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Chinese Chemical Letters 2024年第4期35卷 426-430页

作者： Tiantian Li Ruochen Jin Bin Wu Dongming Lan Yunjian Ma Yonghua Wang school of Food Science and Engineering South China University of TechnologyGuangzhou 510640China school of bioscience and bioengineering South China University of TechnologyGuangzhou 510006China Guangdong Youmei Institute of Intelligent Bio-manufacturing Co. Ltd.Foshan 528200China

Unspecific peroxygenases(UPOs, EC 1.***.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H_(2)O_(2) as an oxygen donor without cofactors. However, the enhancement of the H_(2)O_(2) tolerance of UPOs is always challenging. In this study, the A161C mutant of r Dca UPO,which originates from Daldinia caldariorum, was found to be highly H_(2)O_(2)-resistant. Compared with the wild type, the mutant r Dca UPO-A161C showed a 10-h prolonged half-life and a 64% improved enzyme activity when incubated in 10 mmol/L H_(2)O_(2). The crystal structure analysis at 1.47 A showed that r Dca UPOA161C exhibited 10 α-helixes(cyan) and a series of ordered rings, forming a single asymmetric spherical structure. The two conserved domains near heme formed an active site with the catalytic PCP and EHD regions(Glu86, His87, Asp88 residues). The H_(2)O_(2) tolerance of r Dca UPO-A161C was preliminarily explored by comparing its structure with the wild type. Notably, r Dca UPO-A161C showed significantly higher catalytic efficiency than the wild type for the production of hydroxyl fatty acids. This study is anticipated to provide an insight into the structure-function relationship and expand potential applications of UPOs.

关键词： Unspecific peroxygenase Molecular modification Hydrogen peroxide stability Crystal structure Hydroxyl fatty acids

The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes

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Virologica Sinica 2024年第2期39卷 228-234页

作者： Ru-Yi Chen Teng Zhao Jing-Jing Guo Feng Zhu Na-Na Zhang Xiao-Feng Li Hai-Tao Liu Fei Wang Yong-Qiang Deng Cheng-Feng Qin College of bioscience and bioengineering Jiangxi Agricultural UniversityNanchang330045China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMSBeijing100071China school of Life Sciences Southwest Forestry UniversityKunming650224China

Guaico Culex virus(GCXV)is a newly identified segmented Jingmenvirus from Culex *** in Central and South *** genome of GCXV is composed of four or five single-stranded positive RNA ***,the infection kinetics and transmission capability of GCXV in mosquitoes remain *** this study,we used reverse genetics to rescue two GCXVs(4S and 5S)that contained four and five RNA segments,respectively,in C6/36 *** in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics,protein expression and viral ***,GCXV RNAs were detected in the bodies,salivary glands,midguts and ovaries of Culex quinquefasciatus at 4–10 days after oral *** addition,two GCXVs can colonize *** eggs,resulting in positive rates of 15%–35%for the second gonotrophic *** conclusion,our results demonstrated that GCXVs with four or five RNA segments can be detected in *** eggs during the first and second gonotrophic cycles after oral infection.

关键词： Guaico Culex virus(GCXV) Jingmenvirus Culex quinquefasciatus Vertical transmission

On-chip physiological mimicry of neurovascular unit: challenges and perspectives

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Neural Regeneration Research 2024年第3期19卷 499-500页

作者： Song Ih Ahn YongTae Kim school of Mechanical Engineering Pusan National UniversityBusanSouth Korea George W.Woodruff school of Mechanical Engineering Georgia Institute of TechnologyAtlantaGAUSA Parker H.Petit Institute for bioengineering and bioscience Georgia Institute of TechnologyAtlantaGAUSA Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of TechnologyAtlantaGAUSA Institute for Electronics and Nanotechnology Georgia Institute of TechnologyAtlantaGAUSA

Neurological disorders including neurodegenerative diseases,brain tumors,and stroke are the second leading cause of death and the greatest cause of disability ***,it remains challenging to achieve effective drug delivery to the central nervous system for treatments of neurological diseases due to the blood-brain barrier(BBB).The function of the BBB is regulated by the physiological interactions between various types of cells in the neurovascular unit(NVU).In the NVU,the brain vasculature of the BBB is surrounded by brain pericytes,brain astrocytes,neurons,and microglia(Figure 1).Moreover,the NVU at the levels of arteries and veins includes contractile smooth muscle cells(Schaeffer and Iadecola,2021).

关键词： diseases death arteries

Optimization of Fermentation Media for Enhancing Nitrite-oxidizing Activity by Artificial Neural Network Coupling Genetic Algorithm

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Chinese Journal of Chemical Engineering 2012年第5期20卷 950-957页

作者：罗剑飞林炜铁蔡小龙李敬源 school of bioscience and bioengineering South China University of Technology

Two artificial intelligence techniques, artificial neural network and genetic algorithm, were applied to optimize the fermentation medium for improving the nitrite oxidization rate of nitrite oxidizing bacteria. Experiments were conducted with the composition of medium components obtained by genetic algorithm, and the experimental data were used to build a BP (back propagation) neural network model. The concentrations of six medium components were used as input vectors, and the nitrite oxidization rate was used as output vector of the model. The BP neural network model was used as the objective function of genetic algorithm to find the optimum medium composition for the maximum nitrite oxidization rate. The maximum nitrite oxidization rate was 0.952 g 2 NO-2-N·(g MLSS)-1·d-1 , obtained at the genetic algorithm optimized concentration of medium components (g·L-1 ): NaCl 0.58, MgSO 4 ·7H 2 O 0.14, FeSO 4 ·7H 2 O 0.141, KH 2 PO 4 0.8485, NaNO 2 2.52, and NaHCO 3 3.613. Validation experiments suggest that the experimental results are consistent with the best result predicted by the model. A scale-up experiment shows that the nitrite degraded completely after 34 h when cultured in the optimum medium, which is 10 h less than that cultured in the initial medium.

关键词： BP neural network genetic algorithm optimization nitrite oxidization rate nitrite-oxidizing bacteria

Syntheses,Crystal Structures and Theoretical Studies of Three Novel Salts of Vortioxetine and the Investigation of Their Solubility

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Chinese Journal of Structural Chemistry 2016年第11期35卷 1645-1654页

作者：张硕张宪瑞何赛飞李晶张雷 school of bioscience and bioengineering South China University of Technology

Three new vortioxexine （VOT） salts with salicylic acid （SA）, 5-fluorouracil （FU） and （p-nitrophenyl）-acetic acid （PA） have been synthesized and characterized in detail. In VOT-SA（1：1）, the protonated VOT and deprotonated salicylic anion form a tetrameric unit with the R4 4（12） synthon via N（1）–H（1）…O（1）^a （a： x–1, y, z） and N（1）–H（2）…O（2）^b （b： –x, –y, –z＋1） hydrogen bonds. However, in VOT–FU（1：2）, the protonated VOT and deprotonated 5-fluorouracil anion feature an R4 4（16） ring motif via N（1）–H（1）…O（2）^c （c： –x＋1, –y＋1, –z＋1） and N（1）– H（2）…O（1）^d （d： x, y–1, z） hydrogen bonds. The neutral FU extends the R4 4（16） ring to form a two-dimensional structure through R2 2（8） N–H…O and N（6）–H（4）…N（4）……e （e： –x＋1, y–1/2, –z＋1/2） hydrogen bonds. In VOT-PA（1：1）, PA molecules are linked through N（1）–H（1）…O（1）f （f： –x＋1, y＋1/2, –z＋1） and N（1）–H（2）…O（2）, which forms a right-handed helical structure. Furthermore, components of the crystalline phase have also been investigated in terms of their corresponding Hirshfeld surface, and the salification improves the equilibrium solubilities and dissolution rates of VOT in three salts at 25 ℃ in water.

关键词： vortioxetine crystal structure Hirshfeld surface equilibrium solubility powder dissolution

Dinuclear Nickel(Ⅱ) Complex with a Rare Ni(μ_2-O)_3Ni Core: Crystal Structure and Magnetic Studies

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Chinese Journal of Structural Chemistry 2012年第2期31卷 199-204页

作者：张雷黄志平凌云李晶 school of bioscience and bioengineering South China University of Technology

The first triple μ2-O-bridged dinuclear Ni（II） complex, [Ni2（Flu）3]（ClO4）·（H2O）（1, HFlu = 2-（2′,4′-difluorophenyl）-1,3-bis（1,2,4-triazol-1-yl）-propan-2-ol）, has been synthesized and structurally and magnetically characterized. Complex 1 crystallizes in monoclinic, space group P21/n with a = 12.7143（3）, b = 21.3392（6）, c = 17.6497（5）, β = 106.142（2）o, V = 4599.8（2）3, Z = 4, μ（MoKα） = 0.975 mm-1, Dc = 1.662 g/cm3, GOF = 0.83, the final R （I 〉 2σ（I）） = 0.0503 and wR （all data） = 0.1288. In 1, three Flu anions encapsulate two nickel（II） ions bridged by three deprotonated μ2-O groups. The central alkoxo and terminal triazolyl group chelate one nickel（II） in a six-membered boat configuration. Variable temperature magnetic susceptibilities show an intramolecular anti-ferromagnetic interaction between the Ni（II） ions with J = -26.7（3） cm-1.

关键词： nickel complex fluconazole crystal structure magnetic property

Stimulated emission-depletion-based point-scanning structured illumination microscopy

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Chinese Optics Letters 2024年第3期22卷 95-100页

作者：汪磊王美婷王璐玮郑晓敏陈嘉杰吴文帅严伟于斌屈军乐高志邵永红 Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060China Key Laboratory of Opto-electronic Information Science and Technology of Jiangxi Province Nanchang Hangkong UniversityNanchang 330063China College of Physics and Optoelectronics Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen UniversityShenzhen 518060China Department of bioengineering and COMSET Clemson UniversityClemson SC 29634US

Wide-field linear structured illumination microscopy(LSIM)extends resolution beyond the diffraction limit by moving unresolvable high-frequency information into the passband of the microscopy in the form of moiré***,due to the diffraction limit,the spatial frequency of the structured illumination pattern cannot be larger than the microscopy cutoff frequency,which results in a twofold resolution improvement over wide-field *** Letter presents a novel approach in point-scanning LSIM,aimed at achieving higher-resolution improvement by combining stimulated emission depletion(STED)with point-scanning structured illumination microscopy(ps SIM)(STED-ps SIM).The according structured illumination pattern whose frequency exceeds the microscopy cutoff frequency is produced by scanning the focus of the sinusoidally modulated excitation beam of STED *** experimental results showed a 1.58-fold resolution improvement over conventional STED microscopy with the same depletion laser power.

关键词： stimulated emission depletion structured illumination microscopy superresolution microscopy

Functional localization of the cortical motor area in the brain Electrocorticogram analysis

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Neural Regeneration Research 2010年第19期5卷 1480-1485页

作者： Tao Jiang Xiaoming Wu Binggang Ye Sijuan Huang school of bioscience & bioengineering South China University of Technology Guangzhou 510006 Guangdong Province China

The method for rapidly, precisely and non-invasively localizing functional regions of the brain is a problem in neuromedicine research. Cortical electrostimulation is the optimal localization method during brain surgery, with a degree of accuracy of approximately 5 mm. However, electrostimulation can damage the cerebral cortex, trigger epilepsy, and extend the operation time. Studies are required to determine whether cortical motor regions can be localized by wavelet analysis from electrocorticograms. In this study, based on wavelet analysis of electrocorticograms, a selection of algorithms for classification of the mu rhythm in the motor regions utilizing experimental data was verified. Results demonstrated that a characteristic quantity of energy ratio in the reconstructed signal was filtered in the d6 （7.81-15.62 Hz） band prior to and following motion events. A characteristic threshold was considered to be 40%. The accuracy of localization detection was 93%. The degree of accuracy was less than 5 mm. The present study avoided the problems of cerebral cortex injury and epilepsy onset, with an operation time of 60 seconds. Therefore, wavelet analysis on electrocorticogram is feasible for localizing cortical motor regions. Furthermore, this localization technique is accurate, safe and rapid.

关键词： functional localization electrocorticogram mu rhythm event-related desynchronization wavelet analysis neural regeneration

Terahertz Irradiation Improves Cognitive Impairments and Attenuates Alzheimer’s Neuropathology in the APPSWE/PS1DE9 Mouse:A Novel Therapeutic Intervention for Alzheimer’s Disease

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Neuroscience Bulletin 2024年第7期40卷 857-871页

作者： Jun Zhang Yixin Chen Yarui Zhao Panpan Wang Hongbin Ding Cong Liu Junhong Lyu Weidong Le Liaoning Provincial Key Laboratory for Research on the Pathogenic Mechanisms of Neurological Diseases the First Affiliated HospitalDalian Medical UniversityDalian 116011China school of Physics Dalian University of TechnologyDalian 116024China interdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 201210China Advanced Research Institute Chinese Academy of SciencesShanghai 201203China Department of Neurology and Institute of Neurology Sichuan Academy of Medical Sciences‑Sichuan Provincial HospitalMedical SchoolUniversity of Electronic Science and Technology of ChinaChengdu 610072China

Alzheimer's disease(AD)is a progressive neurodegenerative disease characterized by the deposition of amyloid-β(Aβ),neurofibrillary tangles,neuroinflammation,and neurodegeneration in the *** recent years,considering the unsatisfied benefits of pharmacological therapies,non-pharmacological therapy has become a research hotspot for AD ***(THz)waves with a range between microwave and infrared regions in the electromagnetic spectrum and high permeability to a wide range of materials have great potential in the bioengineering ***,its biological impacts on the central nervous system,under either physiological or pathological conditions,are poorly *** this study,we first measured the 0.14 THz waves penetration across the skull of a C57BL/6 mouse and found the percentage of THz penetration to be~70%,guaranteeing that THz waves can reach the relevant brain *** then exposed the APPSWE/PS1DE9 mouse model of AD to repeated low-frequency THz waves on the *** demonstrated that THz waves treatment significantly improved the cognitive impairment and alleviated AD neuropathology including Aβdeposition and tau hyperphosphorylation in the AD ***,THz waves treatment effectively attenuated mitochondrial impairment,neuroinflammation,and neuronal loss in the AD mouse *** findings reveal previously unappreciated beneficial effects of THz waves treatment in AD and suggest that THz waves may have the potential to be used as a novel therapeutic intervention for this devastating disease.

关键词： Alzheimer's disease Cognitive impairment Senile plaque Tau protein Terahertz

Three-Dimensional Reconstruction of Icosahedral Virus by Symmetry-Adapted Functions

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Chinese Physics Letters 2007年第6期24卷 1767-1770页

作者：刘红荣杨奇斌程凌鹏曾松军蔡灿英 Institute of Modern Physics Xiangtan University Xiangtan 411105 school of bioscience and bioengineering South China University of Technology Guangzhou 510640

A three-dimensional （3D） reconstruction of icosahedral virus is carried out by the icosahedral symmetry-adapted function （ISAF） method in spherical coordinates. In order to reduce the influence of noise, it is better to use the basis functions that have identical symmetry with the object reconstructed. It is verified that the ISAF method has stronger ability to reduce the influence of noise to grain the resolution better than that of the conventional method by the simulation of 3D reconstruction.

关键词： ELECTRON CRYOMICROSCOPY CRYOELECTRON MICROSCOPY SUBNANOMETER RESOLUTION PARTICLES MICROGRAPHS PRINCIPLES LINE