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Chinese Journal of Chemical Engineering 2016年第9期24卷 1263-1269页

作者： Zhuhua Chan Runping Wang Fan Yang Runying Zeng State Key Laboratory Breeding Base of Marine Genetic resource Third Institute of OceanographySOAXiamen 361005China South China Sea bio-resource Exploitation and Utilization Collaborative Innovation center Guangzhou 510000China

A metagenomic library recombinant clone CAPL3, an Escherichia coli strain generated by transformed with metagenomic library from deep-sea sediments, can efficiently produce cold active lipase. The effects of both temperature and dissolved oxygen（DO） on cold active lipase production by batch culture of metagenomic library recombinant clone（CAPL3） from deep-sea sediment were investigated. First, a two-stage temperature control strategy was developed, in which the temperature was kept at 34 ℃ for the first 15 h, and then switched to30 ℃. The cold active lipase activity and productivity reached 315.2 U·ml^-1and 8.08 U·ml^-1·h^-1, respectively,increased by both 14.5% compared to the results obtained with temperature controlled at 30℃. In addition, different DO control modes were conducted, based on the data obtained from the different DO control strategies and analysis of kinetics parameters at different DO levels. A step-wise temperature and DO control strategy were developed to improve lipase production, i.e., temperature and DO level were controlled at 34℃, 30% during 0–15 h;30 ℃, 30% during 15–18 h, and 30 ℃, 20% during 18–39 h. With this strategy, the maximum lipase activity reached 354.6 U·ml^-1at 39 h, which was 28.8% higher than that achieved without temperature and DO control（275.3 U·ml^-1）.

关键词： Deep-sea sediments Metagenomic library Cold active lipase Step-wise temperature and DO control strategy

Characterization of an extreme alkaline-stable keratinase from the draft genome of feather-degrading Bacillus ***7 from deep-sea

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Acta Oceanologica Sinica 2019年第2期38卷 87-95页

作者： Min Jin Chen Chen Xiongfei He Runying Zeng State Key Laboratory Breeding Base of Marine Genetic resource Third Institute of Oceanography Ministry of Natural Resources South China Sea bio-resource Exploitation and Utilization Collaborative Innovation center Fujian Collaborative Innovation center for Exploitation and Utilization of Marine biological resources

Bacillus sp. JM7, a strain isolated from the deep-sea of the South China Sea, was found to efficiently degrade 79.4% native chicken feather within 30 h. Scanning electron microscopy analysis showed that JM7 strain could gradually degrade feather by modifying the microstructure of feather keratin. A total of 25 protease genes were predicted from the draft genome of JM7 strain, among which a predicted subtilisin-like serine protease(designated as Ker02562) was further characterized for its keratinolytic activity. The recombinant Ker02562 functioned at a wide range of temperatures from 30℃ to 60℃, with an optimum at 40–50℃. Ker02562 was highly active at various pHs ranging from 5.0 to 13.0, with a maximum activity observed at pH 7.0–9.0. Remarkably, recombinant Ker02562 was stable in extreme alkaline environments(pH 10–13), which was much better than most other reported keratinases. Collectively, these favorable properties could make Bacillus sp. JM7 and Ker02562 attractive to be applied in the detergent formulation and feather bioconversion.

关键词： Bacillus deep-sea feather-degradation keratinases feather bioconversion alkaline-stable

Lignin-derived dual-function red light carbon dots for hypochlorite detection and anti-counterfeiting

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Frontiers of Chemical Science and Engineering 2023年第7期17卷 966-975页

作者： Yixuan Chang Fanwei Kong Zihao Zhu Ziai Wang Chunxia Chen Xiaobai Li Hongwei Ma Key Laboratory of Forest Plant Ecology Ministry of EducationEngineering Research Center of Forest Bio-PreparationCollege of ChemistryChemical Engineering and Resource UtilizationNortheast Forestry UniversityHarbin 150040China State Engineering Laboratory of bio-resource Eco-Utilization Northeast Forestry UniversityHarbin 150040China Collaborative Innovation center for Development and Utilization of Forest resources Harbin 150040China

The efficient utilization of natural lignin,which is the main by-product of the cellulose industry,is crucial for enhancing its economic value,alleviating the environmental burden,and improving ecological *** taking advantage of the large sp2 hybrid domain of lignin and introducing amino functional groups,new lignin-derived carbon dots(SPN-CDs)with red fluorescence were successfully *** with green and blue fluorescent materials,red SPN-CDs have desirable anti-interference properties of short-wave background and exhibit superior luminescence *** SPN-CDs obtained exhibited sensitive and distinctive visible color with fluorescence-dual responses toward *** this feature,a portable,low-cost,and sensitive fluorescence sensing paper with a low limit of detection of 0.249μmol∙L^(–1) was fabricated using the SPN-CDs for hypochlorite ***,a new type of visible-light and fluorescence dual-channel information encryption platform was ***-concentration hypochlorite can be employed as an accessible and efficient information encryption/decryption stimulus,as well as an information“eraser”,facilitating a safe and diversified transmission and convenient decryption of *** work opens new avenues for high-value-added applications of lignin-based fluorescent materials.

关键词： alkali lignin red light carbon dots hypochlorite encryption and anti-counterfeiting

Now and future of mouse mutagenesis for human disease models

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Journal of Genetics and Genomics 2010年第9期37卷 559-572页

作者： Yoichi Gondo Mutagenesis and Genomics Team RIKEN Bio Resource Center

One of the major objectives of the Human Genome Project is to understand the biological function of the gene and genome as well as to develop clinical applications for human diseases. For this purpose, the experimental validations and preclinical trails by using animal models are indispensable. The mouse （Mus musculus） is one of the best animal models because genetics is well established in the mouse and embryonic manipulation technologies are also well developed. Large-scale mouse mutagenesis projects have been conducted to develop various mouse models since 1997. Originally, the phenotype-driven mutagenesis with N-ethyl-N-nitrosourea （ENU） has been the major efforts internationally then knockout/conditional mouse projects and gene-driven mutagenesis have been following. At the beginning, simple monogenic traits in the experimental condition have been elucidated. Then, more complex traits with variety of environmental interactions and gene-to-gene interactions （epistasis） have been challenged with mutant mice. In addition, chromosomal substitution strains and collaborative cross strains are also available to elucidate the complex traits in the mouse. Altogether, mouse models with mutagenesis and various laboratory strains will accelerate the studies of functional genomics in the mouse as well as in human.

关键词： mutagenesis functional genomics reverse genetics model animal quantitative trait epistasis

Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

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Journal of Integrative Plant biology 2011年第1期53卷 63-73页

作者： Kyaw Thu Moe Jong-Wook Chung Young-Il Cho Jung-Kyung Moon Ja-Hwan Ku Jin-Kyo Jung Jungran Lee Yong-Jin Park Department of Plant resources College of Industrial SciencesKongju National University The Rural Development Administration(RDA) Legume bio-resource center of Green Manure(LBRCGM) Kongju National University

Mungbean （Vigna radiata （L.） Wilczek） is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs （〉_500 bp） with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat （SSR） motifs was identified in Jangan （1 630） compared with that of Sunhwa （1 334）. A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms （SNPs） and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was -860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.

关键词： Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean SNPs

Applications of Raman spectroscopy in two-dimensional materials

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Journal of Innovative Optical Health Sciences 2020年第5期3卷 42-62页

作者： Pengkun Yin Qingyu Lin Yixiang Duan College of Life Sciences Research center of Analytical Instrumentation Key Laboratory of bio-resource and Eco-environment Ministry of Education Sichuan University 610064 ChengduP.R.China

At present,two-dimensional(2D)materials have shown great application potential in numerous fields based on their physical chemical and electronic *** spectroscopy and de-rivative techniques are effective tools for characterizing 2D *** spectroscopy conveys lots of knowledge on 2D materials,including layer number,doping type,strain and interlayer *** review summarized advanced applications of Raman spectroscopy in 2D *** challenges and possible applied directions of Raman spectroscopy to 2D materials are discussed in detail.

关键词： Raman spectroscopy and derivative techniques monolayer materials nondestructive characterization

Three new isocoumarin derivatives from the mangrove endophytic fungus Penicillium sp. YYSJ-3

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Chinese Journal of Natural Medicines 2020年第4期18卷 256-260页

作者： QIU Pei CAI Run-Lin LI Lin SHE Zhi-Gang School of Chemistry Sun Yat-Sen UniversityGuangzhou 510275China South China Sea bio-resource Exploitation and Utilization Collaborative Innovation center Guangzhou 510006China

Three new isocoumarin derivatives,(S)-6,8-dihydroxy-5-(methoxymethyl)-3,7-dimethylisochroman-1-one(1),(S)-6,8-dihydroxy-3,5,7-trimethyl-isochroman-1-one(2) and(R)-2-chloro-3-(8-hydroxy-6-methoxy-1-oxo-1 H-isochromen-3-yl) propyl acetate(3), along with four known compounds(4–7) were isolated from a mangrove endophytic fungus Penicillium sp. YYSJ-3. Their structures were established on the basis of the extensive spectroscopic data and HR-ESI-MS analysis. The absolute configurations of1–3 were further determined by X-ray diffraction analysis and optical rotations. Compounds 3, 6 and 7 showed promising inhibitory activity against α-glucosidase, which were stronger than that of the positive control 1-deoxynojirimycin(IC50 141.2 μmol·L-1).

关键词： Isocoumarin derivatives Penicillium sp. Structure elucidation α-Glucosidase inhibitory activity

Metabolic engineering for the microbial production of isoprenoids:Carotenoids and isoprenoid-based biofuels

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Synthetic and Systems biotechnology 2017年第3期2卷 167-175页

作者： Fu-Xing Niu Qian Lu Yi-Fan Bu Jian-Zhong Liu biotechnology Research center and biomedical center School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China South China Sea bio-resource Exploitation and Utilization Collaborative Innovation center School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Isoprenoids are the most abundant and highly diverse group of natural *** isoprenoids have been used for pharmaceuticals,nutraceuticals,flavors,cosmetics,food additives and *** and isoprenoid-based biofuels are two classes of important *** isoprenoids have been produced microbially through metabolic engineering and synthetic biology ***,we briefly review the engineered biosynthetic pathways in well-characterized microbial systems for the production of carotenoids and several isoprenoid-based biofuels.

关键词： Carotenoids Isoprenoid-based biofuel Metabolic engineering Synthetic biology

One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection

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Food Science and Human Wellness 2024年第2期13卷 920-931页

作者： Guobao Ning Quanmei Duan Huan Liang Huifang Liu Min Zhou Chunlan Chen Chong Zhang Hui Zhao Canpeng Li School of Chemical Science and Technology Yunnan UniversityKunming 650091China Laboratory for Conservation and Utilization of bio-resource Yunnan UniversityKunming 650091China Kunming Customs Technology center Kunming 650228China

Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the *** is urgent to identify the pathogenic microorganism of the diarrhea in short *** this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition *** colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/*** the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition *** electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/***'s worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for *** electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.

关键词： Norovirus Specific peptides Electrochemical and colorimetric assay Dual-mode Copper peroxide/covalent organic framework

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M2-like Kupffer cells in fibrotic liver may protect against acute insult

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World Journal of Gastroenterology 2017年第20期23卷 3655-3663页

作者： Qing-Fen Zheng Li Bai Zhong-Ping Duan Yuan-Ping Han Su-Jun Zheng Yu Chen Jian-Sheng Li Department of Gastroenterology First Affiliated Hospital of Zhengzhou University Artificial Liver center Beijing You An HospitalCapital Medical University center for Growth Metabolism and AgingKey Laboratory for Bio-Resource and Eco-EnvironmentCollege of Life SciencesSichuan University National Key Laboratory of biotherapy Sichuan University

AIM To investigate the mechanism of hepatoprotection conferred by liver fibrosis through evaluating the activation phenotype of kupffer *** Control and fibrotic mice were challenged with a lethal dose of D-Gal N/lipopolysaccharide(LPS),and hepatic damage was assessed by histology,serum alanine transferase(ALT)levels,and hepatic expression of HMGB1,a potent pro-inflammatory *** localization of F4/80(a surrogate marker of KCs),HMGB1,and type I collagen(Col-1)was determined by immunofluorescence *** phenotype of KCs was characterized by real-time *** isolated from control or fibrotic mice were challenged with LPS or HMGB1 peptide,and HMGB1 translocation was *** Liver fibrosis protected mice against D-Gal N/LPS challenge,as shown by improved hepatic histology and reduced elevation of ALT compared with the normal mice treated in the same *** hepatoprotection was also accompanied by inhibition of HMGB1 expression in the ***-localization of F4/80,HMGB1,and Col-1 was found in fibrotic livers,indicating the close relationship between KCs,HMGB1 and liver *** isolated from fibrotic mice predominantly exhibited an M2-like *** vitro experiments showed that HMGB1 was localized in the nucleus of the majority of M2-like KCs and that the translocation of HMGB1 was inhibited following stimulation with LPS or HMGB1 peptide,while both LPS and HMGB1 peptide elicited translocation of intranuclear HMGB1 in KCs isolated from the control *** M2-like Kupffer cells in fibrotic liver may exert a protective effect against acute insult by inhibiting the translocation of HMGB1.

关键词： Liver fibrosis Injury resistance Kupffer cell activation High-mobility group box 1 Translocation