Increased Levels of p53 and PARP-1 in EL-4 Cells Probably Related with the Immune Adaptive Response Induced by Low Dose Ionizing Radiation in vitro

作     者：GUANG-HUI CHENG NING WU DE-Fu JIANG HONG-GUANG ZHAO QIAN ZHANG JIAN-FENG WANG SHou-LIANG GONG GUANG-HUI CHENG*,^,2,NING WU,^,2,DE-FU JIANG*,HONG-GUANG ZHAO #,QIAN ZHANG*,JIAN-FENG WANG,^,AND SHOU-LIANG GONG ^,,3 *Department of Radiotherapy,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China;^ Key Laboratory of Radiobiology,Ministry of Health (School of Public Health,Jilin University),Changchun 130021,Jilin,China;# Department of Nuclear Medicine,the First Hospital,Jilin University,Changchun 130021,Jilin,China

作者机构：Department of Radiotherapy China-Japan Union Hospital Jilin University Changchun 130033 Jilin China Key Laboratory of Radiobiology Ministry of Health (School of Public Health Jilin University) Changchun 130021 Jilin China Department of Nuclear Medicine the First Hospital Jilin University Changchun 130021 Jilin China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2010年第23卷第6期

页      面：487-495页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(30570546 and 30870747) the Natural Science Foundation of Jilin Province (20090458 and 201015183) the Young Teachers Innovative Foundation of Jilin University(421010043430) the Young Scholars Research Foundation Program of China-Japan Union Hospital(2009) 

主　　题：Low dose radiation (LDR) Adaptive response (AR) DNA repair 

摘      要：Objective This paper is to explore the DNA repair mechanism of immune adaptive response （AR） induced by low dose radiation （LDR）, the changes of mRNA levels and protein expressions of p53, ATM, DNA-PK catalytic subunit （DNA-PKcs） and PARP-1 genes in the LDR-induced AR in EL-4 cells. Methods The apoptosis and cell cycle progression of EL-4 cells were detected by flow cytometry in 12 h after the cells received the pre-exposure of 0.075 Gy X-rays （inductive dose, D 1） and the succeeding high dose irradiation （challenge dose, D2; 1.0, 1.5, and 2.0 Gy X-rays, respectively） with or without wortmannin （inhibitor of ATM and DNA-PK） and 3-aminobenzamid （inhibitor of PARP-1）. And the protein expressions and mRNA levels related to these genes were detected with flow cytometry and reverse transcription-polymerase chain reaction in 12 h after irradiation with D2. Results The mRNA and protein expressions of p53 and PARP-1 in EL-4 cells in the D1 ＋ D2 groups were much lower than those in the D2 groups, and those of PARP-1 in the 3-AB ＋ D2 and the 3-AB ＋ D1 ＋ D2 groups were much lower than those in the D2 and the D1 ＋ D2 groups. The percentage of apoptotic EL-4 cells in the 3-AB ＋ D1 ＋ D2 groups was much higher than that in the D1 ＋ D2 groups, that in the G0/G1 and the G2 ＋ M phases was much higher, and that in the S phase were much lower. Although the ATM and DNA-PKcs mRNA and protein expressions in wortmannin ＋ D1 ＋ D2 groups were much lower than those in the D1 ＋ D2 groups, there were no significant changes in the apoptosis and cell cycle progression between the wortmannin ＋ D1 ＋ D2 and the D1 ＋ D2 groups. Conclusion PARP-1 and p53 might play important roles in AR induced by LDR.