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Breeding and molecular cytogenetic identification of wheat-Thinopyrum intermedium addition lines resistant to powdery mildew

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Chinese Science Bulletin 2002年第22期47卷 1892-1896,1939页

作者： Shubing Liu Honggang Wang shandong agr univ Agron Coll Tai An 271018 Peoples R China

Wheat-related species Th. intermedium was used to cross with common wheat Yannong 15. In the self progenies of the hybrid, two addition lines, II -1-7-1 and II -3-3-2, stable in cytology, were developed by cytology and powdery mildew resistance identification. Their chromosome number were 2n = 44 and formed 22 bivalents at PMC MI. In F1 of the two addition lines crossing with Yannong 15, there appeared about one univalent at PMC MI, respectively. Resistance identification in greenhouse and field using the No. 15 and mixed strains of E. gramnis f. sp. tritici showed that they were immune to powdery mildew. Chromosome number and resistance identification using the F2 single plants of the addition line crossing with Yannong 15 indicated that the resistant gene was located on the alien chromosomes. In situ hybridization using St and E genomic DNA as probe showed that the added chromosome in the two addition lines probably came from the E genome of Th. intermedium, which indicated that a pair of E genome

关键词： wheat powdery mildew addition line Th. intermedium in situ hybridization.

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Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and in vivo

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Chinese Science Bulletin 2002年第23期47卷 1999-2005页

作者： Kewei Zhang Jianmei Wang Guodong Yang Xingqi Guo Fujiang Wen Decai Cui Chengchao Zheng shandong agr univ Coll Life Sci Tai An 271018 Peoples R China

Inclusion of MARs in transgene cassettes enhances their expression and reduces position-effect variations in the transgenic host. Four new MARs (TM2, TM3, AM1 and AM2) were isolated from tobacco and Arabidopsis by PCR method. The nuclei isolated from suspension-cultured cells of rice were used to prepare nuclear matrix. With a characterized MAR (TM1) as a positive control, the Matrix-MAR interactions were tested by an in vitro binding assay to identify the DNA sequences as MARs and their binding strength to nuclear matrix in vitro was compared. The results showed that TM2 and TM3 had stronger binding strength than TM1. To determine the functions of the four new MARs in vivo, binary vectors pBI121 carrying a uidA GUS reporter gene were modified with direct repeat MARs inserted on both sides of the reporter gene cassette and were transferred into tobaccos via agrobacterium-mediated transformation procedure. Quantitative GUS assays of the transgenic tobaccos showed that when flanking a GUS reporter gene

关键词： MAR isolation nuclear matrix in vitro binding assay β-glucuronidase foreign gene expression high efficient expression vector construction.

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Plant Circular RNAs （circRNAs）： Transcriptional Regulation Beyond miRNAs in Plants

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Molecular Plant 2016年第2期9卷 192-194页

作者： Sablok, Gaurav Zhao, Hongwei Sun, Xiaoyong univ Technol Sydney Plant Funct Biol & Climate Change Cluster C3 POB 123Broadway Sydney NSW 2007 Australia Nanjing agr univ Dept Plant Pathol Weigang Rd Nanjing 210095 Jiangsu Peoples R China shandong agr univ Coll Informat Sci & Engn Agr Big Data Res Ctr Tai An 271018 Shandong Peoples R China

Current plant functional genomics is converging on two as- pects to provide sustainable solutions to cater to the demands of the growing population： 1） engineering crops for sustainable food security, where recently identified CRISPR/Cas is playing a detrimental role （Belhaj et al., 2014） and 2） identifying regulators of the post-transcriptional regulation, which can be functionally engineered. Canonical splicing has been widely seen and associated with functional protein diversity in plants （Min et al., 2015）（Figure 1）. Concurrent patterns of exonic and intronic splicing have revealed several new isoforms,

关键词： detrimental engineered functional

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Salt glands in Glycine soja ***

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Chinese Science Bulletin 1999年第10期44卷 923-926页

作者： Jingmei Lu Youliang Liu Bo Hu Bingchang Zhuang Nanjing agr univ Nanjing 200095 Peoples R China NE Normal univ Sch Life Sci Changchun 130024 Peoples R China

A scanning electron microscope observation is carried out on Glycine soja L., which grows around the estuary of the Yellow River, in Kenli County, shandong Province. It is first discovered that there are salt glands, in the shape of balls, on the surface of the stems and leaves of G. soja L., and distributed at the intercellular layer. With a handle at the base, the salt glands are not equal in size, and show a layer-upon-layer structure. They have a diameter of 21 .6 μm, a handle length of 1.2 μm, their secretory pore’s diameter is 5.6 μm. There are two ways to secrete salt ions: younger glands secrete salt ions through the secretory pore, and mature glands by breaking themselves.

关键词： Glycine soja L. salt glands secretory structure.

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Molecular Mechanisms and Therapy Strategies of agr2 induced Chemoresistance

Molecular Mechanisms and Therapy Strategies of AGR2 induced ...

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中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Mengqi Jia Yunqiu Wang Huanmin Niu Huiqing Yuan Institute of medical science The second hospital of Shandong University Key Laboratory of Experimental Teratology of the Ministry of Education Shandong University School of Basic Medicine

Malignant tumors are serious diseases that seriously threaten human health. The treatment is mainly based on surgical treatment, radiation therapy, chemotherapy and immunotherapy in recent years. The low selectivity of drug resistance and precision medication is a difficult problem to control. Anterior gradient 2(agr2), an endoplasmic reticulum(ER)-resident protein-disulfide isomerase(PDI), is associated to cancer development and malignant progression. There are few studies on agr2 and tumor resistance. Screening agr2 expression and sensitivity to cytotoxic chemotherapy drugs and targeted drugs of tumor cells, we show that low level of agr2 promotes the Multi-drug resistant phenotype of prostate cancer and lung cancer. We found that both intracellular and secreted agr2 participated in drug resistance through different mechanisms. Firstly, extracellular agr2 directly interacts with VEGFA to promote angiogenesis and resistance to bevacizumab in prostate cancer. Importantly, GSH and cabozantinib effectively blocked the pro-angiogenic effect of rhagr2 in vitro and in vivo, providing evidence that secreted agr2 acts as a predictive biomarker for selection of angiogenesis-targeting therapeutic drugs based on its levels in the circular system. We also provided evidence identifying intracellular agr2 negatively regulates MRP2 expression and mediates multidrug resistance of tumor chemotherapy. Molecular mechanism indicates agr2 promote MRP2 endocytosis and degradation through promote its ubiquitination. In addition, agr2 negatively regulates the proteasome activity of tumor cells through regulates the proteasome RP-CP association, and mediates tumor cell sensitive to proteasome inhibitor Bortezomib. Finally, we provide a Docetaxel and Bortezomib sequential therapy strategy to overcomes acquired resistance in Chemotherapy. Those founds highlighting the importance of agr2 as a predictive marker for selection of subgroup patients in chemotherapy.

关键词： agr2 disulfide isomerase multidrug resistance angiogenesis proteasome activity

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Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China

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World Journal of Pediatrics 2020年第3期16卷 284-292页

作者： Chen Sun Qing Wang Wen-Ting Li De-Nian Wen Chang-Hui Chen Xin Yang Wei Shi Qing-Hong Meng Kai-Hu Yao Su-Yun Qian Pediatric Intensive Care Unit Beijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthNanLiShi Road 56Xicheng DistrictBeijing 100045China Key Laboratory of Major Diseases in Children National Key Discipline of Pediatrics(Capital Medical University)National Clinical Research Center for Respiratory DiseasesBeijing Key Laboratory of Pediatric Respiratory Infection DiseasesBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Respiratory Department Qilu Children's HospitalShandong UniversityJinan 250022China Department of Pediatrics People's Hospital of Zhongjiang CountyNo.96 Da-bei StreetDeyang 618100China Youyang Hospital The First Affiliated Hospital of Chongqing Medical UniversityPeople's Hospital of Chongqing Youyang CountyChongqing 409899China

Background The molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus (***) in general pediatric wards and county-level hospitals were rarely reported in *** Staphylococcus aureus was isolated from children hospitalized with respiratory tract infection (RTI) in Zhongjiang and Youyang counties in *** isolates were typed by multilocus sequence,staphylococcal protein A,accessory gene regulator (agr),and staphylococcal cassette chromosome mec [SCCmec,for methicillin-resistant *** (MRSA) only].Polymerase chain reaction was used to screen 21 super-antigen (SAg) genes and panton-valentine leukocidin (pvl).Antimicrobial susceptibility testing was performed by E *** A total of 2136 children were ***,125 (5.9%) children carried ***,among which MRSA accounted for 42.4%.ST59-SCCmec type Ⅳ-t437-agr group Ⅰ (58.5%) was the most prevalent genotype in MRSA,and ST188-t189-agr group Ⅰ (22.2%) was the top genotype in methicillin-sensitive *** (MSSA).The pvl carriage rate in MRSA and MSSA was 15.1% and 9.7%,respectively (P =0.4112).About 96.8% of *** isolates were positive for at least one SAg *** most common SAg gene profile in the dominant ST59 clone was seb-sek-seq (42.8%).All *** isolates were resistant to penicillin and erythromycin (minimum inhibitory concentration 90 was > 32 and 256 mg/L to penicillin and erythromycin,respectively),but usually susceptible to other tested non-β-lactam *** Staphylococcus aureus and MRSA were detected with a high frequency in children with RTI in county-level hospitals of ***59-SCCmec type Ⅳ-t437-agr group Ⅰ was the dominant MRSA *** *** isolates exhibited high resistance to penicillin and erythromycin.

关键词： Antimicrobial susceptibility Chinese children Molecular characteristics Staphylococcus aureus

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Fungal diversity notes 1512–1610: taxonomic and phylogenetic contributions on genera and species of fungal taxa

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Fungal Diversity 2022年第6期117卷 1-272页

作者： Ruvishika S.Jayawardena Kevin D.Hyde Song Wang Ya‑Ru Sun Nakarin Suwannarach Phongeun Sysouphanthong Mohamed A.Abdel‑Wahab Faten A.Abdel‑Aziz Pranami D.Abeywickrama Vanessa P.Abreu Alireza Armand AndréAptroot Dan‑Feng Bao Dominik Begerow Jean‑Michel Bellanger Jadson D.P.Bezerra Digvijayini Bundhun Mark S.Calabon Ting Cao Taimy Cantillo João LVRCarvalho Napalai Chaiwan Che‑Chih Chen Régis Courtecuisse Bao‑Kai Cui Ulrike Damm Cvetomir M.Denchev Teodor T.Denchev Chun Y.Deng Bandarupalli Devadatha Nimali Ide Silva Lidiane Ados Santos Nawal K.Dubey Sylvain Dumez Himashi SFerdinandez André L.Firmino Yusufon Gaforov Achala J.Gajanayake Deecksha Gomdola Sugantha Gunaseelan Shucheng‑He Zin H.Htet Malarvizhi Kaliyaperumal Martin Kemler Kezhocuyi Kezo Nuwan DKularathnage Marco Leonardi Ji‑Peng Li Chunfang Liao Shun Liu Michael Loizides Thatsanee Luangharn Jian Ma Hugo Madrid S.Mahadevakumar Sajeewa S.N.Maharachchikumbura Dimuthu S.Manamgoda María P.Martín Niranjan Mekala Pierre‑Arthur Moreau Yan‑Hong Mu Pasouvang Pahoua Dhandevi Pem Olinto L.Pereira Wiphawanee Phonrob Chayanard Phukhamsakda Mubashar Raza Guang‑Cong Ren Andrea C.Rinaldi Walter Rossi Binu C.Samarakoon Milan CSamarakoon Vemuri V.Sarma Indunil C.Senanayake Archana Singh Maria F.Souza Cristina M.Souza‑Motta Adriano A.Spielmann Wenxin Su Xia Tang XingGuo Tian Kasun M.Thambugala Naritsada Thongklang Danushka S.Tennakoon Nopparat Wannathes DingPeng Wei Stéphane Welti Subodini N.Wijesinghe Hongde Yang Yunhui Yang Hai‑Sheng Yuan Huang Zhang Jingyi Zhang Abhaya Balasuriya Chitrabhanu SBhunjun Timur S.Bulgakov Lei Cai Erio Camporesi Putarak Chomnunti Y.S.Deepika Mingkwan Doilom Wei‑Jun Duan Shi‑Ling Han Naruemon Huanraluek EBGareth Jones NLakshmidevi Yu Li Saisamorn Lumyong Zong‑Long Luo Surapong Khuna Jaturong Kumla Ishara S.Manawasinghe Ausana Mapook Wilawan Punyaboon Saowaluck Tibpromma Yong‑Zhong Lu JiYe Yan Yong Wang Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Innovative Institute for Plant Health Zhongkai University of Agriculture and EngineeringGuangzhou 510225P.R.China Department of Plant Pathology College of AgricultureGuizhou UniversityGuiyang 550025GuizhouChina Research Center of Microbial Diversity and Sustainable Utilization Chiang Mai UniversityChiang Mai 50200Thailand Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Biotechnology and Ecology Institute Ministry of Agriculture and ForestryP.O.Box:811Vientiane CapitalLao PDR Department of Botany and Microbiology Faculty of ScienceSohag UniversitySohag 82524Egypt Beijing Key Laboratory of Environment-Friendly Management on Fruit Diseases and Pests in North China Institute of Plant ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Departamento de Fitopatologia Universidade Federal de ViçosaViçosaMinas GeraisBrazil Laboratório de Botânica/Liquenologia Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulAvenida Costa e Silva S/NBairro UniversitárioCampo GrandeMato Grosso do Sul CEP 79070-900Brazil College of agriculture and Biological Sciences Dali UniversityDali 671003YunnanChina Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Institute of Plant Science and Microbiology Universität HamburgOrganismic Botany and MycologyOhnhorststr.1822609 HamburgGermany CEFE CNRSUniv.MontpellierEPHEIRDINSERM1919Route de Mende34293 Montpellier Cedex 5France Setor de Micologia Departamento de Biociências e TecnologiaInstituto de Patologia Tropical e Saúde PúblicaUniversidade Federal de GoiásRua 235S/NSetor UniversitárioGoiâniaGO CEP:74605-050Brazil Division of Biological Sciences College of Arts and SciencesUniversity of the Philippines Visayas5023 MiagaoIloiloPhilippines CA

This article is the 14th in the Fungal Diversity Notes series,wherein we report 98 taxa distributed in two phyla,seven classes,26 orders and 50 families which are described and *** in this study were collected from Australia,Brazil,Burkina Faso,Chile,China,Cyprus,Egypt,France,French Guiana,India,Indonesia,Italy,Laos,Mexico,Russia,Sri Lanka,Thailand,and *** are 59 new taxa,39 new hosts and new geographical distributions with one new *** 59 new species comprise Angustimassarina kunmingense,Asterina lopi,Asterina brigadeirensis,Bartalinia bidenticola,Bartalinia caryotae,Buellia pruinocalcarea,Coltricia insularis,Colletotrichum fexuosum,Colletotrichum thasutense,Coniochaeta caraganae,Coniothyrium yuccicola,Dematipyriforma aquatic,Dematipyriforma globispora,Dematipyriforma nilotica,Distoseptispora bambusicola,Fulvifomes jawadhuvensis,Fulvifomes malaiyanurensis,Fulvifomes thiruvannamalaiensis,Fusarium purpurea,Gerronema atrovirens,Gerronema favum,Gerronema keralense,Gerronema kuruvense,Grammothele taiwanensis,Hongkongmyces changchunensis,Hypoxylon inaequale,Kirschsteiniothelia acutisporum,Kirschsteiniothelia crustaceum,Kirschsteiniothelia extensum,Kirschsteiniothelia septemseptatum,Kirschsteiniothelia spatiosum,Lecanora immersocalcarea,Lepiota subthailandica,Lindgomyces guizhouensis,Marthe asmius pallidoaurantiacus,Marasmius tangerinus,Neovaginatispora mangiferae,Pararamichloridium aquisubtropicum,Pestalotiopsis piraubensis,Phacidium chinaum,Phaeoisaria goiasensis,Phaeoseptum thailandicum,Pleurothecium aquisubtropicum,Pseudocercospora vernoniae,Pyrenophora verruculosa,Rhachomyces cruralis,Rhachomyces hyperommae,Rhachomyces magrinii,Rhachomyces platyprosophi,Rhizomarasmius cunninghamietorum,Skeletocutis cangshanensis,Skeletocutis subchrysella,Sporisorium anadelphiae-leptocomae,Tetraploa dashaoensis,Tomentella exiguelata,Tomentella fuscoaraneosa,Tricholomopsis lechatii,Vaginatispora favispora and Wetmoreana *** new combin

关键词： 59 New taxa 39 New records Ascomycota Basidiomycota Dothideomycetes Lecanoromycetes Leotiomycetes Sordariomycetes Ustilaginomycotina

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