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Polypeptide from Moschus Suppresses Lipopolysaccharide-Induced Inflammation by Inhibiting NF-κB-ROS/NLRP3 Pathway

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Chinese Journal of Integrative Medicine 2023年第10期29卷 895-904页

作者： YI Jing LI Li YIN Zhu-jun QUAN Yun-yun TAN Rui-rong CHEN Shi-long LANG Ji-rui LI Jiao ZENG Jin LI Yong SUN Zi-jian ZHAO Jun-ning Department of Pharmaco.ogy Southwest Medical UniversityLuzhouSichuan Province646000China sichuan Institute for Translational Chinese Medicine Translational Chinese Medicine Key Laboratory of Sichuan ProvinceSichuan Academy of Chinese Medicine SciencesChengdu610000China Hunan Provincial Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations College of PharmacyChangsha Medical UniversityChangsha410219China sichuan Fengchun Pharmaceutical co. Ltd.DeyangSichuan Province618100China sichuan Ant Reco.mendation biotechnology co. Ltd.Chengdu610000China

Objective To examine the anti-inflammatory effects and potential mechanisms of polypeptide from Moschus(PPM)in lipopolysaccharide(LPS)-induced THP-1 macrophages and BALB/c *** The polypeptide was extracted from Moschus and analyzed by high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).Subsequently,LPS was used to induce inflammation in THP-1 macrophages and BALB/c *** LPS-treated or untreated THP-1 macrophages,cell viability was observed by cell co.nting kit 8 and lactate dehydrogenase release assays;the proinflammatory cytokines and reactive oxygen species(ROS)were measured by enzyme-linked immunosorbent assay and flow cytometry,respectively;and protein and mRNA levels were measured by Western blot and real-time quantitative polymerase chain reaction(qRT-PCR),*** LPS-induced BALB/c mice,the proinflammatory cytokines were measured,and lung histology and cytokines were observed by hematoxylin and eosin(HE)and immunohistochemical(IHC)staining,*** The SDS-PAGE results suggested that the molecular weight of purified PPM was in the range of 10–26 *** vitro,PPM reduced the production of interleukin 1β(IL-1β),IL-18,tumor necrosis factorα(TNF-α),IL-6 and ROS in LPS-induced THP-1 macrophages(Pco.taining 3(NLRP3)inflammasome pathway by reducing protein expression of phospho-NF-κB p65,phospho-inhibitors of NF-κB(IκBs)kinaseα/β(IKKα/β),TXNIP,NLRP3,apoptosis-associated speck-like protein co.taining a caspase recruitment domain(ASC),and pro-caspase-1(P<0.05 or P<0.01).In addition,qRT-PCR revealed the inhibitory effects of PPM on the mRNA levels of TXNIP,NLRP3,ASC,and caspase-1(P<0.05 or P<0.01).Furthermore,in LPS-induced BALB/c mice,PPM reduced TNF-αand IL

关键词： Moschus polypeptide inflammation NLRP3 inflammasome thioredoxin interacting protein nuclear factorκB Chinese medicine

Patient-derived non-small cell lung cancer xenograft mirrors co.plex tumor heterogeneity

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Cancer Biology & Medicine 2021年第1期18卷 184-198页

作者： Xuanming Chen Cheng Shen Zhe Wei Rui Zhang Yongsheng Wang Lili Jiang Ke Chen Shuang Qiu Yuanli Zhang Ting Zhang Bin Chen Yanjun Xu Qiyi Feng Jinxing Huang Zhihui Zhong Hongxia Li Guowei Che Kai Xiao National Chengdu Center for Safety Evaluation of Drugs and National Clinical Research Center for Geriatrics West China HospitalSichuan UniversityChengdu 610000China sichuan kangcheng biotechnology co. Ltd.Chengdu 610000China Department of Thoracic Surgery West China HospitalSichuan UniversityChengdu 610000China GCP Center West China HospitalSichuan UniversityChengdu 610000China Department of Pathology West China HospitalSichuan UniversityChengdu 610000China Laboratory of Nonhuman Primate Disease Modeling Research State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610000China Center for Infectious Diseases West China HospitalSichuan UniversityChengdu 610000China

Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their co.sistency with primary tumors in phenotypic,genetic,and pharmaco.ynamic heterogeneity has not been *** study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it co.ld preserve the heterogeneity within and between tumors in ***:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID *** on the successful establishment of the NSCLC PDX model,we co.pared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical *** addition,we detected whole gene expression profiling between primary tumors and PDX *** also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient ***,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care ***:A large co.lection of serially transplantable PDX models for NSCLC were successfully *** histology and pathological immunohistochemistry of PDX xenografts were co.sistent with the patients’tumor *** and RNA-seq further co.firmed that PDX accurately replicated the molecular heterogeneities of primary *** to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and ***:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the co.responding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker disco.ery,and translational research.

关键词： Patient-derived xenograft(PDX) non-small cell lung cancer(NSCLC) tumor heterogeneity

Genetic architecture of maize yield traits dissected by QTL mapping and GWAS in maize

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The Crop Journal 2022年第2期10卷 436-446页

作者： Xiao Zhang Zhiyong Ren Bowen Luo Haixu Zhong Peng Ma Hongkai Zhang Hongmei Hu Yikai Wang Haiying Zhang Dan Liu Ling Wu Zhi Nie Yonghui Zhu Wenzhu He Suzhi Zhang Shunzong Su Yaou Shen Shibin Gao Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureChengdu 611130SichuanChina Guangxi Qingqing Agricultural Technology co. LTD.Nanning 530000GuangxiChina biotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Chengdu 611130SichuanChina g co.lege of Agronomy Sichuan Agricultural UniversityChengdu 611130SichuanChina

The study of yield traits can reveal the genetic architecture of grain yield for improving maize *** this study, an association panel co.prising 362 inbred lines and a reco.binant inbred line population derived from X178 × 9782 were used to identify candidate genes for nine yield traits. High-priority overlap(HPO) genes, which are genes prioritized in a genome-wide association study(GWAS), were investigated using co.xpression networks. The GWAS identified 51 environmentally stable SNPs in two environments and 36 pleiotropic SNPs, including three SNPs with both attributes. Seven hotspots co.taining 41 trait-associated SNPs were identified on six chromosomes by permutation. Pyramiding of superior alleles showed a highly positive effect on all traits, and the phenotypic values of ear diameter and ear weight co.sistently co.responded with the number of superior alleles in tropical and temperate germplasm. A total of 61 HPO genes were detected after trait-associated SNPs were co.bined with the co.xpression networks. Linkage mapping identified 16 environmentally stable and 16 pleiotropic *** SNPs that were located in QTL intervals were assigned as co.sensus SNPs for the yield *** the candidate genes predicted by our study, some genes were co.firmed to function in seed development. The gene Zm00001 d016656 enco.ing a serine/threonine protein kinase was associated with five different traits across multiple environments. Some genes were uniquely expressed in specific tissues and at certain stages of seed development. These findings will provide genetic information and resources for molecular breeding of maize grain yield.

关键词： Maize Yield traits Genome-wide association study(GWAS) Quantitative trait locus(QTL) co.xpression networks

Regulation of different light co.ditions for efficient biomass production and protein accumulation of Spirulina platensis

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Journal of Oceanology and Limnology 2024年第1期42卷 174-186页

作者： Yufei ZHANG Xianjun LI Yuhui LI Shiqi LIU Yanrui CHEN Miao JIA Xin WANG Lu ZHANG Qiping GAO Liang ZHANG Daoyong YU Baosheng GE State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and biotechnology China University of Petroleum(East China)Qingdao 266580China Tongwei co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610093China

Light plays an important role in the photosynthesis and metabolic process of ***,how different light co.ditions regulate the biomass production and protein accumulation of microalgae is mostly *** this study,the influence of different light co.ditions,including light co.ors,densities,and light:dark cycles on the cell growth and biochemical co.position of Spirulina platensis was symmetrically *** different co.ored lights,*** all shows an increase trend within the increased light intensity ranges;however,each showing different optimal light *** the same light intensity,different co.ored lights show different growth rate of *** following the sequence of red>white>green>yellow>*** maximum growth rate and protein accumulation were determined as 21.88 and 5.10 mg/(L·d)when illuminated under red *** energy efficiency of different light sources was calculated and ranked as red>white>blue≈green>*** analysis suggests that red light can promote cell growth and protein accumulation by upregulating genes related to photosynthesis,carbon fixation,and C-N metabolism *** study provides a co.ducive and efficient way to promote biomass production and protein accumulation of *** by regulating light co.ditions.

关键词： microalgae light emitting diode protein accumulation biomass production transcriptomic analysis

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of co.tamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the co.trol group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment co.sisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur co.ld alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur co.ld also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur co.ld downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study co.firms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic Ochratoxin A Protein deposition

Synthesis, characterization and in vivo evaluation of honokiol bisphosphate prodrugs protects against rats’ brain ischemia-reperfusion injury

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Asian Journal of Pharmaceutical Sciences 2019年第6期14卷 640-648页

作者： Gaojie Xu Renghan Dong Jin Liu Li Zhao Yan Zeng Xiaofan Xiao Jinglin An Sheng Huang Yueling Zhong Bing Guang Tai Yang Chengdu Medical co.lege Chengdu 610500China Chengdu Yishan biotechnology co. Ltd.Chengdu 610094China

Honokiol(HK)usage is greatly restricted by its poor aqueous solubility and limited oral *** synthesized and characterized a novel phosphate prodrug of honokiol(HKP)for in vitro and in vivo *** greatly enhanced the aqueous solubility of HK(127.54±15.53 mg/ml)and the stability in buffer solution was sufficient for intravenous *** enzymatic hydrolysis of HKP to HK was extremely rapid in vitro(T 1/2=8.9±2.11 s).Pharmaco.inetics studies demonstrated that after intravenous administration of HKP(32 mg/kg),HKP was co.verted rapidly to HK with a time to reach the maximum plasma co.centration of^5 *** prodrug HKP achieved an improved T 1/2(7.97±1.30 h)and terminal volume of distribution(26.02±6.04 ml/kg)co.pared with direct injection of the equimolar parent drug(0.66±0.01 h)and(2.90±0.342 ml/kg),***,oral administration of HKP showed rapid and improved absorption co.pared with the parent *** was co.firmed to maintain the bioactivity of the parent drug for ameliorating ischemia-reperfusion injury by decreasing brain infarction and improving neurologic *** together,HKP is a potentially useful aqueous-soluble prodrug with improved pharmaco.inetic properties which may merit further development as a potential drug candidate.

关键词： Phosphate prodrug Honokiol Pharmaco.inetics Focal cerebral ischemia-reperfusion

Zearalenone toxico.is on reproduction as estrogen receptor selective modulator and alleviation of zearalenone biodegradative agent in pregnant sows

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Journal of Animal Science and biotechnology 2022年第4期13卷 1222-1232页

作者： Jianchuan Zhou Lihong Zhao Shimeng Huang Qingxiu Liu Xiang Ao Yuanpei Lei Cheng Ji Qiugang Ma State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China sichuan tieqilishi Food co. LtdMianyang 610000Sichuan provinceChina.

Background:Zearalenone(ZEA)is a resorcylic acid lactone derivative derived from various Fusarium species that are widely found in food and *** molecular structure of ZEA resembles that of the mammalian hormone 17β-oestradiol,thus zearalenone and its metabolites are known to co.pete with endogenous hormones for estrogen receptors binding sites and to activate transcription of oestrogen-responsive ***,the effect of long-term low-dose ZEA exposure on the reproductive response to Bacillus subtilis ANSB01G culture for first-parity gilts has not yet been *** study was co.ducted to investigate the toxic effects of ZEA as an estrogen receptor selective modulator and the alleviating effects of Bacillus subtilis ANSB01G cultures as ZEA biodegraders in pregnant sows during their first ***:A total of 80 first-parity gilts(Yorkshire×Landrace)were randomly assigned to four dietary treatments during gestation:co.positive co.trol);MO(negative co.trol,246μg ZEA/kg diet);co.(co.*** ANSB01G culture with 2×10^(9)CFU/kg diet);MOA(MO+*** ANSB01G culture with 2×10^(9)CFU/kg diet).There were 20 replications per treatment with one gilt per *** low-dose ZEA naturally co.taminated diets disordered most of reproductive hormones secretion and affected estrogen receptor-αand estrogen receptor-βco.centrations in serum and specific organs and led to moderate histopathological changes of gilts,but did not cause significant detrimental effects on reproductive *** addition of Bacillus subtilis ANSB01G culture to the diet can effectively relieve the co.petence of ZEA to estrogen receptor and the disturbance of reproductive hormones secretion,and then ameliorate toxico.is of ZEA in ***:co.lectively,our study investigated the effects of feeding low-dose ZEA on reproduction in pregnant sows during their first *** low-dose ZEA co.ld modulate estrogen receptor-αand-βco.centrations in specific organs,ca

关键词： Bacillus subtilis ANSB01G Estrogen receptor Myco.oxin biodegradation Pregnant sows Reproductive performance ZEA

Implications of vitamin D for flesh quality of grass carp(Ctenopharyngodon idella):antioxidant ability,nutritional value,sensory quality,and myofiber characteristics

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Journal of Animal Science and biotechnology 2023年第6期14卷 2596-2613页

作者： Yao Zhang Chaonan Li Xiaoqiu Zhou Weidan Jiang Pei Wu Yang Liu Hongmei Ren Lu Zhang Haifeng Mi Jiayong Tang Ruinan Zhang Lin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina. Fish Nutrition and Safety Production University Key Laboratory of sichuan Province Sichuan Agricultural UniversityChengdu 611130China. Key Laboratory for Animal Disease‑Resistant Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 611130China. Healthy Aquaculture Key Laboratory of sichuan Province Tongwei Co.Ltd.Chengdu 610041SichuanChina.

Background Muscle represents a unique and co.plex system with many co.ponents and co.prises the major edible part of *** D is a critical nutrient for animals and is known to enhance calcium absorption and immune *** recent years,dietary vitamin D supplementation in livestock has received increased attention due to biological responses including improving shear force in mammalian ***,the vitamin D acquisition and myofiber development processes in fish differ from those in mammals,and the effect of vitamin D on fish flesh quality is poorly ***,the influence of dietary vitamin D on fillet quality,antioxidant ability,and myofiber development was examined in grass carp(Ctenopharyngodon idella).Methods A total of 540 healthy grass carp,with an initial average body weight of 257.24±0.63 g,were allotted in 6 experimental groups with 3 replicates each,and respectively fed co.responding diets with 15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg vitamin D for 70 *** Supplementation with 1,167.9 IU/kg vitamin D significantly improved nutritional value and sensory quality of fillets,enhancing crude protein,free amino acid,lipid,and co.lagen co.tents;maintaining an ideal pH;and reduc-ing lactate co.tent,shear force,and co.king loss relative to respective values in the co.trol(15.2 IU/kg)*** myofiber diameter and the frequency of myofibers>50μm in diameter increased under supplementation with 782.5–1,167.9 IU/kg vitamin *** of oxidative damage biomarkers decreased,and the expression of antioxi-dant enzymes and nuclear factor erythroid 2-related factor 2 signaling molecules was upregulated in the 1,167.9 IU/kg vitamin D treatment co.pared to respective values in the co.trol ***,vitamin D supplementation activated cell differentiation by enhancing the expression of myogenic regulatory factors and myocyte enhancer factors co.pared to that in the co.trol *** addition,supplementation with 1,167.9 IU/kg

关键词： Antioxidant Grass carp Myofiber development Nutritional value Sensory quality Vitamin D

Transcription factor ZmNAC126 plays an important role in transcriptional regulation of maize starch synthesis-related genes

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The Crop Journal 2021年第1期9卷 192-203页

作者： Qianlin Xiao Yayun Wang Hui Li Chunxia Zhang Bin Wei Yongbin Wang Huanhuan Huang Yangping Li Guowu Yu Hanmei Liu Junjie Zhang Yinghong Liu Yufeng Hu Yubi Huang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina co.lege of Agronomy and biotechnology Southwest UniversityChongqing 400716China co.lege of Agronomy Sichuan Agricultural UniversityChengdu 611130SichuanChina co.lege of Life Science Sichuan Agricultural UniversityYa'an 625014SichuanChina Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina sichuan Seed Station Chengdu 610041SichuanChina

Maize(Zea mays L.)is one of the most important food crops in the world,and starch is the main co.ponent of its *** regulation plays a vital role in starch ***,it is not well understood in *** report the identification of the transcription factor ZmNAC126 and its role in regulation of starch synthesis in *** expression of ZmNAC126 was higher in maize endosperm and kernels than in roots or ***126 shared a similar expression pattern with starch synthesis genes during seed development,and its expression pattern was also co.sistent with the accumulation of ***126 is a typical transcription factor with a transactivation domain between positions 201 and 227 of the amino acid sequence,is located in the nucleus,and binds to CACG repeats in *** one-hybrid assay revealed that ZmNAC126 bound the promoters of ZmGBSSI,ZmSSIIa,ZmSSIV,ZmISA1,and *** overexpression of ZmNAC126 in maize endosperm increased the activities of promoters pZmSh2,pZmBt2,pZmGBSSI,pZmSSIIIa,and pZmBT1 but inhibited the activities of pZmISA1 and ***126 thus acts in starch synthesis by transcriptionally regulating targeted starch synthesis-related genes in maize kernels.

关键词： Maize Starch synthesis ZmNAC126 co.expression Transcription regulation