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Analysis of the Electrophoretic Profiles of Prion Protein in Carcinous and Pericarcinous Lysates of Six Different Types of Cancers

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Biomedical and Environmental Sciences 2021年第9期34卷 683-692页

作者： WEI Wei WU Yue Zhang XIAO Kang XU Guo Hui SONG Yun Tao SHI Qi DONG Xiao Ping Key Laboratory of Carcinogenesis and Translational research(Ministry of education) Head and Neck Surgery DepartmentPeking University Cancer Hospital&InstituteBeijing 100142China State Key Laboratory for infectious disease prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases(Zhejiang University)National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China center for global Public Health Chinese Center for Disease Control and PreventionBeijing 102206China Wuhan Institute of Virology Chinese Academy of ScienceWuhan 430071Hubei China China Academy of Chinese Medical Sciences Beijing 100700China

Objective To find the different electrophoretic profiles of prion protein in carcinous and individual pericarcinous tissues in lysates of gastric,colon,liver,lung,thyroid,and laryngeal *** Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blot were used to test the amounts and electrophoretic patterns of total Pr P and the tolerance of PK(protease K)digestion among six various cancer tissue *** A mass of Pr P signals with a large molecular weight were identified in the homogenates of peripheral *** amounts and electrophoretic patterns of total Pr P did not differ significantly between carcinous and pericarcinous *** Ps in all types of the tested cancer samples were PK sensitive but showed diversity in the tolerance of PK digestion among various tissue *** The study revealed that the included electrophoretic patterns of carcinous and pericarcinous tissues were almost *** Pr P-specific immunohistochemical assay,evaluation of Pr P electrophoretic patterns in the peripheral organs and tissues by Western blot does not reflect tumor malignancy.

关键词： Prion protein Cancer Electrophoretic profiles

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Epidemiological Characteristics of Human Anthrax-China,2018-2021

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China CDC weekly 2022年第35期4卷 783-787页

作者： Huijuan Zhang Enmin Zhang Mu Guo Jinrong He Wei Li Jianchun Wei National Institute for Communicable disease Control and prevention Chinese Center for Disease Control and PreventionBeijingChina State Key Laboratory of infectious disease prevention and Control BeijingChina Yunnan Institute for Endemic disease Control and prevention Dali CityYunnan ProvinceChina

Introduction:The epidemic of human anthrax is at a low level in China in recent years,but the reported incidence increased in *** order to understand the current landscape of research and knowledge about anthrax in China,the epidemiological characteristics of anthrax in humans from 2018 to 2021 were analyzed and the prevention and control suggestions were ***:Surveillance data of anthrax in humans and livestock,together with human outbreaks data during 2018-2021,were collected and analyzed by descriptive statistics *** number and proportion of outbreaks,cases and deaths by provincial-level administrative divisions(PLADs),clinical types,and contributing factors were ***:A total of 1,244 cases of human anthrax and 53 outbreaks were reported from 2018 to 2021 in *** the incidence of anthrax declined from 2018 to 2020,it increased in *** regions of anthrax were mainly located in the west and the northeast PLADs of China,though cases were reported in some central and eastern PLADs in *** and middle-aged men involved in animal husbandry were found to be at a higher risk of *** the reported outbreaks were associated with the exposure of infected livestock.A total of 296 livestock anthrax cases were ***:The increased incidence and wider geographical distribution of human anthrax in 2021 were found to be the result of inadequate supervision of diseased animals as well as updated diagnostic *** such,the monitoring of risk factors and emergency preparation procedures should be strengthened at the national *** addition,it is also critical to strengthen health education for high-risk occupational groups and strengthen professional training for local ***,more measures should be carried out to strengthen anthrax surveillance in livestock husbandry.

关键词： prevention husbandry strengthened

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The First Detection of Echinococcus Granulosus DNA in Residents’Hands,Dogs’Hair,and Soil in Highly Endemic Region of Echinococcosis-Naqu City,Xizang Autonomous Region,China,2020

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China CDC weekly 2022年第44期4卷 982-985页

作者： Baixue Liu Chuizhao Xue Xu Wang Wenting Wu Shuai Han Weiping Wu National Institute of Parasitic diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesShanghaiChina Institute for infectious disease and Endemic disease Control Beijing Center for Disease Prevention and ControlBeijingChina

Summary What is already known about this topic?Echinococcosis is a serious zoonotic parasitic disease that predominantly infects humans;domestic animals like cattle or sheep;as well as wild animals such as small *** date,there is a lack of comprehensive information about all potential environmental transmission routes for *** is added by this report?This study assesses the importance of under-researched environmental factors for the transmission of *** concludes that hand hygiene is an important factor in the robust environmental transmission of Echinococcus *** are the implications for public health practice?This study suggests that residents are at risk of catching echinococcosis through hand-oral transmission *** education given to local residents is thus a key intervention,as well as avoiding close contact with dogs and ensuring proper hand-washing practices are followed.

关键词： residents Highly education

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Challenges and needs for China to eliminate rabies

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infectious diseases of Poverty 2013年第1期2卷 178-187页

作者： Wenwu Yin Jie Dong Changchun Tu John Edwards Fusheng Guo Hang Zhou Hongjie Yu Sirenda Vong Division of infectious diseases Chinese Center for Disease Control and PreventionBeijingChina Key Laboratory of Surveillance and Early-warning on infectious disease Chinese Center for Disease Control and PreventionBeijingChina World Health Organization Country OfficeBeijingChina OIE Reference Laboratory for Rabies Institute of Military VeterinaryAcademy of Military Medical SciencesChangchunChina Food and Agriculture Organization BeijingChina National Institute for viral disease control and prevention Chinese Center for Disease Control and PreventionBeijingChina

In China,rabies is a significant public health concern where dogs remain the main reservoir of disease transmission to humans;rabies-related mortality ranks second in the *** compiled all published articles and official documents on rabies in China's Mainland to examine challenges and needs to eliminate rabies in the *** Chinese authorities have identified rabies as a priority,recognized rabies control in dogs as key to control rabies in humans and required intersectoral *** have been made to respond effectively to the latest re-emergence of rabies,which peaked in 2007 with>3,300 *** these outcomes and the increasing volume of publications and regulations in the recent years,our review points to some major information gaps to improve rabies control activities and envisage elimination *** emphasis on laboratory or pathogen-associated and basic epidemiology research in the literature has contrasted with the absence of information to monitor various systems in humans and animals(*** of surveillance,response and post-exposure prophylaxis).Information is also lacking to appropriately inform policymakers(*** disease burden,impact of policies)and assist program managers(*** and strategic guidance for cost-effective prevention and control activities,public education and dog population management).In conclusion,strategic planning is needed to provide a sense of direction,demonstrate feasibility of elimination in China,and develop a research agenda,addressing country’s operational needs and *** planning should be a multisectoral effort.

关键词： Rabies Elimination China Needs Challenges Review

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Epizootiological surveillance of porcine circoviruses in free-ranging wild boars in China

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Virologica Sinica 2023年第5期38卷 663-670页

作者： Wenjie Gong Haiying Du Tong Wang Heting Sun Peng Peng Siyuan Qin Haidong Geng Zheng Zeng Wangwang Liang Hongquan Ling Changchun Tu Zhongzhong Tu State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic infectious diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of Zoonosisand College of Veterinary MedicineJilin UniversityChangchun130062China Changchun Veterinary research Institute Chinese Academy of Agricultural SciencesChangchun130122China Jiangsu Co-Innovation center for the prevention and Control of Important Animal infectious disease and Zoonoses Yangzhou UniversityYangzhou225009China Biological Disaster Control and prevention center National Forestry and Grassland AdministrationShenyang110034China Chongqing Animal disease prevention and Control center Chongqing401120China

Four species of porcine circoviruses(PCV1–4)have been reported to circulate in Chinese domestic pigs,while the epizootiology of these viruses in free-ranging wild boars in China remains *** this study,tissue and serum samples collected from diseased or apparently healthy wild boars between 2018 and 2020 in 19 regions of China were tested for the prevalence of PCV1–4 *** rates of PCV1,PCV2,and PCV3 DNA in the tissue samples of Chinese wild boars were 1.6%(4/247),58.3%(144/247),and 10.9%(27/247)respectively,with none positive for *** analysis of viral genome showed that the four PCV1 strains distributed in Hunan and Inner Mongolia shared 97.5%–99.6%sequence identity with global distributed reference *** of the ORF2 gene sequences showed that 80 PCV2 strains widely distributed in 18 regions shared 79.5%–100%sequence identity with reference strains from domestic pigs and wild boars,and were grouped into PCV2a(7),PCV2b(31)and PCV2d(42).For PCV3,17 sequenced strains shared 97.2%–100%nucleotide identity at the genomic level and could be divided into PCV3a(3),PCV3b(2)and PCV3c(12)based on the phylogeny of ORF2 gene *** data revealed antibody positive rates against PCV1 and PCV2 of 11.4%(19/167)and 53.9%(90/167)*** data obtained in this study improved our understanding about the epidemiological situations of PCVs infection in free-ranging wild boars in China and will be valuable for the prevention and control of diseases caused by PCVs infection.

关键词： Porcine circoviruses(PCVs) Epizootiology Wild boar China

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Identification and semisynthesis of(-)-anisomelic acid as oral agent against SARS-CoV-2 in mice

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National Science Review 2022年第11期9卷 29-36页

作者： Hai-Xin Yu Nan Zheng Chi-Tai Yeh Chien-Ming Lee Qi Zhang Wen-Lv Zheng Qing Chang Yuan-He Li Yu-Jun Li Gui-Zhen Wu Jun-Min Quan Lin-Qi Zhang Yew-Min Tzeng Zhen Yang Laboratory of Chemical Genomics School of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School Department of Medicinal research and education Taipei Medical University-Shuang Ho Hospital Department of Applied Science Taitung University center for global Health and infectious diseases Comprehensive AIDS Research Center and Beijing Advanced Innovation Center for Structural Biology School of Medicine Tsinghua University Lanzhou Institute of Separation Science State Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of education and Beijing National Laboratory for Molecular Science(BNLMS) College of Chemistry and Molecular EngineeringPeking University Shenzhen Bay Laboratory NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Department of Applied Chemistry Chaoyang University of Technology

(-)-Anisomelic acid, isolated from Anisomeles indica(L.) Kuntze(Labiatae) leaves, is a macrocyclic cembranolide with a trans-fused α-methylene-γ-lactone motif. Anisomelic acid effectively inhibits SARS-Co V-2 replication and viral-induced cytopathic effects with an EC50 of 1.1 and 4.3 μM, *** studies of SARS-CoV-2-infected K18-hACE2 mice showed that oral administration of anisomelic acid and subcutaneous dosing of remdesivir can both reduce the viral titers in the lung tissue at the same level. To facilitate drug discovery, we used a semisynthetic approach to shorten the project timelines. The enantioselective semisynthesis of anisomelic acid from the naturally enriched and commercially available starting material(+)-costunolide was achieved in five steps with a 27% overall *** developed chemistry provides opportunities for developing anisomelic-acid-based novel ligands for selectively targeting proteins involved in viral infections.

关键词： identification enantioselective semisynthesis (−)-anisomelic acid oral agent anti-SARS-CoV-2

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Beta-and Novel Delta-Coronaviruses Are Identified from Wild animals in the Qinghai-Tibetan Plateau, China

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Virologica Sinica 2021年第3期36卷 402-411页

作者： Wentao Zhu Jing Yang Shan Lu Ruiting Lan Dong Jin Xue-lian Luo Ji Pu Shusheng Wu Jianguo Xu State Key Laboratory of infectious disease prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Shanghai Public Health Clinical center Fudan UniversityShanghai 201508China research Units of Discovery of Unknown Bacteria and Function Chinese Academy of Medical SciencesBeijing 100730China School of Biotechnology and Biomolecular Sciences University of New South WalesSydneyNSW 2052Australia Yushu Prefecture center for disease Control and prevention Yushu 815000China Department of Laboratorial Science and Technology&Vaccine research center School of Public HealthPeking UniversityBeijing 100191China

Outbreaks of severe virus infections with the potential to cause global pandemics are increasingly concerning. One type of those commonly emerging and re-emerging pathogens are coronaviruses(SARS-Co V, MERS-Co V and SARS-Co V-2).Wild animals are hosts of different coronaviruses with the potential risk of cross-species transmission. However, little is known about the reservoir and host of coronaviruses in wild animals in Qinghai Province, where has the greatest biodiversity among the world's high-altitude regions. Here, from the next-generation sequencing data, we obtained a known beta-coronavirus(beta-Co V) genome and a novel delta-coronavirus(delta-Co V) genome from faecal samples of 29 marmots, 50 rats and 25 birds in Yushu Tibetan Autonomous Prefecture, Qinghai Province, China in July 2019. According to the phylogenetic analysis, the beta-Co V shared high nucleotide identity with Coronavirus HKU24. Although the novel delta-Co V(Mt Co V) was closely related to Sparrow deltacoronavirus ISU42824, the protein spike of the novel delta-Co V showed highest amino acid identity to Sparrow coronavirus HKU17(73.1%). Interestingly, our results identified a novel host(Montifringilla taczanowskii) for the novel delta-Co V and the potential cross-species transmission. The most recent common ancestor(t MRCA) of Mt Co Vs along with other closest members of the species of Coronavirus HKU15 was estimated to be 289 years ago. Thus, this study increases our understanding of the genetic diversity of beta-Co Vs and deltaCo Vs, and also provides a new perspective of the coronavirus hosts.

关键词： Coronavirus Qinghai-Tibetan plateau Rat Montifringilla taczanowskii Marmot

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Identifying the spatiotemporal clusters of plague occurrences in China during the Third P Identifying the spatiotemporal clusters of plague occurrences in China during the Third Pandemic andemic

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Integrative Zoology 2020年第1期15卷 69-78页

作者： Zhe SUN Zhibin ZHANG Qiyong LIU Baolei LYU Xiye FANG Shuchun WANG Jianguo XU Lei XU Bing XU Ministry of education Key Laboratory for Earth System Modeling Department of Earth System ScienceTsinghua UniversityBeijingChina Joint center for global Change Studies BeijingChina State Key Laboratory of Integrated Management on Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina State Key Laboratory of infectious disease prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Huayun Sounding Meteorology Technology Corporation BeijingChina Centre for Ecological and Evolutionary Synthesis(CEES) Department of BiosciencesUniversity of OsloOsloNorway

Plague,a devastating infectious disease caused by Yersinia pestis,has killed millions of people in the past and is still active in the natural foci of the world *** the spatiotemporal patterns of plague outbreaks in history is critically important,as it may help to facilitate prevention and control of potential future *** this study,we explored spatiotemporal clusters of human plague occurrences in China using a machine-learning clustering method and reconstructed the potential transmission pattern during the Third Pandemic(1772-1964).We succeeded in identifying 6 clusters in the space domain(2D)and 13 clusters in the spatiotemporal domain(3D).Our results suggest that there were several temporal outbreaks and transmissions of plague in different spatial *** with the spatiotemporal nearest neighbor approach(ST-NNA),this method could allow us to have a clearer look at the spatiotemporal patterns of plague.

关键词： clustering plague transmission spatiotemporal outbreaks Yersinia pestis

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Analysis of the Practice of Community-Based Self-Sampling and Its Implication on prevention and Control of infectious diseases—Fengtai District,Beijing Municipality,China,2022

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China CDC weekly 2023年第10期5卷 213-217页

作者： Yaping Wang Shugang Li Hao Wu Jue Liu Department of Epidemiology and Biostatistics School of Public HealthPeking UniversityBeijingChina School of Public Health Capital Medical UniversityBeijingChina School of General Practice and Continuing education Capital Medical UniversityBeijingChina Institute for global Health and Development Peking UniversityBeijingChina global center for infectious disease and Policy research and global Health and infectious diseases Group Peking UniversityBeijingChina

Summary What is already known about this topic?So far,no descriptive analysis has been conducted on community residents with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid self-sampling in *** is added by this report?This report found that self-sampling had a wide age and regional distribution,with the time from selfsampling to result-reporting typically taking less than one ***,self-sampling was found to save a considerable amount of manpower and medical resources compared to regular *** are the implications for public health practice?The experience of prevention and control measures during the coronavirus disease 2019(COVID-19)pandemic has provided a reference for the prevention and control of other infectious diseases through selfsampling.

关键词： prevention acute respiratory

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Impact factors of Blastocystis hominis infection in persons living with human immunodeficiency virus:a large-scale,multi-center observational study from China

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infectious diseases of Poverty 2023年第5期12卷 95-96页

作者： Shun-Xian Zhang Ji-Chun Wang Zhong-Wei Li Jin-Xin Zheng Wen-Ting Zhou Guo-Bing Yang Ying-Fang Yu Xiu-Ping Wu Shan Lv Qin Liu Mu-Xin Chen Yan Lu Zhi-Hui Dou Da-Wei Zhang Wen-Wen Lv Lei Wang Zhen-Hui Lu Ming Yang Pei-Yong Zheng Yue-Lai Chen Li-Guang Tian Xiao-Nong Zhou Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai China Chinese center for disease Control and prevention (Chinese center for Tropical diseases research) NHC Key Laboratory of Parasite and Vector Biology WHO Collaborating Centre for Tropical Diseases National Center for International Research On Tropical Diseases National Institute of Parasitic Diseases Shanghai China School of global Health Chinese Center for Tropical Diseases Research-Shanghai Jiao Tong University School of Medicine Shanghai China Department of Science and Technology Chinese Center for Disease Control and Prevention Beijing China Gansu Province People’s Hospital Gansu Provincial Hospital Lanzhou China National Health Commission (NHC) Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China Gansu Provincial center for disease Control and prevention Lanzhou China National center for AIDS/STD Control and prevention China Center for Disease Control and Prevention Beijing China The People’s Liberation Army 302 Hospital Beijing China Clinical research Institute Shanghai Jiao Tong University School of Medicine Shanghai China

Background Blastocystis hominis(Bh)is zoonotic parasitic pathogen with a high prevalent globally,causing opportunistic infections and diarrhea *** immunodeficiency virus(HIV)infection disrupts the immune system by depleting CD4^(+)T lymphocyte(CD4^(+)T)cell counts,thereby increasing Bh infection risk among persons living with HIV(PLWH).However,the precise association between Bh infection risk and HIV-related biological markers and treatment processes remains poorly ***,the purpose of the study was to explore the association between Bh infection risk and CD4^(+)T cell counts,HIV viral load(VL),and duration of interruption in antiviral therapy among *** A large-scale multi-center cross-sectional study was conducted in China from June 2020 to December *** genetic presence of Bh in fecal samples was detected by real-time fluorescence quantitative polymerase chain reaction,the CD4^(+)T cell counts in venous blood was measured using flowcytometry,and the HIV VL in serum was quantified using fluorescence-based *** cubic spline(RCS)was applied to assess the non-linear association between Bh infection risk and CD4^(+)T cell counts,HIV VL,and duration of interruption in highly active antiretroviral therapy(HARRT).Results A total of 1245 PLWH were enrolled in the study,the average age of PLWH was 43 years[interquartile range(IQR):33,52],with 452(36.3%)being female,50.4%(n=628)had no immunosuppression(CD4^(+)T cell counts>500 cells/μl),and 78.1%(n=972)achieved full virological suppression(HIV VL<50 copies/ml).Approximately 10.5%(n=131)of PLWH had *** prevalence of Bh was found to be 4.9%[95%confidence interval(CI):3.8-6.4%]among *** nonlinear associations were observed between the Bh infection risk and CD4^(+)T cell counts(Pfor nonlinearity<0.001,L-shaped),HIV VL(Pfor nonlinearity<0.001,inverted U-shaped),and duration of interruption in HARRT(Pfor nonlinearity<0.001,inverted U-shaped).Conclusions

关键词： Blastocystis hominis Human immunodeficiency virus Viral load Non-linear association Restricted cubic spline Propensity score China

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