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Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4

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The Crop Journal 2024年第1期12卷 188-201页

作者： Guoliang Ma Mingzhuo Li Yingling Wu Changjuan Jiang Yifan Chen Dawei Xing Yue Zhao Yajun Liu Xiaolan Jiang Tao Xia Liping Gao school of Life Science Anhui Agricultural UniversityHefei 230036AnhuiChina Plant for Human Health Institute Department of Plant and Microbial BiologyNorth Carolina State UniversityKannapolisNC 28081USA State Key Laboratory of tea Plant Biology and Utilization/Key Laboratory of tea Biology and tea Processing of Ministry of Agriculture/Anhui Provincial Laboratory of tea Plant Biology and Utilization Anhui Agricultural UniversityHefei 230036AnhuiChina

Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in plants.Our previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained unknown.Here,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament growth.Transcriptome analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal pathway.Auxin/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as bait.Gene silencing assays showed that both CsIAA4 and CsMYB4a regulate filament growth.Tobacco plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of CsMYB4a.Protein-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 degradation.Knock out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing CsMYB4a.All results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.

关键词： AUX/IAA4 Auxin signaling CsMYB4a Subgroup 4 R2R3-MYB

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Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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Food Science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong Jing Xie Liang Tao Jing-Jing Dai Tingting Li Li Zhang Yuying Bai Xia Hu Jinlian Chen Jun Sheng Yang Tian College of Food Science and Technology Yunnan Agricultural UniversityKunming 650201China Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China school of tea and coffee Pu’er UniversityPu’er 665000China Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Pu-er tea Science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

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Distinct Effects of Three tea Varieties Made Green teas on Improving Diabetic Dyslipidemia Induced by High-Fat Diet in C57BL/6J Mice

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Food Science and Human Wellness 2024年

作者： Wei Gu Zhutong Wang Guohuo Wu Huimin Guo Huijun Cheng Cong Li Linbo Chen Daxiang Li Zhongwen Xie State Key Laboratory of tea Plant Biology and Utilization school of tea and Food Sciences & Technology Anhui Agricultural University Center for Biotechnology Anhui Agricultural University College of Life Sciences Anhui Agricultural University tea Research Institute Yunnan Academy of Agricultural Sciences

Currently， cultivated tea plants are classified into three varieties， namely， C. sinensis var. sinensis， C. sinensis var. assamica， and C. sinensis var. kucha. Three tea varieties made green teas (TVGTs) are widely consumed worldwide. However， a comparative investigation of the beneficial effects， mechanism， and characteristic compounds of TVGTs has not been reported. Here， three representative tea plant varieties， namely， C. sinensis var. sinensis cv. Fuding (FD)， C. sinensis var. assamica cv. Yunkang 10 (YK)， and C. sinensis var. kucha (KC)， planted in the same garden， were used to make green teas utilizing the same standard procedure. Our findings show that the TVGTs effectively improved hyperglycemia， obesity， dyslipidemia， fatty liver， and mesenteric artery (MA) hypercontractility in mice with diabetes and obesity induced by a high-fat diet (HFD) after 11- and 22-week interventions， with YK being the most effective at 22 weeks. Liver lipidomics indicated that the TVGTs restored the glycerophospholipid and sphingolipid balance and reduced triglycerides， with YK demonstrating superior effects. The TVGTs， particularly YK， suppressed triglyceride synthesis through the ARV1-FXR-SHP-SREBP-1c pathway. A chemical profile analysis revealed that YK green tea had enriched active compounds， including caffeine， quinic acid， L-theanine， gallic acid， and catechins， compared with FD and KC green teas， which may contribute to ameliorating diabetic dyslipidemia via synergistic action. As a representative tea variety of C. sinensis var. assamica， YK green tea was found to be superior to the other two green teas in preventing and treating diabetic dyslipidemia， especially with long-term consumption. These data provide important information for tea plant breeders and tea consumers.

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Multi-Scale Mixed Attention tea Shoot Instance Segmentation Model

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 261-275页

作者： Dongmei Chen Peipei Cao Lijie Yan Huidong Chen Jia Lin Xin Li Lin Yuan Kaihua Wu school of Automation Hangzhou Dianzi UniversityHangzhou310018China tea Research Institute Chinese Academy of Agriculture SciencesHangzhou310018China school of Information Engineering Zhejiang University of Water Resources and Electric PowerHangzhou310018China

tea leaf picking is a crucial stage in tea production that directly influences the quality and value of the tea.Traditional tea-picking machines may compromise the quality of the tea leaves.High-quality teas are often handpicked and need more delicate operations in intelligent picking machines.Compared with traditional image processing techniques,deep learning models have stronger feature extraction capabilities,and better generalization and are more suitable for practical tea shoot harvesting.However,current research mostly focuses on shoot detection and cannot directly accomplish end-to-end shoot segmentation tasks.We propose a tea shoot instance segmentation model based on multi-scale mixed attention(Mask2FusionNet)using a dataset from the tea garden in Hangzhou.We further analyzed the characteristics of the tea shoot dataset,where the proportion of small to medium-sized targets is 89.9%.Our algorithm is compared with several mainstream object segmentation algorithms,and the results demonstrate that our model achieves an accuracy of 82%in recognizing the tea shoots,showing a better performance compared to other models.Through ablation experiments,we found that ResNet50,PointRend strategy,and the Feature Pyramid Network(FPN)architecture can improve performance by 1.6%,1.4%,and 2.4%,respectively.These experiments demonstrated that our proposed multi-scale and point selection strategy optimizes the feature extraction capability for overlapping small targets.The results indicate that the proposed Mask2FusionNet model can perform the shoot segmentation in unstructured environments,realizing the individual distinction of tea shoots,and complete extraction of the shoot edge contours with a segmentation accuracy of 82.0%.The research results can provide algorithmic support for the segmentation and intelligent harvesting of premium tea shoots at different scales.

关键词： tea shoots attention mechanism multi-scale feature extraction instance segmentation deep learning

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On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay

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Journal of Pharmaceutical Analysis 2024年第2期14卷 276-283页

作者： Junxia Gao Tianyi Zhang Yihua Fang Ying Zhao Mei Yang Li Zhao Ye Li Jun Huang Guonian Zhu Yirong Guo Institute of Pesticide and Environmental Toxicology Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang ProvinceMinistry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and InsectsZhejiang UniversityHangzhou310058China tea Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Tea Biology and Resources UtilizationMinistry of AgricultureHangzhou310008China Shanghai Agricultural Technology Extension Service Center Shanghai201103China school of Biological and Chemical Engineering Zhejiang University of Science and TechnologyHangzhou311200China

The application of pesticides (mostly insecticides and fungicides) during the tea-planting process will undoubtedly increase the dietary risk associated with drinking tea. Thus, it is necessary to ascertain whether pesticide residues in tea products exceed the maximum residue limits. However, the complex matrices present in tea samples comprise a major challenge in the analytical detection of pesticide residues. In this study, nine types of lateral flow immunochromatographic strips (LFICSs) were developed to detect the pesticides of interest (fenpropathrin, chlorpyrifos, imidacloprid, thiamethoxam, acetamiprid, carbendazim, chlorothalonil, pyraclostrobin, and iprodione). To reduce the interference of tea substrates on the assay sensitivity, the pretreatment conditions for tea samples, including the extraction solvent, extraction time, and purification agent, were optimized for the simultaneous detection of these pesticides. The entire testing procedure (including pretreatment and detection) could be completed within 30 min. The detected results of authentic tea samples were confirmed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which suggest that the LFICS coupled with sample rapid pretreatment can be used for on-site rapid screening of the target pesticide in tea products prior to their market release.

关键词： Lateral flow immunoassay Rapid detection Pesticide multi-residue tea matrix Sample rapid pretreatment

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The MtRGF6 Peptide Differentially Regulates Root Development and Symbiotic Nodulation of Medicago truncatula and Lotus japonicus

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1237-1248页

作者： Junhui Yan Yawen Wang Qiong Li Yu Zhou Xu Wang Li Luo school of Life Sciences Shanghai Key Laboratory of Bio-Energy CropsShanghai UniversityShanghai200444China College of Life Science State Key Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefei230036China

Rhizobia induces nitrogen-ﬁxing nodules in legumes used in agricultural production,providing a direct source of combined nitrogen to leguminous crops.Small peptides,such as CLAVATA3/EMBRYO SURROUNDING REGION peptides(CLE),are known to regulate the formation and development of nitrogen-ﬁxing nodules in legumes.Root meristem growth factor(RGF)peptides from Medicago truncatula not only regulate root develop-ment but also modulate nodulation symbiosis with Sinorhizobium meliloti.However,the impact of RGF peptides from one leguminous species on the others remains unclear.In this study,we investigate the effects of the RGF family peptide MtRGF6p from M.truncatula on nodulation symbiosis and root development in Lotus japonicus.The MtRGF6 gene is predominantly expressed in the root nodules of M.truncatula and shows low identity with RGF homologous genes from L.japonicus.The gene promoter is active in the primordia of root nodules and lat-eral roots,as well as in young nodules and roots,and the meristem,infection,and nitrogen-ﬁxing regions of the mature nodule.Chemically synthesized MtRGF6p promoted primary root growth in M.truncatula but sup-pressed the growth of L.japonicus primary roots.The peptide negatively affected the initiation of nodule primor-dia,the formation of infection threads,and nodulation in both legumes,with a low dosage showing effects on L.japonicus compared to M.truncatula.These results suggest that the MtRGF6 peptide from M.truncatula may serve as an inter-species signal affecting the root organ development of L.japonicus.

关键词： Peptide RGF legume symbiotic nodulation lateral root

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Aging-induced YTHDF aggregates impair mitochondrial function by trapping mitochondrial RNAs and suppressing their expression in the brain

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Protein & Cell 2024年第2期15卷 149-155页

作者： Juan Zhang Dingfeng Li Keqiang He Qiang Liu Zhongwen Xie school of Life Sciences Anhui Agricultural UniversityHefei 230036China State Key Laboratory of tea Plant Biology and Utilization School of Tea and Food Sciences and TechnologyAnhui Agricultural UniversityHefei230036China Division of Life Sciences and Medicine Hefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Department of Anesthesiology The First Affliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Anhui Province Key Laboratory of Biomedical Aging Research University of Science and Technology of ChinaHefei 230026China Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China

DearEditor,Brain aging is associated with a decrease in cognitive function,which is often accompanied by defective changes in mitochondrial morphology and reduction in mitochondrial function(Faitg et al.,2021).The main function of mitochondria is to produce energy in the form of ATP,which is vital to fulfill high energy demand of neurons,mainly for synaptic processes(Lee et al.,2018).Mitochondrial dysfunction acts as a key factor for age-related cognitive decline(Grimm and Eckert,2017).

关键词： function trapping

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Polysaccharides of Aspergillus cristatus attenuate obesity by regulating gut microbiota and gut microbiota-related metabolites

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Food Science and Human Wellness 2024年第3期13卷 1513-1530页

作者： Mingzhi Zhu Bohao Shang Fang Zhou Yong Yuan Feiyan Yin Jin Cao Jianan Huang Kunbo Wang Xin Zeng Maiquan Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China College of Life Sciences Huaibei Normal UniversityHuaibei 235000China Hunan tea Group Co. Ltd.Changsha 410128China school of Food Science and Technology Hunan Agricultural UniversityChangsha 410128China

Golden-flower fungus,the only dominant microorganism determining the Fu-brick tea quality through fermentation and the important microbe in Liupao tea,is considered a potential probiotic fungus based on its anti-obesity effect.However,the classification of golden-flower fungi is still controversial;the anti-obesity effect of golden-flower fungus polysaccharides remains unknown.In this study,we identify a golden-flower strain as Aspergillus cristatus based on morphological characteristics and multigene phylogeny analysis,which resolves the controversy of classification.Moreover,we find A.cristatus polysaccharides(ACPS)attenuate obesity in rats.ACPS modulate gut bacterial composition.in which Akkermansia,Akkermansia muciniphila,Bacteroides,Romboutsia,Blautia,and Desulfovibrio are considered the core microbes regulated by ACPS.ACPS increase fecal total short-chain fatty acid content and serum,hepatic,and fecal total bile acid content.Furthermore,ACPS-induced gut microbiota alteration plays a causal role in the protection from obesity,according to a fecal transplantation experiment.Thus,ACPS ameliorate obesity by regulating gut microbiota and gut microbiota-related metabolites.

关键词： Golden-flower fungus Aspergillus cristatus Polysaccharides Obesity Gut microbiota

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Duplicated chalcone synthase(CHS)genes modulate flavonoid production in tea plants in response to light stress

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Journal of Integrative Agriculture 2024年第6期23卷 1940-1955页

作者： Mingzhuo Li Wenzhao Wang Yeru Wang Lili Guo Yajun Liu Xiaolan Jiang Liping Gao Tao Xia State Key Laboratory of tea Plant Biochemistry and Utilization Anhui Agricultural UniversityHefei 230036China school of Life Sciences Anhui Agricultural UniversityHefei 230036China College of Horticulture Northwest A&F UniversityYangling 712100China Department of Plant and Microbial Biology North Carolina State UniversityRaleigh 27606NCUSA

In tea plants,the abundant flavonoid compounds are responsible for the health benefits for the human body and define the astringent flavor profile.While the downstream mechanisms of flavonoid biosynthesis have been extensively studied,the role of chalcone synthase(CHS)in this secondary metabolic process in tea plants remains less clear.In this study,we compared the evolutionary profile of the flavonoid metabolism pathway and discovered that gene duplication of CHS occurred in tea plants.We identified three CsCHS genes,along with a CsCHS-like gene,as potential candidates for further functional investigation.Unlike the CsCHS-like gene,the CsCHS genes effectively restored flavonoid production in Arabidopsis chs-mutants.Additionally,CsCHS transgenic tobacco plants exhibited higher flavonoid compound accumulation compared to their wild-type counterparts.Most notably,our examination of promoter and gene expression levels for the selected CHS genes revealed distinct responses to UV-B stress in tea plants.Our findings suggest that environmental factors such as UV-B exposure could have been the key drivers behind the gene duplication events in CHS.

关键词： tea flavonoids biosynthesis CHS gene duplication UV-B stress

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Association analysis of an anti-obesity mechanism and key ripened Pu-erh tea bioactive components by mimicking human general tea drinking

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Food Science and Human Wellness 2024年

作者： Junyu Liu Zhengyang Song Haihong Chen Wen Zeng Guirong Han Wei Li Bing Xu Yuan Lu Canyang Zhang Zhenglian Xue Bin Lü Chong Zhang Song Yang Yi Wang Xinhui Xing Institute of Biomedical Health Technology and Engineering Shenzhen Bay Laboratory MOE Key Laboratory for Industrial Biocatalysis Centre for Synthetic and Systems Biology Department of Chemical Engineering Tsinghua University Shenzhen International Graduate school Tsinghua University Shandong Province Key Laboratory of Applied Mycology Qingdao International Center on Microbes Utilizing Biogas school of Life Sciences Qingdao Agricultural University Anhui University of Technology Yunnan Shujian tea Co. Ltd.

Pu-erh tea， a traditional Chinese beverage， performs an anti-obesity function， but the correlation between its components and efficacy remains unknown. Here， we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas. In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by tea-B than tea-A. Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas. tea-A changes the histomorphology of brown adipose tissue (BAT) and increases the abundance of Coriobacteriaceae＿UCG＿002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane， decamethyl， tridecane and 1，2，3-trimethoxybenzene， eventually increasing BAT activation and fat browning gene expression; the high content of hexadecane and 1，2-dimethoxy-benzene in tea-B reduces white adipose tissue (WAT) accumulation and the process of fatty liver， increases the abundance of Odoribacter and sphinganine 1-phosphate， inhibits the expression of lipid synthesis and transport genes. These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes， gut microbes， gut metabolite structure and anti-obesity pathways， which were obtained for the first time， provide foundations for developing functional Pu-erh tea.

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