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Endomembrane-biased dimerization of ABCG16 and ABCG25 transporters determines their substrate selectivity in ABA-regulated plant growth and stress responses

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Molecular plant 2024年第3期17卷 478-495页

作者： Yeling Zhou Yuzhu Wang Dong Zhang Jiansheng Liang institute of plant and food science Department of BiologySchool of Life SciencesSouthern University of Science and Technology(SUSTech)Shenzhen 518055China Key Laboratory of Molecular Design for plant Cell Factory of Guangdong Higher Education institutes Southern University of Science and Technology(SUSTech)Shenzhen 518055China jiangsu Key Laboratory of Crop Genetics and Physiology/Co-lnnovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhou 225009China

ATP-binding cassette(ABC)transporters are integral membrane proteins that have evolved diverse func-tions fulfilled via the transport of various *** Arabidopsis,the G subfamily of ABC proteins is particularly abundant and participates in multiple signaling pathways during plant development and stress *** this study,we revealed that two Arabidopsis ABCG transporters,ABCG16 and ABCG25,engage in ABA-mediated stress responses and early plant growth through endomembrane-specific dimerization-coupled transport of ABA and ABA-glucosyl ester(ABA-GE),*** first revealed that ABCG16 contributes to osmotic stress tolerance via ABA *** specifically,ABCG16 induces cellular ABA efflux in both yeast and plant *** FRET analysis,we showed that ABCG16 forms oblig-atory homodimers for ABA export activity and that the plasma membrane-resident ABCG16 homodimers specifically respond to ABA,undergoing notable conformational ***,we demonstrated that ABCG16 heterodimerizes with ABCG25 at the endoplasmic reticulum(ER)membrane and facilitates the ER entry of ABA-GE in both Arabidopsis and tobacco *** specific responsiveness of the ABCG16-ABCG25 heterodimer to ABA-GE and the superior growth of their double mutant support an inhib-itory role of these twoABCGs in early seedling establishment via regulation of ABA-GE translocation across the ER *** endomembrane-specific analysis of the FRET signals derived from the homo-or heterodimerized ABcG complexes allowed us to link endomembrane-biased dimerization to the transloca-tion of distinct substrates by ABcG transporters,providing a prototypic framework for understanding the omnipotence of ABcG transporters in plant development and stress responses.

关键词： abscisic acid ABCG transporter dimerization endomembrane substrate selectivity

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Making small molecules in plants:A chassis for synthetic biology-based production of plant natural products

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Journal of Integrative plant Biology 2023年第2期65卷 417-443页

作者： Xinyu Liu Peijun Zhang Qiao Zhao Ancheng C.Huang Key Laboratory of Molecular Design for plant Cell Factory of Guangdong Higher Education institutes Department of BiologySchool of Life SciencesSUSTech-PKU Institute of Plant and Food ScienceSouthern University of Science and TechnologyShenzhen 518055China Shenzhen institutes of Advanced Technology(SIAT) the Chinese Academy of SciencesShenzhen 518055China

plant natural products have been extensively exploited in food,medicine,flavor,cosmetic,renewable fuel,and other industrial *** biology has recently emerged as a promising means for the cost-effective and sustainable production of natural *** with engineering microbes for the production of plant natural products,the potential of plants as chassis for producing these compounds is underestimated,largely due to challenges encountered in engineering *** in plant engineering is instrumental for enabling the effective and efficient production of valuable phytochemicals in plants,and also paves the way for a more sustainable future *** this manuscript,we briefly recap the biosynthesis of plant natural products,focusing primarily on industrially important terpenoids,alkaloids,and *** further summarize the plant hosts and strategies that have been used to engineer the production of natural *** challenges and opportunities of using plant synthetic biology to achieve rapid and scalable production of high-value plant natural products are also discussed.

关键词： biosynthesis natural products plant engineering synthetic biology

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Interplay of phytohormones and epigenetic regulation:A recipe for plant development and plasticity

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Journal of Integrative plant Biology 2023年第2期65卷 381-398页

作者： Kai Jiang Hongwei Guo Jixian Zhai institute of plant and food science Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education InstitutesDepartment of BiologySchool of Life SciencesSouthern University of Science and Technology(SUSTech)Shenzhen 518055China

Both phytohormone signaling and epigenetic mechanisms have long been known to play crucial roles in plant development and plasticity in response to ambient ***,diverse signaling pathways mediated by phytohormones and epigenetic processes integrate multiple upstream signals to regulate various plant *** evidence indicates that phytohormones and epigenetic processes interact at multiple *** this review,we summarize the current knowledge of the interplay between phytohormones and epigenetic processes from the perspective of phytohormone *** also review chemical regulators used in epigenetic studies and propose strategies for developing novel regulators using multidisciplinary approaches.

关键词： chemical genetics epigenetics phytohormone plant growth and development plant growth regulator plasticity

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To curve for survival:Apical hook development

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Journal of Integrative plant Biology 2023年第2期65卷 324-342页

作者： Yichuan Wang Yang Peng Hongwei Guo Department of Biology School of Life SciencesKey Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education InstitutesInstitute of Plant and Food ScienceSouthern University of Science and Technology(SUSTech)Shenzhen 518055China

Apical hook is a simple curved structure formed at the upper part of hypocotyls when dicot seeds germinate in *** hook structure is transient but essential for seedlings’survival during soil emergence due to its efficient protection of the delicate shoot apex from mechanical *** a superb model system for studying plant differential growth,apical hook has fascinated botanists as early as the Darwin age,and significant advances have been achieved at both the morphological and molecular levels to understand how apical hook development is ***,we will mainly summarize the research progress at these two *** will also briefly compare the growth dynamics between apical hook and hypocotyl gravitropic bending at early seed germination phase,with the aim to deduce a certain consensus on their ***,we will outline the remaining questions and future research perspectives for apical hook development.

关键词： apical hook auxin gradient differential growth HOOKLESS1 phytohormones

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The Features and Regulation of Co-transcriptional Splicing in Arabidopsis

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Molecular plant 2020年第2期13卷 278-294页

作者： Danling Zhu Fei Mao Yuanchun Tian Xiaoya Lin Lianfeng Gu Hongya Gu Li-jia Qu Yufeng Wu Zhe Wu sustech-pku institute of plant and food science Department of BiologySouthern University of Science and TechnologyShenzhen 518055China State Key Laboratory for Crop Genetics and Germplasm Enhancement Bioinformatics CenterNanjing Agriculture UniversityNanjingJiangsu 210095China State Key Laboratory of Protein and plant Gene Research Peking-Tsinghua Center for Life SciencesCollege of Life SciencesPeking UniversityBeijing 100871China Basic Forestry and Proteomics Research Center College of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China Present address:School of Life sciences Guangzhou UniversityGuangzhou 510006China

Precursor mRNA(pre-mRNA)splicing is essential for gene expression in most eukaryotic *** studies from mammals,Drosophila,and yeast show that the majority of splicing events occurs *** plants,however,the features of co-transcriptional splicing(CTS)and its regulation still remain largely ***,we used chromatin-bound RNA sequencing to study CTS in Arabidopsis *** found that CTS is widespread in Arabidopsis seedlings,with a large proportion of alternative splicing events determined *** efficiency correlated with gene expression level,the chromatin landscape and,most surprisingly,the number of introns and exons of individual genes,but is independent of gene *** combination with enhanced crosslinking and immunoprecipitation sequencing analysis,we further showed that the hnRNP-like proteins RZ-1B and RZ-1C promote efficient CTS globally through direct binding,frequently to exonic ***,this general effect of RZ-1B/1C on splicing promotion is mainly observed at the chromatin level,not at the mRNA ***-1C promotes CTS of multiple-exon genes in association with its binding to regions both proximal and distal to the regulated *** propose that RZ-1C promotes efficient CTS of genes with multiple exons through cooperative interactions with many exons,introns,and splicing *** work thus reveals important features of CTS in plants and provides methodologies for the investigation of CTS and RNA-binding proteins in plants.

关键词： co-transcriptional splicing CB-RNA-seq eClip-seq RNA binding protein chromatin transcription

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A molecular framework for leaf senescence and plant immunity

A molecular framework for leaf senescence and plant immunity

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第三届全国植物开花·衰老与采后生物学大会

作者： Yi Zhang Hou-Ling Wang Zhonghai Li Hongwei Guo Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry University institute of plant and food science Department of Biology Southern University of Science and Technology（SUSTech）

Senescence is the final stage of leaf development and is triggered by many intrinsic and extrinsic factors. Here, we characterized that ALD1 and FMO1 are two senescence-associated genes（SAGs） and positively regulates leaf senescence in Arabidopsis. Overexpression of ALD1 or FMO1 accelerated leaf senescence in an age-dependent manner, while loss-of-function of ALD1 or FMO1 delayed this process. Application of Pip could accelerate leaf senescence in wild type and ald1 mutant, but could not act in the fmo1 mutant, suggesting that NHP might play an important role during leaf senescence. Strikingly, the expression of ALD1 and FMO1 was remarkedly regulated by ORE1 and ORS1, which are two key transcription factors involved in regulation of age-dependent leaf senescence, implying a possible role of ORE1/ORS1 in plant immunity. Indeed, single mutant nac2-1 and ors1-1 and double mutant nac2-1 ors1-1 showed an enhanced susceptibility Pseudomonas syringae pv. tomato（Pst） DC3000. Moreover, we observed that the Arabidopsis resistance to Pst DC3000 gradually increased during leaf development. Taken together, our work proposes that leaf senescence functions as a positive physiological response for plant immunity.

关键词： Leaf Senescence plant immunity ORE1/ORS1 ALD1/FMO1 Arabidopsis

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Growth asymmetry precedes differential auxin response during apical hook initiation in Arabidopsis

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Journal of Integrative plant Biology 2022年第1期64卷 5-22页

作者： Yang Peng Dan Zhang Yuping Qiu Zhina Xiao Yusi Ji Wenyang Li Yiji Xia Yichuan Wang Hongwei Guo Department of Biology Institute of Plant and Food ScienceSchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Department of Biology Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education InstitutesSchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Department of Biology Faculty of ScienceHong Kong Baptist UniversityKowloon TongHong Kong 999077China Microlens Technologies Beijing 100086China

The development of a hook-like structure at the apical part of the soil-emerging organs has fascinated botanists for centuries,but how it is initiated remains ***,we demonstrate with highthroughput infrared imaging and 2-D clinostat treatment that,when gravity-induced root bending is absent,apical hook formation still takes *** such scenarios,hook formation begins with a de novo growth asymmetry at the apical part of a straightly elongating ***,suchde novo asymmetric growth,but not the following hook enlargement,precedes the establishment of a detectable auxin response asymmetry,and is largely independent of auxin biosynthesis,transport and ***,we found that functional cortical microtubule array is essential for the following enlargement of hook *** microtubule array was disrupted by oryzalin,the polar localization of PIN proteins and the formation of an auxin maximum became impaired at the to-be-hook *** together,we propose a more comprehensive model for apical hook initiation,in which the microtubuledependent polar localization of PINs may mediate the instruction of growth asymmetry that is either stochastically taking place,induced by gravitropic response,or both,to generate a significant auxin gradient that drives the full development of the apical hook.

关键词： apical hook initiation asymmetric auxin response cortical microtubule array de novo growth asymmetry PINs

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High ambient temperature antagonizes ethylene-induced exaggerated apical hook formation in etiolated Arabidopsis seedlings

High ambient temperature antagonizes ethylene-induced exagge...

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第七届长三角植物科学研讨会暨青年学术报告会

作者： Huanhuan Jin Lei Pang Shuang Fang Jinfang Chu Ruixi Li Ziqiang Zhu Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life SciencesNanjing Normal University Department of Biology Institute of Plant and Food ScienceSouthern University of Science and Technology(SUSTech) National Centre for plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Ambient temperature affects plant growth and *** is well established that high ambient temperature induces cell elongation in light-grown Arabidopsis thaliana seedlings by stimulating auxin ***,how etiolated seedlings respond to elevated ambient temperature is still *** apical hook of etiolated seedlings is an essential structure that enables epigeal plants to protrude through the ***,we show that in darkness,high ambient temperature suppresses the ethylene-triggered exaggerated hook formation in wild-type plants and reduces the hook curvatures in constitutively ethylene responsive *** find that high temperature does not affect the activity of the central transcription factor *** hook formation by ethylene is a result of asymmetric auxin distribution,which causes the differential cell *** show that the natural auxin(indole-3-acetic acid,IAA) levels in the hook regions are reduced and their asymmetric distributions are disturbed upon high temperature *** auxin concentrations or blocking auxin transport causes seedlings insensitive to high temperature,supporting that adequate local auxin activity is required for thermo-sensory hook ***,we monitor the transcription of several genes encoding auxin biosynthesis related enzymes and find that most YUCCA genes are transcriptionally down-regulated by elevating ***,we find that the currently reported plant thermo-sensory components in light-grown plants(phytochrome or PIF4) are not sufficient for thermo sensing in etiolated *** speculate that in darkness,plants sense high ambient temperature through a distinct *** together,we demonstrate that high temperature suppresses ethylene-induced exaggerated hook formation via the inhibition of local auxin activities.

关键词： heat YUCCA apical hook hormones ethylene

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Senescence-associated Alternative Splice Variant of NAC Transcription Factor Impacts Leaf Senescence in Populus

Senescence-associated Alternative Splice Variant of NAC Tran...

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第三届全国植物开花·衰老与采后生物学大会

作者： Houling Wang Yi Zhang Ting Wang Qi Yang Yanli Yang Weilun Yin Xinli Xia Hongwei Guo Zhonghai Li Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry University College of Biological sciences and Technology National Engineering Laboratory for Tree Breeding Beijing Forestry University institute of plant and food science Department of Biology Southern University of Science and Technology (SUSTech)

Leaf senescence is the final stage of leaf development involving nutrient recycling from leaves to other organs and is critical for plants’ fitness. Impressive progresses have been achieved in understanding of leaf senescence through genetic studies of senescence mutants and identification of senescence-associated genes using omics-based technologies in annual plant Arabidopsis. However, the underlying molecular regulatory mechanisms of leaf senescence in woody perennial plants remain largely unknown. Here, we perform RNA sequencing analysis to obtain a high-resolution temporal profile of gene expression during autumn leaf senescence in Populus tomentosa. Co-expression network analysis of senescence-associated NAC transcription factors(SenNAC-TF) identifies three hub genes PtRD26, PtNAC055 and PtNAC076, which promote leaf senescence when overexpression. Interestingly, a splicing variant of PtRD26, PtRD26, occurs in an age-dependent manner and functions as a dominant negative regulator of leaf senescence via physically interacting with PtRD26 and repressing its functions by disrupting the binding to downstream target genes. Our work reveals that intron-retained splicing variant provides an additional level of regulation on leaf senescence.

关键词： Leaf Senescence SAGs Alternative Splicing PtRD26 Populus

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Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a live biotherapeutic for phenylketonuria

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science China(Life sciences) 2023年第1期66卷 127-136页

作者： Yu Jiang Bingbing Sun Fenghui Qian Feng Dong Chongmao Xu Wuling Zhong Rui Huang Qiwei Zhai Yu Jiang Sheng Yang Shanghai Taoyusheng Biotechology Co. Ltd.Shanghai 201201China CAS Key Laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Shanghai Research and Development Center of Industrial Biotechnology Shanghai 201201China CAS Key Laboratory of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China

Phenylketonuria(pku),a disease resulting in the disability to degrade phenylalanine(Phe)is an inborn error with a 1 in 10,000 morbidity rate on average around the world which leads to *** an potential alternative to a protein-restricted diet,oral intake of engineered probiotics degrading Phe inside the body is a promising treatment,currently at clinical stage II(Isabella,et al.,2018).However,limited transmembrane transport of Phe is a bottleneck to further improvement of the probiotic’s ***,we achieved simultaneous degradation of Phe both intracellularly and extracellularly by expressing genes encoding the Phe-metabolizing enzyme phenylalanine ammonia lyase(PAL)as an intracellularly free and a cell surface-immobilized enzyme in Escherichia coli Nissle 1917(EcN)which overcomes the transportation *** metabolic engineering strategy was also combined with strengthening of Phe transportation,transportation of PAL-catalyzed trans-cinnamic acid and fixation of released *** of our final synthetic strain TYS8500 with PAL both displayed on the cell surface and expressed inside the cell to the Pah^(F263S)pku mouse model reduced blood Phe concentration by 44.4%compared to the control Ec N,independent of dietary protein ***8500 shows great potential in future applications for pku therapy.

关键词： phenylalanine ammonia lyase cell surface display phenylketonuria TYS8500 oral administration

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