Zoonotic human liver flukes,a type 1 biocarcinogen,in freshwater fishes:genetic analysis and confirmation of molluscan vectors and reservoir hosts in Bangladesh

作     者：Sharmin Shahid Labony Md.Abdul Alim Muhammad Mehedi Hasan Md.Shahadat Hossain Sharmin Akter Joydeep Paul Thahsin Farjana Md.Haydar Ali Mohammad Zahangir Alam Takeshi Hatta Hayato Kawada Keiko Mizutani Naotoshi Tsuji null Anisuzzaman 

作者机构：Department of ParasitologyFaculty of Veterinary ScienceBangladesh Agricultural UniversityMymensingh 2202Bangladesh Department of Fisheries TechnologyFaculty of FisheriesBangladesh Agricultural UniversityMymensingh 2202Bangladesh Department of BiotechnologySchool of Life Sciences&BiotechnologyAdamas UniversityBarasat-Barrackpore RdJagannathpurKolkataWest Bengal 700126India Department of Pathology and ParasitologyHajee Mohammad Danesh Science and Technology UniversityDinajpur 5200Bangladesh Department of Parasitology and Tropical MedicineKitasato University School of Medicine1-15-1 KitasatoMinamiSagamiharaKanagawa 252-0374Japan Department of Molecular and Cellular ParasitologyKitasato University Graduate School of Medical Sciences1-15-1 KitasatoMinamiSagamiharaKanagawa 252-0373Japan 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2024年第13卷第3期

页      面：73-88页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：funded by the Ministry of Education(MoE)and Ministry of Science and Technology(MoST),The Government of Bangladesh supported by the JSPS Core-to-Core Program(JSJSCCB20200010) 

主　　题：Human liver fluke Freshwater fish Molluscan vector Reservoir host Bangladesh 

摘      要：Background Opisthorchiid flukes,particularly Opisthorchis viverrini,Opisthorchis felineus,Clonorchis sinensis,and Metorchis *** the most common fish-borne zoonotic human liver flukes(hLFs).Liver fluke infections are more prevalent in resource-deprived and underprivileged *** herein estimated the prevalence of the metacercariae(MC)of major hLFs in common large freshwater fishes(lFWF)marketed for human consumption from some selected areas of Bangladesh along with detection of their molluscan vectors and *** The current status of fish-borne zoonotic hLF infections in lFWF was investigated along with their molluscan vectors and mammalian reservoir hosts in Mymensingh and Kishoreganj in Bangladesh from July 2018-June 2022 using conventional and multiple molecular techniques,such as PCR,PCR-restriction fragment length polymorphism(RFLP),sequencing,and bioinformatic *** infection rate of fishes was analyzed using the Z-test and the loads of MC were compared using the chi-squared(χ^(2))*** The MC of ***,Opisthorchis spp.,and Metorchis *** detected in 11 species of common and popular *** lFWF,the estimated prevalence was 18.7%and the mean load was 137.4±149.8 MC per 100 g of *** prevalence was the highest(P0.05)distributed between the head and body of *** infection rate was slightly higher in cultured(19.6%)*** MC of ***,***,***,and Metorchis orientalis in fishes were confirmed using PCR,PCR-RFLP and *** cercariae of opisthorchiid(Pleurolophocercus cercariae)flukes were only recovered from Bithynia spp.(3.9%,42 out of 1089).The ova of hLFs from dogs(4.3%,5 out of 116)and cats(6.0%,6 out of 100),and adult flukes(***)from ducks(41.1%113 out of 275)were *** The MC of