Fermented Barley Extracts with Lactobacillus plantarum dy-1 Rich in Vanillic Acid Modulate Glucose Consumption in Human HepG2 Cells

作     者：ZHANG Jia Yan XIAO Xiang DONG Ying ZHOU Xing Hua 

作者机构：School of Food and Biological Engineering Jiangsu University 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2018年第31卷第9期

页      面：667-676页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100403[医学-营养与食品卫生学] 10[医学] 

基　　金：supported by the priority academic program development of Jiangsu Higher education institutions and the National Natural Science Foundation of China [31501569 31371760] 

主　　题：Fermented barley extract Vanillic acid Glucose consumption HepG2 Dual-specificity phosphatase-9 

摘      要：Objective To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1（LFBE） for modulating glucose consumption in HepG2 cells via miR-212 regulation. Methods Hepatocellular carcinoma（HepG2） cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. Results Treatment with LFBE rich in vanillic acid（VA） increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9（DUSP9）, a direct target of miR-212, at both protein and mR NA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression. Conclusion The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.