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Storage time affects the level and diagnostic efficacy of plasma biomarkers for neurodegenerative diseases

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Neural Regeneration Research 2025年第8期20卷 2373-2381页

作者： Lifang Zhao Mingkai Zhang Qimeng Li Xuemin Wang Jie Lu Ying Han Yanning Cai Department of clinical Biobank Xuanwu HospitalCapital Medical UniversityBeijingChina Beijing Geriatric Medical Research Center BeijingChina Department of Neurology Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Radiology and Nuclear Medicine Xuanwu HospitalCapital Medical UniversityBeijingChina Beijing Key laboratory of Magnetic Resonance Imaging and Brain Informatics BeijingChina Key laboratory of Neurodegenerative diseases Ministry of EducationBeijingChina School of Biomedical engineering Hainan UniversityHaikouHainan ProvinceChina Center of Alzheimer’s Disease Beijing Institute for Brain DisordersBeijingChina National clinical Research Center for Geriatric Diseases BeijingChina Institute of Biomedical engineering Shenzhen Bay LaboratoryGaoke Innovation CenterShenzhenGuangdong ProvinceChina Department of Neurobiology Xuanwu HospitalCapital Medical UniversityBeijingChina

Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative ***,little is known about the long-term stability of these biomarker proteins in plasma samples stored at-80°*** aimed to explore how storage time would affect the diagnostic accuracy of these biomarkers using a large *** samples from 229 cognitively unimpaired individuals,encompassing healthy controls and those experiencing subjective cognitive decline,as well as 99 patients with cognitive impairment,comprising those with mild cognitive impairment and dementia,were acquired from the Sino Longitudinal Study on Cognitive Decline *** samples were stored at-80°C for up to 6 years before being used in this *** results showed that plasma levels of Aβ42,Aβ40,neurofilament light chain,and glial fibrillary acidic protein were not significantly correlated with sample storage ***,the level of total tau showed a negative correlation with sample storage ***,in individuals without cognitive impairment,plasma levels of total protein and tau phosphorylated protein threonine 181(p-tau181)also showed a negative correlation with sample storage *** was not observed in individuals with cognitive ***,we speculate that the diagnostic accuracy of plasma p-tau181 and the p-tau181 to total tau ratio may be influenced by sample storage ***,caution is advised when using these plasma biomarkers for the identification of neurodegenerative diseases,such as Alzheimer's ***,in cohort studies,it is important to consider the impact of storage time on the overall results.

关键词： Alzheimer’s disease amyloid-β diagnostic ability glial fibrillary acidic protein neurodegeneration neurofilament light chain plasma biomarkers single molecule array storage time tau

Effect of persistent high intraocular pressure on microstructure and hydraulic permeability of trabecular meshwork

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Chinese Physics B 2015年第5期24卷 606-613页

作者：梅曦任琳许强郑炜刘志成 School of Biomedical engineering Capital Medical University Beijing Key laboratory of Fundamental Research on Biomechanics in clinical Application shenzhen Key laboratory for molecular Imaging Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences

As the aqueous humor leaves the eye, it first passes through the trabecular meshwork （TM）. Increased flow resistance in this region causes elevation of intraocular pressure （IOP）, which leads to the occurrence of glaucoma. To quantitatively evaluate the effect of high IOP on the configuration and hydraulic permeability of the TM, second harmonic generation （SHG） microscopy was used to image the microstructures of the TM and adjacent tissues in control （normal） and high IOP conditions. Enucleated rabbit eyes were perfused at a pressure of 60 mmHg to achieve the high lOP. Through the anterior chamber of the eye, in situ images were obtained from different depths beneath the surface of the TM. Porosity and specific surface area of the TM in control and high IOP conditions were then calculated to estimate the effect of the high pressure on the permeability of tissue in different depths. We further photographed the histological sections of the TM and compared the in situ images. The following results were obtained in the control condition, where the region of depth was less than 55 μm with crossed branching beams and large pores in the superficial TM. The deeper meshwork is a silk-like tissue with abundant fluorescence separating the small size of pores. The total thickness of pathway tissues composed of TM and juxtacanalicular （JCT） is more than 100 p.m. After putting a high pressure on the inner wall of the eye, the TM region progressively collapses and decreases to be less than 40 μm. Fibers of the TM became dense, and the porosity at 34 μm in the high IOP condition is comparable to that at 105 μm in the control condition. As a consequent result, the permeability of the superficial TM decreases rapidly from 120 μm2 to 49.6 μm2 and that of deeper TM decreases from 1.66 μm2 to 0.57 μm2. Heterogeneity reflected by descent in permeability reduces from 12.4 μm of the control condition to 3.74 μm of the high IOP condition. The persistently high IOP makes the TM region colla

关键词： trabecular meshwork hydraulic permeability intraocular pressure glaucoma

AAV-mediated expression of p65shRNA and bone morphogenetic protein 4 synergistically enhances chondrocyte regeneration

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中国组织工程研究 2025年第17期29卷 3537-3547页

作者： Yu Yangyi Song Zhuoyue Lian Qiang Ding Kang Li Guangheng shenzhen Key laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration Division of Adult Joint Reconstruction and Sports MedicineDepartment of Orthopedic SurgeryShenzhen People’s Hospital(The Second Clinical Medical College Jinan UniversityThe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020Guangdong ProvinceChina Department of Orthopedics Zhengzhou Orthopedics HospitalZhengzhou 450000Henan ProvinceChina Department of Orthopedics Shenzhen Pingle Orthopedics HospitalShenzhen 518000Guangdong ProvinceChina

BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent ***,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory *** studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage ***:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis ***:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were *** efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into *** experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 *** were collected at 4,12,and 24 weeks *** staining,including safranin O and Alcian blue,was applied after collecting articular ***,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in *** AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis *** cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal *** either p65shRNA or BMP4

关键词： osteoarthritis adeno-associated virus bone morphogenetic protein 4 p65-short hairpin RNA gene therapy short hairpin RNA transforming growth factor-β1 extracellular matrix articular cartilage chondrocytes.

A FRET-Based Ratiometric H_(2)S Sensor for Sensitive Optical molecular Imaging in Second Near-Infrared Window

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Research 2024年第3期2023卷 671-684页

作者： Shan Lei Kejia Jiang Chenqing Zhang Wei Sun Yuantao Pan Dong Wang Peng Huang Jing Lin Marshall laboratory of Biomedical engineering International Cancer CenterShenzhen Key Laboratory of Tumor Visualization Molecular MedicineLaboratory of Evolutionary Theranostics(LET)School of Biomedical EngineeringShenzhen University Medical SchoolShenzhen UniversityShenzhen 518055China. Center for AIE Research College of Materials Science and EngineeringShenzhen UniversityShenzhen 518060China.

Second near-infrared(NIR-II)window optical molecular imaging kicks off a new revolution in high-quality imaging in vivo,but always suffers from the hurdles of inevitable tissue autofluorescence background and NIR-II probe ***,we prepare a Förster resonance energy transfer-based ratiometric NIR-II window hydrogen sulfide(H_(2)S)sensor through the combination of an H_(2)S-responsive NIR-II cyanine dye(acceptor,LET-1055)and an H_(2)S-inert rhodamine hybrid polymethine dye(donor,Rh930).This sensor not only exhibits high sensitivity and selectivity,but also shows rapid reaction kinetics(~20 min)and relatively low limit of detection(~96 nM)toward H_(2)S,allowing in vivo ratiometric NIR-II fluorescence imaging of orthotopic liver and colon tumors and visualization of the drug-induced hepatic H_(2)S *** findings provide the potential for advancing the feasibility of NIR-II activity-based sensing for in vivo clinical diagnosis.

关键词： H_ Infrared kinetics

A 61-mJ,1-kHz cryogenic Yb:YAG laser amplifier

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Chinese Physics B 2021年第12期30卷 437-440页

作者： Huijun He Jun Yu Wentao Zhu Qingdian Lin Xiaoyang Guo Cangtao Zhou Shuangchen Ruan shenzhen Key laboratory of Ultraintense Laser and Advanced Material Technology Center for Advanced Material Diagnostic Technologyand College of Engineering PhysicsShenzhen Technology University(SZTU)Shenzhen 518118China College of Physics and Optoelectronic engineering Shenzhen UniversityShenzhen 518060China

We report a diode-pumped rod-type Yb:YAG laser amplifier operating at 1 *** cooling method was adopted to make the Yb:YAG crystal work with four-level behavior.A single-frequency fiber laser acts as the seed in an actively Q-switched Yb:YAG *** resonator delivers 5.75-mJ pulses at 1 kHz with a pulse duration of approximately 40 *** pulses were amplified to 61 mJ in a four-pass rod-type Yb:YAG amplifier with optical-to-optical efficiency of 24%in the main *** M^(2)parameter of the output laser is<1.4.

关键词： ytterbium laser system Yb:YAG laser amplifier cryogenic laser amplification high energy laser amplification

Luminescent terpyridine-based metallo-supramolecular systems:from design to applications

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Science China Chemistry 2023年第7期66卷 1940-1962页

作者： Chenxing Guo Feng Su Pingru Su Xiujun Yu Xiaopeng Li College of Chemistry and Environmental engineering Shenzhen UniversityShenzhen 518060China Guangdong Key laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060China shenzhen University General Hospital Shenzhen University Clinical Medical AcademyShenzhen 518055China

Luminescent metallo-supramolecules have received tremendous attention in recent years owing to their diverse yet well-defined structures,tunable luminescent properties,and broad *** this context,the development of terpyridine(tpy)-based luminescent metallo-supramolecular systems has seen rapid *** review aims to summarize the recent progress of luminescent metallo-supramolecular assemblies from tpy ligands with metal ions,with a focus on discrete metallo-supramolecular architectures and metallo-supramolecular *** design strategies and common approaches for tuning the luminescence properties are detailed along with representative ***,the pitfalls and unmet challenges regarding the structural characterization and practical applications of tpy-based metallo-supramolecules are discussed followed by our perspectives on the future directions of this field.

关键词： luminescence metallo-supramolecules terpyridine coordination-driven self-assembly supramolecular chemistry

Local wavelength evolution and Landau damping of electrostatic plasma wave driven by an ultra-relativistic electron beam in dense inhomogeneous plasma

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Plasma Science and Technology 2023年第7期25卷 6-13页

作者：李然黄太武郁明阳周沧涛阮双琛 College of Physics and Optoelectronic engineering Shenzhen UniversityShenzhen 518060Peopleʼs Republic of China shenzhen Key laboratory of Ultraintense Laser and Advanced Material Technology Center for Advanced Material Diagnostic Technologyand College of Engineering PhysicsShenzhen Technology UniversityShenzhen 518118Peopleʼs Republic of China

Evolution of an electrostatic plasma wave driven by a low-density ultra-relativistic electron beam in dense inhomogeneous plasma is considered. In particular, the wavelength variation as observed at fixed locations in the plasma is analyzed in terms of the wave characteristics. It is shown that for a negative density gradient, the observed local wavelength decreases monotonically with time, but for a positive density gradient, it first increases and then decreases with time, accompanied by reversal of the wave phase. However, in both cases the local wavelength eventually decreases with time since Landau damping becomes significant as the wavelength becomes of the order of the plasma Debye length. Results from particle-in-cell simulations agree well with theoretical analyses of the wavelength variation.

关键词： relativistic electron beam transport inhomogeneous plasma energy dissipation

Acceptor-engineering tailored type-Ⅰ photosensitizer with aggregation-induced NIR-II emission for cancer multimodal phototheranostics

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Science China Chemistry 2024年第8期67卷 2647-2660页

作者： Jiangao Li Niu Niu Deliang Wang Xue Liu Yi Qin Lei Wang Ben Zhong Tang Dong Wang Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhen 518060China Guangzhou Municipal and Guangdong Provincial Key laboratory of molecular Target Clinical Pharmacologythe State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China School of Science and engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhen(CUHK-Shenzhen)Shenzhen 518172China

Exploration of single molecular species synchronously featured by long excitation/emission wavelength, accurate diagnosis, and effective therapy, remains supremely appealing to implement high-performance cancer phototheranostics. However, those previously established phototheranostic agents are undiversified and stereotyped in terms of structural skeleton, and generally exhibit insufficient phototheranostic outcomes. Herein, we innovatively utilized indanone-condensed thiadiazolo[3,4-g]quinoxaline(ITQ) as electron acceptor to construct novel photosensitizer with second near-infrared(NIR-II) emission. Experimental study and theoretical calculation demonstrated that comparing with the counterparts constituting by widely employed NIR-II building block benzobisthiadiazole(BBTD) and 6,7-diphenylthiadiazoloquinoxaline(DPTQ), ITQ-based photosensitizer(TITQ) showed superior aggregation-induced emission(AIE) characteristics, much stronger type-I reactive oxygen species(ROS) production, and prominent photothermal conversion capacity. Furthermore, TITQ nanoparticles with excellent biocompatibility were capable of effectively accumulating in the tumor site and visualizing tumor through fluorescence-photoacoustic-photothermal trimodal imaging with highly spatiotemporal resolution, and completely eliminating tumor by type-I photodynamic-photothermal therapy.

关键词： phototheranostics aggregation-induced emission NIR-Ⅱfluorescence acceptor engineering photodynamic-photothermal therapy

Proton acceleration from picosecond-laser interaction with a hydrocarbon target

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Plasma Science and Technology 2023年第2期25卷 77-85页

作者：杨宇晨黄太武蒋轲郁明阳周沧涛 College of Applied Sciences Shenzhen UniversityShenzhen 518060People's Republic of China shenzhen Key laboratory of Ultraintense Laser and Advanced Material Technology Center for Advanced Material Diagnostic Technologyand College of Engineering PhysicsShenzhen Technology UniversityShenzhen 518118People's Republic of China

As an intense picosecond laser pulse irradiates a hydrocarbon target,the protons therein can be accelerated by the radiation pressure as well as the sheath field behind the *** investigate the effect of the laser and hydrocarbon target parameters on proton acceleration with two/threedimensional particle-in-cell *** is found that the resulting two-ion species plasma can generate a multiple peaked charge-separation field that accelerates the *** particular,a smaller carbon-to-hydrogen ratio,as well as the thinner and/or lower density of the target,leads to a larger sheath field and thus proton beams with a larger cutoff energy and smoother energy *** results may be useful in achieving high-flux quasi-monoenergetic proton beams by properly designing the hydrocarbon target.

关键词： picosecond laser pulse proton acceleration multiple peaked charge-separation field carbon-to-hydrogen ratio