检索结果-南通市图书馆

利用微流控技术应对COVID-19和其他肺部疾病的全球挑战

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

engineering 2023年第5期24卷 17-20,I0001页

作者： Yuliang Xie Ryan Becker Michael Scott Kayla Bean Tony jun Huang roy j.carver department of biomedical engineering College of EngineeringUniversity of IowaIowa CityIA 52242USA department of biomedical engineering Pratt School of EngineeringDuke UniversityDurhamNC 27710USA department of Mechanical engineering and Materials Science Pratt School of EngineeringDuke UniversityDurhamNC 27710USA

1. Introduction Pulmonary diseases present one of the most severe threats to human society. Since late 2019, the coronavirus disease 2019(COVID-19) pandemic has significantly impacted the lifestyle, culture, and politics of almost everyone in the world. COVID-19 causes severe pulmonary dysfunction, which is a major cause of mortality for those affected [1].

关键词：微流控技术肺部疾病全球挑战

Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2021年第6期7卷 71-85页

作者： Cong Wu Chriss S.M.Chin Qingyun Huang Ho-Yin Chan Xinge Yu Vellaisamy A.L.roy Wen j.Li department of Mechanical engineering City University of Hong KongHong KongChina department of biomedical engineering City University of Hong KongHong KongChina james Watt School of engineering University of GlasgowGlasgow G128QQUK

In vivo,multiple biophysical cues provided by highly ordered connective tissues of the extracellular matrix regulate skeletal muscle cells to align in parallel with one ***,in routine in vitro cell culture environments,these key factors are often missing,which leads to changes in cell ***,we present a simple strategy for using optical media discs with nanogrooves and other polymer-based substrates nanomolded from the discs to directly culture muscle cells to study their response to the effect of biophysical cues such as nanotopography and substrate *** extend the range of study of biophysical cues for myoblasts by showing that they can sense ripple sizes as small as a 100 nm width and a 20 nm depth for myotube alignment,which has not been reported *** results revealed that nanotopography and substrate stiffness regulated myoblast proliferation and morphology independently,with nanotopographical cues showing a higher *** biophysical cues also worked synergistically,and their individual effects on cells were additive;i.e.,by comparing cells grown on different polymerbased substrates(with and without nanogrooves),the cell proliferation rate could be reduced by as much as~29%,and the elongation rate could be increased as much as~116%.Moreover,during myogenesis,muscle cells actively responded to nanotopography and consistently showed increases in fusion and maturation indices of~28%and~21%,***,under electrical stimulation,the contraction amplitude of well-aligned myotubes was found to be almost 3 times greater than that for the cells on a smooth surface,regardless of the substrate stiffness.

关键词： routine stimulation topography

Microscale tissue-engineered models:overcoming barriers to adoption for neural regeneration research

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第3期11卷 386-387页

作者： Michael j.Moore department of biomedical engineering Tulane University

The last decade has seen a steady proliferation in the use of tissue-engineered cell culture systems（Deforest and Anseth,2012）,and these have been put to good use for studying neural axon growth and guidance（Li and Hoffman-Kim,2008;roy et al.,2013）.These systems have been designed to more closely mimic the natural microenvironment of the developing or repairing nervous system and to enable spatiotemporal control over certain aspects of the microenvironment.

关键词： engineered regeneration adoption barriers mimic enable guidance projection friendly overcome

Structure of protein O-mannose kinase reveals a unique active site architecture

在线全文

学校读者我要写书评

暂无评论

Structure of protein O-mannose kinase reveals a unique activ...

A Comprehensive View on the Progress of Organoid Research with an Emphasis on its Relevance to Disease Characterization

中国晶体学会第六届学术年会暨会员代表大会（大分子晶体学分会）

作者： Qinyu Zhu David Venzke Ameya S.Walimbe Mary E.Anderson Qiuyu Fu Lisa Kinch Wei Wang Xing Chen Nick Grishin Niu Huang Liping Yu jack E.Dixon Kevin P.Campbell junyu Xiao The State Key Laboratory of Protein and Plant Gene Research School of Life SciencesAcademy for Advanced Interdisciplinary StudiesPeking-Tsinghua Center for Life SciencesPeking University Howard Hughes Medical Institute Department of Molecular Physiology and BiophysicsDepartment of NeurologyDepartment of Internal MedicineUniversity of Iowa Roy J.and Lucille A.Carver College of Medicine National Institute of Biological Sciences Beijing department of Biophysics University of TexasSouthwestern Medical Center Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking-Tsinghua Center for Life SciencesSynthetic and Functional Biomolecules CenterKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationPeking University Medical Nuclear Magnetic Resonance Facility University of Iowa Roy J.and Lucille A.Carver College of Medicine department of Pharmacology Department of Cellular and Molecular MedicineDepartment of Chemistry and BiochemistryUniversity of California

The"pseudokinase"Sg K196 is a protein O-mannose kinase(POMK)that catalyzes an essential phosphorylation step during biosynthesis of the laminin-binding glycan ***,the catalytic mechanism underlying this activity remains *** we present the crystal structure of Danio rerio POMK in complex with Mg ions,ADP,aluminum fluoride,and

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

biomedical and Environmental Sciences 2023年第10期36卷 959-971页

作者： Chandra KISHORE Vaishali ji Saurav Mallik Ayan MUKHERjI Namrata TOMAR Soumen Kumar Pati LI Ai Min Sinthia roy BANERjEE Soumadip GHOSH Raza Ali NAQVI University of California Irvine Medical CenterOrangeCA 92868USA department of Botany J.D.Women’s CollegeBailey RoadPatna-800023India Harvard T.H.Chan School of Public Health BostonMA 02115USA department of Information Technology Haldia Institute of TechnologyHaldiaWest BengalIndia Medical College of Wisconsin MilwaukeeWI 53226USA department of Bioinformatics Maulana Abul Kalam Azad University of TechnologyNadiaWest BengalIndia Center for Precision Health School of Biomedical InformaticsThe University of Texas Health Science Center at HoustonHoustonTX 77030USA School of Computer Science and engineering Xi’an University of TechnologyXi'an 710048ShaanxiChina department of Information Technology Guru Nanak Institute of TechnologyKolkataWest BengalIndia department of Computer Science and engineering Sister Nivedita UniversityKolkataWest BengalIndia Center for Precision Health School of Biomedical InformaticsUniversity of IllinoisChicago60612USA

INTRODUCTION Organoids are primary tissue or stem cells derived cell aggregates that have the capacity for self-organization,self-renewal,and the capacity to mimic cellular and tissue level *** can overcome the shortc... 详细信息

关键词： functions. overcome shortcomings

Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity

在线全文

学校读者我要写书评

暂无评论

journal of Molecular Cell Biology 2015年第2期7卷 168-179页

作者： Wu Wu Mona N.Rahman jun Guo Natalie roy Lihua Xue Catherine M.Cahill Shetuan Zhang Zongchao jia department of biomedical and Molecular Sciences Queen’s UniversityKingstonOntario K7L 3N6Canada department of Anesthesiology&Perioperative Care University of California IrvineIrvineCAUSA

Otoferlin,an integral membrane protein implicated in a late stage of exocytosis,has been reported to play a critical role in hearing although the underlying mechanisms remain ***,its widespread tissue distribution infers a more ubiquitous role in synaptic vesicle ***,an excitatory neurotransmitter,is converted to its inhibitory counterpart,g-aminobutyric acid(GABA),by L-glutamic acid decarboxylase(GAD),which exists in soluble(GAD67)and membrane-bound(GAD65)*** the first time,we have revealed a close association between otoferlin and GAD65 in both HEK293 and neuronal cells,including SHSY5Y neuroblastoma and primary rat hippocampus cells,showing a direct interaction between GAD65 and otoferlin’s C2 *** primary rat hippocampus cells,otoferlin and GAD65 co-localized in a punctate pattern within the cell body,as well as in the axon along the path of vesicular ***,GABA is virtually abolished in otoferlin-knockdown neuronal cells whereas otoferlin overexpression markedly increases endogenous *** attenuation in otoferlin-knockdown primary cells is correlated with diminished L-type calcium *** previously unknown and close correlation demonstrates that otoferlin,through GAD65,modulates GABAergic *** discovery of otoferlin–GAD65 functional coupling provides a newavenue for understanding the molecular mechanism by which otoferlin functions in neurological pathways.

关键词： otoferlin GAD65 GABA neurotransmitter exocytosis

TCR–pMHC bond conformation controls TCR ligand discrimination

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第3期17卷 203-217页

作者： Dibyendu KSasmal Wei Feng Sobhan roy Peter Leung Yanran He Chufan Cai Guoshuai Cao Huada Lian jian Qin Enfu Hui Hans Schreiber Erin jAdams jun Huang The Pritzker School of Molecular engineering The University of ChicagoChicagoILUSA department of Biochemistry and Molecular Biology The University of ChicagoChicagoILUSA department of Pathology The University of ChicagoChicagoILUSA department of Materials Science&engineering Stanford UniversityStanfordCAUSA department of Chemical engineering Stanford UniversityStanfordCAUSA Section of Cell&Developmental Biology Division of Biological SciencesUniversity of CaliforniaSan DiegoCAUSA

A major unanswered question is how a TCR discriminates between foreign and self-peptides presented on the APC ***,we used in situ fluorescence resonance energy transfer(FRET)to measure the distances of single TCR–pMHC bonds and the conformations of individual TCR–CD3ζreceptors at the membranes of live primary T *** found that a TCR discriminates between closely related peptides by forming single TCR–pMHC bonds with different conformations,and the most potent pMHC forms the shortest *** bond conformation is an intrinsic property that is independent of the binding affinity and kinetics,TCR microcluster formation,and CD4 *** bond conformation dictates the degree of CD3ζdissociation from the inner leaflet of the plasma membrane via a positive calcium signaling feedback loop to precisely control the accessibility of CD3ζITAMs for *** data revealed the mechanism by which a TCR deciphers the structural differences among peptides via the TCR–pMHC bond conformation.

关键词： Bond conformation T cell receptor Single molecule FRET ligand discrimination

Building biological foundries for next-generation synthetic biology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2015年第10期58卷 658-665页

作者： CHAO Ran YUAN YongBo ZHAO HuiMin department of Chemical and Biomolecular engineering University of Illinois at Urbana-Champaign Institute for Genomic Biology University of Illinois at Urbana-Champaign department of Bioengineering Department of Chemistry Center for Biophysics and Computational Biology University of Illinois at Urbana-Champaign

Synthetic biology is an interdisciplinary field that takes top-down approaches to understand and engineer biological systems through design-build-test cycles. A number of advances in this relatively young field have greatly accelerated such engineering cycles. Specifically, various innovative tools were developed for in silico biosystems design, DNA de novo synthesis and assembly, construct verification, as well as metabolite analysis, which have laid a solid foundation for building biological foundries for rapid prototyping of improved or novel biosystems. This review summarizes the state-of-the-art technologies for synthetic biology and discusses the challenges to establish such biological foundries.

关键词： biofabrication automation metabolic engineering biosystems design DNA assembly DNA sequencing metaboliteanalysis

同方期刊数据库

A brief overview of synthetic biology research programs and roadmap studies in the United States

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2016年第4期1卷 258-264页

作者： Tong Si Huimin Zhao Carl R.Woese Institute for Genomic Biology University of Illinois at Urbana-ChampaignUrbanaIL 61801USA Departement of Chemical and Biomolecular engineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA department of Chemistry University of Illinois at Urbana-ChampaignUrbanaIL 61801USA department of Biochemistry University of Illinois at Urbana-ChampaignUrbanaIL 61801USA department of Bioengineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

The United States is a leading nation in the development of synthetic biology,an emerging engineering discipline to create,control and reprogram biological *** strategic investment from its government agencies,the *** established numerous research centers and programs in synthetic biology,enabling significant advances in foundational tool development and practical applications ranging from bioenergy,biomanufacturing,to *** maintain its leadership in synthetic biology,U.S,has conducted several roadmap studies to provide strategic visions and action *** we will provide a brief overview of the major research programs and roadmap studies of synthetic biology in the U.S.

关键词： synthetic maintain brief