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Diatom-inspired multiscale mineralization of patterned protein–polysaccharide complex structures

National Science Review 2021年第8期8卷 186-197页

作者： Ke Li Yingfeng Li Xinyu Wang Mengkui Cui Bolin An Jiahua Pu Jintao Liu Boyang Zhang Guijun Ma Chao Zhong School of Physical Science and Technology Shanghai Tech University Center for Materials Synthetic Biology Shenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of Sciences CAS key laboratory of Quantitative engineering Biology Shenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

marine diatoms construct their hierarchically ordered, three-dimensional(3D) external structures called frustules through precise biomineralization processes. Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites. Here, we report the construction of highly ordered,mineralized composites based on fabrication of complex self-supporting porous structures—made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin—and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2and Ga2O3. Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals,thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution. Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.

关键词： biomimetic mineralization patterned porous structure genetic engineering amyloid protein artificial photosynthesis

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Motion and Dynamic Responses of A Semisubmersible in Freak Waves

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China Ocean engineering 2017年第6期31卷 754-763页

作者： LI Xin DENG Yan-fei LI Lei TIAN Xin-liang LI Jun State key laboratory of Ocean engineering Shanghai Jiao Tong University Shanghai 200240 China CIMC Offshore Co. Ltd. Shenzhen 518000 China

The present research aims at clarifying the effects of freak wave on the motion and dynamic responses of a semisubmersible. To reveal the effects of mooring stiffness, two mooring systems were employed in the model tests and time-domain simulations. The 6-DOF motion responses and mooring tensions have been measured and the 3- DOF motions of fairleads were calculated as well. From the time series, trajectories and statistics information, the interactions between the freak wave and the semisubmersible have been demonstrated and the effects of mooring stiffness have been identified. The shortage of numerical simulations based on 3D potential flow theory is presented. Results show that the freak wave is likely to cause large horizontal motions for soft mooring system and to result in extremely large mooring tensions for tight mooring system. Therefore, the freak wave is a real threat for the marine structure, which needs to be carefully considered at design stage.

关键词： motion response mooring tension semisubmersible freak wave mooring stiffness

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Single-cell brain atlas of Parkinson's disease mouse model

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Journal of Genetics and Genomics 2021年第4期48卷 277-288页

作者： Jixing Zhong Gen Tang Jiacheng Zhu Weiying Wu Ge Li Xiumei Lin Langchao Liang Chaochao Chai Yuying Zeng Feiyue Wang Lihua Luo Jiankang Li Fang Chen Zhen Huang Xiuqing Zhang Yu Zhang Hongde Liu Xin Qiu Shengping Tang Dongsheng Chen School of Biological Science&Medical engineering Southeast UniversityNanjing 210096China BGI-shenzhen Shenzhen 518083China shenzhen Children's Hospital Shenzhen 518083China BGI Education Center University of Chinese Academy of SciencesShenzhen 518083China Department of Neurobiology NHC and CAMS Key Laboratory of Medical NeurobiologySchool of Brain Science and Brian MedicineAnd the MOE Frontier Science Center for Brain Research and Brain-Machine IntegrationZhejiang University School of MedicineHangzhou 310012China Guangdong Provincial key laboratory of laboratory Animals Guangdong Laboratory Animals Monitoring InstituteGuangzhouGuangdong 510663China MGI BGI-ShenzhenShenzhen 518083China Life Science College Fujian Normal UniversityFuzhou 350108China key laboratory of Special marine Bio-resources Sustainable Utilization of Fujian Province Fuzhou 350108China

Parkinson's disease(PD)is a neurodegenerative disease,leading to the impairment of movement *** pathogenesis has been largely investigated,either limited to bulk transcriptomic levels or at certain cell types,which failed to capture the cellular heterogeneity and intrinsic interplays among distinct cell ***,we report the application of single-nucleus RNA-seq on midbrain,striatum,and cerebellum of theα-syn-A53 T mouse,a well-established PD mouse model,and matched controls,generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual ***,we comprehensively depicte the dysfunctions in PD pathology,covering the elevation of NF-k B activity,the alteration of ion channel components,the perturbation of protein homeostasis network,and the dysregulation of glutamatergic ***,we identify a variety of cell types closely associated with PD risk *** together,our study provides valuable resources to systematically dissect the molecular mechanism of PD pathogenesis at the single-cell resolution,which facilitates the development of novel approaches for diagnosis and therapies against PD.

关键词： Parkinson’s disease α-syn-A53T Single-nucleus RNA-Seq Pathogenesis Neuroinflammtion

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The pseudocapacitance mechanism of graphene/CoAl LDH and its derivatives:Are all the modifications beneficial?

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Journal of Energy Chemistry 2021年第1期30卷 218-227,I0007页

作者： Chuan Jing Xu Dong Liu Kailin Li Xiaoying Liu Biqin Dong Fan Dong Yuxin Zhang State key laboratory of Mechanical Transmissions College of Materials Science and EngineeringChongqing UniversityChongqing 400044China Research Centre of Laser Fusion China Academy of Engineering PhysicsMianyang 621900SichuanChina engineering Research Center for Waste Oil Recovery Technology and Equipment of Ministry of Education Chongqing Key Laboratory of Catalysis and New Environmental MaterialsCollege of Environment and ResourcesChongqing Technology and Business UniversityChongqing 400067China Guangdong Provincial key laboratory of Durability for marine Civil engineering Shenzhen UniversityShenzhen 518060GuangdongChina Research Center for Environmental Science&Technology Institute of Fundamental and Frontier SciencesUniversity of Electronic Science and Technology of ChinaChengdu 611731SichuanChina

Cobalt-Aluminum layered double hydroxide(CoAl LDH) is a hopeful electrode material due to the advantage of easy modifiability for preparing LDH-based ***,there is short of modification methods to prepare the Co-based derivatives from CoAl LDH and also short of an intuitive perspective to analyze the pseudocapacitance mechanism of CoAl LDH and its ***,Graphene/CoAl LDH and its derivatives including Graphene/CoS,Graphene/CoS-1,Graphene/CoOOH,Graphene/CoP were prepared by reasonably using alkali etching treatment,sulfofication and *** specific capacitance of Graphene/CoAl LDH,Graphene/CoS,Graphene/CoS-1,Graphene/CoOOH,Graphene/CoP at1 A g^(-1) are 260.7,371.3,440.8,61.4 and 122.2 F g^(-1),*** pseudocapacitance mechanism of Graphene/CoAl LDH and its derivatives was *** to the positive effect of sulfofication on the electrical conductivity of GO and cobalt sulfide,the Graphene/CoS and Graphene/CoS-1 exhibit the optimal electrochemical performance and superior rate *** addition,due to the repulsion effect between Graphene and OH-,the Graphene/CoAl LDH exhibits optimal cycling stability of 224.1% capacitance retention after 20000 ***,the reason of terrible specific capacitance of Graphene/CoOOH is that the presence of H bond in interlayer of CoOOH inhibits the interaction between Co3+ and ***,not all modifications will increase the specific capacitance of the electrode ***,this work provides us with a detailed analysis of the electrochemical mechanism and correlation of CoAl LDH and its derivatives from the perspective of crystal structure and composition.

关键词： CoAl LDH and its derivates Modification Pseudocapacitance mechanism Alkali etching treatment Sulfofication Phosphorization

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Evaluation of the submarine debris-flow hazard risks to planned subsea pipeline systems: a case study in the Qiongdongnan Basin, South China Sea

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Acta Oceanologica Sinica 2023年第5期42卷 139-153页

作者： Mingquan Huang Xuesheng Qian Jingping Xu Xuecheng Li School of Environment Harbin Institute of TechnologyHarbin 150001China Department of Ocean Science and engineering Southern University of Science and TechnologyShenzhen 518055China Southern marine Science and engineering Guangdong laboratory(Guangzhou) Guangzhou 511458China China Offshore Fugro Geosolutions(shenzhen)Corporation Limited Shenzhen 518067China

The ever-increasing deepwater oil and gas development in the Qiongdongnan Basin,South China Sea has initiated the need to evaluate submarine debris-flow hazard risks to seafloor *** paper presents a case study on evaluating the debris-flow hazard risks to the planned pipeline systems in this *** used a numerical model to perform simulations to support this quantitative ***,one relict failure interpreted across the development site was *** back-analysis modeling was used to validate the applicability of the rheological ***,this model was applied to forecast the runout behaviors of future debris flows originating from the unstable upslope regions considered to be the most critical to the pipeline systems surrounding the Manifolds A and *** model results showed that the potential debris-flow hazard risks rely on the location of structures and the selection of rheological *** the Manifold B and connected pipeline systems,because of their remote distances away from unstable canyon flanks,the potential debris flows impose few ***,the pipeline systems around the Manifold A are exposed to significant hazard risks from future debris flows with selected rheological *** results are beneficial for the design of a more resilient pipeline route in consideration of future debris-flow hazard risks.

关键词： submarine debris flow pipeline manifold hazard evaluation route optimization Qiongdongnan Basin

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Mixed Carbonate-Siliciclastic Deposits in a Channel Complex in the Northern South China Sea

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Journal of Earth Science 2018年第3期29卷 707-720页

作者： Benjun Ma Shiguo Wu Lijun Mi Thomas Lüdmann Jinwei Gao Wei Gao key laboratory of marine Geology and Environment Institute of OceanologyChinese Academy of SciencesQingdao 266071China key laboratory of marine Georesources and Prospecting Institute of Deep-sea Science and Engineering Chinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China Functional laboratory for marine Geology and Environment Qingdao National Laboratory for Marine Science and TechnologyQingdao 266061China shenzhen Branch China National Offshore Oil CorporationGuangzhou 510420China Institute of Geology Centre for Earth System Research and SustainabilityUniversity of HamburgHamburg 20146Germany

New high-resolution 3D seismic data image a submarine channel complex in the northern slope of the South China Sea. The channel complex stretches hundreds of kilometers across the slope and flows into the deepsea from the siliciclastic shelf margin, linking neritic environment to the pelagic plain. The evolution of the channel complex developed two sedimentary stages, stage Ⅰ （19.1-18.5 Ma） and stage Ⅱ （18.5-17.5 Ma）, separated by erosional surfaces. In the first stage, the complex was rifled with pure siliciclastic sediments, forming thick-massive sandstone intercalated by thin layers of mudstone. During the stage Ⅱ, the channel complex was deposited five carbonate-siliciclastic cycles. The unexpected channel-fifl carbonate deposits present allochthonous characteristics, suggesting the siliciclastic channel was surprisingly used to transport carbonate sediment from the adjacent neritic carbonate platform. By analyzing the periodical carbonate sedimentary process in the siliciclastic channel complex, we infer that it was related to the in situ carbonate production of the neritic carbonate platform and was most likely to be controlled by the relative sea-level changes. Unlike line-source carbonate slope aprons or smafl-sized carbonate channels, the large-sized siliciclastic channel complex links directly neritic carbonate platform to deepwater basin and can transport large volumes of neritic carbonates to the pelagic environment in a short period. The new findings help to estimate the contributions of neritie siliciclastic shelf and carbonate platform to deepwater slope more accurately. This study suggests that channel systems are more complex than expected and have significant implications on the conceptual models describing the deepwater sedimentary theory.

关键词： submarine channels mixed carbonate-siliciclastic deposits allochthonous carbonate sediment flux sedimentology South China Sea.

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Convergence of Broadband and Broadcast/Multicast in Maritime Information Networks

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Tsinghua Science and Technology 2021年第5期26卷 592-607页

作者： Jun Du Jian Song Yong Ren Jintao Wang the Department of Electronic engineering Tsinghua UniversityBeijing 100084China the Institute for Ocean engineering Tsinghua UniversityBeijing 100084 Peng Cheng laboratory Shenzhen 518055China the Beijing National Research Center for Information Science and Technology Tsinghua UniversityBeijing 100084 the key laboratory of Digital TV System of Guangdong Province and shenzhen City Research Institute of Tsinghua UniversityShenzhen 518057China

Recently,the fifth-generation(5G)of wireless networks mainly focuses on the terrestrial ***,the well-developed emerging technologies in 5G are hardly applied to the maritime communications,resulting from the lack of communication infrastructure deployed on the vast ocean,as well as different characteristics of wireless propagation environment over the sea and maritime user *** satisfy the expected plethora of broadband communications and multimedia applications on the ocean,a brand-new maritime information network with a comprehensive coverage capacity in terms of all-hour,all-weather,and all-sea-area has been expected as a revolutionary paradigm to extend the terrestrial capacity of enhanced broadband,massive access,ultra-reliable,and low-latency to the vast *** considering the limited available resource of maritime communication infrastructure,the convergence of broadband and broadcast/multicast can be regarded as a possible yet practical solution for realizing an efficient and flexible resource configuration with high quality of ***,according to such multi-functionality and all-coverage maritime information network,the monitoring and sensing of vast ocean area relying on massive Ocean of Things and advanced radar techniques can be also *** these issues above,this study proposes a Software Defined Networking(SDN)based Maritime Giant Cellular Network(MagicNet)architecture for broadband and multimedia *** on this network,the convergence techniques of broadband and broadcast/multicast,and their supporting for maritime monitoring and marine sensing are also introduced and surveyed.

关键词： Maritime Giant Cel ular Network(Magic Net) integrated broadcast-multicast-unicast communications maritime multimedia services Ocean of Things(OoT) al-coverage

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Frictional sliding of infilled planar granite fracture under oscillating normal stress

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International Journal of Mining Science and Technology 2023年第6期33卷 687-701页

作者： Kang Tao Wengang Dang Yingchun Li School of Civil engineering Sun Yat-sen UniversityZhuhai 519086China State key laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China Southern marine Science and engineering Guangdong laboratory Zhuhai 519086China State key laboratory of Coastal and Offshore engineering Dalian University of TechnologyDalian 116024China

This paper investigates the frictional behavior of the infilled rock fracture under dynamic normal stress.A series of direct shear tests were conducted on saw-cut granite fractures infilled with quartz using a selfdeveloped dynamic shear apparatus,and the effects of normal load oscillation amplitude,normal load oscillation period and sliding velocity were *** test results reveal that the shear response can be divided into three stages over a whole loading-unloading process,characterized by different time spans and stress ***,a smaller oscillation amplitude,a longer oscillation period and a fast shear velocity promote the stability of the friction system,which is also confirmed by the Coulomb failure criterion calculated based on the observed periodic apparent friction *** dynamic strengthening/weakening phenomenon is dependent on the oscillation amplitude and product of sliding velocity and oscillation period(vT).Also,the rate and state friction law incorporating the parameter a that characterizes the normal stress variation is employed to describe the dynamic friction coefficient but exhibits an incompetent performance when handling intensive variation in normal ***,the potential seismicity induced by oscillating normal stress based on the observed stress drop is *** work helps us understand the sliding process and stability evolution of natural faults,and its benefits for relative hazard mitigation.

关键词： Granite fracture Quartz gouge Normal load oscillation Frictional stability Rate and state friction law

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Mesozoic subduction-accretion zone in northeastern South China Sea inferred from geophysical interpretations

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Science China Earth Sciences 2006年第5期49卷 471-482页

作者： ZHOU Di1, WANG Wanyin2, WANG Jialin3, PANG Xiong4, CAI Dongsheng5 & SUN Zhen1 1. key laboratory of Marginal Sea Geology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guang- zhou 510301, China 2. College of Geological and Topographical engineering, Chang’an University, Xi’an 710054, China 3. key laboratory of marine Geology, Tongji University, Shanghai 200092, China 4. China National Offshore Oil Co. Limited – shenzhen Branch, shenzhen 518067, China 5. China National Offshore Oil Co. Limited – Research Center, Beijing 100027, China key laboratory of Marginal Sea Geology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou China College of Geological and Topographical engineering Chang’an University Xi’an China key laboratory of marine Geology Tongji University Shanghai China China National Offshore Oil Co. Limited — shenzhen Branch Shenzhen China China National Offshore Oil Co. Limited — Research Center Beijing China

A segment of Mesozoic subduction-accretion zone was inferred across thenortheastern South China Sea at approximately NE450 orientation. Basic evidence includes thefollowing: A belt of peek gross horizontal Bouguer gravity gradient (PGHGBA) is comparable in sizeand intensity to that of the Manila subduction-accretion zone. A belt of high positive magneticanomalies appears to the north and sub-parallel to the PGHGBA, representing the volcanic arcassociated to the subduction zone. The PGHGBA crosses obliquely both Cenozoic structures and presentseafloor topography, indicating a pre-Cenozoic age. The segment is offset left-laterally byNW-running strike-slip faults, in concord with the Mesozoic stress field of South China. Inaddition, the existence of the subduction zone is supported by wide-angle seismic data obtained indifferent years by different institutions. At approximate localities, a north-dipping ramp of Mohosurface is indicated by records of ocean-bottom seismometers, and a strong reflector about 8 kmbeneath the Moho reflector is indicated by both OBS and long-cable seismic records. Theidentification of a segment of Mesozoic subduction zone in NE South China Sea fills nicely the gapof the Great Late Mesozoic Circum SE Asia Subduction-acrretion Zone, which extended from Sumatra,Java, SE Kalimantan to N Palawan, and from Taiwan, Ryukyu to SW Japan.

关键词： Mesozoic, subduction zone, SE Asia, NE South China Sea, geophysics.

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Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm

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Chinese Journal of Oceanology and Limnology 2011年第6期29卷 1209-1223页

作者：高峥王鑫 Angelos K.HANNIDES Francis J.SANSONE 汪光义 State key laboratory of Crop Biology College of Life Sciences Shandong Agricultural University Department of Oceanography University of Hawaii shenzhen engineering laboratory for Algal Biofuel Technology Development and Application School of Environment and Energy Peking University shenzhen Graduate School

Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments, its impact on microbial communities remains largely unknown. In this study, we applied denaturing gradient gel electrophoresis （DGGE） and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units （OTU） were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions, including Proteobacteria （Alphas, Beta-, Gamma-, Delta-, and Epsilonproteobacteria）, Aetinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Verrucomicrobia, Spirochaetes, and the candidate divisions WS3, SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments, but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA, gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments, with the highest diversity found in the anoxic layer （15-25 mm） and the least diversity in the suboxic layer （3-5 mm）. Analysis of the nosZ, and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers, with their highest diversity being in the anoxic and oxic sediment layers, respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.

关键词： bacterial diversity bacterial stratification biogeochemical gradients sandy reef sediments

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