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Integrated metabolism and epigenetic modifications in the macrophages of mice in responses to cold stress

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第6期23卷 461-480页

作者： Jingjing LU Shoupeng FU Jie DAI Jianwen HU Shize LI Hong JI Zhiquan WANG Jiahong YU Jiming BAO Bin XU Jingru GUO Huanmin YANG college of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityDaqing163319China college of Veterinary Medicine Jilin UniversityChangchun130062China shanghai bioprofile co.ltd. Shanghai201100China Faculty of Agricultural Life and Environmental SciencesUniversity of AlbertaEdmontonAlbertaT5J 4P6Canada

The negative effects of low temperature can readily induce a variety of diseases.We sought to understand the reasons why cold stress induces disease by studying the mechanisms of fine-tuning in macrophages following cold exposure.We found that cold stress triggers increased macrophage activation accompanied by metabolic reprogramming of aerobic glycolysis.The discovery,by genome-wide RNA sequencing,of defective mitochondria in mice macrophages following cold exposure indicated that mitochondrial defects may contribute to this process.In addition,changes in metabolism drive the differentiation of macrophages by affecting histone modifications.Finally,we showed that histone acetylation and lactylation are modulators of macrophage differentiation following cold exposure.collectively,metabolism-related epigenetic modifications are essential for the differentiation of macrophages in cold-stressed mice,and the regulation of metabolism may be crucial for alleviating the harm induced by cold stress.

关键词： Low temperature Stress Autophagy Metabolic reprogramming Histone

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Cell type-specific proteomics uncovers a RAF15-SnRK2.6/OST1 kinase cascade in guard cells

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植物学报:英文版 2023年第9期65卷 2122-2137页

作者： Hongliang Wang Yubei Wang Tian Sang Zhen Lin Rongxia Li Weiwei Ren Xin Shen Bing Zhao Xiao Wang Xuebin Zhang Shaoqun Zhou Shaojun Dai Honghong Hu Chun-Peng Song Pengcheng Wang shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China shanghai bioprofile Technology company ltd Shanghai 200241China Center of Plant Germplasm Resources College of Life SciencesShanghai Normal UniversityShanghai 200234China National Key Laboratory of Crop Genetic Improvement College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Stress Adaptation and Improvement College of Life SciencesCollege of AgricultureAcademy for Advanced Interdisciplinary StudiesHenan UniversityKaifeng 475004China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China

Multicellular organisms such as plants contain various cell types with specialized functions.Analyzing the characteristics of each cell type reveals specific cell functions and enhances our understanding of organization and function at the organismal level.Guard cells(GCs)are specialized epidermal cells that regulate the movement of the stomata and gaseous exchange,and provide a model genetic system for analyzing cell fate,signaling,and function.Several proteomics analyses of GC are available,but these are limited in depth.Here we used enzymatic isolation and flow cytometry to enrich GC and mesophyll cell protoplasts and perform in-depth proteomics in these two major cell types in Arabidopsis leaves.We identified approximately 3,000 proteins not previously found in the GC proteome and more than600 proteins that may be specific to GC.The depth of our proteomics enabled us to uncover a guard cell-specific kinase cascade whereby Raf15and Snf1-related kinase2.6(SnRK2.6)/OST1(open stomata 1)mediate abscisic acid(ABA)-induced stomatal closure.RAF15 directly phosphorylated SnRK2.6/OST1 at the conserved Ser175 residue in its activation loop and was sufficient to reactivate the inactive form of SnRK2.6/OST1.ABAtriggered SnRK2.6/OST1 activation and stomatal closure was impaired in raf15 mutants.We also showed enrichment of enzymes and flavone metabolism in GC,and consistent,dramatic accumulation of flavone metabolites.Our study answers the long-standing question of how ABA activates SnRK2.6/OST1 in GCs and represents a resource potentially providing further insights into the molecular basis of GC and mesophyll cell development,metabolism,structure,and function.

关键词： abscisic acid guard cell metabolomics phosphorylation proteomics signaling

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