Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori
作者机构：Departamento de BiotecnologiaGenetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y FibrasHernandariasParaguay Universidade Estadual do Oeste do ParanaCascavelBrasil
出 版 物：《Open Journal of Genetics》 (遗传学期刊（英文）)
年 卷 期：2014年第4卷第5期
页 面：370-377页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by CAPES CNPq FINEP/Fundacao Araucaria and Secretaria de Estado da Ciencia Tecnologia e Ensino Superior—FUNDO PARANA
主 题：Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori
摘 要：This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.
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