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CRIsPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of sARs-CoV-2 Delta Variant

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Nano-Micro Letters 2022年第10期14卷 83-94页

作者： Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy sWageh Abdullah GAl-sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie han Zhang International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China shenzhen International Institute for Biomedical Research Shenzhen 518116People’s Republic of China Department of Respiratory Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Institute of Pediatrics Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China shenzhen han’s tech limited company Shenzhen 518000People’s Republic of China Hospital of Guangzhou Medical University Qingyuan city People’s HospitalQingyuan 511518People’s Republic of China Department of Physics Faculty of ScienceKing Abdulaziz UniversityJeddah 21589Saudi Arabia Research Center for Advanced Materials science(RCAMs) King Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Zhongmin(shenzhen)Intelligent Ecology Co. LtdShenzhen 518055People’s Republic of China shenzhen Center for Disease Control and Prevention Shenzhen 518055People’s Republic of China

Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(sARs-CoV-2).The gold standard method for the diagnosis of sARs-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing ***,we first proposed a robust technique-methodology of electrochemical CRIsPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of sARs-CoV-2 *** enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold *** DNA template identical to sARs-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification *** detection can be completed within 1 h with high stability and specificity which benefits from the CRIsPR-Cas ***,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.

关键词： sARs-COV-2 variant Methodology of electrochemical CRIsPR sensing(MOECs) Gold nanoparticles(AuNPs) Point-of-care testing(POCT)

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A CRIsPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of sARs-CoV-2

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National science Review 2022年第8期9卷 100-109页

作者： Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar A.Al-Hartomy swelm Wageh Abdullah G.Al-sehemi Zhiguang Luo Jiangtian Yu Yonghong shao Defa Li shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie han Zhang shenzhen Engineering Laboratory of Phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics science and technology of Ministry of Education 3. shenzhen Institute of Translational Medicine 4. Department of Otolaryngology Shenzhen Second People’s Hospital 5. the First Affiliated Hospital 6. Institute of Microscale Optoelectronics Shenzhen University 7. Shenzhen International Institute for Biomedical Research 8. Shenzhen Han’s Tech Limited Company 9. Shenzhen Metasensing Tech Limited Company Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials science (RCAMs) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning sARs-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive’.Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time,we have developed MOPCs(Methodologies of Photonic CRIsPR sensing), which combines an optical sensing technology-surface plasmon resonance(sPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat(CRIsPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCs is a low-cost, CRIsPR/Cas12a-systemempowered sPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCs achieves a highly sensitive analysis of sARs-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRIsPR-empowered sPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

关键词： surface plasmon resonance CRIsPR sARs-CoV-2 Omicron variant

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Correlations between shortening Rate,Uplift Rate,and Inversion Rate in Central Inversion Zone of Xihu Depression,East China sea Basin

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Journal of China University of Geosciences 2009年第4期20卷 699-708页

作者：杨风丽于海啸张青林李前裕 state Key Laboratory of Marine Geology Tongji University shenzhen Oil company CNOOC

Late Miocene shortening rate, uplift rate, and inversion rate in the central inversion zone of the Xihu （西湖） depression, East China sea basin, were independently determined from a large number of 2D reflection seismic data and methods. shortening rate was estimated from the 2DMOVE balanced cross-section technology, uplift rate was calculated after absolute erosions were determined from seismic data during the uplifting time, and inversion rate was measured using the improved calculation method by song （1997）. The cross correlations among shortening rate, uplift rate, and inversion rate show a good positive relationship, with some differences existing in local areas. This article analyzes the cross correlation between these structural rates and discusses the dynamics of mechanisms for basin inversion and their influence on hydrocarbon accumulation.

关键词： basin inversion shortening rate uplift rate inversion rate cross correlation Xihu depression East China sea basin.

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spectrum-sensing Method for Arc Fault Detection in Direct Current system with Lithium Batteries

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Journal of shanghai Jiaotong university(science) 2023年第5期28卷 630-637页

作者：韩正谦罗利文姚伟尹邵文陈伟王营辉 Key Laboratory of Control of Power Transmission and Conversion of Ministry of Education Shanghai Jiao Tong UniversityShanghai 200240China FinDreams Powertrain BYD Auto Industry Company LimitedShenzhen 518118GuangdongChina

We mainly study the detection of arc faults in the direct current (DC) system of lithium battery energystorage power station. Lithium battery DC systems are widely used, but traditional DC protection devices areunable to achieve adequate protection of equipment and circuits. We build an experimental platform based onan energy storage power station with lithium batteries. Then, the data collection of normal current and arc-faultcurrent is completed under multiple conditions, and the waveforms of obvious and weak signals as the arc occursare presented. We analyze the principles and application range of several common spectrum-sensing methods andstudy the feasibility of applying them to the arc detection field. Finally, the covariance absolute value detectionalgorithm is selected, and the average value of the current is filtered out to make the algorithm adapt to the arcdetection field. The result shows that the detection probability in 500 sets of experimental data has reached 98%.

关键词： arc fault energy storage power station spectrum sensing covariance matrix

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Recent Advancements on Photothermal Conversion and Antibacterial Applications over MXenes-Based Materials

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Nano-Micro Letters 2022年第11期14卷 107-142页

作者： shuyan Hao Hecheng han Zhengyi Yang Mengting Chen Yanyan Jiang Guixia Lu Lun Dong Hongling Wen Hui Li Jiurong Liu Lili Wu Zhou Wang Fenglong Wang Key Laboratory for Liquid-solid structural Evolution and Processing of Materials Ministry of Education Shandong UniversityJinan 250061People’s Republic of China Department of Virology School of Public HealthShandong UniversityJinan 250012People’s Republic of China shenzhen Research Institute of shandong University A301 Virtual University Park in South District of Nanshan High-Tech ZoneShenzhen 518057People’s Republic of China school of Civil Engineering Qingdao University of TechnologyQingdao 266033People’s Republic of China Department of Breast surgery Qilu HospitalShandong UniversityJinan 250012People’s Republic of China

The pernicious bacterial proliferation and emergence of super-resistant bacteria have already posed a great threat to public health,which drives researchers to develop antibiotic-free strategies to eradicate these fierce *** enormous achievements have already been achieved,it remains an arduous challenge to realize efficient sterilization to cut off the drug resistance ***,photothermal therapy(PTT)has emerged as a promising solution to efficiently damage the integrity of pathogenic bacteria based on hyperthermia beyond their *** now,numerous photothermal agents have been studied for antimicrobial *** them,MXenes(a type of two-dimensional transition metal carbides or nitrides)are extensively investigated as one of the most promising candidates due to their high aspect ratio,atomic-thin thickness,excellent photothermal performance,low cytotoxicity,and ultrahigh dispersibility in aqueous ***,the enormous application scenarios using their antibacterial properties can be tailored via elaborated designs of MXenes-based *** this review,the synthetic approaches and textural properties of MXenes have been systematically presented first,and then the photothermal properties and sterilization mechanisms using MXenes-based materials are ***,recent progress in diverse fields making use of the photothermal and antibacterial performances of MXenes-based materials are well summarized to reveal the potential applications of these materials for various purposes,including in vitro and in vivo sterilization,solar water evaporation and purification,and flexible antibacterial *** but not least,the current challenges and future perspectives are discussed to provide theoretical guidance for the fabrication of efficient antimicrobial systems using MXenes.

关键词： MXenes Antibacterial mechanisms Photothermal properties Antibacterial applications

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Clinical outcomes in patients with stage non-seminomatous germ cell cancer

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Asian Journal of Andrology 2013年第4期15卷 558-563,I0011页

作者： Zhao-Jie Lv song Wu Pei Dong Kai Yao Yin-Yin He Yao-Ting Gui Fang-Jian Zhou Zhuo-Wei Liu Zhi-Ming Cai shenzhen second People's Hospital the First Affiliated Hospital of Shenzhen University Shenzhen 518036 China LAnhui Medical University Hefei 230032 China Department of Urology Sun Yat-Sen University Cancer Centre Guangzhou 510060 China Institute of Immunology Zhongshan School of Medicine Sun Yat-Sen University Guangzhou 510060 China and Institute of Urology Shenzhen PKU-HKUST Medical Centre Shenzhen 518036 China

This study assesses the long-term outcomes in han Chinese patients with clinical stage I non-seminomatous germ cell testicular cancer （CsI NsGCT） treated with surveillance, retroperitoneal lymph node dissection （RPLND） and adjuvant chemotherapy. We retrospectively evaluated 89 patients with a mean age of 26.5 years. After orchiectomy, 37 patients were treated with surveillance, 34 underwent RPLND and 18 were managed with chemotherapy. The overall survival rate, the recurrence-free survival rate and the risk factors were evaluated. The median follow-up length was 92 months （range： 6-149 months）. Thirteen of the 89 patients （14.6%） had relapses, and one died by the evaluation date. The overall survival rate was 98.9%. The cumulative 4-year recurrence-free rates were 80.2%, 92.0% and 100% for the surveillance, RPLND and chemotherapy groups, respectively. The disease-free period tended to be briefer in patients with a history of cryptorchidism and those with stage Is. Therefore, surveillance, RPLND and adjuvant chemotherapy might be reliable strategies in compliant patients with CsI NsGCT. surveillance should be recommended for patients with the lowest recurrence rate, especially those without lymphovascular invasion. This study might aid the establishment of a standard therapy for CsI NsGCT in China.

关键词： chemotherapy clinical stage I non-seminomatous germ cell testicular cancer (CsI NsGCT) outcome retroperitoneallymph node dissection (RPLND) surveillance treatment protocols

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Effectiveness and safety of human umbilical cord-mesenchymal stem cells for treating type 2 diabetes mellitus

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World Journal of Diabetes 2022年第10期13卷 877-887页

作者： Xiao-Fen Lian Dong-Hui Lu Hong-Li Liu Yan-Jing Liu Xiu-Qun han Yang Yang Yuan Lin Qing-Xiang Zeng Zheng-Jie Huang Feng Xie Cai-Hao Huang Hong-Mei Wu Ai-Mei Long Ling-Ping Deng Fan Zhang Department of Endocrinology Peking University Shenzhen HospitalShenzhen 518000Guangdong ProvinceChina Department of Research&Development Zhejiang MaiDa Gene Tech Co.LtdZhoushan 316000Zhejiang ProvinceChina Department of Endocrinology Huizhou Central People's HospitalHuizhou 516000Guangdong ProvinceChina Department of Endocrinology Longgang District Central Hospital of ShenzhenShenzhen 518000Guangdong ProvinceChina

BACKGROUND Progressive pancreaticβ-cell dysfunction is a fundamental part of the pathology of type 2 diabetes mellitus(T2DM).Cellular therapies offer novel opportunities for the treatment of T2DM to improve the function of isletβ-*** To evaluate the effectiveness and safety of human umbilical cord-mesenchymal stem cell(hUC-MsC)infusion in T2DM *** sixteen patients were enrolled and received 1×10^(6) cells/kg per week for 3 wk as intravenous hUC-MsC *** effectiveness was evaluated by assessing fasting blood glucose,C-peptide,normal glycosylated hemoglobin A1c(HbA1c),insulin resistance index(homeostatic model assessment for insulin resistance),and isletβ-cell function(homeostasis model assessment ofβ-cell function).The dosage of hypoglycemic agents and safety were evaluated by monitoring the occurrence of any adverse events(AEs).REsULTs During the entire intervention period,the fasting plasma glucose level was significantly reduced[baseline:9.3400(8.3575,11.7725),day 14±3:6.5200(5.2200,8.6900);Ps significantly reduced on day 84±3[baseline:7.8000(7.5250,8.6750),day 84±3:7.150(6.600,7.925);Ps’isletβ-cell function was significantly improved on day 28±3 of intervention[baseline:29.90(16.43,37.40),day 28±3:40.97(19.27,56.36);Psage of hypoglycemic agents was reduced in all patients,of whom 6(50%)had a decrement of more than 50%and 1(6.25%)discontinued the hypoglycemic *** patients had transient fever,which occurred within 24 h after the second or third *** patient(2.08%)had asymptomatic nocturnal hypoglycemia after infusion on day 28±*** liver damage or other side effects were *** The results of this study suggest that hUC-MsC infusion can improve glycemia,restore isletβ-cell function,and reduce the dosage of hypoglycemic agents without serious ***,hUC-MsC infusion may be a novel option for the treatment of T2DM.

关键词： Type 2 diabetes Human umbilical cord mesenchymal stem cells Blood glucose Homeostasis model assessment ofβ-cell function Hypoglycemic agents

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safety and immunogenicity of a mosaic vaccine booster against Omicron and other sARs-CoV-2 variants:a randomized phase 2 trial

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signal Transduction and Targeted Therapy 2023年第2期8卷 672-682页

作者： Nawal Al Kaabi Yun Kai Yang Yu Liang Ke Xu Xue Feng Zhang Yun Kang Yu Qin Jin Jun Wei Hou Jing Zhang Tian Yang salah Hussein Mohamed saif ElDein Ze Hua Lei Hao Zhang shuai shao Zhao Ming Liu Ning Liu Xiang Zheng Ji Guo su sen sen Yang Xiangfeng Cong Yao Tan Wenwen Lei Xue Jun Gao Zhiwei Jiang Hui Wang Meng Li hanadi Mekki Mekki Walid Zaher sally Mahmoud Xue Zhang Chang Qu Dan Ying Liu Jing Zhang Mengjie Yang Islam Eltantawy Peng Xiao Fu Jie shen Jin Juan Wu Zi Bo han Li Fang Du Fang Tang shi Chen Zhi Jing Ma Fan Zheng Ya Nan Hou Xin Yu Li Xin Li Zhao Nian Wang Jin Liang Yin Xiao Yan Mao Jin Zhang Liang Qu Yun Tao Zhang Xiao Ming Yang Guizhen Wu Qi Ming Li sheikh Khalifa Medical City SEHAAbu DhabiUAE College of Medicine and Health sciences Khalifa UniversityAbu DhabiUAE China National Biotec Group company limited BeijingChina The sixth Laboratory National Vaccine and Serum Institute(NVSI)BeijingChina National Engineering Center for New Vaccine Research BeijingChina National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention(China CDC)BeijingChina Clinical Medicine Office National Vaccine and Serum Institute(NVSI)BeijingChina Lanzhou Institute of Biological Products company limited LanzhouChina Beijing Key tech statistical Consulting Co. LtdBeijingChina Beijing Institute of Biological Products company limited BeijingChina Union 71 Abu DhabiUAE G42 Healthcare Abu DhabiUAE

An ongoing randomized,double-blind,controlled phase 2 trial was conducted to evaluate the safety and immunogenicity of a mosaic-type recombinant vaccine candidate,named NVsI-06-09,as a booster dose in subjects aged 18 years and older from the United Arab Emirates(UAE),who had administered two or three doses of inactivated vaccine BBIBP-CorV at least 6 months prior to *** participants were randomly assigned with 1:1 to receive a booster dose of NVsI-06-09 or *** primary outcomes were immunogenicity and safety against severe acute respiratory syndrome coronavirus 2(sARs-CoV-2)Omicron variant,and the exploratory outcome was cross-immunogenicity against other circulating *** May 25 and 30,2022,516 adults received booster vaccination with 260 in NVsI-06-09 group and 256 in BBIBP-CorV *** results showed a similar safety profile between two booster groups,with low incidence of adverse reactions of grade 1 or *** immunogenicity,by day 14 post-booster,the fold rises in neutralizing antibody geometric mean titers(GMTs)from baseline elicited by NVsI-06-09 were remarkably higher than those by BBIBP-CorV against the prototype strain(19.67 vs 4.47-fold),Omicron BA.1.1(42.35 vs 3.78-fold),BA.2(25.09 vs 2.91-fold),BA.4(22.42 vs 2.69-fold),and BA.5 variants(27.06 vs 4.73-fold).similarly,the neutralizing GMTs boosted by NVsI-06-09 against Beta and Delta variants were also 6.60-fold and 7.17-fold higher than those by *** findings indicated that a booster dose of NVsI-06-09 was well-tolerated and elicited broad-spectrum neutralizing responses against divergent sARs-CoV-2 variants,including Omicron and its sub-lineages.

关键词： phase neutral spectrum

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Automated robot and artificial intelligence-powered wastewater surveillance for proactive mpox outbreak prediction

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Biosafety and Health 2024年第4期6卷 225-234页

作者： Guanyong Ou Yuxuan Tang Jiexiang Liu Yabin Hao Zhi Chen Ting Huang shaxi Li a shiyu Niu Yun Peng Jiaqi Feng Hongwei Tu Yang Yang han Zhang Yingxia Liu shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious Diseasethe Third People’s Hospital of ShenzhenSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China shenzhen Clinical Medical College Guangzhou University of Chinese MedicineShenzhen 518116China shenzhen Metasensing tech limited company Shenzhen 518000China Guangdong Provincial Centre for Diseases Control and Prevention Guangzhou 511430China

In the wake of the largest‐ever recorded outbreak of mpox in terms of magnitude and geographical spread in human history since May 2022,we innovatively developed an automated online sewage virus enrichment and concentration robot for disease *** with an artificial intelligence(AI)model,our research aims to estimate mpox cases based on the concentration of the monkeypox virus(MPXV)in *** research has revealed a compelling link between the levels of MPXV in wastewater and the number of clinically confirmed mpox infections,a finding that is reinforced by the ability of our AI prediction model to forecast cases with remarkable precision,capturing 87%of the data’s ***,it is worth noting that this high precision in predictions may be related to the relatively high frequency of data acquisition and the relatively non‐mobile isolated environment of the hospital *** conclusion,this study represents a significant step forward in our ability to track and respond to mpox *** has the potential to revolutionize public health surveillance by utilizing innovative technologies for disease surveillance and prediction。

关键词： Automated online sewage virus enrichment robot Artificial intelligence(AI)model Early warning system Mpox Monkeypox virus(MPXV)

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Highly stretchable and Permeable Conductors Based on shrinkable Electrospun Fiber Mats

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Advanced Fiber Materials 2021年第5期3卷 302-311页

作者： Qingsong Li Chen Ding Wei Yuan Ruijie Xie Xiaomeng Zhou Yang Zhao Mei Yu Zijian Yang Jing sun Qiong Tian Fei han hanfei Li Xinping Deng Guanglin Li Zhiyuan Liu CAs Key Laboratory of Human‑Machine Intelligence‑synergy systems Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences(CAS)Shenzhen 518055China Printable Electronics Research Centre Suzhou Institute of Nano‑Tech and Nano‑BionicsChinese Academy of SciencesSuzhou 215123China

Wearable on-skin electrodes or conductors should be vapor permeable,strain-insensitive,isotropically stretchable and stable under cyclic *** strategies have been proposed to prepare the required conductors up to now;however,it is a challenge to fabricate them with above properties in a simple *** this paper,a highly permeable and stretchable conductor based on electrospun fluorine rubber fiber mat is *** fibers are pre-stretched in electric field during electrospinning,and they shrink isotropically by~35-40%in area after being detached from the *** obtained fiber mat conductor demonstrates high stretchability up to~170%,and the resistance changes only 0.8 under 60%strain,which is superior to many other strain-insensitive *** conductor possesses high stability,no cracks or structure damage are observed after washing and cyclic ***,the conductor is vapor permeable with a water vapor transmission rate of~850 g m−2 day−1,which is comparable to the normal water evaporation in ambient conditions,indicating that it would not disturb the sweat evaporation when being used on *** conductor is successfully used as stretchable yarns and electromyography(EMG)electrodes,showing high reliability in E-textiles and on-skin applications.

关键词： Electrospinning shrinkable fiber mat stretchable conductor Wearable E-textiles EMG signal recording

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