Identification of carbohydrate in Polygonatum sibiricum:fructo-oligosaccharide was a major component

作     者：Jiabei Xia Cenrong Zhang Kai Zhu Xingyu Mei Huan Cheng Shiguo Chen Xingqian Ye Jianle Chen Jiabei Xia;Cenrong Zhang;Kai Zhu;Xingyu Mei;Huan Cheng;Shiguo Chen;Xingqian Ye;Jianle Chen

作者机构：Ningbo Innovation CenterZhejiang UniversityNingboChina College of Biosystems Engineering and Food ScienceNational–Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agri-Food ProcessingFuli Institute of Food ScienceZhejiang Engineering Laboratory of Food Technology and EquipmentZhejiang UniversityHangzhouChina Zhejiang University Zhongyuan InstituteZhengzhouChina School of Biological and Chemical EngineeringNingboTech UniversityNingboChina 

出 版 物：《Food Quality and Safety》 (食品品质与安全研究（英文版）)

年 卷 期：2023年第7卷第3期

页      面：439-446页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：This work was financially supported by the grants from Ningbo Municipal Bureau of Science and Technology(2022Z174) The Major Agricultural Technology Collaborative Promotion Plan of Zhejiang Province(2022XTTGZYC03) The Key Research and Development Program of Zhejiang Province(2021C02001),China 

主　　题：Polygonatum sibiricum starch determination fructo-oligosaccharides HPAEC-PAD HILIC-ESI-MS/MS 

摘      要：Polygonatum sibiricum,famous for its physiological activities,is a popular Chinese food and a traditional Chinese herb containing many carbohydrates as the main ***,except for most reported polysaccharides,other detailed compositions of carbohydrates are still not *** verify the controversial existence of starch and investigate other components,especially oligosaccharides,we used iodine–potassium iodide colorimetric detection and enzymatic hydrolysis to determine ***,oligosaccharides were analyzed by thin-layer chromatography,gel permeation chromatography,high-performance anion exchange chromatography with pulsed amperometric detection,and hydrophilic interaction chromatography–electrospray tandem mass *** results showed that the rhizome of *** lacked starch,and fructooligosaccharides were the main component,accounting for approximately 28.95%.Oligosaccharides with degrees of polymerization above 10 were the most abundant *** study clearly illustrated the unknown carbohydrate components of the Polygonatum rhizome,promoting its functional value with new evidence.