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Biomedical and Environmental Sciences 2019年第10期32卷 755-768页

作者： ZHANG Jia Yan XIAO Xiang DONG Ying ZHOU Xing Hua school of food and biological engineering Jiangsu University

Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese *** Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the *** LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling *** These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.

关键词： Fermented barley extract Lactobacillus plantarum dy-1 Anti-obesity Lipid metabolism miRNAs Microarray

Fermented Barley Extracts with Lactobacillus plantarum dy-1 Rich in Vanillic Acid Modulate Glucose Consumption in Human HepG2 Cells

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Biomedical and Environmental Sciences 2018年第9期31卷 667-676页

作者： ZHANG Jia Yan XIAO Xiang DONG Ying ZHOU Xing Hua school of food and biological engineering Jiangsu University

Objective To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1（LFBE） for modulating glucose consumption in HepG2 cells via miR-212 regulation. Methods Hepatocellular carcinoma（HepG2） cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. Results Treatment with LFBE rich in vanillic acid（VA） increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9（DUSP9）, a direct target of miR-212, at both protein and mR NA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression. Conclusion The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.

关键词： Fermented barley extract Vanillic acid Glucose consumption HepG2 Dual-specificity phosphatase-9

Supplementation of Fermented Barley Extracts with Lactobacillus Plantarum dy-1 Inhibits Obesity via a UCP1-dependent Mechanism

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Biomedical and Environmental Sciences 2019年第8期32卷 578-591页

作者： XIAO Xiang BAI Juan LI Ming Song ZHANG Jia Yan SUN Xin Juan DONG Ying school of food and biological engineering Jiangsu University

Objective We aimed to explore how fermented barley extracts with Lactobacillus plantarum dy-1(LFBE) affected the browning in adipocytes and obese *** In vitro, 3T3-L1 cells were induced by LFBE, raw barley extraction(RBE) and polyphenol compounds(PC) from LFBE to evaluate the adipocyte *** vivo, obese SD rats induced by high fat diet(HFD) were randomly divided into three groups treated with oral gavage:(a) normal control diet with distilled water,(b) HFD with distilled water,(c) HFD with 800 mg LFBE/kg body weight(bw).Results In vitro, LFBE and the PC in the extraction significantly inhibited adipogenesis and potentiated browning of 3T3-L1 preadipocytes, rather than *** vivo, we observed remarkable decreases in the body weight, serum lipid levels, white adipose tissue(WAT) weights and cell sizes of brown adipose tissues(BAT) in the LFBE group after 10 *** group could gain more mass of interscapular BAT(IBAT) and promote the dehydrogenase activity in the *** LFBE may potentiate process of the IBAT thermogenesis and epididymis adipose tissue(EAT) browning via activating the uncoupling protein 1(UCP1)-dependent mechanism to suppress the *** These results demonstrated that LFBE decreased obesity partly by increasing the BAT mass and the energy expenditure by activating BAT thermogenesis and WAT browning in a UCP1-dependent mechanism.

关键词： Fermented barley Polyphenol compounds UCP1 Thermogenesis Browning

Impact of three different processing methods on the digestibility and allergenicity of Chinese mitten crab(Eriocheir sinensis)tropomyosin

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food Science and Human Wellness 2023年第3期12卷 903-911页

作者： Yufeng Lu Huafeng Cheng Shaotong Jiang Lin Lin Jianfeng Lu engineering Research Center of Bio-process MOESchool of Food and Biological EngineeringHefei University of TechnologyHefei 230000China Key Laboratory for Agricultural Products processing of Anhui Province School of Food and Biological EngineeringHefei University of TechnologyHefei 230000China

The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently ***,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were *** dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of *** contrast,ultrasound and HTP treatments facilitated the degradation of ***,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or *** the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing *** finding provides new insights into treatments for crab allergies.

关键词： Chinese mitten crab Tropomyosin processing methods Immunoreactivity

Evaluation of the binding affinity and antioxidant activity of phlorizin to pepsin and trypsin

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food Science and Human Wellness 2024年第1期13卷 392-400页

作者： Jing Zhang Lan Tang Xia Hu Zhen Zeng Wen Wu Fang Geng Hui Li Di Wu Meat processing Key Laboratory of Sichuan Province School of Food and Biological EngineeringChengdu UniversityChengdu 610106China school of Chemical engineering Sichuan UniversityChengdu 610065China

Phlorizin(PHL)is a natural compound with strong antioxidant properties mainly found in *** this paper,the interaction mechanism of PHL with pepsin and trypsin was comparatively evaluated by computer simulation,fluorescence spectra,circular dichroism(CD),and Fourier transform infrared(FT-IR)spectra at a molecular *** spectra showed that PHL quenches the pepsin/trypsin by static *** parameters indicated that PHL binds to pepsin mainly through hydrogen bonds and van der Waals forces,and that of trypsin was electrostatic *** ground state complexes PHL and protease have a moderate affinity of 105 L/mol PHL binds more strongly to trypsin than to *** and FT-IR spectra results showed that pepsin/trypsin decreased theβ-sheet content and slightly changed its secondary structure upon *** experimental results are mutually verified with the predicted computer-aid simulation *** PHL and trypsin binding,the antioxidant capacity of PHL was ***,the antioxidant capacity of PHL was decreased after binding to *** work elucidates the binding of PHL binding mechanisms to pepsin/trypsin and provides useful information for the digestion of PHL to improve the application of PHL in food processing.

关键词： Interaction Phlorizin Pepsin Trypsin Antioxidation

Unlocking the stable interface in aqueous zinc-ion battery with multifunctional xylose-based electrolyte additives

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Journal of Energy Chemistry 2025年第1期 770-778页

作者： Xiaoqin Li Jian Xiang Lu Qiu Xiaohan Chen Yinkun Zhao Yujue Wang Qu Yue Taotao Gao Wenlong Liu Dan Xiao Zhaoyu Jin Panpan Li Institute for Advanced Study Chengdu University school of Mechanical engineering Chengdu University College of food and biological engineering Chengdu University Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China College of Materials Science and engineering Sichuan University

The growth of dendrites and the side reactions occurring at the Zn anode pose significant challenges to the commercialization of aqueous Zn-ion batteries（AZIBs）. These challenges arise from the inherent conflict between mass transfer and electrochemical kinetics. In this study, we propose the use of a multifunctional electrolyte additive based on the xylose（Xylo） molecule to address these issues by modulating the solvation structure and electrode/electrolyte interface, thereby stabilizing the Zn anode. The introduction of the additive alters the solvation structure, creating steric hindrance that impedes charge transfer and then reduces electrochemical kinetics. Furthermore, in-situ analyses demonstrate that the reconstructed electrode/electrolyte interface facilitates stable and rapid Zn2+ion migration and suppresses corrosion and hydrogen evolution reactions. As a result, symmetric cells incorporating the Xylo additive exhibit significantly enhanced reversibility during the Zn plating/stripping process, with an impressively long lifespan of up to 1986 h, compared to cells using pure ZnSO4electrolyte. When combined with a polyaniline cathode, the full cells demonstrate improved capacity and long-term cyclic stability. This work offers an effective direction for improving the stability of Zn anode via electrolyte design, as well as highperformance AZIBs.

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Rapid quantification of acid value in frying oil using iron tetraphenylporphyrin fluorescent sensor coupled with density functional theory and multivariate analysis

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food Quality and Safety 2022年第4期6卷 534-544页

作者： Haiyang Gu Yining Dong Riqin Lv Xingyi Huang Quansheng Chen Department of biological and Chemical engineering Yangzhou Polytechnic CollegeYangzhouChina school of Bio and food engineering Chuzhou UniversityChuzhouChina school of food and biological engineering Jiangsu UniversityZhenjiangChina

A metalloporphyrin-based fluorescent sensor was developed to determine the acid value in frying *** electronic and structural performances of iron tetraphenylporphyrin(FeTPP)were theoretically investigated using time-dependent density functional theory and density functional theory at the B3LYP/LANL2DZ *** quantified FeTPP-based fluorescent sensor results revealed its excellent performance in discriminating different *** the present work,the acid value of palm olein was determined after every single frying cycle.A total of 10 frying cycles were conducted each day for 10 consecutive *** FeTPP-based fluorescent sensor was used to quantify the acid value,and the results were compared with the chemical data obtained by conventional titration *** synchronous fluorescence spectrum for each sample was *** factor analysis was used to decompose the three-dimensional spectrum ***,the support vector regression(SVR),partial least squares,and back-propagation artificial neural network methods were applied to build the regression *** the comparison of the constructed models,the SVR models exhibited the highest correlation coefficients among all models,with 0.9748 and 0.9276 for the training and test sets,*** findings suggested the potential of FeTPP-based fluorescent sensor in rapid monitoring of frying oil quality and perhaps also in other foods with higher oil contents.

关键词： Synchronous fluorescence spectrum fluorescent sensor oil quality density functional theory parallel factor analysis

Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells

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food Science and Human Wellness 2024年第3期13卷 1503-1512页

作者： Wen Nie Feiran Xu Kai Zhou Jieying Deng Ying Wang Baocai Xu engineering Research Center of Bio-process Ministry of EducationSchool of Food and Biological EngineeringHefei University of TechnologyHefei 230601China school of biological and food engineering Chuzhou UniversityChuzhou 239001China

The study evaluated the stability of an oligopeptide(Lys-Arg-Gln-Lys-Tyr-Asp,KRQKYD)and its transport mechanism by simulating gastrointestinal digestion and a model of human intestinal Caco-2 monolayer cells in *** this study,the effects of environmental factors(temperature,pH and NaCl concentration)and simulated gastrointestinal digestion on the stability of KRQKYD were evaluated by indicators of the levels of alanine transaminase(ALT),aspartate transaminase(AST)and malondialdehyde(MDA)in an alcoholinduced hepatocyte injury *** results showed that KRQKYD still maintained satisfactory hepatocyteprotective activity after treatment with different temperatures(20-80℃),pH(3.0-9.0),NaCl concentration(1%-7%)and simulated gastrointestinal digestion,which indicated that KRQKYD showed good stability to environmental factors and simulated gastrointestinal ***,the intact KRQKYD could be absorbed in a model of Caco-2 monolayer cells with a P_(app)value of(9.70±0.53)×10^(-7)cm/*** with an energy inhibitor(sodium azide),a competitive peptide transporter inhibitor(Gly-Pro)and a transcytosis inhibitor wortmannin did not decrease the level of transepithelial KRQKYD transport,indicating that the transport mechanism of KRQKYD was not associated with energy dependent,vector mediated and *** tight junction disruptor cytochalasin D significantly increased the level of transepithelial KRQKYD transport(P<0.05),suggesting that intact KRQKYD was absorbed by paracellular transport.

关键词： Jinhua ham KRQKYD(Lys-Arg-Gln-Lys-Tyr-Asp) Environmental stability Gastrointestinal digestive Transport mechanism

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Cell membrane-coated nanoparticles for immunotherapy

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Chinese Chemical Letters 2022年第4期33卷 1673-1680页

作者： Hang Liu Zhaohua Miao Zhengbao Zha school of food and biological engineering Hefei University of TechnologyHefei 230009China

Nanoparticle-based disease detection, prevention and therapies have gained increased interests in biomedical applications, owing to their significant advantages in therapeutic efficacy and safety. Nonetheless, suffering from the challenges including fast recognition and clearance of foreign nanoparticles by innate immune system before arriving at diseased regions, clinical applications of nanoparticles are usually intercepted. Among various strategies for reducing non-specific phagocytosis and enhancing diseasetargeting efficiency of nanoparticles, membrane coating nanotechnology exhibits great potential in the disease diagnosis and therapeutics due to both the structural and functional preservation of membrane proteins from source cells. Benefiting the inherited immune-regulation capacities, this review mainly summarized the latest development of such biomimetic nanoparticles for immunotherapy in treating immune-related diseases including microbial infections, inflammation, tumor and autoimmune diseases.

关键词： Biomimetic nanoparticles Immunotherapy Nanovaccine Cell membrane

Emerging markers for antimicrobial resistance monitoring

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Chinese Chemical Letters 2023年第10期34卷 55-66页

作者： Zineng Yi Xiaona Xu Xiaohan Meng Congyu Liu Qianpeng Zhou Deyan Gong Zhengbao Zha school of food and biological engineering Hefei University of TechnologyHefei 230009China

The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next *** of the main methods for addressing AMR is the effective screening for antimicrobial insensitivity in clinical and environmental *** clinical laboratory procedures use traditional culturebased antibiotic susceptibility testing(AST)methods,which can take up to 24 h to identify which drug is suitable for the infection ***,it is vital to develop novel strategies that offer quick,simple,affordable,reliable,sensitive and accurate AMR *** for AMR markers detection could possess the essential qualities for quickly identifying resistant microorganisms and could give vital data for the selection of antibacterial drugs *** review offers a summary of the innovative application of these AMR markers detection strategies focusing on healthcare and environmental surveillance for the AMR genotypic or phenotypic assessment.

关键词： Antimicrobial resistance markers Genotypic determination Enzymes detection Antibody labeling Protein labeling