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Phylogenetic insights resolve Dacampiaceae(Pleosporales)as polyphyletic:Didymocyrtis(Pleosporales,Phaeosphaeriaceae)with Phoma-like anamorphs resurrected and segregated from Polycoccum(Trypetheliales,Polycoccaceae ***.)

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Fungal Diversity 2015年第5期74卷 53-89页

作者： Damien Ertz Paul Diederich James D.Lawrey Franz Berger Colin E.Freebury Brian Coppins Alain Gardiennet Josef Hafellner Department Bryophytes-Thallophytes(BT) Botanic Garden MeiseNieuwelaan 38B-1860 MeiseBelgium Musée national d’histoire naturelle 25 rue MunsterL-2160 LuxembourgLuxembourg Department of Biology George Mason University4400 University DriveFairfaxVA 22030USA Raiffeisenweg 130 A-4794 KopfingAustria canadian Museum of Nature PO Box 3443Stn.BDOttawaOntarion K1P 6P4Canada royal Botanic Garden Edinburgh 20A Inverleith RowEdinburgh EH35LRUK 14 rue Roulette F-21260 VéronnesFrance Institute for Plant Sciences NAWI GrazKarl-Franzens-University GrazHolteigasse 6A-8010 GrazAustria

A phylogenetic analysis of nuLSU and ITS sequences representing genera previously included in Dacampiaceae indicates that the family is strongly polyphyletic and that the type species of Dacampia is placed in *** genus Munkovalsaria *** placed in Didymosphaeriaceae(Pleosporales).Polycoccum *** two species of Clypeococcum are shown to form a new lineage sister to the Trypetheliaceae in Trypetheliales and described here as *** members of Polycoccum *** included in the Pleosporales and are closely related to lichenicolous Phoma-like species of the family *** genus Didymocyrtis is resurrected for these species and for lichenicolous species previously assigned to Diederichia,Diederichomyces,Leptosphaeria and *** genera Diederichia and Diederichomyces are synonymized with *** new combinations Didymocyrtis bryonthae,***,***,***,***,***,***,***,*** and *** are made,and the new name *** is introduced for Phoma *** anamorph-teleomorph relationships are resolved,such as Didymocyrtis ramalinae–Phoma ficuzzae and Didymocyrtis consimilis–Phoma caloplacae,the phylogenetic results being supported by single ascospore cultures that lead to the asexual stage producing pycnidia and conidia in *** by host switching is assumed to be important in the genus *** identification key to Didymocyrtis species is provided.

关键词： Dothideomycetes Leptosphaeria Diederichia Diederichomyces Lichenicolous fungi Phylogeny

酪氨酸激酶抑制剂伊马替尼对K562细胞的DNA损伤修复功能的影响

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中华检验医学杂志 2008年第9期31卷 1034-1038页

作者：史梦冯文莉黄世峰曾建明王小中温健萍张文萍陶昆陈新敏曹唯希黄宗千重庆医科大学医学检验系临床血液学教研室临床检验诊断学教育部重点实验室 400016 canadian Blood services 重庆医科大学附属第一医院血液科

目的建立人类慢性粒细胞白血病（CML）细胞系K562细胞在伊马替尼（Imatinib mesylate）处理前、后的DNA损伤模型，探讨伊马替尼对K562细胞的DNA损伤修复功能的影响。方法用噻唑蓝（MTT）法确定伊马替尼预处理K562细胞的浓度；用免疫印... 详细信息

目的建立人类慢性粒细胞白血病（CML）细胞系K562细胞在伊马替尼（Imatinib mesylate）处理前、后的DNA损伤模型，探讨伊马替尼对K562细胞的DNA损伤修复功能的影响。方法用噻唑蓝（MTT）法确定伊马替尼预处理K562细胞的浓度；用免疫印迹法（Western blot）检测伊马替尼处理后K562细胞BCR／ABL的磷酸化状态，以反映BCR-ABL酪氨酸激酶活性受抑制情况；用彗星实验检测不同浓度过氧化氢（H2O2）诱导的K562细胞、伊马替尼预处理K562细胞的DNA损伤模型；用彗星实验对各组细胞在DNA损伤后的修复进行动态观测。结果MTT实验结果显示，伊马替尼预处理K562细胞的最佳终浓度为1μmol／L，作用时间24h；Westernblot实验结果显示，该浓度的伊马替尼可有效抑制BCR／ABL融合蛋白第177位酪氨酸激酶磷酸化，密度比为0．100±0．018，与对照组（0．425±0．039）相比，降低了（77．11±5．59）％，差异有统计学意义（t=4．57，P〈0．05）；彗星实验确定了各组细胞DNA损伤模型的建立条件，采用10μmol／L终浓度的H2O2对K562细胞和伊马替尼预处理后K562细胞进行染毒，H2O2作用温度和时间为4℃、10min。修复结果显示，经伊马替尼预处理的K562细胞修复时间为120min，与未处理组修复时间60min相比，前者DNA损伤修复时间明显延长（F=97．79，P〈0．05）。结论本研究建立了伊马替尼处理前、后的白血病K562细胞的DNA损伤模型，BCR／ABL融合蛋白的酪氨酸激酶抑制剂伊马替尼能减弱K562细胞的DNA损伤后修复能力。

关键词：白血病,髓样,慢性 K562细胞哌嗪类嘧啶类 DNA修复

Compensation for forest ecological services in China

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Forestry Studies in China 2007年第1期9卷 68-79页

作者： Liu Can Wang Sen Zhang Wei Liang Dan China National Forestry Economics Development Research Center Beijing 100714 E R. China Ppacific Forestry Centre Canadian Forest Service Natural Resources Canada 506 West Burnside Road Victoria B C Canada Department of Agricultural Economics Michigan State University MI 48824-1039 USA

The paper discusses methods to compensate for the costs incurred in the supply of forest ecological services （FES）, i.e. government dominated and market-based instruments as well as progress made so far in China. Fa... 详细信息

关键词： forest ecological services (FES) compensation mechanism forest economics policy

1999～2006年急性冠脉综合征患者死亡及心力衰竭发生率降低

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美国医学会杂志（中文版） 2007年第6期26卷 323-330页

作者： Keith A. A. Fox Philippe Gabriel Steg Kim A. Eagle Shaun G. Goodman Frederick A. Anderson Christopher B. Granger Marcus D. Flather Andrzej Budaj Ann Quill Joel M. Gore 康俊萍（译）马长生（校） Cardiovascular Research Division of Medical and Radiological Sciences The University of Edinburgh Edinburgh Scotland Hospital Bichat Paris France University of Michigan Health System Ann Arbor canadian Heart Research Centre and Terrence Donnelly Heart Centre Division of Cardiology St Michael＇s Hospital University of Toronto Toronto Ontario University of Massachusetts medical School Worcester royal Brompton ＆ Harefield NHS Trust London England Postgraduate medical School Grochowski Hospital Warsaw Poland Duke University medical Center Durham NC 不详

背景：有关药物和介入治疗对ST段抬高型和非ST段抬高型急性冠脉综合征（non—ST-segment elevation acute coronary syndrome，NSTE ACS）患者的作用，随机试验已经提供了足够的证据，但是是否已经转化为相应的临床变化还不清楚。目的... 详细信息

背景：有关药物和介入治疗对ST段抬高型和非ST段抬高型急性冠脉综合征（non—ST-segment elevation acute coronary syndrome，NSTE ACS）患者的作用，随机试验已经提供了足够的证据，但是是否已经转化为相应的临床变化还不清楚。目的：确定ST段抬高型心肌梗死（ST-segment elevation myocardial infarction，STEMI）和NSTE ACS患者的院内治疗改变是否伴随临床结果的改善。设计、地点及患者：全球急性冠脉事件注册研究（Global Registry of Acute Coronary Events，GRACE）是一项多国家队列研究。自1999年7月1日至2006年12月31日，该研究共入选并随访了来自14个国家、113家医院的44372例ACS患者。主要观测指标：当前循证药物和介入治疗应用的趋势及患者预后（死亡、充血性心力衰竭、肺水肿、心源性休克、卒中和心肌梗死）。结果：随着研究时间的进展，相关药物的应用增加（β-受体阻滞剂、他汀类药物、血管紧张素转换酶抑制剂、行或未行经皮冠状动脉介入治疗[percutaneous coronary intervention，PCI]患者应用噻氯毗啶、糖蛋白Ⅱb／Ⅲa抑制剂和低分子肝素；P〈0．001）。STEMI患者药物再灌注治疗下降了22个百分点（95％可信区间[confidence interval，CI]，-27～-17），而直接PCI增加了37个百分点（95％CI，33—41）。在NSTEMI患者中，PCI的比例显著升高了18个百分点（95％CI，15～20）。充血性心力衰竭和肺水肿在STEMI患者中降低了9个百分点（95％CI，-12～-6），在NSTEACS患者中降低了6．9个百分点（95％CI，-8．4～-4．7）。STEMI患者的院内死亡率降低了18个百分点（95％CI，-5．3～-1．9），心源性休克降低了24个百分点（95％CI，-4．3～-0．5）。校正了危险评分后，NSTEACS患者的院内死亡率降低了0．7个百分点（95％CI，-1．7～0．3）；STEMI患者6个月随访时卒中发生率降低了0．8个百分点（95％CI，-1．7～0．1），心肌梗死发生率降低了2．8个百分点（95％CI，-6．4～0．9）。NSTEACS患者6个月死亡率降低了1．6个百分点（95％CI，-3．0～-0．1），卒中发生率降低了0．7个百分点（95％CI，-1．4～0．1）。结论：在本项多国家观察性研究中，ACS患者治疗的改善与6个月新发心力衰竭、死亡率、卒中和心肌梗死发生率显著降低相关。

关键词：充血性心力衰竭急性冠脉综合征院内死亡率发生率经皮冠状动脉介入治疗血管紧张素转换酶抑制剂糖蛋白Ⅱb/Ⅲa抑制剂非ST段抬高型

Particulate matters collected from ceramic factories in Lampang Province affecting rat lungs

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第1期15卷 75-83页

作者： Duriya FONGMOON Surathat PONGNIKORN Aphiruk CHAISENA Sitthichai IAMSAARD Department of medical services Lampang Cancer Hospital Ministry of Public Health Lampang 52000 Thailand Department of Applied Chemistry and Centre for Innovation in Chemistry Faculty of Science Lampang Rajabhat University Lampang 52100 Thailand Department of Anatomy and Integrative Complementary Alternative Medicine (ICAM) Research and Development Group Faculty of Medicine Khon Kaen University Khon Kaen 40002 Thailand

Background： Lung cancer ranks as the fifth largest of all cancer cases in Thailand. However, it is the first leading cancer in the northern part of Thailand （data from 2003-2007）. There are several predisposing causes that lead to lung cancer and one important inducement is particulate matters （PMs）. Lampang Province in Thailand is famous for the ceramic industry, where there are over 200 ceramic industrial factories. PMs are produced during the ceramic manufacturing process and spread throughout all of the working areas. It is very possible that workers could directly inhale PM-contaminated air during working hours. Objective： This study focuses on the toxic effects of PMs collected from ceramic factories on genes and lungs of rats. Methods： PMs collected from six ceramic factories in Lampang Province were extracted using dimethyl sulfoxide （DMSO）. The inductively coupled plasma mass spec- trometry （ICP-MS） and inductively coupled plasma optical emission spectrometry （ICP-OES） were used to analyze the chemical elements at lower and higher concentrations, respectively. Then, the toxicity of PMs on the genes was ex- amined by the Ames test, and subsequently, the effect of PMs on DNA was examined by quantifying the amount of 8-hydroxy-2＇-deoxyguanosine （8-OHdG）. Finally, the toxicity of the PMs on rat＇s lungs was examined by histology. Results： As chemical elements of lower concentrations, cadmium, chromium, nickel, copper, and lead were detected by ICP-MS. As chemical elements of higher concentrations, manganese, magnesium, zinc, iron, potassium, calcium, and sodium were detected by ICP-OES. No mutagenicity in Salmone/la typhimurium was found in the PM extracts from all six factories by utilizing the Ames test. In the histological study, the reduction in spaces of alveolar ducts and sacs, and terminal bronchioles, the thickening of interstitial connective tissues were noted by PM extracts in high amounts （100 and 350 pg）. Female rats were more sensitive to PM extract

关键词： Particulate matter (PM) Ceramic factory Lampang Province Rat lung

bcr-abl反转录病毒介导的慢性粒细胞白血病样小鼠二次移植模型的建立

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肿瘤 2009年第6期29卷 508-513页

作者：张文萍黄世峰袁颖王雅珍温健萍曹唯希黄宗干冯文莉重庆医科大学临床血液学教研室临床检验诊断学教育部重点实验室重庆400016 重庆医科大学临床医学系重庆400016 canadian Blood services Hamilton 22373 重庆医科大学附属第一医院血液科重庆400016

目的:构建bcr-abl反转录病毒介导的小鼠慢性粒细胞白血病(chronic myeloid leukemia,CML)样二次移植模型,为深入研究CML的发病和治疗机制奠定可靠的基础。方法:收集已成功建模的由bcr-abl反转录病毒介导的CML样模型小鼠的骨髓细胞或脾细... 详细信息

目的:构建bcr-abl反转录病毒介导的小鼠慢性粒细胞白血病(chronic myeloid leukemia,CML)样二次移植模型,为深入研究CML的发病和治疗机制奠定可靠的基础。方法:收集已成功建模的由bcr-abl反转录病毒介导的CML样模型小鼠的骨髓细胞或脾细胞,经尾静脉输注入经亚致死剂量γ射线(450 cGy)照射的同种雌性受体鼠,进行造血组织的形态学观察、Y染色体及其特异性性别决定基因Sry的检测以及bcr-abl mRNA和蛋白水平表达的检测,分析鉴定CML样小鼠二次移植模型情况。结果:移植后12 d,肉眼可见明显的脾结节形成,连续切片可见成堆脾集落。移植4~5周后,在雌性受体小鼠骨髓中检出相似Y染色体及Y染色体特异性Sry基因,骨髓和脾脏中检出bcr-abl融合基因。移植10~12周后外周血白细胞水平升高至正常未处理小鼠的4~8倍,粒系细胞水平明显升高至(20~32)×109个/L,外周血涂片幼稚细胞占10%~20%;骨髓涂片结果显示粒系细胞显著增多,易见幼稚细胞,肝脾也可见白血病细胞浸润;同时,在肝脏中检测出bcr-abl融合基因,骨髓中检测出bcr-abl融合蛋白的表达。移植18周后,外周血涂片幼稚细胞占30%以上,小鼠发生急性变,最终因肺出血相继死亡。结论:本研究成功建立了bcr-abl反转录病毒介导的小鼠CML样二次移植模型,该模型可用于后续CML信号通路和治疗机制的研究。

关键词：白血病髓样慢性融合蛋白质类 bcr—abl 异种移植模型抗肿瘤试验小鼠近交BALB/c

Optimising mesh size with escape gaps in a dual-species portunid-trap fishery

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Aquaculture and Fisheries 2020年第6期5卷 308-316页

作者： Matt K.Broadhurst Daniel J.Tolhurst Brian Hughes Vincent Raoult Timothy M.Smith Troy F.Gaston NSW Department of Primary Industries Fisheries Conservation Technology UnitNational Marine Science CentrePO Box 4321Coffs HarbourNSW2450Australia Marine and Estuarine Ecology Unit School of Biological SciencesUniversity of QueenslandBrisbaneQLD4072Australia The Roslin Institute and royal(Dick)School of Veterinary Studies University of EdinburghEaster BushMidlothianEH259RGUnited Kingdom Hunter Local Land services PO Box 440TareeNSW2430Australia School of Environmental and Life Sciences University of NewcastleOurimbahNSW2258Australia

In south-eastern Australia,the same baited,round traps(comprising 50–57-mm mesh netting)are used to target giant mud,Scylla serrata and blue swimmer crabs,Portunus armatus in spatially separated *** fisheries are characterised by the common,problematic discarding of undersized portunids(<85 and 65 mm carapace length;CL for *** and ***)and fish(yellowfin bream,Acanthopagrus australis).This poor selectivity was addressed here in two experiments assessing the utility of(1)traps partially or completely covered in larger mesh(91 mm to match the minimum legal size of the smaller ***),and then(2)any cumulative benefits of fitting species-specific escape *** experiment 1,there were no differences among catches of legal-sized portunids associated with either partial,or complete trap coverage with larger *** of mesh coverage,both designs of 91-mm traps also retained significantly fewer(by up to 42%)undersized *** and *** experiment 2,replicate traps completely covered in 91-mm mesh were tested against conventional traps comprising 56-mm mesh,and traps with the same mesh sizes,but also three escape gaps configured for either ***(46×120 mm)or ***(36×120 mm)(*** treatments in total).All modified traps maintained catches of legal-sized ***,and only the 91-mm traps with escape gaps caught fewer legal-sized *** undersized ***,*** and ***(mean catches reduced by up to 49%)were retained in all larger-meshed than small-meshed traps,and in all of those traps with escape gaps(by up to 95%)than *** there were no significant cumulative benefits of escape gaps in largermeshed traps(measured by a statistical interaction),there was a trend of fewer unwanted catches *** data support configuring portunid traps with mesh sizes matching the morphology of the smallest legal-sized target ***,simply retroactively fitting escape gaps in existing,smaller-m

关键词： Bycatch Escape gap Mesh size Portunid

PTD-OD-HA融合蛋白对BCR-ABL阳性细胞增殖的影响

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解放军医学杂志 2010年第7期35卷 849-852,856页

作者：黄峥兰季茂胜袁颖黄世峰刘钉宾曾建明温健萍冯文莉重庆医科大学临床血液学教研室临床检验诊断学教育部重点实验室重庆400016 重庆医科大学附属第一医院普外科重庆410006 canadian Blood services Hamilton ON LSS 1H8 Canada

目的研究PTD-OD-HA融合蛋白对BCR-ABL阳性细胞株增殖的影响。方法将前期制备的PTD-OD-HA融合蛋白作用于两种BCR-ABL阳性细胞株BaF3-P210和K562,同时设立PBS和OD-HA融合蛋白对照组,用MTT法检测细胞的生长情况。48h后离心收集细胞,用瑞氏... 详细信息

目的研究PTD-OD-HA融合蛋白对BCR-ABL阳性细胞株增殖的影响。方法将前期制备的PTD-OD-HA融合蛋白作用于两种BCR-ABL阳性细胞株BaF3-P210和K562,同时设立PBS和OD-HA融合蛋白对照组,用MTT法检测细胞的生长情况。48h后离心收集细胞,用瑞氏染色及透射电镜观察细胞形态改变,流式细胞仪(FCM)检测细胞周期变化,RT-PCR、Westernblot-ting检测c-myc、cyclinD1mRNA及蛋白表达的变化。结果 MTT检测发现,与PBS和OD-HA对照组比较,PTD-OD-HA能抑制BCR-ABL阳性细胞株的生长。瑞氏染色及透射电镜观察见PTD-OD-HA处理的细胞胞质内有大量空泡样变,出现大量的溶酶体,线粒体广泛扩张;FCM检测发现G1期细胞比例增加,S期和G2期细胞比例减少;RT-PCR及Westernblotting检测发现c-myc、cyclinD1的mRNA及蛋白水平均降低。结论 PTD-OD-HA融合蛋白可抑制BCR-ABL阳性细胞生长,使细胞形态发生改变,并使细胞阻滞在G1期,这些改变可能是通过下调c-myc、cyclinD1的表达实现的。

关键词：白血病,髓样,慢性融合蛋白质类,bcr-abl 细胞增殖

Interpretation, Stratification and Evidence for Sequence Variants Affecting mRNA Splicing in Complete Human Genome Sequences

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Genomics, Proteomics & Bioinformatics 2013年第2期11卷 77-85页

作者： Ben C.Shirley Eliseos J.Mucaki Tyson Whitehead Paul I.Costea Pelin Akan Peter K.Rogan Department of Computer Science Middlesex CollegeThe University of Western Ontario Department of Biochemistry Schulich School of Medicine and DentistryThe University of Western Ontario SHARCNET royal Institute of Technology Science for Life Laboratory Cytognomix Inc.

Information theory-based methods have been shown to be sensitive and specific for pre- dicting and quantifying the effects of non-coding mutations in Mendelian diseases. We present the Shannon pipeline software for genome-scale mutation analysis and provide evidence that the soft- ware predicts variants affecting mRNA splicing. Individual information contents （in bits） of refer- ence and variant splice sites are compared and significant differences are annotated and prioritized. The software has been implemented for CLC-Bio Genomics platform. Annotation indicates the context of novel mutations as well as common and rare SNPs with splicing effects. Potential natural and cryptic mRNA splicing variants are identified, and null mutations are distinguished from leaky mutations. Mutations and rare SNPs were predicted in genomes of three cancer cell lines （U2OS, U251 and A431）, which were supported by expression analyses. After filtering, tractable numbers of potentially deleterious variants are predicted by the software, suitable for further laboratory investigation. In these cell lines, novel functional variants comprised 6-17 inactivating mutations, 1 5 leaky mutations and 6-13 cryptic splicing mutations. Predicted effects were validated by RNA-seq analysis of the three aforementioned cancer cell lines, and expression microarray analysis of SNPs in HapMap cell lines.

关键词： Mutation mRNA splicing Information theory Next-generation sequencing Genome interpretation