Single-cell brain atlas of Parkinson's disease mouse model

作     者：Jixing Zhong Gen Tang Jiacheng Zhu Weiying Wu Ge Li Xiumei Lin Langchao Liang Chaochao Chai Yuying Zeng Feiyue Wang Lihua Luo Jiankang Li Fang Chen Zhen Huang Xiuqing Zhang Yu Zhang Hongde Liu Xin Qiu Shengping Tang Dongsheng Chen Jixing Zhong;Gen Tang;Jiacheng Zhu;Weiying Wu;Ge Li;Xiumei Lin;Langchao Liang;Chaochao Chai;Yuying Zeng;Feiyue Wang;Lihua Luo;Jiankang Li;Fang Chen;Zhen Huang;Xiuqing Zhang;Yu Zhang;Hongde Liu;Xin Qiu;Shengping Tang;Dongsheng Chen

作者机构：School of Biological Science&Medical EngineeringSoutheast UniversityNanjing 210096China BGI-ShenzhenShenzhen 518083China Shenzhen Children's HospitalShenzhen 518083China BGI Education CenterUniversity of Chinese Academy of SciencesShenzhen 518083China Department of NeurobiologyNHC and CAMS Key Laboratory of Medical NeurobiologySchool of Brain Science and Brian MedicineAnd the MOE Frontier Science Center for Brain Research and Brain-Machine IntegrationZhejiang University School of MedicineHangzhou 310012China Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring InstituteGuangzhouGuangdong 510663China MGIBGI-ShenzhenShenzhen 518083China Life Science CollegeFujian Normal UniversityFuzhou 350108China Key Laboratory of Special Marine Bio-resources Sustainable Utilization of Fujian ProvinceFuzhou 350108China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2021年第48卷第4期

页      面：277-288页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(31702074 and 31872309) Sanming Project of Medicine in Shenzhen(SZSM202011012) Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20170412153100794) 

主　　题：Parkinson’s disease α-syn-A53T Single-nucleus RNA-Seq Pathogenesis Neuroinflammtion 

摘      要：Parkinson s disease(PD)is a neurodegenerative disease,leading to the impairment of movement *** pathogenesis has been largely investigated,either limited to bulk transcriptomic levels or at certain cell types,which failed to capture the cellular heterogeneity and intrinsic interplays among distinct cell ***,we report the application of single-nucleus RNA-seq on midbrain,striatum,and cerebellum of theα-syn-A53 T mouse,a well-established PD mouse model,and matched controls,generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual ***,we comprehensively depicte the dysfunctions in PD pathology,covering the elevation of NF-k B activity,the alteration of ion channel components,the perturbation of protein homeostasis network,and the dysregulation of glutamatergic ***,we identify a variety of cell types closely associated with PD risk *** together,our study provides valuable resources to systematically dissect the molecular mechanism of PD pathogenesis at the single-cell resolution,which facilitates the development of novel approaches for diagnosis and therapies against PD.