The Features and Regulation of Co-transcriptional Splicing in Arabidopsis
作者机构：SUSTech-PKU Institute of Plant and Food ScienceDepartment of BiologySouthern University of Science and TechnologyShenzhen 518055China State Key Laboratory for Crop Genetics and Germplasm EnhancementBioinformatics CenterNanjing Agriculture UniversityNanjingJiangsu 210095China State Key Laboratory of Protein and Plant Gene ResearchPeking-Tsinghua Center for Life SciencesCollege of Life SciencesPeking UniversityBeijing 100871China Basic Forestry and Proteomics Research CenterCollege of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China Present address:School of Life SciencesGuangzhou UniversityGuangzhou 510006China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2020年第13卷第2期
页 面：278-294页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：supported by Guangdong Innovative and Entrepreneurial Research Team Program(2016ZT06S172) the Shenzhen Sci-Tech Fund No.KYTDPT20181011104005 the National Natural Science Foundation of China(31771365 to Z.W.and 31800268 to D.Z.)
主 题：co-transcriptional splicing CB-RNA-seq eClip-seq RNA binding protein chromatin transcription
摘 要：Precursor mRNA(pre-mRNA)splicing is essential for gene expression in most eukaryotic *** studies from mammals,Drosophila,and yeast show that the majority of splicing events occurs *** plants,however,the features of co-transcriptional splicing(CTS)and its regulation still remain largely ***,we used chromatin-bound RNA sequencing to study CTS in Arabidopsis *** found that CTS is widespread in Arabidopsis seedlings,with a large proportion of alternative splicing events determined *** efficiency correlated with gene expression level,the chromatin landscape and,most surprisingly,the number of introns and exons of individual genes,but is independent of gene *** combination with enhanced crosslinking and immunoprecipitation sequencing analysis,we further showed that the hnRNP-like proteins RZ-1B and RZ-1C promote efficient CTS globally through direct binding,frequently to exonic ***,this general effect of RZ-1B/1C on splicing promotion is mainly observed at the chromatin level,not at the mRNA ***-1C promotes CTS of multiple-exon genes in association with its binding to regions both proximal and distal to the regulated *** propose that RZ-1C promotes efficient CTS of genes with multiple exons through cooperative interactions with many exons,introns,and splicing *** work thus reveals important features of CTS in plants and provides methodologies for the investigation of CTS and RNA-binding proteins in plants.
维普期刊数据库