Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay
作者机构：National Reference Laboratory of Veterinary Drug Residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug Residues in FoodsHuazhong Agricultural UniversityWuhanChina Wuhan Shangcheng Biotechnology Co.Ltd.WuhanChina Shenzhen Institute of Nutrition and HealthHuazhong Agricultural UniversityShenzhenChina Shenzhen BranchGuangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina
出 版 物：《Food Quality and Safety》 (食品品质与安全研究（英文版）)
年 卷 期：2022年第6卷第4期
页 面：597-607页
核心收录：
学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 07[理学] 071002[理学-动物学]
基 金：funded by the Key Research and Development Program of Hubei Province(CN)(No.2020BBB079) the Fundamental Research Funds for the Central Universities(2662022DKPY007) the HZAU-AGIS Cooperation Fund(SZYJY2022024),China.
主 题：fluoroquinolones colloidal gold microwell purification residues
摘 要：Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human health.Here,this study aims to develop a colloidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on site.Materials and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against FQs.By using a strategy of heterologous coating antigen,different coating antigens are applied to detect FQs.The gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and antibody.Results:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:1024000.The combination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting concentration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A comparative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs residues.Conclusions:The results indicated that the test strip can realize full coverage recognition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.
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