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Virologica Sinica 2022年第6期37卷 946-949页

作者： Zhiyun Yin Fuli Ren Min Zhou Shengyao Chen Yali Deng Sijing Hu Fei Deng Shu Shen Junming Shi National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan430071China Academic Affairs Office of Cangzhou Jiaotong University Cangzhou061199China Center for Translational Medicine Jinyintan HospitalWuhan430023China

Dear Editor,The severe fever with thrombocytopenia syndrome virus(SFTSV) is an emerging tick-borne pathogen belonging to the genus Bandavirus,family Phenuiviridae, which causes high fatality rates ranging from 12%to 30%(Kim et al., 2013). In the absence of efficacious drugs and vaccines, therapies for SFTS are performed mostly according to the clinical manifestations accompanied by supportive treatment. The SFTSV polymerase protein(SFTSV-L), encoded by its L segment, plays a critical role in genomic replication and transcription.

关键词： fever drugs residues

Carposome productivity of Pleurotus ostreatus and Pleurotus eryngii growing on agro-industrial residues enriched with nitrogen,calcium salts and oils

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Carbon Resources Conversion 2023年第2期6卷 150-165页

作者： Marianna Dedousi Eirini-Maria Melanouri Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural Products(ITAP)Hellenic Agricultural Organization-Dimitra1 S.Venizelou Str14123 LykovryssiGreece Laboratory of Food Microbiology and Biotechnology Department of Food Science and NutritionAgricultural University of Athens75 Iera Odos Str11855 AthensGreece

The suitability of the abundant agro-industrial residues wheat straw(WS;control),barley and oats straw(BOS)and rice husk(RH),supplemented with various sources of oils(sunflower,corn oil),nitrogen(peptone,yeast extract)and calcium salts(CaSO_(4)⋅2H_(2)O,CaCl2),as novel substrates in solid-state fermentation of selected Pleurotus ostreatus and P.eryngii mushrooms was investigated.The possible effect of different additives on mycelial growth rate,biomass production and endoglucanase,laccase and lipase biosynthesis were evaluated.Moreover,their impact on essential cultivation aspects(earliness,total mushroom yield,biological efficiency)and carposome quality parameters(weight,morphological characteristics)was assessed.Both fungi showed their highest growth rates on BOS substrates and the most positive implementation was CaSO_(4)⋅2H_(2)O 6%w/w(Kr=9.58 mm/day;P.ostreatus,Kr=9.42 mm/day;P.eryngii),while different additives led to enhancement of biomass production.Pleurotus species demonstrated minimal levels of endoglucanase activity,with values ranging from 0.01 to 0.42 U/g of dry weight,regardless of the substrate and the stage of colonization.On the contrary,the maximum values of laccase activity were observed at 50%of colonization on BOS and RH,while supplementation with nitrogen and calcium sources positively affected its biosynthesis.P.ostreatus and P.eryngii cultivated on BOS supplemented with peptone at 2 and 5%w/w,synthesized significant laccase amounts,i.e.,12,165.78 and 8,624.55 U/g d.w.,respectively.Satisfactory amounts of lipase were produced,especially in substrates supplemented with sunflower 2%w/w,in quantities up to 1.42 U/g d.w.,whereas the highest lipase activity was achieved by P.eryngii on WS supplemented with corn oil at 2%w/w,with a value of 4.25 U/g d.***.being recorded.Regarding fermentation of Pleurotus species in polypropylene bags,WS and BOS supported faster colonization and shorter earliness period than RH substrates,whereas supplementation did not seem to affect these culture parameters.Furthe

关键词： Oyster mushrooms residues Additives Biomass Endoglucanase Laccase Lipase Weight

Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay

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Food Quality and Safety 2022年第4期6卷 597-607页

作者： Jiaxu Xiao Hongfei Yang Liangni Qin Jixiang Liang Long Li Xiaohui Fan Dapeng Peng National Reference Laboratory of Veterinary Drug residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug residues in Foods Huazhong Agricultural UniversityWuhanChina Wuhan Shangcheng Biotechnology Co. Ltd.WuhanChina Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhenChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human health.Here,this study aims to develop a colloidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on site.Materials and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against FQs.By using a strategy of heterologous coating antigen,different coating antigens are applied to detect FQs.The gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and antibody.Results:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:1024000.The combination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting concentration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A comparative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs residues.Conclusions:The results indicated that the test strip can realize full coverage recognition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.

关键词： fluoroquinolones colloidal gold microwell purification residues

Physical and mechanical properties of municipal solid waste incineration residues with cement and coal fly ash using X-ray Computed Tomography scanners

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Frontiers of Structural and Civil Engineering 2019年第3期13卷 640-652页

作者： Toshifumi MUKUNOKI Ta Thi HOAI Daisuke FUKUSHIMA Teppei KOMIYA Takayuki SHIMAOKA Faculty of Advanced Science and Technology Graduate School of Kumamoto UniversityKumamoto city 860-8555Japan Graduate school of Science and Technology Kumamoto UniversityKumamoto city 860-8555Japan Faculty of Geology VNU University of ScienceVietnam National University Hanoi334 Nguyen TraiHanoiVietnam Water and Material Cycles System Faculty of EngineeringKyushu UniversityFukuoka city 819-0395Japan

A significant volume of Municipal Solid Waste incineration bottom ash and fly ash (i.e.,incineration residues) are commonly disposed as landfill.Meanwhile,reclamation of landfill sites to create a new land space after their closure becomes an important goal in the current fewer and fewer land availability scenario in many narrow countries.The objective of this study is to reclaim incineration residue materials in the landfill site by using cement and coal fly ash as stabilizers aiming at performing quality check as new developed materials before future construction.Indeed,physical and mechanical properties of these new materials should be initially examined at the micro scale,which is the primary fundamental for construction at larger scale.This research examines quantitative influences of using the combination of cement and coal fly ash at different ratio on the internal structure and ability of strength enhancement of incineration residues when suffering from loading.Couple of industrial and micro-focus X-ray computed tomography (CT) scanners combined with an image analysis technique were utilized to characterize and visualize the behavior and internal structure of the incineration residues-cement-coal fly ash mixture under the series of unconfined compression test and curing period effect.Nine types of cement solidified incineration residues in term of different curing period (i.e.,7,14,28 days) and coal fly ash addition content (i.e.,0%,9%,18%) were scanned before and after unconfined compression tests.It was shown that incineration residues solidified by cement and coal fly ash showed an increase in compression strength and deformation modulus with curing time and coal fly ash content.Three-dimension computed tomography images observation and analysis confirmed that solidified incineration residues including incineration bottom and fly ash as well as cement and coal fly ash have the deliquescent materials.Then,it was studied that stabilized parts play a more important role than spatial void distribution in i

关键词： mechanical property municipal solid waste incineration residues coal fly ash unconfined compression test image analysis X-Ray Computed Tomography scanners

Functional diversity of subgroup 5 R2R3-MYBs promoting proanthocyanidin biosynthesis and their key residues and motifs in tea plant

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Horticulture Research 2023年第8期10卷 216-230页

作者： Tianming Jiao Yipeng Huang Ying-Ling Wu Ting Jiang Tongtong Li Yanzhuo Liu Yvchen Liu Yunyun Han Yajun Liu Xiaolan Jiang Liping Gao Tao Xia State Key Laboratory of Tea Plant Biology and Utilization/Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture/Anhui Provincial Laboratory of Tea Plant Biology and Utilization Anhui Agricultural UniversityWest 130 Changjiang RoadHefei 230036 AnhuiChina School of Life Science Anhui Agricultural UniversityHefei 230036AnhuiChina

The tea plant(Camellia sinensis)is rich in polyphenolic compounds.Particularly,flavan-3-ols and proanthocyanidins(PAs)are essential for the flavor and disease-resistance property of tea leaves.The fifth subgroup of R2R3-MYB transcription factors comprises the primary activators of PA biosynthesis.This study showed that subgroup 5 R2R3-MYBs in tea plants contained at least nine genes belonging to the TT2,MYB5,and MYBPA types.Tannin-rich plants showed an expansion in the number of subgroup 5 R2R3-MYB genes compared with other dicotyledonous and monocot plants.The MYBPA-type genes of tea plant were slightly expanded.qRT–PCR analysis and GUS staining analysis of promoter activity under a series of treatments revealed the differential responses of CsMYB5s to biotic and abiotic stresses.In particular,CsMYB5a,CsMYB5b,and CsMYB5e responded to high-intensity light,high temperature,MeJA,and mechanical wounding,whereas CsMYB5f and CsMYB5g were only induced by wounding.Three genetic transformation systems(C.sinensis,Nicotiana tabacum,and Arabidopsis thaliana)were used to verify the biological function of CsMYB5s.The results show that CsMYB5a,CsMYB5b,and CsMYB5e could promote the gene expression of CsLAR and CsANR.However,CsMYB5f and CsMYB5g could only upregulate the gene expression of CsLAR but not CsANR.A series of site-directed mutation and domain-swapping experiments were used to verify functional domains and key amino acids of CsMYB5s responsible for the regulation of PA biosynthesis.This study aimed to provide insight into the induced expression and functional diversity model of PA biosynthesis regulation in tea plants.

关键词： plant wound residues

Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

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Natural Products and Bioprospecting 2016年第4期6卷 195-204页

作者： Caroline Alves Breda Alessandra Marcon Gasperini Vera Lucia Garcia Karin Maia Monteiro Giovana Anceski Bataglion Marcos Nogueira Eberlin Marta Cristina Teixeira Duarte School of Food Engineering State University of CampinasUNICAMPMonteiro Lobato Street80BaraōGeraldoCampinasSaōPaulo 13083-862Brazil Microbiology Division of Research Center for Chemistry Biology and Agriculture–CPQBAUniversity of CampinasUNICAMPAlexandre Cazelatto Street999BetelPaulı´niaSaöPaulo 13148-218Brazil Organic Chemistry and Pharmaceutical Division of Research Center for Chemistry Biology and Agriculture–CPQBAUniversity of CampinasUNICAMPAlexandre Cazelatto Street999BetelPaulíniaSaöPaulo 13148-218Brazil Pharmacology and Toxicology Division of Research Center for Chemistry Biology and Agriculture–CPQBAUniversity of CampinasUNICAMPAlexandre Cazelatto Street999BetelPaulíniaSaöPaulo 13148-218Brazil ThoMSon Mass Spectrometry Laboratory Institute of ChemistryState University of CampinasUNICAMPCampinasSaōPaulo 13084-971Brazil

The increasing demand for safe food without preservatives or pesticides residues has encouraged several studies on natural products with antifungal activity and low toxicity.In this study,ethanolic extracts from leaves and fruit residues(peel and seeds)of three Brazilian savanna species(Acrocomia aculeata,Campomanesia adamantium and Caryocar brasiliense)were evaluated against phytopathogenic fungi.Additionally,the most active extract was chemically characterized by ESI-MS and its oral acute toxicity was evaluated.Extracts from C.brasiliense(pequi)peel and leaves were active against Alternaria alternata,Alternaria solani and Venturia pirina with minimal inhibitory concentrations between 350 and 1000 lg/mL.When incorporated in solid media,these extracts extended the lag phase of A.alternata and A.solani and reduced the growth rate of A.solani.Pequi peel extract showed better antifungal activity and their ESI-MS analysis revealed the presence of substances widely reported as antifungal such as gallic acid,quinic acid,ellagic acid,glucogalin and corilagin.The oral acute toxicity was relatively low,being considered safe for use as a potential natural fungicide.

关键词： Brazilian savanna fruits residues Natural fungicides Phytopathogens

Plant residues differing in C/N ratio in mulch and soil-the effect of the mulch on nutrient availability and microbial biomass is more pronounced with higher leaching amount

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Soil Ecology Letters 2020年第4期2卷 317-326页

作者： Thi Hoang Ha Truong Petra Marschner School of Agriculture Food and WineThe University of AdelaideSouth Australia5005Australia Quang Binh University Dong Hoi CityQuang Binh ProvinceVietnam

This study investigated the effect of C/N ratio,placement of plant residues,and leaching amounts on soil respiration,microbial biomass,and nutrient availability within four weeks after amendment.Young faba bean shoots(FB,C/N 7)and mature wheat straw(WH,C/N 80)were used as low and high C/N residue,respectively.Soil was unamended,mulched with FB or WH only,or mulched with one residue and mixed with the other residue.Leaching with 5 or 25 mL water was carried out on days 4,12,and 20.Cumulative respiration and microbial biomass N were higher with 25 than 5 mL only in treatments with two residues.WH under FB mulch reduced N availability compared to FB mulch alone,whereas FB under WH increased N availability compared toWHmulch alone.When soils were leached with 25 mL water,available N in FB mulch over WH was lower on day 12,but higher later,compared to WH mulch over FB.In contrast,in WH mulch over FB microbial biomass N increased over time whereas available N decreased.In conclusion,the effect of C/N ratio of the mulch on soil available and microbial biomass N was greater with the higher leaching amount.

关键词： Leaching amount Mulching residues C/N ratio Microbial biomass N N availability

茶叶浸出液中农药残留表面拉曼光谱检测与初步定量分析

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分析试验室 2020年第2期39卷 148-153页

作者：许丽梅赖国银康怀志厦门华厦学院环境与公共健康学院厦门361024 厦门市出入境检验检疫局技术中心厦门361026 厦门大学萨本栋微米纳米科学技术研究院厦门3610005

选取甲基对硫磷和水胺硫磷为研究对象,改良了传统的QuEChERS前处理工艺,以自制纳米金溶胶为增强基底,利用表面增强拉曼光谱(SERS)技术,对茶叶浸出液中的农药残留进行检测。通过比对两种有机磷农药的拉曼特征峰进行定性分析。同时,选取57... 详细信息

选取甲基对硫磷和水胺硫磷为研究对象,改良了传统的QuEChERS前处理工艺,以自制纳米金溶胶为增强基底,利用表面增强拉曼光谱(SERS)技术,对茶叶浸出液中的农药残留进行检测。通过比对两种有机磷农药的拉曼特征峰进行定性分析。同时,选取570,1034,1107和1202 cm^-1等拉曼位移附近的特征峰光谱数据,利用微分等数学手段,结合偏最小二乘法(PLSR)建立回归方程,预测样品中农药残留含量。所得预测数值与气相色谱-质谱联用(GC-MS)法检测值对比,验证本方法的可行性与可信度。结果表明:基于SERS技术对上述两种有机磷农药的检出限可达0.05 mg/L;通过数学模型分析建立回归方程,其线性相关系数范围为0.9077~0.9824,预测均方根误差(RMSEP)范围为0.77%~2.68%;利用回归方程得到的预测值与GC-MS检测结果基本接近,相对误差范围-5.16%~9.03%,回收率为81.4%~115.1%,说明可以用SERS技术对茶叶浸出液中的有机磷农药残留进行定性和初步定量分析。

关键词： surface enhanced Raman spectroscopy pesticide residues quantitative analysis partial least squares regression QuEChERS

A SARS-CoV-2 antibody retains potent neutralization against Omicron by targeting conserved RBM residues

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Cellular & Molecular Immunology 2022年第5期19卷 647-649页

作者： Chunyan Yi Zhiyang Ling Xiao Lu Yadong Fu Zhuo Yang Sonam Wangmo Shuangfeng Chen Yaguang Zhang Liyan Ma Wangpeng Gu Hongzhou Lu Xiaoyu Sun Bing Sun State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai200031China School of Life Science and Technology ShanghaiTech UniversityShanghai201210China National Clinical Center for Infectious Disease The Third People’s Hospital of Shenzhen and the Second Affiliated Hospital of Southern University of Science and TechnologyShenzhen518112Guangdong ProvinceChina Shanghai Public Health Clinical Center Shanghai Medical CollegeFudan UniversityShanghai201508China

The newly emerged Omicron(B.1.1.529)variant of SARS-CoV-2 is quickly overtaking the Delta variant and becoming the dominant strain around the world due to its enhanced transmissibility and high immune escape potential[1,2].Compared to the Wuhan strain,the Omicron variant carries 37 spike mutations,of which 15 are within the receptor-binding domain(RBD)(Fig.1A).A high mutation rate is associated with remarkable resistance to current vaccines and RBD-specific antibody therapeutics[2,3,4].

关键词： residues becoming Omi

Mutation of the critical pH-gating residues histidine 231 to glutamate increase open probability of outer membrane protein G in planar lipid bilayer

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Protein & Cell 2013年第11期4卷 803-806页

作者： Mu Yu Peibei Sun Yao He Liang Xiao Demeng Sun Longhua Zhang Changlin Tian High Magnetic Field Laboratory Chinese Academic of SciencesHefei 230031China Hefei National Laboratory for Physical Science at Microscale and School of Life Science University of Science and Technology of ChinaHefei 230027China

Dear Editor,Outer-membrane protein G(OmpG)is a nonspecificβ-barrel porin in the outer membrane of Escherichia coli(E.coli),allowing the passage of ions and molecules up to 900 Da(Fajardo et al.,1998).It comprises of 280 amino acids that form 14-strandedβ-sheets with seven long loops(L1-L7)on the extra-cellular side and six short turns on the periplasmic side(Subbarao and van den Berg,2006;Yildiz et al.,2006;Liang and Tamm,2007).Despite that the OmpG gene exists in the genome of several E.coli strains(Nikaido,1999),expres-sion of OmpG was only observed in E.coli mutants lacking OmpF and LamB(Fajardo et al.,1998)to enable the diffu-sion of maltodextrins across the bacte-rial outer membrane.Very interestingly,unlike usual trimeric channel-forming porins,OmpG exhibits fascinating characteristics of a functional monomer in physiological and structural studies(Conlan and Bayley,2003;Mari et al.,2010).

关键词： outer planar residues