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Generation of Eco-Friendly and Disease-Resistant Channel Catfish(Ictalurus punctatus)Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai Wang Baofeng Su De Xing Timothy J.Bruce Shangjia Li Logan Bern Mei Shang Andrew Johnson Rhoda Mae C.Simora Michael Coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin Jacob Al-Armanazi Cuiyu Lu Rex A.Dunham school of fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the CRISPR/Cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-Cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-Cath was instrumental in establishing the lh knockout(LH^(–)_As-Cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HCG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture

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Full sexual maturity-cycle exposure to environmentally relevant concentrations of 17β-estradiol decreases reproductive capacity of zebrafish

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Journal of Environmental Sciences 2024年第3期137卷 580-592页

作者： Yongkang Zhang Yuanjun Jiang Qian Sun Meng Li Sheng Chen Jingyu Liang Chunsheng Liu College of fisheries Huazhong Agricultural UniversityWuhan 430070China school of Environmental Studies China University of GeosciencesWuhan 430074China

17β-Estradiol(E2)has been widely detected in natural water and treatment with E2 induces potential endocrine disrupting effects in ***,effects on fish fecundity and steroid system after treatment with environmentally relevant concentrations of E2 for the full sexual maturation cycle remain *** this study,zebrafish were treatment with 0,10 or100 ng/L E2 from embryo to adult stage,and effects on gonadal development and differentiation,steroid hormone levels,transcription of genes associated with the hypothalamic-pituitary-gonadal-liver(HPG)axis in adults and fertilization rate of offspring were *** results showed that treatment with E2 lead to increased number of feminization in *** females,E2 decreased cumulative amount of spawning and inhibited the maturation of *** males,E2 inhibited the maturation and motility of sperm,as well as decreased the movement speed of *** adverse effects on sperm might be responsible for the reduced fertilization observed in *** addition,treatment with E2 changed the levels of steroid hormones in zebrafish gonad and altered the transcriptional levels of genes associated with HPG axis,which is responsible for the regulation of germ cells maturation and gonadal development in ***,these results suggested that treatment with environmentally relevant concentrations of E2 for the full sexual maturity cycle resulted in adverse effects on reproduction in zebrafish.

关键词： Environmental estrogen Zebrafish Fecundity Gonadal development Endocrine disruption

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Flow Field Characteristics of Multi-Trophic Artificial Reef Based on Computation Fluid Dynamics

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Journal of Ocean University of China 2024年第2期23卷 317-327页

作者： HUANG Junlin LI Jiao LI Yan GONG Pihai GUAN Changtao XIA Xu school of Engineering Ocean University of ChinaQingdao 266100China Yellow Sea fisheries Research Institute Chinese Academy of Fishery SciencesKey Laboratory of Sustainable Development of Marine FisheriesMinistry of Agriculture and Rural AffairsQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyQingdao 266071China Laboratory for Marine fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China

On the basis of computational fluid dynamics,the flow field characteristics of multi-trophic artificial reefs,including the flow field distribution features of a single reef under three different velocities and the effect of spacing between reefs on flow scale and the flow state,were *** indicate upwelling,slow flow,and eddy around a single *** velocity,height,and volume of upwelling in front of a single reef were positively correlated with inflow *** length and volume of slow flow increased with the increase in inflow *** were present both inside and backward,and vorticity was positively correlated with inflow *** between reefs had a minor influence on the maximum velocity and height of *** the increase in space from 0.5 L to 1.5 L(L is the reef lehgth),the length of slow flow in the front and back of the combined reefs increased *** the space was 2.0 L,the length of the slow flow *** four different spaces,eddies were present inside and at the back of each *** maximum vorticity was negatively correlated with space from 0.5 L to 1.5 L,but under 2.0 L space,the maximum vorticity was close to the vorticity of a single reef under the same inflow velocity.

关键词： artificial reef flow field characteristics computation fluid dynamics multi-trophic structure

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Spatiotemporal Distributions of Bacterioplankton Communities in the Qiantang River(Hangzhou Section),China

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Journal of Ocean University of China 2024年第4期23卷 1102-1114页

作者： XU Jiaojiao ZHAO Li LUKWAMBE Betina NICHOLAUS Regan ZHU Jinyong YANG Wen ZHENG Zhongming school of Marine Sciences Ningbo UniversityNingbo 315211China school of Aquatic Sciences and fisheries Technology University of Dar es SalaamDar es SalaamTanzania Department of Natural Sciences Mbeya University of Science and TechnologyMbeyaTanzania

The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic *** this study,we conducted a comprehensive investigation of the bacterioplankton communities in the Qiantang River(Hangzhou section).Water samples were collected quarterly from seven sites over a one-year *** and chemical parameters,including dissolved oxygen(DO),water temperature(WT),chemical oxygen demand(COD),nitrite(NO_(2)^(-)),active phosphate(PO_(4)^(3-))and other indices were *** this study,theαdiversity,βdiversity and abundance differences of bacterial communities were investigated using 16S rRNA high-throughput sequencing *** spatial and temporal distribution characteristics and main driving factors of the bacterioplankton community structure and diversity were *** results showed that a total of 57 phyla were detected in the bacterioplankton community,among which Proteobacteria and Actinomycetes were the two dominant groups with the highest relative *** results of PCoA based on Bray-Curtis distance showed that the sampling season had a slightly greater effect on the changes in bacterioplankton community structure in the Qiantang *** and partial Mantel test showed that environmental variables(Mantel r=0.6739,P<0.0001;partial Mantel r=0.507,P=0.0001)were more important than geographical distance(Mantel r=0.5322,P<0.001;partial Mantel r=0.1563,P=0.001).The distance attenuation model showed that there was significant distance attenuation in all four seasons,and the maximum limit of bacterial community diffusion was found in *** analysis showed that nine environmental factors,including COD,WT and DO,significantly affected community distribution(P<0.05).This study provides valuable insights into the spatial and temporal distribution characteristics of bacterioplankton communities,shedding light on their ecological roles in the Qiantang R

关键词： Qiantang River bacterioplankton community spatial and temporal distribution 16S rRNA range attenuation

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Effects of shore-normal coastal structure on medium-to long-term embayed shoreline evolution

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Acta Oceanologica Sinica 2024年第2期43卷 58-66页

作者： Jitao Yu Yuanting Ding Pei Liu Renfu Fan Lin Zhang Hainan Academy of Ocean and fisheries Sciences Haikou 571126China Yazhou Bay Innovation Institute Hainan Tropical Ocean UniversitySanya 572025China school of Geography and Environmental Sciences Hainan Normal UniversityHaikou 571158China

Based on high-tide shoreline data extracted from 87 Landsat satellite images from 1986 to 2019 as well as using the linear regression rate and performing a Mann-Kendall(M–K)trend test,this study analyzes the linear characteristics and nonlinear behavior of the medium-to long-term shoreline evolution of Jinghai Bay,eastern Guangdong *** particular,shoreline rotation caused by a shore-normal coastal structure is *** results show that the overall shoreline evolution over the past 30 years is characterized by erosion on the southwest beach,with an average erosion rate of 3.1 m/a,and significant accretion on the northeast beach,with an average accretion rate of 5.6 m/*** of the M–K trend test indicate that significant shoreline changes occurred in early 2006,which can be attributed to shore-normal *** to that engineering construction,the shorelines are slightly eroded,where the average erosion rate is 0.7 m/***,after shore-normal engineering is performed,the shoreline is characterized by significant erosion(3.2 m/a)on the southwest beach and significant accretion(8.5 m/a)on the northeast beach,thus indicating that the shore-normal engineering at the updrift headland contributes to clockwise shoreline *** analysis shows that the clockwise shoreline rotation is promoted not only by longshore sediment transport processes from southwest to northeast,but also by cross-shore sediment transport *** findings are crucial for beach erosion risk management,coastal disaster zoning,regional sediment budget assessments,and further observations and predictions of beach morphodynamics.

关键词： shoreline rotation non-linear behavior longshore sediment transport cross-shore sediment transport embayed beach coastal structure

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Zoonotic human liver flukes, a type 1 biocarcinogen, in freshwater fishes: genetic analysis and confirmation of molluscan vectors and reservoir hosts in Bangladesh

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贫困所致传染病(英文) 2024年第3期13卷 73-88页

作者： Labony Sharmin Shahid Alim Md. Abdul Hasan Muhammad Mehedi Hossain Md. Shahadat Akter Sharmin Paul Joydeep Farjana Thahsin Ali Md. Haydar Alam Mohammad Zahangir Hatta Takeshi Kawada Hayato Mizutani Keiko Tsuji Naotoshi Anisuzzaman Department of Parasitology Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh Department of fisheries Technology Faculty of Fisheries Bangladesh Agricultural University Mymensingh Bangladesh Department of Biotechnology School of Life Sciences &amp Biotechnology Adamas University Barasat - Barrackpore Rd Jagannathpur Kolkata West Bengal India Department of Pathology and Parasitology Hajee Mohammad Danesh Science and Technology University Dinajpur Bangladesh Department of Parasitology and Tropical Medicine Kitasato University School of Medicine-15-Kitasato Minami Sagamihara Kanagawa-0374 Japan Department of Molecular and Cellular Parasitology Kitasato University Graduate School of Medical Sciences-15-Kitasato Minami Sagamihara Kanagawa-0373 Japan

Background Opisthorchiid flukes, particularlyOpisthorchis viverrini,Opisthorchis felineus,Clonorchis sinensis, andMetorchis spp. are the most common fish-borne zoonotic human liver flukes (hLFs). Liver fluke infections are more prevalent in resource-deprived and underprivileged areas. We herein estimated the prevalence of the metacercariae (MC) of major hLFs in common large freshwater fishes (lFWF) marketed for human consumption from some selected areas of Bangladesh along with detection of their molluscan vectors and *** The current status of fish-borne zoonotic hLF infections in lFWF was investigated along with their molluscan vectors and mammalian reservoir hosts in Mymensingh and Kishoreganj in Bangladesh from July 2018–June 2022 using conventional and multiple molecular techniques, such as PCR, PCR-restriction fragment length polymorphism (RFLP), sequencing, and bioinformatic analyses. The infection rate of fishes was analyzed using theZ-test and the loads of MC were compared using the chi-squared (χ2) *** The MC ofC. sinensis,Opisthorchis spp., andMetorchis spp. were detected in 11 species of common and popular lFWF. In lFWF, the estimated prevalence was 18.7% and the mean load was 137.4 ± 149.8 MC per 100 g of fish. The prevalence was the highest (P 0.05) distributed between the head and body of fishes. The infection rate was slightly higher in cultured (19.6%) fishes. The MC ofC. sinensis,O. felineus,O. viverrini, andMetorchis orientalis in fishes were confirmed using PCR, PCR-RFLP and bioinformatics. The cercariae of opisthorchiid (Pleurolophocercus cercariae) flukes were only recovered fromBithynia spp. (3.9%, 42 out of 1089). The ova of hLFs from dogs (4.3%, 5 out of 116) and cats (6.0%, 6 out of 100), and adult flukes (***) from ducks

关键词： Human liver fluke Freshwater fish Molluscan vector Reservoir host Bangladesh

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Selected dietary plant-based proteins for growth and health response of Nile tilapia Oreochromis niloticus

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Aquaculture and fisheries 2024年第1期9卷 3-19页

作者： Tzodoq Obrero Magbanua Janice Alano Ragaza Ateneo Aquatic and fisheries Resources Laboratory Department of BiologySchool of Science and EngineeringAteneo de Manila UniversityKatipunan AvenueLoyola HeightsQuezon CityMetro Manila1108Philippines

Tilapia culture is one of the largest sectors of global *** the different species of tilapia,Nile tilapia(Oreochromis niloticus)is perhaps the top cultured *** production of Nile tilapia has been continually increasing throughout the years resulting in genetic *** tilapia strains with better growth performance and adaptive capability to survive in different culture conditions have been developed to alleviate the *** demand for Nile tilapia implies higher farming ***-based proteins are utilized as partial or complete fishmeal replacements to reduce feed ***,these proteins can adversely affect and alter growth and feed performance,carcass composition and indices,and gut and hepatic *** review discusses the use of seven plant-based proteins:namely,soybean,copra,pea,corn,palm kernel,microalgae,and seaweed as a Nile tilapia *** processing methods are employed to produce several types of plant-based *** plant-protein types,when utilized as an aquafeed ingredient,vary in its effect on the performance,hemato-immunological parameters,and gut and hepatic health of Nile *** have shown that Nile tilapia can effectively maximize plant-based protein diets based on the preparation method,type of plant source,amino acid supplementation,and inclusion levels of the plant *** readily available crops should be considered as primary protein sources for *** to the use of plant-based proteins as a main dietary protein are limiting amino acids,presence of anti-nutritional factors,and the competition between its demand as human food and as animal feed.

关键词： Nile tilapia Plant-based proteins Soybean proteins Copra proteins Pea proteins Corn proteins Palm kernel proteins Algal proteins

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Nano polystyrene microplastics could accumulate in Nile tilapia(Oreochromis niloticus):Negatively impacts on the intestinal and liver health through water exposure

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Journal of Environmental Sciences 2024年第3期137卷 604-614页

作者： Di Wu Xing Lu Li-Xue Dong Juan Tian Jin Deng Lei Wei Hua Wen Shan Zhong Ming Jiang school of Basic Medical Sciences Wuhan UniversityWuhan 430071China Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural AffairsYangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhan 430223China Hubei Province Key Laboratory of Allergy and Immunology Wuhan 430071China

Microplastics(MPs)have become a significant concern for their potential ***,the correlation between the size of plastic particles and their toxicity remains ***,we investigate the toxic effects of different sizes(80 nm,800 nm,8μm and 80μm)polystyrene MPs(PS-MPs)on the model organism Nile tilapia(Oreochromis niloticus).The results of bioluminescent imaging indicate that the 80 nm PS-MPs are more likely to invade the body.H&E staining shows severe damage on the intestinal villi and distinct hepatic steatosis in the 80 nm *** U labeling shows that the proliferation activity of intestinal and liver cells reduces significantly in the 80 nm *** gut microbiome analysis shows a severe imbalance of gut microbiota homeostasis in the 80 nm *** analysis of liver transcriptomics and metabolomics shows that the liver lipid metabolism is disordered in the 80 nm *** conclusion,this study confirms that the 80 nm PS-MPs are more likely to induce intestinal and liver *** the above lay the foundation for further study on the pathological damage of MPs to other organisms.

关键词： Microplastics(MPs) Intestinal toxicity Liver toxicity Gut microbiome Lipid metabolism

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Cell metabolism pathways involved in the pathophysiological changes of diabetic peripheral neuropathy

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Neural Regeneration Research 2024年第3期19卷 598-605页

作者： Yaowei Lv Xiangyun Yao Xiao Li Yuanming Ouyang Cunyi Fan Yun Qian Department of Orthopedics Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration ShanghaiChina Youth Science and Technology Innovation Studio of Shanghai Jiao Tong University school of Medicine ShanghaiChina College of fisheries and Life Science Shanghai Ocean UniversityShanghaiChina

Diabetic peripheral neuropathy is a common complication of diabetes *** the pathophysiological metabolic mechanism impels the generation of ideal ***,existing limited treatments for diabetic peripheral neuropathy expose the urgent need for cell metabolism *** the lack of comprehensive understanding of energy metabolism changes and related signaling pathways in diabetic peripheral neuropathy,it is essential to explore energy changes and metabolic changes in diabetic peripheral neuropathy to develop suitable treatment *** review summarizes the pathophysiological mechanism of diabetic peripheral neuropathy from the perspective of cellular metabolism and the specific interventions for different metabolic pathways to develop effective treatment *** metabolic mechanisms(e.g.,polyol,hexosamine,protein kinase C pathway)are associated with diabetic peripheral neuropathy,and researchers are looking for more effective treatments through these pathways.

关键词： cell metabolism diabetic peripheral neuropathy peripheral nerve injury protein kinase C pathway reactive oxygen species.

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Bactericidal ability of target acidic phospholipids and phagocytosis of CDC42 GTPase-mediated cytoskeletal rearrangement underlie functional conservation of CXCL12 in vertebrates

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Science China Life Sciences 2024年

作者： Yanqi Zhang Ning Xia Yazhen Hu Wentao Zhu Chunrong Yang Jianguo Su Hubei Hongshan Laboratory College of Fisheries Huazhong Agricultural University Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center school of Marine Sciences Ningbo University College of Veterinary Medicine Huazhong Agricultural University

Chemokine CXCL12 plays a crucial role in both direct bactericidal activity and phagocytosis in humans. However, the mechanisms and evolutionary functions of these processes in vertebrates remain largely unknown. In this study, we found that the direct bactericidal activity of CXCL12 is highly conserved across various vertebrate lineages, including Arctic lamprey (Lampetra japonica), Basking shark (Cetorhinus maximus), grass carp (Ctenopharyngodon idella), Western clawed frog (Xenopus tropicalis), Green anole (Anolis carolinensis), chicken(Gallus gallus), and human (Homo sapiens). CXCL12 also has been shown to promote phagocytosis in lower and higher vertebrates. We then employed C. idella CXCL12a (CiCXCL12a) as a model to further investigate its immune functions and underlying mechanisms. CiCXCL12a exerts direct broad-spectrum antibacterial activity by targeting bacterial acidic phospholipids, resulting in bacterial cell membrane perforation, and eventual lysis. Monocytes/macrophages are attracted to the infection sites for phagocytosis through the rapid production of CiCXCL12a during bacterial infection. CiCXCL12a induces CDC42 and CDC42 GTPase activation, which in turn mediates F-actin polymerization and cytoskeletal rearrangement. The interaction between F-actin and Aeromonas hydrophila facilitates bacterial internalization into monocytes/macrophages. Additionally, A. hydrophila is colocalized within early endosomes, late endosomes and lysosomes, ultimately degrading within phagolysosomes. CiCXCL12a also activates PI3K-AKT, JAK-STAT5 and MAPK-ERK signaling pathways. Notably, only the PI3K-AKT signaling pathway inhibits LPS-induced monocyte/macrophage apoptosis. Thus, CiCXCL12a plays key roles in reducing tissue bacterial loads, attenuating organ injury, and decreasing mortality rates. Altogether, our findings elucidate the conserved mechanisms underlying CXCL12-mediated bactericidal activity and phagocytosis, providing novel perspectives into the immune functions

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