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Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

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Journal of Integrative Agriculture 2016年第1期15卷 120-134页

作者： LI Yu WANG Ke XIE Hui XU Chun-ling WANG Dong-wei LI Jing HUANG Xin PENG Xiao-fang Laboratory of plant Nematology and research center of nematodes of plant quarantine Department of Plant Pathology South China Agricultural University

Ten populations of Radopholus similis from different ornamental hosts were tested for their parasitism and pathogenicity to water spinach （Ipomoea aquatic）, malabar spinach （Basella rubra）, and squash （Cucurbita moschata） in pots. The re- sults showed all three plants were new hosts of R. similis. Growth parameters of plants inoculated with nematodes were significantly lower than those of healthy control plants. All R. similis populations were pathogenic to the three plants, but pathogenicity differed among populations from different hosts. The same R. similis populations also showed different pathogenic effects in the three different plants. RadN5 population from Anthurium andraeanum had the highest pathoge- nicity to the three studied plants. RadN1 from A. andraeanum had the lowest pathogenicity to squash and RadN7 from Chrysalidocarpus lutesens had the lowest pathogenicity to water spinach and malabar spinach. R. similis is usually associated with root tissues, but here we report that it could be found to move and feed in the stem bases of all three studied plants. Sequence and phylogenetic analyses of DNA markers of the 18S rRNA, 28S rRNA, ITS rRNA, and mitochondrial DNA gene sequences of ten R. similis populations revealed significant genetic diversity. RadN5 and RadN6 populations from anthurium showed a close genetic relationship and could be distinguished from other populations by PCR-RFLP. At the same time, RadN5 and RadN6 populations were the most pathogenic to three studied plants. These results confirm the existence of large biological variability and molecular diversity among R. similis populations from the same or different hosts, and these characteristics are related to pathogenic variability.

关键词： burrowing nematode parasitism pathogenicity genetic diversity PCR-RFLP

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A chorismate mutase from Radopholus similis plays an essential role in pathogenicity

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Journal of Integrative Agriculture 2024年第3期23卷 923-937页

作者： Sihua Yang Junyi Li Shuai Yang Shiqiao Tang Huizhong Wang Chunling Xu Hui Xie Laboratory of plant Nematology andresearch centerof nematodes of plant quarantine Department of Plant Patholo of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China

In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study,based on transcriptome data, a chorismate mutase gene of Radopholus similis(RsCM) was identified and cloned,which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity(PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference(RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid(SA) pathway genes(PR1 and PR5) in roots infected by the nematode were significantly down-regulated,which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.

关键词： Radopholus similis chorismate mutase plant defense transgenic tomato

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Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host

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Journal of Integrative Agriculture 2022年第9期21卷 2652-2664页

作者： YANG Si-hua ZHAO Li-rong DING Sha TANG Shi-qiao CHEN Chun ZHANG Huan-xin XU Chun-ling XIE Hui research center of nematodes of plant quarantine Laboratory of Plant NematologyCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642P.R.China Technology center Guangzhou Customs DistrictPeople's Republic of ChinaGuangzhou 510623P.R.China Comprehensive Technology and Service center of Huizhou Port Customs Huizhou 516080P.R.China

Radopholus similis(Cobb 1893) Thorne(1949) is a destructive migratory endoparasitic plant nematode. In this study,the pathogenic process of R. similis infection in Nicotiana benthamiana(tobacco) was studied using quartz sand culture in laboratory. The results showed that R. similis mainly parasitised the root cortex, leading to cortical cell decomposition and tissue decay. We optimised the inoculation conditions to establish a method for determining the pathogenicity of R. similis as follows:(1) a glass culture tube was filled with quartz sand(about 1/3 of the height) and sterilised twice;(2) 20-day-old N. benthamiana seedlings were transplanted into test tubes and cultivated for 10 days at(25±1)°C;(3) R. similis female nematodes were inoculated in the root rhizosphere at a rate of 150 nematodes per plant;(4) the number of nematodes, disease severity, and growth of the plant at 30 days post-inoculation(dpi) were determined. The pathogenicity of eight R. similis populations from different hosts was determined, which proved the feasibility of this method.

关键词： Radopholus similis Nicotiana benthamiana populations pathogenicity

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Analysis of metabolites of coix seed fermented by Monascus purpureus

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Food Bioscience 2022年第6期50卷 546-554页

作者： Qi Mei Zhaona Xu Quanye Wu Likang Qin Haiying Zeng Yi Zhu School of Liquor and Food Engineering Guizhou UniversityGuiyang550025China plant Protection and plant quarantine Station Guizhou ProvinceGuiyang550025China

In this study,an untargeted metabolomics method based on LC-MS was used to investigate the differential metabolites of coix seed after fermentation by Monascus *** compounds with highly correlated variable importance in projection(VIP)values of orthogonal partial least squares discriminant analysis(OPLS-DA)were selected as the important metabolites of coix seed *** seed was fermented by *** for 9 *** color value units(CVU)of pigment in fermented coix seed reached 157.47 CVU/g,and the contents of total phenols and total flavonoids were 0.32 mg/g and 1.2 mg/g,respectively.A total of 270 differential metabolites were detected,including amino acids,peptides,fatty acids,phenols,steroids,and their derivatives,of which the relative contents of 187 metabolites showed a significant upward trend(p<0.05).Amino acids,peptides and analogues accounted for the largest proportion at 26%,among which most metabolites were *** example,N-alpha-acetyllysine,l-tryptophan and l-arginine exhibited a fold change(FC)of 6200.87,330.29,and 35.59,*** particular,the detected steroid metabolites accounted for 3%of the total metabolites,and they all participated in the same pathway,in which androsterone,2-methoxyestrone and etiocholanolone were *** synthesis of these steroids was related to the precursors and enzymes in the pathway,such as dehydroepiandrosterone,androstenedione,and 3beta-hydroxy-delta5-steroid dehydrogenase(HSD3B).The results of this study reveal *** fermentation can successfully transform coix seed,improving the functional activities of ***,this process has high development value with important applications in the food industry.

关键词： Fermented coix seed Monascus purpureus Untargeted metabolomics Metabolites

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Cas9-targeted Nanopore sequencing rapidly elucidates the transposition preferences and DNA methylation profiles of mobile elements in plants

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Journal of Integrative plant Biology 2023年第10期65卷 2242-2261页

作者： Pavel Merkulov Sofya Gvaramiya Maxim Dudnikov Roman Komakhin Murad Omarov Alina Kocheshkova Zakhar Konstantinov Alexander Soloviev Gennady Karlov Mikhail Divashuk Ilya Kirov All‐Russia research Institute of Agricultural Biotechnology Moscow 127550Russia Moscow Institute of Physics and Technology Dolgoprudny 141701Russia All‐Russia center for plant quarantine Ramenski 140150Russia N.V.Tsitsin Main Botanical Garden of the Russian Academy of Sciences Moscow 127276Russia

Transposable element insertions(TEIs) are an important source of genomic innovation by contributing to plant adaptation, speciation, and the production of new varieties. The often large,complex plant genomes make identifying TEIs from short reads difficult and expensive. Moreover, rare somatic insertions that refect mobilome dynamics are difficult to track using short *** address these challenges, we combined Cas9-targeted Nanopore sequencing(CANS) with the novel pipeline Nano Cas TE to trace both genetically inherited and somatic TEIs in plants. We performed CANS of the EVADé(EVD) retrotransposon in wild-type Arabidopsis thaliana and rapidly obtained up to 40× sequence *** of hemizygous T-DNA insertion sites and genetically inherited insertions of the EVD transposon in the ddm1(decrease in DNA methylation1) genome uncovered the crucial role of DNA methylation in shaping EVD insertion *** also investigated somatic transposition events of the ONSEN transposon family, finding that genes that are downregulated during heat stress are preferentially targeted by ONSENs. Finally, we detected hypomethylation of novel somatic insertions for two ONSENs. CANS and Nano Cas TE are effective tools for detecting TEIs and exploring mobilome organization in plants in response to stress and in different genetic backgrounds, as well as screening T-DNA insertion mutants and transgenic plants.

关键词： Arabidopsis DNA methylation Nanopore sequencing transposon insertions

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Predicting the potential geographic distribution of Bactrocera bryoniae and Bactrocera neohumeralis(Diptera: Tephritidae) in China using MaxEnt ecological niche modeling

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Journal of Integrative Agriculture 2020年第8期19卷 2072-2082页

作者： Jing Wan QI Guo-jun MA Jun Yonglin REN WANG Rui Simon MCKIRDY Harry Butler Institute Murdoch UniversityPerthWA 6150Australia Guangdong Provincial Key Laboratory of High Technology for plant Protection Plant Protection Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640P.R.China Guangdong Inspection and quarantine Technology center Guangzhou 510623P.R.China Institute of plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

Bactrocera bryoniae and Bactrocera neohumeralis are highly destructive and major biosecurity/quarantine pests of fruit and vegetable in the tropical and subtropical regions in the South Pacific and *** these pests have not established in China,precautions must be taken due to their highly destructive ***,we predicted the potential geographic distribution of *** and *** across the world and in particular China by ecological niche modeling of the Maximum Entropy(Max Ent)model with the occurrence records of these two *** bryoniae and *** exhibit similar potential geographic distribution ranges across the world and in China,and each species was predicted to be able to distribute to over 20%of the ***,the potential geographic distribution ranges for these two fruit fly species included southern Asia,the central and the southeast coast of Africa,southern North America,northern and central South America,and *** within China,most of the southern Yangtze River area was found suitable for these ***,southern China was considered to have the highest risk of *** and *** *** study identifies the regions at high risk for potential establishment of *** and *** in the world and in particular China,and informs the development of inspection and biosecurity/quarantine measures to prevent and control their invasions.

关键词： fruit fly Bactrocera bryoniae Bactrocera neohumeralis ecological niche modeling MaxEnt potential geographic distribution habitat suitability

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Chrysotus Meigen(Diptera: Dolichopodidae) from Shanxi, China, with descriptions of two new species

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Zoological Systematics 2015年第1期40卷 86-92页

作者： Ruo-Si Liu Meng-Qing Wang Ding Yang Department of Entomology China Agricultural University Beijing Entry-Exit Inspection and quarantine Bureau Technology center Institute of plant Protection Chinese Academy of Agricultural Sciences

The species of the genus Chrysotus Meigen from Shanxi, China are reviewed, Two species are described as new to science： C. shanxiensis sp. nov. and C. thornpenis sp. nov. C. biprojicienus Wei ＆ Zhang and C. serratus Wang ＆ Yang are recorded from Shanxi for the first time. A key to the species of Chrysotus from Shanxi is presented.

关键词： Diptera Dolichopodidae Diaphorinae Chrysotus Shanxi new species.

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Screening of DNA Barcode of quarantine Phytophthora

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Agricultural Biotechnology 2017年第1期6卷 46-51,54页

作者： Ruifang GAO Yinghui CHENG Ying WANG Ying WANG Liyun GUO Guiming ZHANG Animal and plant Inspection and quarantine Technology center Shenzhen Entry-Exit Inspection and Quarantine Bureau Key Laboratory of Exotic Pest Detection Technology research of Shenzhen College of Agriculture and Biotechnology China Agricultural University

Phytophthora is genus of plant-damaging Oomycetes, whose member species are capable of causing enormous economic losses on crops worldwide. In the present study, four candidate genes ITS, CO1, EF-1α and β-tubulin were tested using 123 strains of 80 species of Phytophthora to investigate the feasibility of ser- ving as DNA barcoding markers. The results showed that among the four candidate genes, ITS and CO1 had the highest success rate of PCR amplification and se- quencing, up to 100% and 96.7%. There were obvious barcode gaps in ITS, CO1 andβ-tubulin, but their frequency distributions of intra- and interspecific genetic distances were slightly overlapped. Wilcoxon rank sum test on intraspecific genetic distances of the four genes showed ITS = CO1 = β - tubulin = EF-1α indicating they bad the same effect on intraspecific discrimination, while the test on interspecific genetic distances of the four genes showed ITS 〉 C01 〉 β- tubulin 〉 EF - 1α. In summary, ITS and COl should be used in combination as the primary barcodes, β-tubulin as the complementary barcede for the identification of 11 quaran- tine Phytophthora species.

关键词： quarantine Phytophthora DNA barcoding Genetic distance Barcoding gap

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Neonatal lethality and recycling defect of transferrin receptor in mice with Syntaxin12/13 disruption

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Protein & Cell 2019年第1期10卷 67-71页

作者： Fang Li Chun-Feng Liu Yin-Zhen Xu Yi-Lin Guo Shu-Wen Xue Xiang-Dong Kong Hai-bing Zhang Yan Zhang Jian-Sheng Kang CAS Key Laboratory of Nutrition and Metabolism Institute for Nutritional SciencesShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052China Technical center for Animal Plant and Food Inspection and QuarantineShanghai Entry-Exit Inspection and Quarantine BureauShanghai 20013China

Dear Editor,Iron deficiency,documented by World Health Organization(WHO),is the most common nutritional deficiency,and accounts for-50%of anemia ***-deficiency anemia is notably and frequently associated with chronic heart failure,chronic kidney disease,cancer and inflammatory bowel *** to WHO Global Health Estimates 2014 Summary,iron-deficiency anemia is a major and prevalent public health problem worldwide,which contributes to 0.2% mortality,especially maternal and child mortality.

关键词： Editor World mortality

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Rapid detection of potato late blight using a loop-mediated isothermal amplification assay

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Journal of Integrative Agriculture 2020年第5期19卷 1274-1282页

作者： KONG Liang WANG Hui-bin WANG Shuai-shuai XU Ping-ping ZHANG Ruo-fang DONG Suo-meng ZHENG Xiao-bo Department of plant Pathology Nanjing Agricultural UniversityNanjing 210095P.R.China Nanjing Exit and Entry Inspection and quarantine Bureau Nanjing 210001P.R.China Potato research center Inner Mongolia UniversityHohhot 010021P.R.China The Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of EducationNanjing 210095P.R.China

Potato late blight caused by Phytophthora infestans is one of the most destructive plant diseases that threaten global food security. Early and effective diagnosis of P. infestans is required before disease management decisions are made. Here, we developed a quick protocol to detect P. infestans based on a loop-mediated isothermal amplification(LAMP) assay. The P. infestans specific multiple copy DNA sequences(PiSMC), a transposon-like element, provides an ideal target for molecular detection of this pathogen. We designed highly specific and sensitive primers allowing effective LAMP detection of the pathogen at 64℃ in 70 min. In the validation assay, all 15 P. infestans isolates collected from China, Europe and South America could be positively detected, but results of the other 9 Phytophthora species infecting different plants, fungal and bacterial plant pathogens tested were negative. The detection limit of this assay is 1 pg P. infestans DNA. Moreover, the LAMP-PiSMC assay is able to detect P. infestans from infected leaves, tubers and soil. Taken together, this study reports the development of a specific and sensitive LAMP-PiSMC assay for early diagnosis of potato late blight.

关键词： Phytophthora infestans potato late disease LAMP assay diagnostic detection

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