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decommissioning of Mature Oil Fields and Artisanal Fisheries： The Case of Todosos santos Bay, Brazil

Journal of Environmental Science and Engineering(A) 2012年第1期1卷 73-81页

作者： Henrique de Barros Marise Paranagua Viviane Almeida Mauro MeloJunior Patricia Ribeiro DLCH-Federal Rural University of Pernambuco Recife 52171-900Brazil Federal Rural University of Pernambuco Recife 52171-900Brazil secretaria de MeioAmbiente Prefeitura do Recife 50030-230Brazil UAST-Federal Rural University of Pernambuco Recife 52171-900Brazil

Consequences of decommissioning oil fields on artisanal fishing activities are still little known in the literature. This paper is intended to shed some light on a process of dismantling and sinking of oil and gas structures in shallow waters, with severe disturbing impacts on low income artisanal fishing activities. From a socio-economic perspective, the relationship of oil industry with local communities is described, with the main perceived problems pointed out in local fishermen leadership perspective. The notions of ＂damages＂ and ＂mitigation＂ used by the oil industry are discussed in connection to the expansion and dismantling of oil installations during the past 20 yrs. A comparative view of oil fields decommissioning in Europe and Brazil during the late 1990s suggests the need to review transparency and social commitment standards which have been far less prominent in this Brazilian case. The authors believe that the Brazilian oil industry has acquired a social and environmental debt towards the whole society, as far as it has been unable to establish a clear and effective process for decommissioning their oil installations within the artisanal fishing areas of the Todosos santos Bay. Furthermore, the discussion of fair and specific compensations has been avoided, which otherwise would be instrumental to regain local economic conditions found among fishermen just few decades ago.

关键词： decommissioning oil fields mature oil fields environmental damages fishing communities Todosos santos Bay.

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Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori(Lepidoptera:Bombycidae)

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Open Journal of Animal Sciences 2013年第4期3卷 343-353页

作者： Roxelle Ethienne Ferreira Munhoz Thais Souto Bignotto Naiara Climas Pereira Claudia Regina das Neves saez Rafaela Bespalhuk Veronica Aureliana Fassina Graziele Milani Pessini Mayarha Patricia dequigiovanni Baggio Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Shunsuke Mizuno Willian Shigeaki Aita Maria Aparecida Fernandez departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Universidade Estadual do Oeste do Parana CascavelBrasil Fiacao de Seda Bratac S.A.Bastos Sao PauloBrasil

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced?thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

关键词： Silkworm Pesticide Drift Mortality Symptomatology Chlorantraniliprole

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Oficina das Letras： Practical Approach to Writing in a Psychiatric Hospital

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Psychology Research 2018年第8期8卷 367-374页

作者： Barbara de Caldas Melo Leila Akemi Evangelista Kusano Rosiberton Pereira da Cruz Michelle Andreza Falcao Rodrigues Centro Universitario UDF Brasilia (DF) Brasil secretaria de Estado de saude do Distrito Federal Brasilia (DF) Brasil

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全球化及大豆种植向阿根廷的半干旱生态系的扩展

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AMBIO－人类环境杂志 2005年第3期34卷 261-262页

作者： H. Ricardo Grau T. Mitchell Aide N. Ignacio Gasparri 许天虎（译）林宝法（校） department of Biology Universidad de Puerto Rico PO Box 23360 Rio Piedras Puerto Rico Puerto Rico 00931 USA department of Biology Universidad de Puerto Rico PO Box 23360 Rio Piedras Puerto Rico Unidad de Manejo del Sistema de Evacuacion Forestal secretaria de Ambiente y desarrollo Sustentable san Martin 451 C1004AA1 Ciudad de Buenos Aires Argentina 不详

砍伐森林是各自然、社会系统的地方特殊属性与通常在较大空间范围内发挥作用的驱动力量之间发生复杂相互作用的产物[1].社会与经济的全球化,特别是通讯与运输障碍的减少,已提升了全球环境变化(如砍伐森林)驱动因素的相对重要性.例如,农... 详细信息

砍伐森林是各自然、社会系统的地方特殊属性与通常在较大空间范围内发挥作用的驱动力量之间发生复杂相互作用的产物[1].社会与经济的全球化,特别是通讯与运输障碍的减少,已提升了全球环境变化(如砍伐森林)驱动因素的相对重要性.例如,农业的扩展正越来越强烈地受到在世界范围内迅速传播的农业技术改革的影响响[2],也因一些国家的巨大人口数量(如中国)或较高的生活水平(如美国、欧盟)而受到这些对全球市场造成不均衡影响的国家的消费方式变化的影响.

关键词：全球化大豆种植扩展生态系半干旱阿根廷全球环境变化社会系统驱动因素

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Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

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World Journal of Gastroenterology 2009年第12期15卷 1465-1471页

作者： Hivana Patricia Melo Barbosa Luisa Caricio Martins Sidney Emanuel Batista dos santos samia demachki Mnica Baraúna Assumpo Charliana Damasceno Arago Tereza Cristina de Oliveira Corvelo Laboratory of Immunogenetics Federal University of Pará66075-110BelémBrazil Nucleus of Tropical Medicine Federal University of Pará66055-240BelémBrazil Laboratory of Human and Medical Genetics Federal University of Pará66075-110BelémBrazil department of Pathology Joo de Barros Barreto Universitary HospitalFederal University of Pará66073-000BelémBrazil department of Endoscopy Joo de Barros Barreto Universitary HospitalFederal University of Pará66073-000BelémBrazil

AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS： Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method （PCRRFLP） and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS： A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL- 1RN＊2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL- RN＊2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastricCONCLUSION： Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.

关键词： Helicobacter pylori Interleukin 1β gene Interleukin-1 receptor antagonist gene TNF-α gene Cag pathogenicity island

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Low Genetic Polymorphism at the Cytochrome C Oxidase I in Silkworm Strains of the Brazilian Germplasm Bank

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Open Journal of Genetics 2014年第3期4卷 202-209页

作者： Veronica Aureliana Fassina Thais Souto Bignotto Roxelle Ethienne Ferreira Munhoz Bruno Fulan Juliana Pereira Bravo Laura B.Garay Rafaela Bespalhuk Claudia Regina das Neves saez Naiara Climas Pereira Graziele Milani Pessini Maria Aparecida Fernandez departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaUEMParanaBrasil Seda y Fibras S.R.L. HernandariasParaguay

Nucleotide sequences have been used to distinguish species and specimens for many years. More recently, the use of a partial sequence of 650 bp of the cytochrome c oxidase I, COI mitochondrial gene, has been proposed for species identification, known as DNA barcodes. In this work, a short sequence of the DNA barcode is described—approximately 250 bp, named as “DNA mini-barcode”—to molecularly identify different silkworm strains maintained at the unique public Germplasm Bank of Bombyx mori, at the Universidade Estadual de Maringá, UEM, Brazil. Analysis revealed no significant differences among the silkworm strains. The phylogenetic tree obtained by the neighbor-joining method and K2P distance, in which specimens of B. mandarina were used as outgroup, clustered all the specimens of B. mori in a unique clade. Genetic variability detect within B. mori was low or nonexistent. In conclusion, the partial region of 250 bp of the mitochondrial gene COI herein analyzed may not be efficient to discriminate silkworm strains from the UEM Germplasm Bank of Bombyx mori.

关键词： Bombyx mori Mitochondrial Gene COI DNA Mini-Barcode

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New approach to assess sperm DNA fragmentation dynamics： Fine-tuning mathematical models

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Journal of Animal Science and Biotechnology 2017年第3期8卷 592-600页

作者： Isabel Ortiz Jesus Dorado Jane Morrell Jaime Gosalvez Francisco Crespo Juan M.Jimenez Manuel Hidalgo Veterinary Reproduction Group Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba 14071 Cordoba Spain department of Clinical Sciences Division of Reproduction Swedish University of Agricultural Sciences Box 7054 SE-75007 Uppsala Sweden department of Biology Universidad Aut6noma de Madrid 28049 Madrid Spain department of Reproduction Centro Militar de Cria Caballar (FESCCR-Ministry of Defense) 05005 Avila Spain department of Chemistry and Physics of Materials University of Salzburg Hellbrunner StraBe 34/111 A-5020 Salzburg Austria

Background： Sperm DNA fragmentation（sDF） has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if s DF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model（linear regression, exponential or polynomial） for DNA fragmentation over time in frozen-thawed donkey ***： After submitting post-thaw semen samples to no centrifugation（UDC）, sperm washing（SW） or single layer centrifugation（SLC） protocols, sD F values after 6 h of incubation were significantly lower in SLC samples than in SW or *** of determination（R-2） values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation（aSDF） were obtained for SW, fol owed by SLC and ***： SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. Moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule.

关键词： Colloid centrifugation Dynamics Fine-tuning Mathematical models Sperm DNA fragmentation

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detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori

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Open Journal of Genetics 2014年第5期4卷 370-377页

作者： Claudia Regina das Neves saez Roxelle Ethienne Ferreira Munhoz Naiara Climas Pereira Thais Souto Bignotto Veronica Aureliana Fassina Graziele Milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Maria Aparecida Fernandez departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

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Schistosomiasis Mansoni in the Amazon Region:Malacological Surveys of Intermediate Hosts for the Identification of Disease Transmission Areas in Belem,Para,Brazil

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Journal of Pharmacy and Pharmacology 2019年第2期7卷 51-60页

作者： Christiane de Oliveira Goveia Ricardo Jose de Paula Souza e Guimaraes Marcio Roberto Teixeira Nunes Isabelle Helena Lima Dias Martin Johannes Enk Instituto Evandro Chagas Secretaria de Vigilancia em SaudeMinisterio da Saude-IEC/SVS/MS-Secao de ParasitologiaLaboratario de Parasitoses IntestinaisEsquistossomose e MalacologiaAnanindeuaParaBrasil Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia Universidade do Estado do Para-UEPABelemParaBrasil Instituto Evandro Chagas Secretaria de Vigilancia em SaudeMinisterio da SaudeLaboratorio de Geoprocessamento-LABGEO/IEC/SVS/MSAnanindeuaParaBrasil Instituto Evandro Chagas Secretaria de Vigilancia em SaudeMinisterio da SaudeCentro de Inovacoes Tecnologicas-CIT/IEC/SVS/MSAnanindeuaParaBrasil

In Brazil,schistosomiasis occurrence is related to the presence of the etiological agent Schistosoma *** presence of mollusks for Biomphalaria genus is a necessary condition for development of the parasite *** Parástate,the species ***,***,*** and *** have already been identified,with reports of the last two occurring in Belé*** aim of this study was to determine the spatial distribution of Biomphalaria species in Belém,Pará,Brazil,identifying risk areas for schistosomiasis *** malacological surveys were carried out between September 2013 and October 2017 in 35 neighborhoods of Belé*** collection points were georeferenced using a Global Positioning System(GPS)receiver and the Kernel density Estimation(Kde)was used to the spatial *** mollusks,after measurement,were exposed to light to verify the presence of *** ***,mollusks were sacrificed and fixed for dissection and morphological identification of *** surveys resulted in a total of 10,803 mollusks collected,of which 9,367(86.70%)specimens were *** the analyzed mollusks,5,820(62.14%)were identified as *** and 3,547(37.86%)as *** presence of the parasite was observed in 208(2.22%)specimens,all *** mollusks were found in six neighborhoods:Guamá(51 mollusks/24.5%),sacramenta(47 mollusks/22.6%),Telégrafo(47 mollusks/22.6%),Terra Firme(36 mollusks/17.3%),Condor(20 mollusks/9.6%)and Barreiro(7 mollusks/3.4%).This study confirms that *** species actively participates as intermediate host in the disease maintenance in Belé*** Kde allowed to analyze the spatial distribution of collection sites and consequently to determine possible risk areas of schistosomiasis transmission in *** development of maps identifying sites with schistosomiasis positive snails may support efforts of this municipality by directing activities related to endemic disease control.

关键词： Biomphalaria spp. Schistosoma mansoni intermediate hosts schistosomiasis spatial analysis

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C-reactive protein is differentially modulated by co-existing infections,vitamin deficiencies and maternal factors in pregnant and lactating indigenous Panamanian women

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Infectious Diseases of Poverty 2017年第1期6卷 819-832页

作者： Doris Gonzalez-Fernandez Emerita del Carmen Pons delfina Rueda Odalis Teresa Sinisterra Enrique Murillo Marilyn E.Scott Kristine G.Koski School of Dietetics and Human Nutrition Macdonald Campus of McGill University21111 Lakeshore RoadSte-Anne-de-BellevueQC H9X 3V9Canada department of Nutritional Health Ministry of HealthAnconPanama CityPanama “Comarca Ngabe-Bugle”Health Region Ministry of HealthSan FelixChiriqui ProvincePanama “Panama Norte”Health Region Ministry of HealthLas Cumbres SquareTransithmian HighwayPanama CityPanama department of Biochemistry University of PanamaSimon Bolivar Avenue(Transithmian Highway)Panama CityPanama department of Biochemistry University of PanamaManuel Espinoza Batista and Jose De Fabrega AvenuesPanama CityPanama Institute of Parasitology and Centre for Host-Parasite Interactions Macdonald Campus of McGill University21111 Lakeshore RoadSte-Anne-de-BellevueQC H9X 3 V9Canada School of Dietetics and Human Nutrition and Centre for Host-Parasite Interactions Macdonald CampusMcGill University21111 Lakeshore RoadSte-Anne-de-BellevueQC H9X 3 V9Canada

Background:The usefulness of C-reactive protein(CRP)as a non-specific marker of inflammation during pregnancy and lactation is unclear in impoverished populations where co-existing infections and vitamin deficiencies are ***:This cross-sectional study in Panama recruited 120 pregnant and 99 lactating Ngäbe-Buglewomen from 14 communities in rural *** history,indoor wood smoke exposure,fieldwork,BMI,vitamins A,B_(12),D,and folic acid,and inflammation markers(CRP,neutrophil/lymphocyte ratio(NLR),plateletcrit and cytokines)were *** regressions explored both associations of CRP with other inflammatory markers and associations of CRP and elevated CRP based on trimester-specific cut-offs with maternal factors,infections and vitamin ***:CRP was higher in pregnancy(51.4±4.7 nmol/L)than lactation(27.8±3.5 nmol/L)and was elevated above trimester specific cut-offs in 21%of pregnant and 30%of lactating *** deficiencies were common(vitamin A 29.6%;vitamin D 68.5%;vitamin B1268%;folic acid 25.5%)and over 50%of women had two or more concurrent deficiencies as well as multiple *** regression models highlighted differences in variables associated with CRP between pregnancy and *** pregnancy,CRP was positively associated with greater indoor wood smoke exposure,caries and hookworm and negatively associated with Ascaris and vaginal Lactobacillus and Bacteroides/Gardnerella *** with this,greater wood smoke exposure,caries as well as higher diplococcal infection score increased the odds of trimester-elevated CRP concentrations whereas longer gestational age lowered the likelihood of a trimester-elevated *** lactation,folic acid deficiency was associated with higher CRP whereas parity,number of eosinophils and Mobiluncus score were associated with lower ***,a higher BMI and Trichomonas vaginalis score increased the likelihood of an elevated CRP whereas higher parity and numbe

关键词： CRP Intestinal parasites Caries Vaginal infections Folic acid deficiency Wood smoke Neutrophil/lymphocyte ratio Plateletcrit Pregnancy Lactation

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