ESCC ***:RECQL4 expression in clinical samples of ESCC was examined by *** proliferation,cellular senescence,the epithelial-mesenchymal transition(EMT),DNA damage,and reactive oxygen species in ESCC cell lines with RECQL4 depletion or overexpression were *** levels of proteins involved in the DNA damage response(DDR),cell cycle progression,survival,and the EMT were determined by western blot ***:RECQL4 was highly expressed in tumor tissues when compared to adjacent non-tumor tissues in ESCC(P<0.001)and positively correlated with poor differentiation(P=0.011),enhanced invasion(P=0.033),and metastasis(P=0.048).RECQL4 was positively associated with proliferation and migration in ESCC *** of RECQL4 also inhibited growth of tumor xenografts in ***4 depletion induced G0/G1 phase arrest and cellular ***,the levels of DNA damage and reactive oxygen species were increased when RECQL4 was ***,as measured by the activation of ATM,ATR,CHK1,and CHK2,was ***4 was also shown to promote the activation of AKT,ERK,and NF-k B in ESCC ***:The results indicated that RECQL4 was highly expressed in ESCC and played critical roles in the regulation of DDR,redox homeostasis,and cell survival.

关键词： ESCC RECQL4 senescence redox DNA damage response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

引用

World Journal of Stem Cells 2023年第7期15卷 713-733页

作者： Li-Jun Wu Wei Lin Jian-Jiang Liu Wei-Xin Chen Wen-Jun He Yuan Shi Xiao Liu Ke Li Department of Plastic and Aesthetic Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina Department of Burn and Plastic Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND Current evidence shows that human induced pluripotent stem cells(hiPSCs)can effectively differentiate into keratinocytes(KCs),but its effect on skin burn healing has not been *** To observe the effects of hiPSCs-derived KCs transplantation on skin burn healing in mice and to preliminarily reveal the underlying *** An analysis of differentially expressed genes in burn wounds based on GEO datasets GSE140926,and GSE27186 was established.A differentiation medium containing retinoic acid and bone morphogenetic protein 4 was applied to induce hiPSCs to differentiate into *** expression of KCs marker proteins was detected using immunofluorescence staining.A model of a C57BL/6 mouse with deep cutaneous second-degree burn was created,and then phosphate buffered saline(PBS),hiPSCs-KCs,or hiPSCs-KCs with knockdown of COL7A1 were injected around the wound *** wound healing,re-epithelialization,engraftment of hiPSCs-KCs into wounds,proinflammatory factor level,and the NF-κB pathway proteins were assessed by hematoxylin-eosin staining,carboxifluorescein diacetate succinimidyl ester(CFSE)fluorescence staining,enzyme linked immunosorbent assay,and western blotting on days 3,7,and 14 after the injection,***,the effects of COL7A1 knockdown on the proliferation and migration of hiPSCs-KCs were confirmed by immunohistochemistry,EdU,Transwell,and damage repair *** HiPSCs-KCs could express the hallmark proteins of ***7A1 was down-regulated in burn wound tissues and highly expressed in *** of hiPSCs-KCs into mice with burn wounds resulted in a significant decrease in wound area,an increase in wound re-epithelialization,a decrease in proinflammatory factors content,and an inhibition of NF-κB pathway activation compared to the PBS *** in vitro assay showed that COL7A1 knockdown could rescue the inhibition of hiPSCs-KCs proliferation and migration,providing further evidence that COL7A

关键词： Induced pluripotent stem cell Keratinocytes Cell transplantation Burn wound healing COL7A1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The crystal structure of Zika virus helicase:basis for antiviral drug design

引用

Protein & Cell 2016年第6期7卷 450-454页

作者： Hongliang Tian Xiaoyun Ji Xiaoyun Yang Wei Xie Kailin Yang Cheng Chen Chen Wu Heng Chi Zhongyu Mu Zefang Wang Haitao Yang School of Life Sciences Tianjin University Tianjin International joint Academy of Biotechnology and Medicine The State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing University Cleveland Clinic Lerner College of Medicine of Case western Reserve university

Dear Editor The genus of Flavivirus contains important human pathogens,including dengue(DENV),yellow fever(YFV),West Nile(WNV),Japanese encephalitis(JEV),and tick-borne encephalitis(TBEV)viruses,which cause a number of serious human diseases throughout the world(Pierson TC,2013).Zika virus(ZIKV)is also an arthropod-borne flavivirus,which was initially isolated in 1947 from a febrile sentinel rhesus monkey in the Zika forest in Entebbe,*** is

关键词： Japanese Encephalitis Helicase Domain ZIKV Infection Murray Valley Encephalitis Virus Shanghai synchrotron radiation Facility

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

PIG3 downregulation enhances the radiosensitivity of NSCLC cells by promoting G_(2)/M cell cycle arrest and apoptosis

引用

radiation Medicine and Protection 2023年第1期4卷 19-25页

作者： Hao Zhou Linfeng Tang Zeng-Fu Shang Ping-Kun Zhou Ming Li State Key Laboratory of radiation Medicine and Protection School of Radiation Medicine and ProtectionMedical College of Soochow UniversitySuzhou 215123China Collaborative Innovation Center of radiation Medicine of Jiangsu Higher Education Institutions Soochow UniversitySuzhou 215123China Department of radiation Toxicology and Oncology Beijing Key Laboratory for RadiobiologyBeijing Institute of Radiation MedicineBeijing 100850China

Objective:To investigate the mechanism of p53-induced gene 3(PIG3)-regulation of radioresistance in human non-small cell lung cancer(NSCLC)cells,in order to explore new biomarkers and therapeutic targets to combat radioresistance and improve the 5-year survival ***:The PIG3 gene was knocked down in A549 cells using siRNA,and was overexpressed in H1299 cells using a PIG3 expression *** confirming PIG3 knockdown and overexpression through the western blot analysis,the radiosensitivity,DNA damage,cell cycle distribution,and apoptosis in these cells were analyzed using colony formation assay,immunofluorescence staining forγH2AX,and flow cytometry,***:PIG3 silencing markedly increased the radiosensitivity of NSCLC cells,with radiosensitization ratios of 1.12 and *** with the corresponding negative control,PIG3 knockdown significantly enhanced G2/M phase arrest(siNC:26.12±2.50,siPIG3#1:34.98±4.19,siPIG3#2:37.79±3.53,Pradiation-induced apoptosis(siNC:14.61±1.85,siPIG3#1:17.26±1.14,siPIG3#2:20.70±2.04,Pradiation(P<0.05).Conversely,PIG3 overexpression markedly decreased the radiosensitivity of NSCLC cells,as evidenced by the reduction of G2/M phase arrest(NC:33.18±2.11 ***3:24.21±3.09,P<0.05)and apoptosis(NC:15.49±0.56 ***3:12.79±0.29,P<0.05),and increased DNA damage(P<0.05).Conclusions:PIG3 downregulation increases the radiosensitivity of NSCLC cells,and PIG3-upregulation leads to the progression in ***,PIG3 is a potential target for radiotherapy for NSCLC.

关键词： Radioresistance PIG3 Non-small cell lung cancer Cell cycle Apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Orchestration of the balance in liver metabolism by c-Fos

Orchestration of the balance in liver metabolism by c-Fos

引用

中国毒理学会第十次全国毒理学大会

作者： Zhisen Zhang Lei Chang State Key Laboratory of radiation Medicine and Protection School of Radiation Medicine and Protection (SRMP) of Soochow University

Non-alcoholic fatty liver disease(NAFLD) is a dietary disease associate with obesity and diabetes mellitus, which can develop into nonalcoholic steatohepatitis(NASH) without effective restrictions. However, there is no therapies available. People who favor high calories food and sugar water are more susceptible to NAFLD, which may due to the disequilibrium of lipid accumulation and metabolism. Many HCC patients express c-Fos, a component of the AP-1 transcription factor. Here, we found that when treated with high fat diet, liver specific c-Fos knock out could alleviate the body weight gain and lipid aggregation through inhibiting the sterol regulatory element-binding protein-1c(SREBP-1c) cleavage. SREBP-1c is a core transcription factor that regulate the lipid synthesis, and the cleaved SREBP-1c could translocate into nucleus to orchestrate the de novo lipogenesis(DNL) related genes such as Fasn, Scd-1. Our data identifies the component of AP-1 transcription factor, c-Fos as a key regulator upstream of lipid synthesis and provide a potential target for the NAFLD treatment. METHODS Animals: The c-Fosfl/fl, AlbCreERT2mice were maintained on a C57BL/6J background, and crossed to generate the c-FosΔLimice. Mice were housed in a specific pathogen-free facility, with normal or high fat diet(60% kCal/fat HFD,D12492, research Diets) and water ad libitum, in a 12 hr light/12 hr dark cycle. If not indicated specifically, 6-8 weeks male mice was fed with HFD. HISTOLOGY H&E staining were performed using standard procedures. qRT-PCR/Immunoblot Analysis: qRT-PCR was performed on Life Technologies ViiA 7 with SYBR mix. Relative expression levels were calculated using the ΔCtmethod. All the data were normalized to 36b4, a house keeping gene. western Blot analysis was performed with standard protocols and following antibodies: β-Actin, Srebp1. CONCLUSION Liver specific knock out of c-Fos could prevent mice from non-alcoholic fatty liver disease through downregulating the cleavage of

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Copper single-atom catalysts with photothermal performance and enhanced nanozyme activity for bacteria-infected wound therapy

引用

Bioactive Materials 2021年第12期6卷 4389-4401页

作者： Xianwen Wang Qianqian Shi Zhengbao Zha Dongdong Zhu Lirong Zheng Luoxiang Shi Xianwen Wei Lian Lian Konglin Wu Liang Cheng Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-based Functional Materials and DevicesSoochow UniversitySuzhou215123PR China School of Food and Biological Engineering Hefei University of TechnologyHefei230009PR China Institute of Clean Energy and Advanced Nanocatalysis School of Chemistry and Chemical EngineeringAnhui University of TechnologyMaanshan243002PR China Beijing synchrotron radiation Facility(NSRF) Institute of High Energy PhysicsChinese Academy of ScienceBeijing100049China Department of Oncology Suzhou Xiangcheng People’s HospitalSuzhou215131China

Nanozymes have become a new generation of antibiotics with exciting broad-spectrum antibacterial properties and negligible biological ***,their inherent low catalytic activity limits their antibacterial ***,Cu single-atom sites/N doped porous carbon(Cu SASs/NPC)is successfully constructed for photothermal-catalytic antibacterial treatment by a pyrolysis-etching-adsorption-pyrolysis(PEAP)*** SASs/NPC have stronger peroxidase-like catalytic activity,glutathione(GSH)-depleting function,and photothermal property compared with non-Cu-doped NPC,indicating that Cu doping significantly improves the catalytic performance of *** SASs/NPC can effectively induce peroxidase-like activity in the presence of H2O2,thereby generating a large amount of hydroxyl radicals(•OH),which have a certain killing effect on bacteria and make bacteria more susceptible to *** introduction of near-infrared(NIR)light can generate hyperthermia to fight bacteria,and enhance the peroxidase-like catalytic activity,thereby generating additional•OH to destroy ***,Cu SASs/NPC can act as GSH peroxidase(GSH-Px)-like nanozymes,which can deplete GSH in bacteria,thereby significantly improving the sterilization ***-catalytic synergistic antibacterial strategy produces almost 100%antibacterial efficiency against Escherichia coli(***)and methicillin-resistant Staphylococcus aureus(MRSA).In vivo experiments show a better PTT-catalytic synergistic therapeutic performance on MRSA-infected mouse ***,our work highlights the wide antibacterial and anti-infective bio-applications of Cu single-atom-containing catalysts.

关键词： Copper single-atom catalysts Nanozymes Photothermal therapy Antibacterial Catalytic therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cooperative interactions among CTA^＋, Br^-and Ag^＋ during seeded growth of gold nanorods

引用

Nano research 2017年第6期10卷 2146-2155页

作者： Yong Xu Lei Chen Xingchen Ye Xuchun Wang Jiaqi Yu Yang Zhao Muhan Cao Zhouhui Xia Baoquan Sun Qiao Zhang Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices Institute of Functional Nano and Soft Materials (FUNSOM) Soochow University Suzhou 215123 China Department of Chemistry University of California Berkeley Berkeley CA 94720 USA

We have carried out a comprehensive study on the formation mechanism of Au nanorods （AuNRs） in binary surfactant mixtures composed of quaternary ammonium halide and sodium oleate （NaOL）. We identify the cetyltrimethyl ammonium （CTA）-Br-Ag^＋ complex as the key ingredient in directing the anisotropic growth of AuNRs. Based on the improved understanding of the cooperative interactions among CTA^＋, Br^- and Ag^＋, we further demonstrate that AgBr, which is readily solubilized by the cetyltrimethyl ammonium bromide （CTAB） or cetyltrimethyl ammonium chloride （CTAC） micelles, can be employed as the combined source of Ag^＋ and Br^- for the preparation of AuNRs. The growth of high-quality AuNRs can be completed within 15 min under extremely low bromide content （0.1 mM）.

关键词： gold nanorods anisotropic nanostructure surface plasmon resonance seeded growth cetyltrimethyl ammoniumbromide (CTAB)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Self-Decoration of PtNi Alloy Nanoparticles on Multiwalled Carbon Nanotubes for Highly Efficient Methanol Electro-Oxidation

引用

Nano-Micro Letters 2016年第4期8卷 371-380页

作者： Yu-Yan Zhou Chang-Hai Liu Jie Liu Xin-Lei Cai Ying Lu Hui Zhang Xu-Hui Sun Sui-Dong Wang Institute of Functional Nano & Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials & DevicesSoochow University School of Materials Science & Engineering Jiangsu Collaborative Innovation Center of Photovoltaic Science and EngineeringChangzhou University

A simple one-pot method was developed to prepare Pt Ni alloy nanoparticles,which can be self-decorated on multiwalled carbon nanotubes in [BMIm][BF4] ionic *** nanohybrids are targeting stable nanocatalysts for fuel cell *** sizes of the supported Pt Ni nanoparticles are uniform and as small as 1–2 ***-to-Ni ratio was controllable by simply selecting a Pt Ni alloy *** alloy nanoparticles with Pt-to-Ni ratio of 1:1 show high catalytic activity and stability for methanol *** performance is much higher compared with those of both Pt-only nanoparticles and commercial Pt/C *** electronic structure characterization on the Pt Ni nanoparticles demonstrates that the electrons are transferred from Ni to Pt,which can suppress the CO poisoning effect.

关键词： PtNi nanoparticles Multiwalled carbon nanotubes Methanol electro-oxidation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synergistic Effects in CNTs-PdAu/Pt Trimetallic Nanoparticles with High Electrocatalytic Activity and Stability

引用

Nano-Micro Letters 2017年第4期9卷 129-138页

作者： Xin-Lei Cai Chang-Hai Liu Jie Liu Ying Lu Ya-Nan Zhong Kai-Qi Nie Jian-Long Xu Xu Gao Xu-Hui Sun Sui-Dong Wang Institute of Functional Nano and Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices Soochow University School of Materials Science and Engineering Jiangsu Collaborative Innovation Center of Photovoltaic Science and Engineering Changzhou University

We present a straightforward physical approach for synthesizing multiwalled carbon nanotubes(CNTs)-Pd Au/Pt trimetallic nanoparticles(NPs), which allows predesign and control of the metal compositional ratio by simply adjusting the sputtering targets and conditions. The small-sized CNTs-Pd Au/Pt NPs(~3 nm, Pd/Au/Pt ratio of 3:1:2) act as nanocatalysts for the methanol oxidationreaction(MOR), showing excellent performance with electrocatalytic peak current of 4.4 A mg^(-1) Pt and high stability over 7000 s. The electrocatalytic activity and stability of the Pd Au/Pt trimetallic NPs are much superior to those of the corresponding Pd/Pt and Au/Pt bimetallic NPs,as well as a commercial Pt/C catalyst. Systematic investigation of the microscopic, crystalline, and electronic structure of the Pd Au/Pt NPs reveals alloying and charge redistribution in the Pd Au/Pt NPs, which are responsible for the promotion of the electrocatalytic performance.

关键词： CNTs PdAu/Pt Trimetallic nanoparticles Methanol oxidation reaction Electrocatalytic activity Synergistic effects

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Controlled 2D growth of organic semiconductor crystals by suppressing “coffee-ring” effect

引用

Nano research 2020年第9期13卷 2478-2484页

作者： Wei Wang Bei Lu Wei Deng Xiujuan Zhang Zhengjun Lu Di Wu Jiansheng Jie Xiaohong Zhang Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123China Key Laboratory of Materials Physics of Ministry of Education School of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China

Owing to enhanced charge transport efficiency arising from the ultrathin nature,two-dimensional(2D)organic semiconductor single crystals(OSSCs)are emerging as a fascinating platform for high-performance organic field-effect transistors(OFETs).However,ucoffee-ring"effect induced by an evaporation-induced convective flow near the contact line hinders the large-area growth of 2D OSSCs through a solution ***,we develop a new strategy of suppressing the"coffee-ring"effect by using an organic semiconductor:polymer blend *** the high-viscosity polymer in the organic solution,the evaporation-induced flow is remarkably weakened,ensuring the uniform molecule spreading for the 2D growth of the *** an example,wafer-scale growth of crystalline film consisting of few-layered 2,7-didecylbenzothienobenzothiophene(C10-BTBT)crystals was successfully accomplished via blade *** based on the crystalline film exhibited a maximum hole mobility up to 12.6 cm^2·V^-1·s^-1,along with an average hole mobility as high as 8.2 cm^2·V^-1·s^-*** work provides a promising strategy for the large-area growth of 2D OSSCs toward high-performance organic electronics.

关键词： two-dimensional(2D)organic semiconductor single crystals two-dimensional(2D)growth mode coffee-ring effect organic field-effect transistors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：