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Nucleic acids induced peptide-based AIE nanoparticles for fast cell imaging

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Chinese Chemical Letters 2021年第4期32卷 1571-1574页

作者： Wenjun Li Yaping Zhang Yanchao Wang Yue Ma Dongyuan Wang Heng Li Xiyang Ye Feng Yin Zigang Li State Key Laboratory of Chemical Onco.enomics School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhen 518055China Pingshan Translational Medicine Center Shenzhen Bay LaboratoryShenzhen 518055China shenzhen Youwei Technology Holdings Group co. LtdShenzhen 518055China Department of Chemistry Tsinghua University Shenzhen International Graduate SchoolShenzhen 518055China Department of Gyneco.ogy.shenzhen People's Hospital Shenzhen 518020China

Herein,we utilized nucleic acids induced peptide co.assembly strategy to develop novel nucleic acids induced peptide-based AIE(NIP-AIE)*** fluorescent of AIE co.ld be observed when a little amount of nucleic acids was added into the peptide solution,and the intensity co.ld be regulated by the co.centration of nucleic *** AIE nanoparticle with good bioco.patibility co.ld achieve fast cell *** is also proved that the fluorescence intensity of AIE decreased with time,which indicates that the reducible cross-linkers of Wpc peptide by GSH and nanoparticles gradually disintegrate in *** on the different of AIE fluorescence signals which regulated by the formation and disintegration of nanoparticles,this AIE system is expected to be used for real-time monitoring of drug release from peptide-based nano carriers in vivo or in vitro,and may provide a new platform for the co.struction of other organic AIE nanoparticles.

关键词： Peptide-based AIE nanoparticles Nucleic acids Cell imaging

Tyroserleutide tripeptide affects calcium homeostasis of human hepatocarcinoma BEL-7402 cells

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Science China(Life Sciences) 2005年第5期48卷 523-530页

作者： FU Zheng1, LU Rong2, LI Guoli1, ZHAO Lan1, GAO Weizhen3, CHE Xuchun1, JIAN Xu4, ZHOU Chunlei1 & YAO Zhi1 1. Department of Immunology, Tianjin Medical University, Tianjin 300070, China 2. shenzhen kangzhe Pharmaceutical co. Ltd., shenzhen, 518029, China 3. Department of Pharmacy, Tianjin Medical University, Tianjin 300070, China 4. Central Laboratory of Tianjin Medical University Hospital, Tianjin 300070, China 1. Department of Immunology Tianjin Medical University 300070 Tianjin China 2. shenzhen kangzhe Pharmaceutical co. Ltd. 518029 Shenzhen China 3. Department of Pharmacy Tianjin Medical University 300070 Tianjin China 4. Central Laboratory of Tianjin Medical University Hospital 300070 Tianjin China

This study aimed to observe the effects of tyroserleutide (tyrosyl-seryl-leucine, YSL) on the growth of human hepatocarcinoma BEL-7402 that was transplanted into nude mice, and explore its anti-tumor mechanism preliminarily. YSL, at doses of 80 μg·kg?1·d?1, 160 μg·kg?1·d?1 and 320 μg·kg?1·d?1 significantly inhibited the growth of the human hepatocarcinoma BEL-7402 tumor in nude mice, producing inhibition of 21.66%, 41.34%, and 34.78%, respectively. Ultra structure of BEL-7402 tumor in nude mice showed that YSL co.ld induce tumor cells apoptosis and necrosis, cell organelle mitochondria and endoplasmic reticulum damage, and calcium over-load. By co.focal laser scanning microsco.y and flow cytometry, we found that 10 μg/mL YSL rapidly induced an increase of the co.centration of cytoplasmic free calcium in BEL-7402 cells in vitro, and maintained high co.centrations of cytoplasmic free calcium for 1 h. Then the calcium co.centration began to decrease after 2 h, and was lower than that of the co.trol group at 4 h and 24 h (p<0.05). YSL also decreased the mitochondrial transmembrane potential of BEL-7402 cells in vitro, but had no effect on the calcium homeostasis or mitochondrial transmembrane potential of Chang liver hepatocytes. So affecting calcium homeostasis, then inducing apoptosis and necrosis may be a mechanism by which YSL inhibits the tumor growth in animal model.

关键词： tyroserleutide, YSL, human hepatocarcinoma BEL-7402, cytoplasmic free calcium, mitochondrial transmembrane potential.

Anti-hepatitis B virus activities of natural products and their antiviral mechanisms

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Chinese Journal of Natural Medicines 2023年第11期21卷 803-811页

作者： DENG Wanyu CHEN Fu ZHAO Yue ZHOU Ming GUO Min co.lege of Life Science Shangrao Normal UniversityShangrao 334001China BGI-shenzhen Shenzhen 518000China shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518110China Liver-biotechnology(shenzhen)co. Ltd.Shenzhen 518110China State Key Laboratory of Natural Medicines School of Life Science&TechnologyChina Pharmaceutical UniversityNanjing 211198China

Chronic hepatitis B (CHB) infections caused by the hepatitis B virus (HBV) co.tinue to pose a significant global public health challenge. Currently, the approved treatments for CHB are limited to interferon and nucleos(t)ide analogs, both of which have their limitations, and achieving a co.plete cure remains an elusive goal. Therefore, the identification of new therapeutic targets and the development of novel antiviral strategies are of utmost importance. Natural products (NPs) co.stitute a class of substances known for their diverse chemical structures, wide-ranging biological activities, and low toxicity profiles. They have shown promise as potential candidates for co.bating various diseases, with a substantial number demonstrating anti-HBV properties. This co.prehensive review focuses on the current applications of NPs in the fight against HBV and provides a summary of their antiviral mechanisms, co.sidering their impact on the viral life cycle and host hepatocytes. By offering insights into the world of anti-HBV NPs, this review aims to furnish valuable information to support the future development of antiviral drugs.

关键词： Hepatitis B virus Natural products Antiviral stategy Mechanisms Host hepatocyte Life cycle

A fast automatic reco.nition and location algorithm for fetal genital organs in ultrasound images

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2009年第9期10卷 648-658页

作者： Sheng TANG Si-ping CHEN Post-Doctoral Research Station Shenzhen University Shenzhen 518060 China post-Doctoral WorkStation Microprofit Electronie Co. Ltd Shenzhen 518057 China Department of Biomedical Engineering Shenzhen University Shenzhen 518060 China

Severe sex ratio imbalance at birth is now beco.ing an important issue in several Asian co.ntries. Its leading immediate cause is prenatal sex-selective abortion following illegal sex identification by ultrasound scanning. In this paper, a fast automatic reco.nition and location algorithm for fetal genital organs is proposed as an effective method to help prevent ultrasound technicians from unethically and illegally identifying the sex of the fetus. This automatic reco.nition algorithm can be divided into two stages. In the 'rough' stage, a few pixels in the image, which are likely to represent the genital organs, are automatically chosen as points of interest (POIs) acco.ding to certain salient characteristics of fetal genital organs. In the 'fine' stage, a specifically supervised learning framework, which fuses an effective feature data preprocessing mechanism into the multiple classifier architecture, is applied to every POI. The basic classifiers in the framework are selected from three widely used classifiers: radial basis function network, backpropagation network, and support vector machine. The classification results of all the POIs are then synthesized to determine whether the fetal genital organ is present in the image, and to locate the genital organ within the positive image. Experiments were designed and carried out based on an image dataset co.prising 658 positive images (images with fetal genital organs) and 500 negative images (images without fetal genital organs). The experimental results showed true positive (TP) and true negative (TN) results from 80.5% (265 from 329) and 83.0% (415 from 500) of samples, respectively. The average co.putation time was 453 ms per image.

关键词： Ultrasound image Fetal genital organ Point of interest (POI) Feature selection Feature extraction Class imbalance Multiple classifier architecture

Dual stimulus responsive borosilicate glass(BSG)scaffolds promote diabetic alveolar bone defectsrepair by modulating macrophage phenotype

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Bioactive Materials 2023年第8期26卷 231-248页

作者： Pengfei Tian Limin Zhao Jua Kim Xian Li Chunyu Liu Xu Cui Tao Liang Yunbo Du Xiehui Chen Haobo Pan Center for Human Tissues and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055PR China shenzhen Longhua District Central Hospital Shenzhen518000PR China shenzhen Healthemes biotechnology co.Ltd Shenzhen518102PR China

The regeneration of alveolar bone is still clinical challenge,particularly acco.panied with diabetes,causing metabolic disorder with a protracted low-grade inflammatory *** a result,the anticipated loading of biomaterials is highly suspicious in spontaneous modulation of cells function,which is mostly disturbed by co.stant *** this study,we developed gluco.e and hydrogen peroxide dual-responsive borosilicate glass(BSG)scaffolds loaded with epigallocatechin gallate(EGCG)to synergistically modulate the abnormal inflammation of diabetic alveolar bone *** was found that the release of EGCG by BSG co.ld directly regulate the shift of macrophages from M1 to the M2 phenotype by promoting autophagy and lessening the inhibition of autophagic ***,EGCG can also indirectly regulate the polarization phenotype of macrophages by reducing the activation of NF-κb in stem cells and restoring its immunoregulatory ***,the addition of EGCG to BSG scaffold in diabetes allows for a more striking modulation of the macrophage phenotype in a timely *** altered macrophage phenotype reduces local inflammation and thus increases the ability to repair diabetic alveolar bone,showing promise for the treatment of alveolar defect in diabetic patients.

关键词： Borosilicate Epigallocatechin gallate(EGCG) Alveolar bone Autophagy Mitochondria Macrophage immunomodulatory Diabetes

Small-molecule amines:a big role in the regulation of bone homeostasis

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Bone Research 2023年第3期11卷 457-480页

作者： Qian Zhang Jirong Yang Nan Hu Juan Liu Huan Yu Haobo Pan Di Chen Changshun Ruan Research Center for Human Tissue and Organs Degeneration Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China Department of Nephrology Shenzhen People’s Hospital(The Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020 GuangdongChina Research Center for co.puter-Aided Drug Disco.ery Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China shenzhen Healthemes biotechnology co. Ltd.Shenzhen 518102China Faculty of Pharmaceutical Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Numerous small-molecule amines(SMAs)play critical roles in maintaining bone homeostasis and promoting bone regeneration regardless of whether they are applied as drugs or *** the one hand,SMAs promote bone formation or inhibit bone resorption through the regulation of key molecular signaling pathways in osteoblasts/osteoclasts;on the other hand,owing to their alkaline properties as well as their antioxidant and anti-inflammatory features,most SMAs create a favorable microenvironment for bone ***,due to a lack of information on their structure/bioactivity and underlying mechanisms of action,certain SMAs cannot be developed into drugs or biomaterials for bone disease *** this review,we thoroughly summarize the current understanding of SMA effects on bone homeostasis,including descriptions of their classifications,biochemical features,recent research advances in bone biology and related regulatory mechanisms in bone *** addition,we discuss the challenges and prospects of SMA translational research.

关键词： amines biomaterials alkaline

Optimal Placement of Phasor Measurement Unit in Smart Grids co.sidering Multiple co.straints

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Journal of Modern Power Systems and Clean Energy 2023年第2期11卷 479-488页

作者： Tengpeng Chen He Ren Yuhao Sun Markus Kraft Gehan A.J.Amaratunga Department of Instrumental and Electrical Engineering Xiamen UniversityXiamenChina shenzhen Research Institute of Xiamen University ShenzhenChina CTC Intelligence(shenzhen)Technology co. Ltd.ShenzhenChina Department of Chemical Engineering and biotechnology University of CambridgeCambridgeUK Cambridge Centre for Advanced Research and Education in Singapore CREATE TowerSingapore School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore Department of Engineering University of CambridgeUK

The distribution of measurement noise is usually assumed to be Gaussian in the optimal phasor measurement unit(PMU)placement(OPP)***,this is not always accurate in *** paper proposes a new OPP method for smart grids in which the effects of co.ventional measurements,limited channels of PMUs,zero-injection buses(ZIBs),single PMU loss co.tingency,state estimation error(SEE),and the maximum SEE variance(MSEEV)are *** SEE and MSEEV are both obtained using a robust t-distribution maximum likelihood estimator(MLE)because t-distribution is more flexible for modeling both Gaussian and non-Gaussian *** A-and G-optimal experimental criteria are utilized to form the SEE and MSEEV *** allows the optimization problem to be co.verted into a linear objective function subject to linear matrix inequality observability *** performance of the proposed OPP method is verified by the simulations of the IEEE 14-bus,30-bus,and 118-bus systems as well as the 211-bus practical distribution system in China.

关键词： Phasor measurement unit(PMU) smart grids optimal placement state estimation(SE).

Clean Monascus pigments production from Chinese rice wine wastes through submerged fermentation

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Food Bioscience 2023年第2期52卷 843-850页

作者： Mengfei Long Yongchao Cai Nan Zheng Zhi Lu Wentao Cao Yaxian Li Xiaomei Pei Osire Tolbert Xiaole Xia The Key Laboratory of Industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China Infinitus(China)co.Ltd. Guangzhou510665China Faculty of Biology Shenzhen MSU-BIT UniversityShenzhen518172China

The potential for Monascus pigments(MPs)production in a mixture of Chinese rice wine lees,soaked rice wastewater and broken rice was *** production reached 120.1 U/mL with optimal solid-liquid ratio of culture medium 1:10 and 20 g/L broken rice addition at shake flask *** further explored the effects of stirring speed on MPs production in 7.5 L agitated *** stirring speed had a great impact on cell morphology and the maximum MPs co.centration and yield were 128.4 U/mL and 8174 U/g respectively at 400 *** soluble chemical oxygen demand for the soaked rice wastewater decreased by 78.7%,which was mainly due to the intake of carbohydrates,organic acids and amino ***,a simultaneous fermentation process and production system with two-stage stirring speed co.trol strategy was further developed in the 50 L pilot scale,in which the MPs production reached 158.9 U/***,Chinese rice wine wastes were fully utilized without addition of organic solvents during the process and no generation of new *** results showed that Monascus was capable of co.verting Chinese rice wine wastes into natural pigments by submerged fermentation,which co.ld provide an eco.omic and environmentally friendly approach to reduce the co.t of wastes treatment as well as high-value utilization.

关键词： Monascus pigments Submerged fermentation Chinese rice wine wastes Pilot scale fermentation Simultaneous fermentation process and production system

Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay

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Food Quality and Safety 2022年第4期6卷 597-607页

作者： Jiaxu Xiao Hongfei Yang Liangni Qin Jixiang Liang Long Li Xiaohui Fan Dapeng Peng National Reference Laboratory of Veterinary Drug Residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug Residues in Foods Huazhong Agricultural UniversityWuhanChina Wuhan Shangcheng biotechnology co. Ltd.WuhanChina shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhenChina shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human ***,this study aims to develop a co.loidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on *** and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against *** using a strategy of heterologous co.ting antigen,different co.ting antigens are applied to detect *** gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and ***:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:*** co.bination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting co.centration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A co.parative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs ***:The results indicated that the test strip can realize full co.erage reco.nition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.

关键词： fluoroquinolones co.loidal gold microwell purification residues