RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer

作     者：Guosheng Lyu Peng Su Xiaohe Hao Shiming Chen Shuai Ren Zixiao Zhao Yaoqin Gong Qiao Liu Changshun Shao Guosheng Lyu;Peng Su;Xiaohe Hao;Shiming Chen;Shuai Ren;Zixiao Zhao;Yaoqin Gong;Qiao Liu;Changshun Shao

作者机构：Key Laboratory of Experimental TeratologyMinistry of EducationDepartment of Molecular Medicine and GeneticsSchool of Basic Medical SciencesShandong UniversityJinan 250012China Department of PathologyQilu Hospital of Shandong UniversityJinan 250012China State Key Laboratory of Radiation Medicine and ProtectionInstitutes for Translational MedicineSoochow UniversitySuzhou 215123China 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2021年第18卷第1期

页      面：120-138页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by National Natural Science Foundation of China(Grant Nos.81572785,31771260,and 81201750) a Ministry of Science and Technology(Grant No.2011CB966200) the Excellent Young and Mid-Career Scientist Award of Shandong Province(Grant No.BS2013YY023) the Key Research Project of Shandong Province(Grant No.2016GSF201072) the Project of State Key Laboratory of Radiation Medicine and Protection,Soochow University(Grant No.GZN1201804) 

主　　题：ESCC RECQL4 senescence redox DNA damage response 

摘      要：Objective:RECQL4(a member of the RECQ helicase family)upregulation has been reported to be associated with tumor progression in several ***,whether RECQL4 sustains esophageal squamous cell carcinoma(ESCC)has not been *** this study,we determined the functional role for RECQL4 in ESCC ***:RECQL4 expression in clinical samples of ESCC was examined by *** proliferation,cellular senescence,the epithelial-mesenchymal transition(EMT),DNA damage,and reactive oxygen species in ESCC cell lines with RECQL4 depletion or overexpression were *** levels of proteins involved in the DNA damage response(DDR),cell cycle progression,survival,and the EMT were determined by Western blot ***:RECQL4 was highly expressed in tumor tissues when compared to adjacent non-tumor tissues in ESCC(P0.001)and positively correlated with poor differentiation(P=0.011),enhanced invasion(P=0.033),and metastasis(P=0.048).RECQL4 was positively associated with proliferation and migration in ESCC *** of RECQL4 also inhibited growth of tumor xenografts in ***4 depletion induced G0/G1 phase arrest and cellular ***,the levels of DNA damage and reactive oxygen species were increased when RECQL4 was ***,as measured by the activation of ATM,ATR,CHK1,and CHK2,was ***4 was also shown to promote the activation of AKT,ERK,and NF-k B in ESCC ***:The results indicated that RECQL4 was highly expressed in ESCC and played critical roles in the regulation of DDR,redox homeostasis,and cell survival.