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Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome

World Journal of Gastroenterology 2010年第36期16卷 4532-4540页

作者： Erja Malinen Lotta Krogius-Kurikka Janne Nikkil Airi Palva Anna Lyra Anne Jskelinen Teemu Rinttil Terttu Vilpponen-Salmela Atte Johannes von Wright Department of Veterinary Biosciences Faculty of Veterinary MedicineUniversity of Helsinki00014 HelsinkiFinland Danisco Sweeteners Health and Nutrition02460 KantvikFinland Department of Clinical nutrition Institute of Public Health and Clinical NutritionFaculty of Health SciencesUniversity of Eastern Finland70211 KuopioFinland Alimetrics ltd. Koskelontie 19 B02920 EspooFinland Kuopio Harjula Hospital 70101 KuopioFinland Department of Biosciences University of Eastern Finland70211 KuopioFinland

AIM:To investigate the correlations between selfreported symptoms of irritable bowel syndrome(IBS) and the gastrointestinal(GI) microbiota ***:Fecal samples were collected from a total of 44 subjects diagnosed with *** symptoms were monitored with a validated inflammatory bowel disease questionnaire adjusted for IBS *** quantitative real-time polymerase chain reaction assays were applied to evaluate the GI microbiota *** and GI bacterial genera(Bifidobacterium,Lactobacillus and Veillonella),groups(Clostridium coccoides/Eubacterium rectale,Desulfovibrio desulfuricans) and distinct bacterial phylotypes [closest 16S rDNA sequence resemblance to species Bifidobacterium catenulatum,Clostridium cocleatum,Collinsella aerofaciens(***),Coprococcus eutactus(***),Ruminococcus torques and Streptococcus bovis ] with a suspected association with IBS were *** between quantities or presence/absence data of selected bacterial groups or phylotypes and various IBSrelated symptoms were ***:Associations were observed between subjects' self-reported symptoms and the presence or quantities of certain GI bacteria.A Ruminococcus torques(***)-like(94% similarity in 16S rRNA gene sequence) phylotype was associated with severity of bowel ***,among IBS subjects with *** 94% detected,the amounts of *** 88%,***-like and *** 97% phylotypes were significantly *** observations were also made concerning the effect of a subject's weight on GI microbiota with regard to *** like phylotype,Bifidobacterium *** Lactobacillus ***:Bacteria seemingly affecting the symptom scores are unlikely to be the underlying cause or cure of IBS,but they may serve as biomarkers of the condition.

关键词： Irritable bowel syndrome Self-reported symptoms Gastrointestinal microbiota Quantitative realtime polymerase chain reaction

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Implications of vitamin D for flesh quality of grass carp(Ctenopharyngodon idella):antioxidant ability,nutritional value,sensory quality,and myofiber characteristics

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2596-2613页

作者： Yao Zhang Chaonan Li Xiaoqiu Zhou Weidan Jiang Pei Wu Yang Liu Hongmei Ren Lu Zhang Haifeng Mi Jiayong Tang Ruinan Zhang Lin Feng Animal nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina. Fish nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China. Key Laboratory for Animal Disease‑Resistant nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 611130China. Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.Chengdu 610041SichuanChina.

Background Muscle represents a unique and complex system with many components and comprises the major edible part of *** D is a critical nutrient for animals and is known to enhance calcium absorption and immune *** recent years,dietary vitamin D supplementation in livestock has received increased attention due to biological responses including improving shear force in mammalian ***,the vitamin D acquisition and myofiber development processes in fish differ from those in mammals,and the effect of vitamin D on fish flesh quality is poorly ***,the influence of dietary vitamin D on fillet quality,antioxidant ability,and myofiber development was examined in grass carp(Ctenopharyngodon idella).Methods A total of 540 healthy grass carp,with an initial average body weight of 257.24±0.63 g,were allotted in 6 experimental groups with 3 replicates each,and respectively fed corresponding diets with 15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg vitamin D for 70 *** Supplementation with 1,167.9 IU/kg vitamin D significantly improved nutritional value and sensory quality of fillets,enhancing crude protein,free amino acid,lipid,and collagen contents;maintaining an ideal pH;and reduc-ing lactate content,shear force,and cooking loss relative to respective values in the control(15.2 IU/kg)*** myofiber diameter and the frequency of myofibers>50μm in diameter increased under supplementation with 782.5–1,167.9 IU/kg vitamin *** of oxidative damage biomarkers decreased,and the expression of antioxi-dant enzymes and nuclear factor erythroid 2-related factor 2 signaling molecules was upregulated in the 1,167.9 IU/kg vitamin D treatment compared to respective values in the control ***,vitamin D supplementation activated cell differentiation by enhancing the expression of myogenic regulatory factors and myocyte enhancer factors compared to that in the control *** addition,supplementation with 1,167.9 IU/kg

关键词： Antioxidant Grass carp Myofiber development nutritional value Sensory quality Vitamin D

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Accuracy of predicting metabolizable energy from in vitro digestible energy determined with a computer-controlled simulated digestion system in feed ingredients for ducks

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Animal nutrition 2022年第1期8卷 52-60页

作者： Yuming Wang Liting Yin Hu Zhang Ke Li Dailin Li Feng Zhao The State Key Laboratory of Animal nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Newhope Liuhe Co.ltd. Beijing 100102China

Two experiments were conducted to study the accuracy of predicting true metabolizable energy(TME)of ingredients for ducks from in vitro digestible energy(IVDE)determined with a computer-controlled simulated digestion *** 1 was to establish TME prediction models from the IVDE of 9 energy feed ingredients and 12 protein feed ingredients using regression *** 2 was to validate the accuracy of the predicted ME of 10 ingredients randomly selected from *** diets were formulated with 2 to 6 of 10 *** in vivo TME values were compared with calculated values based on the TME predicted in *** Exp.1,the correlation coefficients between TME and IVDE were 0.9339(P<0.05)in 9 energy feed ingredients and 0.8332(P<0.05)in 12 protein feed *** significant difference was observed on the slope and intercept of TME regression models between 9 energy feed ingredients and 12 protein feed ***,the regression model of TME on IVDE for 21 feed ingredients was TME=0.7169×IVDE+1,224(R^(2)=0.7542,P<0.01).Determined and predicted TME differed by less than 100 kcal/kg of DM in 11 ingredients,and the difference ranged from 100 to 200 kcal/kg of DM in 5 ***,the difference between determined and predicted TME varied from 410 to 625 kcal/kg of DM in rice bran,rapeseed meal,corn gluten meal,and citric acid *** Exp.2,the determined and calculated TME were comparable(3,631 vs.3,639 kcal/kg of DM)and highly correlated(r=0.9014;P<0.05)in 10 *** and calculated TME differed by less than 100 kcal/kg of DM in 7 diets and by 106 to 133 kcal/kg of DM in 3 *** results have demonstrated that TME can be accurately predicted from IVDE in most feed ingredients,but it is less accurate for rice bran,rapeseed meal,corn gluten and citric acid meal.

关键词： Duck In vitro digestible energy Prediction model True metabolizable energy

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Dietary supplementation of 25-hydroxycholecalciferol increases tibial mass by suppression bone resorption in meat ducks

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Animal nutrition 2020年第4期6卷 467-479页

作者： Huaiyong Zhang Qiufeng Zeng Shiping Bai Jianping Wang Xuemei Ding Yue Xuan Zhuowei Su Gergory S.Fraley Bing Yao Keying Zhang Institute of Animal nutrition Key Laboratory for Animal Disease-Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural UniversityChengduSichuan611130China Biology Department Hope CollegeHolland49423USA DSM(China)ltd. PuDong New AreaShanghai201203China

Leg problems often result from the rapid weight gain and poor bone quality in modern ducks,leading to a high risk of fractures and continuous *** hypothesized that improving bone quality in combination with delaying weight gain via a low nutrient density(LND)diet probably reverses these skeletal *** indicated that 25-hydroxycholecalciferol(25-OH-D3),a vitamin D3 metabolite,is effective in treating bone-related ***,Exp.1 evaluated the effects of 25-OH-D3 on tibial mass of meat *** meat ducklings were fed a standard nutrient density diet(containing a regular vitamin regimen)without or with 25-OH-D3 at 0.069 mg/kg for 35 *** results showed that 25-OH-D3 supplementation improved the mineral content,microarchitecture and mechanical properties of tibias,and this companied by a decreased serum bone resorption marker and a concomitant decrement in osteoclast-specific marker genes ***,Exp.2 was conducted to examine the impacts of 25-OH-D3 incorporating an LND diet on tibial quality of ducks under 2 different vitamin regimens(regular and high).Ducklings were allocated to a 2*2 factorial arrangement with 2 kinds of vitamin premixes and without or with 25-OH-D3 at 0.069 mg/kg in LND *** high premix had higher levels of all vitamins except biotin than the regular *** results demonstrated that high vitamin diets exhibited more significant effects than regular vitamin diets on inhibiting bone turnover and increasing minerals *** mineral content,microarchitecture,and strength of birds under the regular vitamin regimen were increased by 25-OH-D3 supplementation;However,these positive effects were not observed in ducks under the high vitamin *** conclude,25-OH-D3 supplementation improves tibial mass by suppressing osteoclast-mediated bone resorption in meat ducks,and this positive impact only was observed in regular but not high vitamin regimen when birds fed an LND diet.

关键词： Vitamin D_(3)metabolites Vitamin Bone turnover Tibial mass Meat duck

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A new insight on copper:Promotion of collagen synthesis and myofiber growth and development in juvenile grass carp(Ctenopharyngodon idella)

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Animal nutrition 2023年第4期15卷 22-33页

作者： Rui Ma Lin Feng Pei Wu Yang Liu Hong-Mei Ren Shu-Wei Li Ling Tang Cheng-Bo Zhong Dong Han Wen-Bing Zhang Jia-Yong Tang Xiao-Qiu Zhou Wei-Dan Jiang Animal nutrition Institute Sichuan Agricultural UniversityChengdu611130China Fish nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu611130China Key Laboratory of Animal Disease-Resistance nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan611130China Animal nutrition Institute Sichuan Academy of Animal ScienceSichuan Animtech Feed Co.LtdChengdu610066SichuanChina State Key Laboratory of Fresh Water Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan430072China The Key Laboratory of Mariculture Ministry of EducationThe Key Laboratory of Aquaculture Nutrition and FeedsMinistry of AgricultureOcean University of ChinaQingdao266003China

Copper(Cu)is a trace element,essential for fish *** the current study,in addition to growth performance,we first explored the effects of Cu on collagen synthesis and myofiber growth and development in juvenile grass carp(Ctenopharyngodon idella).A total of 1080 fish(11.16±0.01 g)were randomly divided into 6 treatments(3 replicates per treatment)to receive five doses of organic Cu which were Cu citrate(CuCit)at 0.99(basal diet),2.19,4.06,6.15,and 8.07 mg/kg,and one dose o inorganic Cu(CuSO_(4)·5H_(2)O at 3.15 mg/kg),for 9 *** results showed appropriate Cu level(4.06 mg kg)enhanced growth performance,improved nutritional Cu status,and downregulated Cu-transporting ATPase 1 mRNA levels in the hepatopancreas,intestine,and muscle of juvenile grass *** collagen content in fish muscle was increased after Cu intake,which was probably due to the following pathways:(1)activating CTGF/TGF-β1/Smads signaling pathway to regulate collagen transcription;(2upregulating of La ribonucleoprotein domain family 6(LARP6)mRNA levels to regulate translation initiation;(3)increasing proline hydroxylase,lysine hydroxylase,and lysine oxidase activities to regulate posttranslational *** addition,optimal Cu group increased myofiber diameters and the frequency of myofibers with diameter>50μm,which might be associated with upregulation of cyclin B cyclin D,cyclin E,proliferating cell nuclear antigen,myogenic determining factor(MyoD),myogenic factor 5,myogenin(MyoG),myogenic regulatory factor 4 and myosin heavy chain(MyHC)and down regulation of myostatin mRNA levels,increasing protein levels of MyoD,MyoG and MyHC in fish muscle Finally,based on percentage weight gain(PWG),serum ceruloplasmin(Cp)activity and collagen conten in fish muscle,Cu requirements were determined as 4.74,4.37 and 4.62 mg/kg diet(CuCit as Cu source of juvenile grass carp,*** on PWG and Cp activity,compared to CuSO_(4)·5H_(2)O,the efficacy of CuCit were 131.80%and 115.38%,resp

关键词： Copper Grass carp(Ctenopharyngodon idella) Collagen synthesis Myofiber

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Dietary β-glucan supplementation improves growth performance,carcass traits and meat quality of finishing pigs

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Animal nutrition 2019年第4期5卷 380-385页

作者： Junqiu Luo Dafu Zeng Long Cheng Xiangbing Mao Jie Yu Bing Yu Daiwen Chen Institute of Animal nutrition Sichuan Agricultural UniversityKey Laboratory of AnimalDisease Resistance NutritionMinistry of EducationChengdu 611130China Sichuan Synlight Biotech ltd. Chengdu 610041China Faculty of Veterinary and Agricultural Sciences Dookie Campus 3647The University of MelbourneParkvilleVIC3052Australia

This experiment was conducted to investigate growth performance,carcass traits and meat quality of finishing pigs with dietary β-glucan supplementation.A total of 96 healthy pigs(Duroc x Landrace x Yorkshire;initial average BW=25 kg) were randomly allocated into 4 dietary treatments with 6 replicates per treatment and 4 pigs per *** control group was fed a basal diet,and the experimental diets were supplemented with 50,100 and 200 mg/kg Agrobacterium ***09β-glucan,*** experiment lasted 103 *** basal diet supplemented with 100 mg/kg β-glucan significantly increased average daily gain and feed conversion ratio,probably due to the improved digestibility of dry matter,gross energy and crude protein(P <0.05).Beta-glucan supplementation from100 to 200 mg/kg of diet significantly increased carcass length(P <0.05).The basal diet supplemented with 100 mg/kg β-glucan supplementation also significantly(P <0.05) increased muscle pH,reduced drip losses and increased a~**** basal diet supplemented with 100 mg/kg β-glucan increased the content of intramuscular fat and changed the proportion of saturated fatty acid and unsaturated fatty acid,thereby improved the flavor of *** conclusion,the basal diet supplemented with 100 mg/kg Agrobacterium ***09 β-glucan improves growth performance,nutrient digestibility,carcass length,and pork quality of finishing pigs.

关键词： Growth performance β-glucan Carcass traits Meat quality Finishing pigs

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Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou Animal nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei Co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment consisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic Ochratoxin A Protein deposition

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The role of vitamin E in polyunsaturated fatty acid synthesis and alleviating endoplasmic reticulum stress in sub-adult grass carp(Ctenopharyngodon idella)

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Animal nutrition 2024年第1期16卷 275-287页

作者： Ke Yao Lin Feng Wei-Dan Jiang Yang Liu Lu Zhang Hai-Feng Mi Xiao-Qiu Zhou Pei Wu Animal nutrition Institute Sichuan Agricultural UniversityChengdu611130China Fish nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu611130China Key Laboratory of Animal Disease-Resistance nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu611130China Tongwei Co. Ltd.Chengdu610041China Healthy Aquaculture Key Laboratory of Sichuan Province Chengdu610041China

Vitamin E(VE)is an essential lipid-soluble vitamin that improves the fish flesh ***,the underlying molecular mechanisms remain *** study aimed to investigate the effects of VE on growth performance and flesh quality in sub-adult grass carp(Ctenopharyngodon idella).A total of 450fish(713.53±1.50 g)were randomly divided into six treatment groups(three replicates per treatment)and fed for nine weeks with different experimental diets(dietary lipid 47.8 g/kg)that contained different levels of VE(5.44,52.07,96.85,141.71,185.66,and 230.12 mg/kg diet,supplemented as dl-a-tocopherol acetate).Notably,the treatment groups that were fed with dietary VE ranging from 52.07 to230.12 mg/kg diet showed improvement in the percent weight gain,special growth rate,and feed efficiency of grass ***,the treatment groups supplemented with dietary VE level of 141.71,185.66,and 230.12 mg/kg diet showed enhancement in crude protein,lipid,and a-tocopherol contents in the muscle,and the dietary levels of VE ranging from 52.07 to 141.71 mg/kg diet improved muscle pH24h and shear force but reduced muscle cooking loss in grass ***,appropriate levels of VE(52.07 to 96.85 mg/kg diet)increased the muscle polyunsaturated fatty acid content in grass *** VE also increased the mRNA levels of fatty acid synthesis-related genes,including fas,scd-1,fad,elovl,srebp1,pparγ,and lxra,and up-regulated the expression of SREBP-1 ***,dietary VE decreased the expression of fatty acid decomposition-related genes,including hsl,cpt1,acox1,and ppara,and endoplasmic reticulum stress-related genes,including perk,ire1,atf6,eif2a,atf4,xbp1,chop,and grp78,and down-regulated the expression of p-PERK,p-IRE1,ATF6,and GRP78 *** conclusion,dietary VE increased muscle fatty acid synthesis,which may be partly associated with the alleviation of endoplasmic reticulum stress,and ultimately improves fish flesh ***,the VE requirements for sub-adult gras

关键词： Vitamin E Flesh quality Muscle polyunsaturated fatty acid level Endoplasmic reticulum stress Growth performance Grass carp

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An automatically progressed computer-controlled simulated digestion system to predict digestible and metabolizable energy of unconventional plant protein meals for growing pigs

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Animal nutrition 2022年第3期10卷 178-187页

作者： Zhongyuan Du Yuming Wang Mingqiang Song Shuli Zeng Lixiang Gao Jiangtao Zhao Feng Zhao State Key Laboratory of Animal nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing100193China Wen's Food Group Co.ltd. Guangdong527439China

The objective of this experimentwas to develop a new computer-controlled simulated digestion system to predict the digestible energy(DE)and metabolizable energy(ME)of unconventional plant protein meals for growing *** meals tested included 1 source of rapeseed meal,4 sources of cottonseed meal,2 sources of sunflower meal,and 2 sources of peanut *** growing pigs(Duroc[LandraceLarge White])with an initial body weight(BW)of 41.7±2.6 kg were allotted to a replicated 103 incomplete Latin square design to determine the DE and ME of 1 basal diet and 9 experimental diets formulated with 9 unconventional plant protein *** DE andMEvalues of unconventional plant protein meals were calculated by the difference *** in vitro digestible energy(IVDE)of 1 basal diet,9 experimental diets,and 9 unconventional plant protein meals were determined with 5 replicates of each sample in a complete randomized *** IVDE/DE or IVDE/ME ranged from 0.96 to 0.98 or 1.00 to 1.01,and the correlation coefficient between IVDE and DE or MEwas 0.97 or 0.98 in 10 experimental ***,the IVDE/DE or IVDE/ME ranged from 0.86 to 1.05 or 0.96 to 1.20,and the correlation coefficient between IVDE and DE orME was 0.92 or 0.91 in 9 unconventional plant protein *** coefficient of variation(CV)of IVDE was less than that of DE and ME in the experimental diets(0.43%,0.80%,and 0.97%for CV of IVDE,DE and ME,respectively)and unconventional plant protein meals(0.92%,4.84%,and 6.33%for CV of IVDE,DE and ME,respectively).The regression equations to predict DE from IVDE in 10 experimental diets and 9 unconventional plant protein meals were DE=0.8851IVDE t539(R^(2)=0.9411,residual standard deviation[RSD]=23 kcal/kg DM,P<0.01)and DE=0.9880IVDE t 166(R^(2)=0.8428,RSD=182 kcal/kg DM,P<0.01),*** no statistical difference in the slopes(P=0.82)or intercepts(P=1.00)of these 2 ***,10 diets and 9 unconventional plant protein meals were pooled to

关键词： Digestible energy In vitro digestible energy Metabolizable energy Unconventional plant protein meal Pig Simulated digestion system

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Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets

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Journal of Animal Science and Biotechnology 2021年第2期12卷 700-710页

作者： Jie Yu Yanyan Song Bing Yu Jun He Ping Zheng Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Quyuan Wang Huifen Wang Daiwen Chen Animal nutrition Institute Sichuan Agricultural UniversityChengdu 611130People’s Republic of China Key Laboratory for Animal Disease-Resistance nutrition Ministry of Education of ChinaChengdu 611130People’s Republic of China

Background:Tannic acid(TA)is potential to reduce diarrhea in weaning pigs,but knowledge about the influence of TA on intestinal barrier integrity and function is still *** experiment was conducted to investigate the effects of dietary TA supplementation on growth performance,diarrhea rate,intestinal barrier integrity and function of weaned ***:A total of 108 crossbred(Duroc×Landrace×Yorkshire)piglets,with an initial average body weight of 6.60±0.27 kg,were allotted to 3 groups(6 pigs/pen and 6 replicates/group)in a randomized complete block design according to their gender and body *** were fed the basal diet with 0(control,CON),0.2%and 1.0%TA,*** trial lasted for 28 ***:Compared with the CON group,dietary 0.2%and 1.0%TA supplementation didn’t affect ADFI,ADG and F:G(P>0.05),but reduced diarrhea rate,diarrhea index and diarrhea score of piglets(P<0.05),reduced diamine oxidase(DAO)activity and D-lactic acid concentration in serum(P<0.01).The higher occludin expression and localization were observed in the duodenum,jejunum and ileum after supplementation with 0.2%or 1.0%TA(P<0.05).Adding 0.2%TA to diet significantly decreased crypt depth,increased villus height/crypt depth ratio in the duodenum(P<0.05),and dietary 1.0%TA tended to decrease crypt depth(P<0.10)and significantly decreased villus height(P<0.05)of the ***,lower malondialdehyde content in the ileum was detected in the pigs fed 1.0%TA(P<0.05).In the duodenum,both 0.2%and 1.0%TA groups had higher occludin(OCLN)mRNA and 0.2%TA group had higher zonula occludens-2(ZO-2)level(P<0.05).Meanwhile,dietary 1.0%TA supplementation tended to up-regulate OCLN mRNA levels in the jejunum(P<0.10)and 0.2%TA supplementation tended to up-regulate zonula occludens-1(ZO-1)mRNA levels in the ileum(P<0.10).Conclusion:In conclusion,dietary supplementation of 0.2%or 1.0%TA could effectively alleviate postweaning diarrhea without altering growth performance in weaned piglets,wh

关键词： Intestine barrier Post-weaning diarrhea Tannic acid Weaned piglets

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