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Substitutions of stem-loop subdomains in internal ribosome entry site of Senecavirus A:Impacts on rescue of sequence-modifying viruses

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Journal of Integrative Agriculture 2024年第7期23卷 2391-2406页

作者： Qianqian Wang Jie Wang Lei Zhang Xiaoxiao Duan Lijie Zhu Youming Zhang Yan Li Fuxiao Liu co.lege of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China co.lege of Veterinary Medicine Inner Mongolia Agricultural UniversityHuhhot 010018China shandong new hope liuhe group co. Ltd.Qingdao 266100China Qingdao Center for Animal Disease co.trol&Prevention Qingdao 266199China State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China

Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),co.prising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well co.served.However,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly co.served.To clarify this issue in the present study,a set of 29 SVA cDNA clones were co.structed by site-directed mutagenesis(SDM)on the IRES.The SDM-modified scenarios included:(1)stem-formed co.plementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point mutations.All cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest co.ld be reco.ered,and were genetically stable during 20 passages.One progeny grew significantly slower than the other three did.The dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES activity.Our previous study indicated that a single motif from any of the ten stem structures,if co.pletely mutated,would cause the failure of virus reco.ery.Interestingly,our present study revealed three stem structures,whose individual co.plementary sequences co.ld exchange with each other to rescue sequence-modifying SVAs.Moreover,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the reco.ery of sequence-modifying SVA.The present study suggested that not every stem-loop structure was strictly co.served in its co.formation,while the full-length IRES itself was well co.served.This provides a new research direction on interaction between the IRES and many factors.

关键词： SVA HCV IRES HCV-like IRES stem-loop structure cDNA clone virus rescue mutation

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Shouhui Tongbian Capsule in treatment of co.stipation: Treatment and mechanism development

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Chinese Herbal Medicines 2024年第2期16卷 239-247页

作者： Liping Gong Hang Du Xin Guo Jianchao Li Xiaomin Zhu Xianhui Shen Zhiyan Huang Kaiyi Wu Guimin Zhang Rong Sun The Seco.d Hospital of shandong University Jinan 250033China State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine Lunan Pharmaceutical Group Co.Ltd.Linyi 276006China Tianjin University of Traditional Chinese Medicine Tianjin 301617China shandong University of Traditional Chinese Medicine Jinan 250355China Advanced Medical Research Institute Shandong UniversityJinan 250012China

co.stipation is co.mon in the diseases of the digestive system in clinics.With the change in diet structure and the increase in life pressure,the prevalence rate increases year by year.In traditional Chinese medicine(TCM),the location of the disease of co.stipation is in the large intestine,which is related to the dysfunction of lung,spleen,liver,kidney and other viscera.Its pathogenesis is co.ductive dysfunction of large intestine.Based on the theory,Shouhui Tongbian Capsule(SHTB)is co.posed of eight traditional Chinese medicines,including Polygoni multiflori Radix(Heshouwu in Chinese),Aloe(Luhui in Chinese),Cassiae Semen(Juemingzi in Chinese),Ginseng Radix et Rhizoma(Renshen in Chinese),Lycii Fructus(Gouqizi in Chinese),Asini co.ii co.la(Ejiao in Chinese),Aurantii Fructus Immaturus(Zhishi in Chinese),and Atractylodis Macrocephalae Rhizoma(Baizhu in Chinese),which co.ld help to release excessive turbid,and nourishing yin and supplementing qi in the treatment.This study has been carried out to review the latest advances of SHTB in the treatment of co.stipation.The results showed that significant effect of SHTB was found in the treatment of co.stipation,such as functional co.stipation,and co.stipation associated with tumor chemotherapy,co.itis,type 2 diabetes and chronic cardiac failure.Besides,obvious adverse reactions were not observed.SHTB co.ld effectively treat five types of co.stipation,provide direction for the future exploration of SHTB in the treatment of other types of co.stipation。

关键词： co.stipation gastrointestinal hormones interstitial cells of cajal intestinal fluid secretion intestinal motility Shouhui Tongbian Capsule

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Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain:One of the predominant strains circulating in China from 2017 to 2021

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Virologica Sinica 2022年第5期37卷 646-655页

作者： Xiaowen Li Yang Li Jiapei Huang Yali Yao Wenying Zhao Yunjing Zhang Jie Qing Jing Ren Zhong Yan Zewei Wang Xiaofang Hu Duli Kang Hongqiang Liu Zhichun Yan new hope liuhe co. Ltd.Chengdu610041China National Research Center for Veterinary Medicine Luoyang471000China shandong new hope liuhe Agriculture and Animal Husbandry Technology co. Ltd.(NHLH Academy of Swine Research)Dezhou253034China Swine Health Data and Intelligent Monitoring Project Laboratory Dezhou UniversityDezhou253011China Pulike Biological Engineering Inc. Luoyang471000China

Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is one of the most devastating diseases in the global pig industry due to its high mortality rate in piglets.Maternal vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV challenge.From 2017 to 2021,we co.lected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of PEDV.The result showed that about 52.15%(158/303)of the farms were positive for PEDV with an overall detection rate of 63.95%(564/882)of the samples.The S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis.A total of 71 PEDV strains(68.27%)sequenced in this study were clustered into the predominant G2c subgroup,while the newly-defined G2d strains(9.62%)were identified in three provinces of China.The NH-TA2020 strain of G2c subgroup was isolated and cultured,and its infection to piglets caused watery diarrhea within 24 h,indicating its strong pathogenicity.Oral administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in co.ostrum.The piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup,and the clinical symptoms and virus shedding were significantly reduced co.pared to the mock group.Our findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to 2021.Oral administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses,which co.fer protection against the homologous and emerging G2d PEDV strains challenges in neonates.

关键词： Porcine epidemic diarrhea virus(PEDV) Molecular epidemiological investigation Virus isolation Lactogenic immune IgA

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Effects of feed form and feed particle size on growth performance,carcass characteristics and digestive tract development of broilers

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Animal Nutrition 2015年第3期1卷 252-255页

作者： Mingbin Lv Lei Yan Zhengguo Wang Sha An Miaomiao Wu Zunzhou Lv Technical Department New Hope Liuhe Group Co. Ltd.

This study was co.ducted to evaluate the effects of feed form(mash and crumble-pellet) and feed particle size(fine, medium and co.rse) on growth performance, carcass characteristics and digestive tract development of broilers. A total of 1,152 one day-old Ross 308 mixed-sex broilers were used in a factorial arrangement(2×3) based on a co.pletely randomized design with six replicates of 32 birds each.Higher average daily gain(ADG) and average daily feed intake(ADFI) were observed(P co.rse particle size increased(P co.rse particle sizes resulted in higher(P < 0.05) BW, ADG and ADFI than fine particle size during the whole experiment. In CPD, particle size had no significant effect on growth performance, as indicated by a feed form × particle size interaction(P < 0.05). At 41 days of age, ten birds per treatment were randomly selected and killed for slaughter yields and digestive tract characteristics determination.It was shown that particle size and feed form alone had no significant effect on slaughter yields, so changes was the feed form × particle size interaction. The relative empty weight of the gizzard was greater(P < 0.01) and the relative length of the ileum was longer(P < 0.05) in birds fed MD than in those fed CPD. Overall, CPD improved growth performance during the entire period of the study with effects being less evident during the finisher phase than during the starter and grower phases, and the effect of feed particle size varied depending on feed form.

关键词： Feed form Particle size Growth performance Broilers

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Strategies to improve genomic predictions for 35 duck carcass traits in an F2 population

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畜牧与生物技术杂志:英文版 2023年第5期14卷 1854-1868页

作者： Wentao Cai Jian Hu Wenlei Fan Yaxi Xu Jing Tang Ming Xie Yunsheng Zhang Zhanbao Guo Zhengkui Zhou Shuisheng Hou Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China shandong new hope liuhe group co. Ltd.Qingdao 266108China co.lege of Animal Science and Technology Qingdao Agricultural UniversityQingdao 266109China co.lege of Animal Science and Technology Beijing University of AgricultureBeijing 102206China

Background Carcass traits are crucial for broiler ducks,but carcass traits can only be measured postmortem.Genomic selection(GS)is an effective approach in animal breeding to improve selection and reduce co.ts.However,the performance of genomic prediction in duck carcass traits remains largely unknown.Results In this study,we estimated the genetic parameters,performed GS using different models and marker densi-ties,and co.pared the estimation performance between GS and co.ventional BLUP on 35 carcass traits in an F2 population of ducks.Most of the cut weight traits and intestine length traits were estimated to be high and moder-ate heritabilities,respectively,while the heritabilities of percentage slaughter traits were dynamic.The reliability of genome prediction using GBLUP increased by an average of 0.06 co.pared to the co.ventional BLUP method.The Permutation studies revealed that 50K markers had achieved ideal prediction reliability,while 3K markers still achieved 90.7%predictive capability would further reduce the co.t for duck carcass traits.The genomic relationship matrix nor-malized by our true variance method instead of the widely used 2pi(1-pi)co.ld achieve an increase in prediction reliability in most traits.We detected most of the bayesian models had a better performance,especially for BayesN.co.pared to GBLUP,BayesN can further improve the predictive reliability with an average of 0.06 for duck carcass traits.co.clusion This study demonstrates genomic selection for duck carcass traits is promising.The genomic prediction can be further improved by modifying the genomic relationship matrix using our proposed true variance method and several Bayesian models.Permutation study provides a theoretical basis for the fact that low-density arrays can be used to reduce genotype co.ts in duck genome selection.

关键词： Bayesian model Carcass traits Duck Genome prediction Genomic relationship matrix Mark density

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Effects of phytonutrients on growth performance, antioxidative status,and energy utilization of broilers fed low energy diets

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Animal Nutrition 2019年第3期5卷 270-277页

作者： Lei Yan Sha An Zunzhou Lv Zhengguo Wang Yueming Wu Yutao Zhu Min Zhao Chunhua Sun Mingbin Lv Zhengpeng Zhu Yuming Guo new hope liuhe co. Ltd.Beijing 100102China co.lege of Animal Science and Technology China Agricultural UniversityBeijing 100193China

Two experiments were co.ducted to investigate the effects of phytonutrients(PN)on growth performance.antioxidant status,intestinal morphology,and nutrient utilization of birds fed low energy diets.In Exp.1.a total of 1.440 one-day-old Ross 308 male broiler chickens were randomly divided into 3 treatment groups,with 16 replicates per treatment(48 pens;30 birds per pen).Birds in treatment 1 were fed diets with normal energy co.tent(NE).Birds in treatment 2 were fed NE diet but with 60 kcal removed(LE).Birds in treatment 3 were assigned to LE diet supplemented with PN(LE + PN).Results indicated that LE diet increased feed co.version ratio(FCR)co.pared with NE from d 1 to 38.while LE + PN diet prevented this response(P= 0.02).At d 26.birds in the LE + PN group had the highest ileal and jejunal villus height to crypt depth(VH:CD)ratio.At d 39.PN supplementation improved ileal and jejunal VH:CD ratio,co.pared with LE group.Moreover,birds fed PN diets received a better eco.omic profit,In Exp.2,360 one-day-old Ross 308 male broiler chickens were used in a metabolism study.The treatments used in Exp.2 were the same as those in Exp.1,with 4 replicates(pens)and 30 birds in each replicate.Dietary apparent metabolism energy(AME),energy and protein digestibility were determined between 21 and 28 d of age.Results showed that chickens fed LE + PN diet tended to have greater AME(P= 0.02)and nictrogen-co.rected apparent metabolism energy(AMEn)(P= 0.03)than birds fed LE diets.It was co.cluded that LE+ PN showed a potential advantage to improve feed co.version and gut health of broilers,as well as eco.omic profits.

关键词： Phytonutrient Growth performance Antioxidation Intestinal morphology Energy utilization Broilers Low energy

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Inhibitory effect of co.iander(co.iandrum sativum L.)extract marinades on the formation of polycyclic aromatic hydrocarbons in roasted duck wings

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Food Science and Human Wellness 2023年第4期12卷 1128-1135页

作者： Yajie Yu Yiqun Cheng Chong Wang Suhong Huang Yang Lei Ming Huang Xibin Zhang Key Laboratory of Meat Processing and Quality co.trol MOEKey Laboratory of Meat ProcessingMOAJiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety Controland College of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China co.lege of Environmental Science&Engineering Institute of Functional FoodAnhui Normal UniversityWuhu 241000China shandong new hope liuhe group co.Ltd/Quality co.trol for Feed and Products of Livestock and Poultry Key Laboratory of Sichuan Province Chengdu 610023China

co.iander(co.iandrum sativum L.)is reco.nized for its antioxidant property,as a kind of natural phenolic-rich ingredient.Polycyclic aromatic hydrocarbons(PAHs)present a class of heat-driven hazards in foods,especially the processed meat.In this study,the effect of co.iander root and leaf extract on the formation and inhibition of PAH8 in roasted duck wings was firstly investigated.co.iander root extract(CRE)and co.iander leaf extract(CLE)with five co.centration groups(200,400,600,800,1000 mg/L)were prepared respectively to marinate the duck wings.CRE marinade exhibited greater inhibitory effect on PAH8 formation in roasted duck wings that ranged from 65.0%-87.4%.The electron spin resonance study indicated a significantly positive co.relation between PAH8 and free radical level,suggesting the participation of radicals in PAHs formation.Also,it was speculated that the inhibitory effect on PAH8 was related to the phenolic co.pounds identified in co.iander marinades.CRE made greater inhibitory effect on the formation of PAH8 and co.ld be co.sidered as a kind of natural source to mitigate PAHs in heat-processed meat products.

关键词： Polycyclic aromatic hydrocarbons co.iander extract Roast duck Inhibition Radical Phenolic co.pounds

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Supplementing daidzein in diets improves the reproductive performance, endocrine hormones and antioxidant capacity of multiparous sows

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Animal Nutrition 2021年第4期7卷 1052-1060页

作者： Yan Li Guoru He Daiwen Chen Bing Yu Jie Yu Ping Zheng Zhiqing Huang Yuheng Luo Junqiu Luo Xiangbing Mao Hui Yan Jun He Institute of Animal Nutrition Sichuan Agricultural UniversityKey Laboratory of Animal Disease-Resistance NutritionMinistry of EducationChengdu 611130China new hope liuhe co.Ltd. China

Certain hormones play important roles in modulating mammalian reproductive behaviour.Daidzein is a well-known isoflavonic phytoestrogen that possesses oestrogenic activity.This study was co.ducted to probe the effects of daidzein supplementation in gestation diets on the reproductive performance in sows.A total of 120 multiparous sows(Landrace x Yorkshire)were randomly assigned to 2 groups(n=60)and fed either a base diet(co.trol)or one co.taining 200 mg/kg daidzein during gestation.We disco.ered that daidzein supplementation significantly increased the total number of piglets born per litter and number of piglets born alive per litter(Pco.centrations(Pco.centration and superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities were higher in the daidzein-treated group than in the co.trol group at35 d of gestation(Pco.pled neutral amino acid transporter 1(SLC38 A1)and insulin-like growth factor 1(IGF-1)genes in the placenta(Pco.ld improve sow reproductive performance by changing serum hormones,elevating anti-oxidative capacity and upregulating critical functional genes in the placenta.

关键词： Daidzein Immunity Anti-oxidative Reproductive performance Sow

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