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癌变．畸变．突变 2001年第4期13卷 205-206页

作者： Donald MacPhee radiobiology department Radiation

Molecular Biology as a Toolin Cancer Epidemiology

关键词：癌症流行病学分子生物学基因检测

Curcumin from Jianghuang (Rhizoma Curcumae Longae) protects against exposure to ultraviolet B by antioxidation and attenuating mitochondrion-dependent apoptosis

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Journal of Traditional Chinese Medicine 2020年第5期40卷 782-791页

作者： Hou Jiguang Fang Fang Kang Shunai Wang Zhicheng Yang Yanming department of Radiotherapy Second Hospital of Jilin UniversityChangchun 130041China NHC Key laboratory of radiobiology(Jilin University) School of Public HealthJilin UniversityChangchun 130021China

OBJECTIVE:To investigate the protective effect of curcumin extracted from Jianghuang(Rhizoma Curcumae Longae)against ultraviolet B(UVB)and the possible ***:Effects of curcumin were detected in vivo and in *** changes of white guinea pig skin were assessed by hematoxylin and eosin *** CaT cell proliferation was measured by 3-[4,5-dimethylthylthiazol-2-yl]-2,5 diphenyltetrazolium broide(MTT)*** cell cycle distribution,apoptotic rate,level of reactive oxygen species(ROS),mitochondrial membrane potential,and intracellullar calcium ion concentration of Ha CaT cells were detected by flow *** levels in skin tissues and Ha Cat cells were measured by biochemical ***:UVB inhibited in vitro cell proliferation by inducing G2/M arrest,increasing ROS,apoptosis,and necrosis,and decreasing B-cell lymphoma-2,and increasing Bax,cytochrome c,and caspase-3 ***:Curcumin blocks the effects of UVB by reducing ROS and apoptosis,and reversing UVB-induced changes in the expression of apoptotic *** mitochondrial pathway is involved in curcumin-regulated apoptosis.

关键词： Ultraviolet rays Curcumin Antioxidants Apoptosis Mitochondria

DNA damage repair:historical perspectives,mechanistic pathways and clinical translation for targeted cancer therapy

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2391-2425页

作者： Ruixue Huang Ping-Kun Zhou department of Occupational and Environmental Health Xiangya School of Public HealthCentral South UniversityChangshaHunanChina department of Radiation Biology Beijing Key Laboratory for RadiobiologyBeijing Institute of Radiation MedicineAMMSBeijingChina

Genomic instability is the hallmark of various cancers with the increasing accumulation of DNA *** application of radiotherapy and chemotherapy in cancer treatment is typically based on this property of ***,the adverse effects including normal tissues injury are also accompanied by the radiotherapy and *** cancer therapy has the potential to suppress cancer cells'DNA damage response through tailoring therapy to cancer patients lacking specific DNA damage response ***,understanding the broader role of DNA damage repair in cancers has became a basic and attractive strategy for targeted cancer therapy,in particular,raising novel hypothesis or theory in this field on the basis of previous scientists'findings would be important for future promising druggable emerging *** this review,we first illustrate the timeline steps for the understanding the roles of DNA damage repair in the promotion of cancer and cancer therapy developed,then we summarize the mechanisms regarding DNA damage repair associated with targeted cancer therapy,highlighting the specific proteins behind targeting DNA damage repair that initiate functioning abnormally duo to extrinsic harm by environmental DNA damage factors,also,the DNA damage baseline drift leads to the harmful intrinsic targeted cancer *** addition,clinical therapeutic drugs for DNA damage and repair including therapeutic effects,as well as the strategy and scheme of relative clinical trials were intensive *** on this background,we suggest two hypotheses,namely"environmental gear selection”to describe DNA damage repair pathway evolution,and"DNA damage baseline drift”,which may play a magnified role in mediating repair during cancer *** two new hypothesis would shed new light on targeted cancer therapy,provide a much better or more comprehensive holistic view and also promote the development of new research direction and new overcoming strategies for

关键词： damage clinical targeted

Developmental Toxicity in Mice Following Paternal Exposure to Di-N-Butyl-Phthalate (DBP)

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Biomedical and Environmental Sciences 2011年第5期24卷 569-578页

作者： Malgorzata M.DOBRZY SKA Ewa J.TYRKIEL Krzysztof A.PACHOCKI department of Radiation Protection and radiobiology National Institute of Public Health-National Institute of Hygiene00-791 WarsawPoland department of Environmental Toxicology National Institute of Public Health-National Institute of Hygiene00-791 WarsawPoland

Objective The aim of the present study was to investigate the effects of paternal Di‐N‐butyl‐phthalate （DBP） exposure pre‐ and postnatally on F1 generation offspring,and prenatally on F2 generation *** Male mice were exposed to either 500 mg/kg or 2 000 mg/kg of DBP for 8 weeks,and mated with non‐exposed ***‐quarters of the females were sacrificed a day prior to parturition,and examined for the number of living and dead implantations,and incidence of gross *** from the remaining females were assessed for developmental markers,growth parameters,as well as sperm quantity and *** There were no changes in the fertility of parents and in intrauterine development of the *** of DBP‐exposed males demonstrated growth‐*** paternal exposure to 500 mg/kg bw of DBP,there were almost twice the number of males than females born in the F1 generation.F1 generation females had a 2.5‐day delay in vaginal *** exposure to 2 000 mg/kg bw of DBP increased the incidence of sperm head malformations in F1 generation males;however,there were no changes in the fertility and viability of foetuses in the F2 *** Paternal DBP exposure may disturb the sex ratio of the offspring,delay female sexual maturation,and deteriorate the sperm quality of F1 generation males.

关键词： Di‐n‐butyl phthalate Paternal exposure Pre‐ and postnatal effects Male‐mediated developmental toxicity

PET/CT and Hypo-Fractionated Radiotherapy of Patients with Head and Neck Cancer

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International Journal of Clinical Medicine 2018年第10期9卷 751-759页

作者： Misleidy Nápoles Morales Aldo Martínez Ramirez Juan P. Oliva Glez Claudia González Espinosa Joaquín González González Carlos Fabián Calderón Marín Waldo Quesada Cepero Fanny López Reina Rubén Elzaurdín Mora department of Radiotherapy Institute of Oncology and Radiobiology Havana Cuba Nuclear Medicine department and Molecular Imaging Institute of Oncology and Radiobiology Havana Cuba Radiotherapy department Medical Researchers Center Havana Cuba

Positron emission tomography/Computer tomography (PET/CT) is a multimodality imaging diagnostic technique that analyzes the uptake and retention of different radiopharmaceuticals by cells providing metabolic information on biochemical processes. PET/CT has been used for radiotherapy planning, providing useful information to the Radio-oncologist about the localization, size and metabolic activity of tumor lesions. In this paper, we show advantages of the 18F-FDG PET/CT respect to simple CT imaging for target volume delineation in patients with diagnosis of Squamous Head and Neck Carcinoma that has been scheduled to undergo a hypofractionated radiotherapy treatment. On ten studied patients, the target volume defined from PET/CT images was less extensive than those defined from simple CT images. In six patients the target volume was significantly less extensive and in two of them a new lymph node disease was reported, re-staging and corresponding target volume was also delineated with less extensive margins from PET/CT images. A greater accuracy in delineating the volumes and improving the distribution of doses in the planning of the radiant treatment in these patients was possible, allowing a high precision in the delivery of the prescribed dose to the target volume diminishing the maximum dose to the adjacent healthy tissues. In conclusion we show that the use of 18F-FDG PET/CT was superior than the simple CT as the primary modality of imaging for hypofractioned radiotherapy treatment planning in patients with Squamous Head and Neck Carcinoma.

关键词： PET-CT Hypofractionated Radiotherapy Head and Neck Cancer

Increased Levels of p53 and PARP-1 in EL-4 Cells Probably Related with the Immune Adaptive Response Induced by Low Dose Ionizing Radiation in vitro

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Biomedical and Environmental Sciences 2010年第6期23卷 487-495页

作者： GUANG-HUI CHENG NING WU DE-Fu JIANG HONG-GUANG ZHAO QIAN ZHANG JIAN-FENG WANG SHou-LIANG GONG department of Radiotherapy China-Japan Union Hospital Jilin University Changchun 130033 Jilin China Key Laboratory of radiobiology Ministry of Health (School of Public Health Jilin University) Changchun 130021 Jilin China department of Nuclear Medicine the First Hospital Jilin University Changchun 130021 Jilin China

Objective This paper is to explore the DNA repair mechanism of immune adaptive response （AR） induced by low dose radiation （LDR）, the changes of mRNA levels and protein expressions of p53, ATM, DNA-PK catalytic subunit （DNA-PKcs） and PARP-1 genes in the LDR-induced AR in EL-4 cells. Methods The apoptosis and cell cycle progression of EL-4 cells were detected by flow cytometry in 12 h after the cells received the pre-exposure of 0.075 Gy X-rays （inductive dose, D 1） and the succeeding high dose irradiation （challenge dose, D2; 1.0, 1.5, and 2.0 Gy X-rays, respectively） with or without wortmannin （inhibitor of ATM and DNA-PK） and 3-aminobenzamid （inhibitor of PARP-1）. And the protein expressions and mRNA levels related to these genes were detected with flow cytometry and reverse transcription-polymerase chain reaction in 12 h after irradiation with D2. Results The mRNA and protein expressions of p53 and PARP-1 in EL-4 cells in the D1 ＋ D2 groups were much lower than those in the D2 groups, and those of PARP-1 in the 3-AB ＋ D2 and the 3-AB ＋ D1 ＋ D2 groups were much lower than those in the D2 and the D1 ＋ D2 groups. The percentage of apoptotic EL-4 cells in the 3-AB ＋ D1 ＋ D2 groups was much higher than that in the D1 ＋ D2 groups, that in the G0/G1 and the G2 ＋ M phases was much higher, and that in the S phase were much lower. Although the ATM and DNA-PKcs mRNA and protein expressions in wortmannin ＋ D1 ＋ D2 groups were much lower than those in the D1 ＋ D2 groups, there were no significant changes in the apoptosis and cell cycle progression between the wortmannin ＋ D1 ＋ D2 and the D1 ＋ D2 groups. Conclusion PARP-1 and p53 might play important roles in AR induced by LDR.

关键词： Low dose radiation (LDR) Adaptive response (AR) DNA repair

Space Life Science of China in 2013

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空间科学学报 2014年第5期34卷 747-756页

作者： LEI Runhong ZHOU Guangming MA Hong ZHUANG Fengyuan DENG Yulin School of Life Science Beijing Institute of Technology department of Space radiobiology Key Laboratory of Heavy Ion Radiation Biology and MedicineInstitute of Modern Physics Chinese Academy of Sciences

In the past two years, space life science research in China is characterized by a wide area of basic researches for providing foundation for the future China Space Station. The effect of microgravity and radiation was further studied from physiology phenomena to the level of bio-molecule mechanisms. Chinese space life science is maturing in a new era of comprehensive ***, we review and summarize researches on space life sciences which were contributed by Chinese scientists.

关键词： Space life sciences Simulated microgravity Ionizing radiation

Effects of 3-aminobenzamide on poly(ADP-ribose)polymerase expression,apoptosis and cell cycle progression of HeLa cells after X-ray irradiation

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Frontiers of Medicine 2008年第2期2卷 204-206页

作者： Xiang DU Hongguang ZHAO Wen WANG Wei GUO Shouliang GONG Key Laboratory of radiobiology of Ministry of Health School of Public HealthJilin UniversityChangchun 130021China department of Toxicology School of Public HealthJilin UniversityChangchun 130021China

The aim of this paper is to study the changes of apoptosis and cell cycle progression in HeLa cells after the poly(ADP-ribose)polymerase(PARP)was inhibited by its inhibitor 3-aminobenzamide(3-AB)and the mechan-isms of PARP action on HeLa cells damaged by *** cytometry(FCM)was used to examine the PARP expression and the percentage of apoptotic cells and cell cycle *** percentage of HeLa cells with positive expression of PARP protein 2,4,8 and 12 h after administrated with 3-AB was significantly lower than that of the control(P<0.01).The percentages of apoptotic cells in the 3-AB plus irradiation group at the time points of 2,8,12 and 24 h after 2 Gy irradiation were higher than that in the irradiation group(P<0.01 or P<0.05)and the percentage of G2 cells decreased signifi-cantly(P<0.01 or P<0.05).It indicates that 3-AB can rapidly inhibit PARP expression of HeLa cells,promote cell apoptosis and block G2 arrest induced by irradiation.

关键词： poly(ADP-ribose)polymerase ADP ribose transferases 3-aminobenzamide ionizing irradiation

Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

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Biomedical and Environmental Sciences 2015年第8期28卷 549-557页

作者： DU Ya Rong PAN Dong CHEN Ya Xiong XUE Gang REN Zhen Xin LI Xiao Man ZHANG Shi Chuan HU Bu Rong Gansu Key laboratory of Space radiobiology Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences Institute of Modern Physics Chinese Academy of Sciences University of Chinese Academy of Sciences department of Radiation Oncology Sichuan Cancer Hospital

Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition. Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture. Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs’ potential of cellular transformation was lower after irradiation by soft agar assay. Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress.

关键词： Adipose-derived stem cells Three-dimensional cell culture Irradiation response High-and low-LET irradiation

DNA damage responses in cancer stem cells: Implications for cancer therapeutic strategies

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World Journal of Biological Chemistry 2015年第3期6卷 57-64页

作者： Qi-En Wang Division of radiobiology Department of Radiology the Ohio State University Wexner Medical Center

The identification of cancer stem cells(CSCs) that are responsible for tumor initiation, growth, metastasis, and therapeutic resistance might lead to a new thinking on cancer treatments. Similar to stem cells,CSCs also display high resistance to radiotherapy and chemotherapy with genotoxic agents. Thus, conventional therapy may shrink the tumor volume but cannot eliminate cancer. Eradiation of CSCs represents a novel therapeutic strategy. CSCs possess a highly efficient DNA damage response(DDR) system, which is considered as a contributor to the resistance of these cells from exposures to DNA damaging agents. Targeting of enhanced DDR in CSCs is thus proposed to facilitate the eradication of CSCs by conventional therapeutics. To achieve this aim, a better understanding of the cellular responses to DNA damage in CSCs is needed. In addition to the protein kinases and enzymes that are involved in DDR, other processes that affect the DDR including chromatin remodeling should also be explored.

关键词： Cancer stem cell DNA damage response DNA repair Cancer therapy