检索结果-南通市图书馆

Apoptosis induction of colorectal cancer cells HTL-9 in vitro by the transformed products of soybean isoflavones by Ganoderma lucidum

引用

Journal of zhejiang university-science B(Biomedicine & Biotechnology) 2017年第12期18卷 1101-1112页

作者： Mei-lin CUI Huan-yi YANG Guo-qing HE College of food science Shanxi Normal University Linfen 041004 China College of biosystems engineering and food science Zhejiang University Hangzhou 310058 China zhejiang Provincial Key Laboratory of food Microbiology Zhejiang University Hangzhou 310058 China

Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of Ganoderma lucidum. The resultant transformed products（TSI） contained（703.21±4.35） mg/g of genistein, with transformed rates of 96.63% and 87.82% of daidzein and genistein, respectively, and TSI also could enrich the bioactive metabolites of G. lucidum. The antitumor effects of TSI on human colorectal cancer cell line HTL-9, human breast cancer cell line MCF-7, and human immortalized gastric epithelial cell line GES-1 were also studied. The 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（MTT） assay showed that TSI could dramatically reduce the viability rates of HTL-9 cells and MCF-7 cells without detectable cytotoxicity on GES-1 normal cells when the TSI concentration was lower than 100 μg/ml. With 100 μg/ml of TSI, HTL-9 cells were arrested in the G1 phase, and late-apoptosis was primarily induced, accompanied with partial early-apoptosis. TSI could induce primarily earlyapoptosis by arresting cells in the G1 phase of MCF-7 cells. For HTL-9 cells, Western-blot and reverse-transcriptase polymerase chain reaction（RT-PCR） analysis showed that TSI（100 μg/ml） can up-regulate the expression of Bax, Caspase-3, Caspase-8, and cytochrome c（Cyto-c）, indicating that TSI could induce cell apoptosis mainly through the mitochondrial pathway. In addition, the expression of p53 was up-regulated, while the expression of Survivin and nuclear factor κB（NF-κB） was down-regulated. All these results showed that TSI could induce apoptosis of HTL-9 cells by the regulation of multiple apoptosis-related genes.

关键词： Soybean isoflavones Ganoderma lucidum Transformation Antiproliferative activity Apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays

引用

Journal of zhejiang university-science B(Biomedicine & Biotechnology) 2018年第9期19卷 726-734页

作者： Qi-qi ZHENG Yuan-chao LU Zun-zhong YE Jian-feng PING Jian WU Yi-bin YING College of biosystems engineering and food science Zhejiang University Key Laboratory of on Site Processing Equipment for Agricultural Products Ministry of Agriculture Faculty of Agricultural and food science Zhejiang A&F University

p-Nitrophenylphosphate （PNPP） is usually employed as the substrate for enzyme-linked immunosorbent assays, p-Nitrophenol （PNP）, the product of PNPP, with the catalyst alkaline phosphatase （ALP）, will passivate an electrode, which limits applications in electrochemical analysis. A novel anti-passivation ink used in the preparation of a graphene/ionic liquid/chitosan composited （rGO/IL/Ghi） electrode is proposed to solve the problem. The anti-passivation electrode was fabricated by directly writing the graphene-ionic liquid-chitosan composite on a single-side conductive gold strip. A glassy carbon electrode, a screen-printed electrode, and a graphene-chitosan composite-modified screen-printed electrode were investigated for comparison. Scanning electron microscopy was used to characterize the surface structure of the four different electrodes and cyclic voltammetry was carried out to compare their performance. The results showed that the rGO/IL/Ghi electrode had the best performance according to its low peak potential and large peak current. Amperometdc responses of the different electrodes to PNP proved that only the rGO/IL/Chi electrode was capable of anti-passivation. The detection of cardiac troponin I was used as a test example for electrochemical immunoassay. Differential pulse voltammetry was performed to detect cardiac troponin I and obtain a calibration curve. The limit of detection was 0.05 ng/ml.

关键词： Electrochemical immunoassay Electrode ink Anti-passivation Ionic liquid p-Nitrophenol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of carbohydrate in Polygonatum sibiricum:fructo-oligosaccharide was a major component

引用

food Quality and Safety 2023年第3期7卷 439-446页

作者： Jiabei Xia Cenrong Zhang Kai Zhu Xingyu Mei Huan Cheng Shiguo Chen Xingqian Ye Jianle Chen Ningbo Innovation Center Zhejiang UniversityNingboChina College of biosystems engineering and food science National–Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agri-Food ProcessingFuli Institute of Food ScienceZhejiang Engineering Laboratory of Food Technology and EquipmentZhejiang UniversityHangzhouChina zhejiang university Zhongyuan Institute ZhengzhouChina school of Biological and Chemical engineering NingboTech UniversityNingboChina

Polygonatum sibiricum,famous for its physiological activities,is a popular Chinese food and a traditional Chinese herb containing many carbohydrates as the main ***,except for most reported polysaccharides,other detailed compositions of carbohydrates are still not *** verify the controversial existence of starch and investigate other components,especially oligosaccharides,we used iodine–potassium iodide colorimetric detection and enzymatic hydrolysis to determine ***,oligosaccharides were analyzed by thin-layer chromatography,gel permeation chromatography,high-performance anion exchange chromatography with pulsed amperometric detection,and hydrophilic interaction chromatography–electrospray tandem mass *** results showed that the rhizome of *** lacked starch,and fructooligosaccharides were the main component,accounting for approximately 28.95%.Oligosaccharides with degrees of polymerization above 10 were the most abundant *** study clearly illustrated the unknown carbohydrate components of the Polygonatum rhizome,promoting its functional value with new evidence.

关键词： Polygonatum sibiricum starch determination fructo-oligosaccharides HPAEC-PAD HILIC-ESI-MS/MS

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Concise Cascade Methods for Transgenic Rice Seed Discrimination using Spectral Phenotyping

引用

Plant Phenomics 2023年第3期5卷 564-580页

作者： Jinnuo Zhang Xuping Feng Jian Jin Hui Fang Department of Agricultural and Biological engineering Purdue UniversityWest LafayetteIN 47907USA College of biosystems engineering and food science Zhejiang UniversityHangzhouChina Huzhou Institute of zhejiang university HuzhouChina

Currently,the presence of genetically modified(GM)organisms in agro-food markets is strictly regulated by enacted legislation *** is essential to ensure the traceability of these transgenic products for food safety,consumer choice,environmental monitoring,market integrity,and scientific ***,detecting the existence of GM organisms involves a combination of complex,time-consuming,and labor-intensive techniques requiring high-level professional *** this paper,a concise and rapid pipeline method to identify transgenic rice seeds was proposed on the basis of spectral imaging technologies and the deep learning *** composition of metabolome across 3 rice seed lines containing the cry1Ab/cry1Ac gene was compared and studied,substantiating the intrinsic variability induced by these GM *** showed that near-infrared and terahertz spectra from different genotypes could reveal the regularity of GM metabolic *** established cascade deep learning model divided GM discrimination into 2 phases including variety classification and GM status *** could be found that terahertz absorption spectra contained more valuable features and achieved the highest accuracy of 97.04%for variety classification and 99.71%for GM status ***,a modified guided backpropagation algorithm was proposed to select the task-specific characteristic wavelengths for further reducing the redundancy of the original *** experimental validation of the cascade discriminant method in conjunction with spectroscopy confirmed its viability,simplicity,and effectiveness as a valuable tool for the detection of GM rice *** approach also demonstrated its great potential in distilling crucial features for expedited transgenic risk assessment.

关键词： fir simplicity consuming

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparation of PVA@PEI@BAC@CNC composite nanofibrous film with high efficiency filtration for PM2.5

引用

Frontiers of Materials science 2023年第4期17卷 171-182页

作者： Jingda Huang Yi Wang Yuxin Cai Yipeng Liang Shite Lin Enfu Wang Jinhuan Zhong Wenbiao Zhang Kuichuan Sheng College of biosystems engineering and food science Zhejiang UniversityHangzhou 310058China College of Chemistry and Materials engineering Zhejiang A&F UniversityHangzhou 311300China

It is still a challenge to prepare a water-and polymer-based electrospun air filter film with high efficiency filtration,low pressure drop,and good mechanical *** address this issue,polyvinyl alcohol(PVA)was employed as the main material,mixing polyethyleneimine(PEI),bamboo-based activated carbon(BAC)and cellulose nanocrystal(CNC)to construct the air filter film by electrostatic *** this system,the negatively charged BAC and CNC are fixed in the system through bonding with the positively charged PEI,showing a double adsorption *** is the mechanical filtration of the porous network structure constructed by PVA@PEI electrospun nanofibers,and the other is the electrostatic adsorption of PM2.5 on the surface of BAC and *** is significant that the resulting composite air filter displays a high filtration efficiency of 95.86%,a pressure drop of only 59 Pa,and good thermal ***,the introduced methyltrimethoxysilane(MTMS)endows it with good *** these excellent performances,this system can provide theoretical and technical references for the development of water-and polymerbased electrospun air filter film.

关键词： polyvinyl alcohol polyethyleneimine bamboo-based activated carbon cellulose nanocrystals electrospun nanofibrous film PM2.5

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Spectral feature characterization and nitrogen content prediction in soils with different particle sizes and moisture contents

引用

International Journal of Agricultural and Biological engineering 2008年第1期1卷 43-50页

作者： He Yong Shao Yongni Annia García Pereira Antihus Alexander Hernández Gómez Cen Haiyan College of biosystems engineering and food science Zhejiang University268 Kaixuan RoadHangzhou 310029China

The objective of this research is to analyze the influences of light source incidence angle,fiber height,moisture content,and particle size on loamy mixed soil ***(N)content calibration and cross-validation models at different moisture contents and particle sizes were obtained using partial least squares(PLS)*** data were collected using a *** height of 100 mm and light source angle at 45°were chosen to obtain the sharpest spectra without apparent scattering *** results show that moisture content and particle size strongly influenced the absorbance of the spectra,and a better N prediction model was obtained when the particle sizes were in the ranges of 0.5-1.0,1.0-2.0 and 2.0-5.0 mm,with the correlation coefficients(r)of 0.819,0.815 and 0.818,and standard errors of prediction(SEP)of 2.29,2.41 and 2.42 mg/kg,*** N prediction model was obtained when the soil was kept in its natural moisture content with r of 0.575 and SEP of 3.275 mg/kg,compared to the performance of dried soil samples with r of 0.815 and SEP of 2.425 mg/kg.

关键词： spectral feature prediction model soil moisture nitrogen content near infrared reflectance spectroscopy partial least squares

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In-silico Prediction of the Sweetness of Aspartame Analogues from QSPR Analysis

引用

Chinese Journal of Structural Chemistry 2018年第11期37卷 1689-1702页

作者：陈可先沈茜茜沈诗祎周夏陶李祖光陈忠秀 school of food science and Biotechnology Zhejiang Gongshang University College of Chemical engineering Zhejiang University of Technology

The extensive utilization of the low-energy dipeptide sweetener aspartame in foods leads to various studies on searching for new sweeteners in series. However, the real mechanistic cause of their sweetness power is still not completely known owing to their complex interactions with human sweet receptor, which may be different from that of other sweeteners to some extent. In this contribution, predictive quantitative structure-property relationship(QSPR) models have been developed for diverse aspartame analogues using Materials Studio 5.0 software. The optimal QSPR model(r2 = 0.913, r2 CV = 0.881 and r2 pred = 0.730) constructed by the genetic function approximation method has been validated by the tests of cross validation, randomization, external prediction and other statistical criteria, which shows that their sweetness power is mainly governed by their electrotopological-state indices(SssCH and SsNH), spatial descriptors(Shadow length: LX, ellipsoidal volume and Connolly surface occupied volume) and topological descriptors(Chi(3): cluster and Chi(0)(valence modified)), which partially supports both multipoint attachment theory proposed by Nofre and Tinti et al. and B-X theory proposed by Kier et al.. Present exploited results provide the key structural features for the sweetness power of aspartame analogues, supplement the mechanistic understanding of the sweet perception, and would be also helpful for the design of potent sweetener analogs prior to their synthesis.

关键词： structure-property relationship genetic function approximation sweetness potency dipeptides correlation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Research on mechanics properties of crop stalks:A review

引用

International Journal of Agricultural and Biological engineering 2016年第6期9卷 10-19页

作者： Du Dongdong Wang Jun Institute of Intelligent Agricultural Equipment College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058China

As one of the foundational research areas in agricultural engineering,the study of agricultural materials includes an important branch:mechanics properties of crop *** branch is now widely associated with design and improvement of agricultural equipment,breeding of new species,and development of reinforced *** article presents an overview of research on mechanics properties of crop stalks,including the mechanics measurement experiments,mechanics model and theory,microstructure and composition,and mechanics simulation *** current research relies mainly on the mechanics theory of engineering material,neglects the fact that crop stalk is a biological material with complex structure,anisotropy,viscoelasticity and *** addition,some simulation experiments are conducted in idealized conditions,beyond the ***,the paper proposes to create new material models for crop stalks,conduct more practical and effective simulation experiments,and promote cooperation in interdisciplinary studies for the development of mechanics properties of crop stalks in the future research.

关键词： crop stalk mechanics property measurement model theory microstructure composition mechanics simulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recent advances in flexible pressure/strain sensors using carbon nanotubes

引用

International Journal of Agricultural and Biological engineering 2022年第24期15卷 1-12页

作者： Chan Ma Ruiyun Zhou Lijuan Xie school of biosystems engineering and food science Zhejiang UniversityHangzhou 310058China Key Laboratory of Intelligent Equipment and Robotics for Agriculture of zhejiang Province Hangzhou 310058China

In the context of the information society,wearable devices show broad prospects in the fields of vital signs monitoring,plant health monitoring,artificial electronic *** an inseparable part of wearable devices,flexible pressure/strain sensors have attracted wide *** and active materials are core factors affecting sensing *** the past few years,the introduction of carbon materials,especially carbon nanotubes,has provided more possibilities for improving *** efforts have been devoted to researching pressure/strain sensors based on carbon nanotubes with excellent performance or unique ***,the process in recent years in novel flexible pressure/strain sensors based on carbon nanotubes is *** strategies are discussed because it suggests sensing performance with significant differences and gives additional features like biodegradability and corrosion resistance to ***,the scope of application has been extended to plant monitor,except for common human movement *** review can help the researchers compare the influence of different fabrication strategies on the sensing performance and provide references for fabricating sensors in different applications.

关键词： carbon nanotube flexible sensor strain sensor pressure sensor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning and characterization of an oxiranedicarboxylate hydrolase from Labrys sp. WH-1

引用

Journal of zhejiang university-science B(Biomedicine & Biotechnology) 2019年第12期20卷 995-1002页

作者： Wen-na BAO Zi-sheng LUO Shi-wang LIU Yuan-feng WU Pei-lian WEI Gong-nian XIAO Yong LIU College of biosystems engineering and food science Zhejiang UniversityHangzhou 310058China school of Biological and Chemical engineering Zhejiang University of Science and TechnologyHangzhou 310023China zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products Hangzhou 310023China

Objective:This study aimed to clone and characterize the oxiranedicarboxylate hydrolase(ORCH) from Labrys ***:Purification by column chromatography,characterization of enzymatic properties,gene cloning by protein terminal sequencing and polymerase chain reaction(PCR),and sequence analysis by secondary structure prediction and multiple sequence alignment were ***:The ORCH from Labrys ***-1 was purified 26-fold with a yield of 12.7%.It is a monomer with an isoelectric point(pl) of 8.57 and molecular mass of 30.2 *** was stable up to 55℃with temperature at which the activity of the enzyme decreased by 50% in 15 min(T5015) of 61℃and the half-life at 50℃(t1/2,50℃) of 51 min and was also stable from pH 4 to 10,with maximum activity at 55℃and pH *** is a metal-independent enzyme and strongly inhibited by Cu2+,Ag+,and anionic *** kinetic parameters(Km,kcat,and kcat/Km) were 18.7 mmol/L,222.3 s-1,and 11.9 mmol/(L s),*** ORCH gene,which contained an open reading frame(ORF) of 825 bp encoding 274 amino acid residues,was overexpressed in Escherichia coli and the enzyme activity was 33 times higher than that of the wild *** The catalytic efficiency and thermal stability of the ORCH from Labrys ***-1 were the best among the reported ORCHs,and it provides an alternative catalyst for preparation of L(+)-2,3-dihydrobutanedioic acid.

关键词： Oxiranedicarboxylate hydrolase(ORCH) L(+)-2,3-Dihydrobutanedioic acid Characterization Cloning

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：